Cadmium as an endocrine disruptor: Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin
- Autores
- Jiménez Ortega, Vanesa; Cano Barquilla, Pilar; Fernández Mateos, Pilar; Cardinali, Daniel Pedro; Esquifino, Ana I.
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- To examine the effect of a low dose of cadmium (Cd) as an endocrine disruptor, male Wistar rats received CdCl2 (5 ppm Cd) in drinking water or drinking water alone. After 1 month, the rats were euthanized at one of six time intervals around the clock and the 24-h pattern of adenohypophysial prolactin (PRL) synthesis and release, lipid peroxidation, and redox enzyme and metallothionein (MT) gene expression was examined. Cd suppressed 24-h rhythmicity in expression of the PRL gene and in circulating PRL by increasing them at early photophase only, in correlation with an augmented pituitary lipid peroxidation and redox enzyme expression. CdCl2 treatment effectively disrupted the 24-h variation in expression of every pituitary parameter tested except for MT-3. In a second experiment the effect of melatonin (3 μg/ml in drinking water) was assessed at early photophase, the time of maximal endocrine-disrupting effect of Cd. Melatonin treatment blunted the effect of Cd on PRL synthesis and release, decreased Cd-induced lipid peroxidation, and counteracted the effect of Cd on expression of most redox enzymes. A third experiment was performed to examine whether melatonin could counteract Cd-induced changes in the 24-h pattern of pituitary circadian clock gene expression and plasma PRL, luteinizing hormone (LH), thyrotropin (TSH), and corticosterone levels. Rats receiving CdCl2 exhibited a suppressed daily rhythm of Clock expression and a significant disruption in daily rhythms of pituitary Bmal1, Per1, Per2, Cry1, and Cry2. The coadministration of melatonin restored rhythmicity in Clock and Bmal1 expression but shifted the maxima in pituitary Per1, Cry1, and Cry2 expression to the scotophase. Melatonin also counteracted the effect of Cd on 24-h rhythmicity of circulating PRL, LH, TSH, and corticosterone. The results highlight the occurrence of a significant endocrine disruptor effect of a low dose of Cd. Generally melatonin counteracted the effects of Cd and ameliorated partially the circadian disruption caused by the pollutant.
Fil: Jiménez Ortega, Vanesa. No especifíca;
Fil: Cano Barquilla, Pilar. No especifíca;
Fil: Fernández Mateos, Pilar. Universidad Complutense de Madrid; España
Fil: Cardinali, Daniel Pedro. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Esquifino, Ana I.. No especifíca; - Materia
-
CADMIUM
CIRCADIAN RHYTHMS
CORTICOSTERONE
FREE RADICALS
GENE EXPRESSION
LH
MELATONIN
PROLACTIN
REDOX STATE
TSH - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/190140
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
spelling |
Cadmium as an endocrine disruptor: Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatoninJiménez Ortega, VanesaCano Barquilla, PilarFernández Mateos, PilarCardinali, Daniel PedroEsquifino, Ana I.CADMIUMCIRCADIAN RHYTHMSCORTICOSTERONEFREE RADICALSGENE EXPRESSIONLHMELATONINPROLACTINREDOX STATETSHhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3To examine the effect of a low dose of cadmium (Cd) as an endocrine disruptor, male Wistar rats received CdCl2 (5 ppm Cd) in drinking water or drinking water alone. After 1 month, the rats were euthanized at one of six time intervals around the clock and the 24-h pattern of adenohypophysial prolactin (PRL) synthesis and release, lipid peroxidation, and redox enzyme and metallothionein (MT) gene expression was examined. Cd suppressed 24-h rhythmicity in expression of the PRL gene and in circulating PRL by increasing them at early photophase only, in correlation with an augmented pituitary lipid peroxidation and redox enzyme expression. CdCl2 treatment effectively disrupted the 24-h variation in expression of every pituitary parameter tested except for MT-3. In a second experiment the effect of melatonin (3 μg/ml in drinking water) was assessed at early photophase, the time of maximal endocrine-disrupting effect of Cd. Melatonin treatment blunted the effect of Cd on PRL synthesis and release, decreased Cd-induced lipid peroxidation, and counteracted the effect of Cd on expression of most redox enzymes. A third experiment was performed to examine whether melatonin could counteract Cd-induced changes in the 24-h pattern of pituitary circadian clock gene expression and plasma PRL, luteinizing hormone (LH), thyrotropin (TSH), and corticosterone levels. Rats receiving CdCl2 exhibited a suppressed daily rhythm of Clock expression and a significant disruption in daily rhythms of pituitary Bmal1, Per1, Per2, Cry1, and Cry2. The coadministration of melatonin restored rhythmicity in Clock and Bmal1 expression but shifted the maxima in pituitary Per1, Cry1, and Cry2 expression to the scotophase. Melatonin also counteracted the effect of Cd on 24-h rhythmicity of circulating PRL, LH, TSH, and corticosterone. The results highlight the occurrence of a significant endocrine disruptor effect of a low dose of Cd. Generally melatonin counteracted the effects of Cd and ameliorated partially the circadian disruption caused by the pollutant.Fil: Jiménez Ortega, Vanesa. No especifíca;Fil: Cano Barquilla, Pilar. No especifíca;Fil: Fernández Mateos, Pilar. Universidad Complutense de Madrid; EspañaFil: Cardinali, Daniel Pedro. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Esquifino, Ana I.. No especifíca;Elsevier Science Inc.2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/190140Jiménez Ortega, Vanesa; Cano Barquilla, Pilar; Fernández Mateos, Pilar; Cardinali, Daniel Pedro; Esquifino, Ana I.; Cadmium as an endocrine disruptor: Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin; Elsevier Science Inc.; Free Radical Biology and Medicine; 53; 12; 12-2012; 2287-22970891-5849CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0891584912017649info:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2012.10.533info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:34:23Zoai:ri.conicet.gov.ar:11336/190140instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:34:23.92CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Cadmium as an endocrine disruptor: Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin |
title |
Cadmium as an endocrine disruptor: Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin |
spellingShingle |
Cadmium as an endocrine disruptor: Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin Jiménez Ortega, Vanesa CADMIUM CIRCADIAN RHYTHMS CORTICOSTERONE FREE RADICALS GENE EXPRESSION LH MELATONIN PROLACTIN REDOX STATE TSH |
title_short |
Cadmium as an endocrine disruptor: Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin |
title_full |
Cadmium as an endocrine disruptor: Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin |
title_fullStr |
Cadmium as an endocrine disruptor: Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin |
title_full_unstemmed |
Cadmium as an endocrine disruptor: Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin |
title_sort |
Cadmium as an endocrine disruptor: Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin |
dc.creator.none.fl_str_mv |
Jiménez Ortega, Vanesa Cano Barquilla, Pilar Fernández Mateos, Pilar Cardinali, Daniel Pedro Esquifino, Ana I. |
author |
Jiménez Ortega, Vanesa |
author_facet |
Jiménez Ortega, Vanesa Cano Barquilla, Pilar Fernández Mateos, Pilar Cardinali, Daniel Pedro Esquifino, Ana I. |
author_role |
author |
author2 |
Cano Barquilla, Pilar Fernández Mateos, Pilar Cardinali, Daniel Pedro Esquifino, Ana I. |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
CADMIUM CIRCADIAN RHYTHMS CORTICOSTERONE FREE RADICALS GENE EXPRESSION LH MELATONIN PROLACTIN REDOX STATE TSH |
topic |
CADMIUM CIRCADIAN RHYTHMS CORTICOSTERONE FREE RADICALS GENE EXPRESSION LH MELATONIN PROLACTIN REDOX STATE TSH |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
To examine the effect of a low dose of cadmium (Cd) as an endocrine disruptor, male Wistar rats received CdCl2 (5 ppm Cd) in drinking water or drinking water alone. After 1 month, the rats were euthanized at one of six time intervals around the clock and the 24-h pattern of adenohypophysial prolactin (PRL) synthesis and release, lipid peroxidation, and redox enzyme and metallothionein (MT) gene expression was examined. Cd suppressed 24-h rhythmicity in expression of the PRL gene and in circulating PRL by increasing them at early photophase only, in correlation with an augmented pituitary lipid peroxidation and redox enzyme expression. CdCl2 treatment effectively disrupted the 24-h variation in expression of every pituitary parameter tested except for MT-3. In a second experiment the effect of melatonin (3 μg/ml in drinking water) was assessed at early photophase, the time of maximal endocrine-disrupting effect of Cd. Melatonin treatment blunted the effect of Cd on PRL synthesis and release, decreased Cd-induced lipid peroxidation, and counteracted the effect of Cd on expression of most redox enzymes. A third experiment was performed to examine whether melatonin could counteract Cd-induced changes in the 24-h pattern of pituitary circadian clock gene expression and plasma PRL, luteinizing hormone (LH), thyrotropin (TSH), and corticosterone levels. Rats receiving CdCl2 exhibited a suppressed daily rhythm of Clock expression and a significant disruption in daily rhythms of pituitary Bmal1, Per1, Per2, Cry1, and Cry2. The coadministration of melatonin restored rhythmicity in Clock and Bmal1 expression but shifted the maxima in pituitary Per1, Cry1, and Cry2 expression to the scotophase. Melatonin also counteracted the effect of Cd on 24-h rhythmicity of circulating PRL, LH, TSH, and corticosterone. The results highlight the occurrence of a significant endocrine disruptor effect of a low dose of Cd. Generally melatonin counteracted the effects of Cd and ameliorated partially the circadian disruption caused by the pollutant. Fil: Jiménez Ortega, Vanesa. No especifíca; Fil: Cano Barquilla, Pilar. No especifíca; Fil: Fernández Mateos, Pilar. Universidad Complutense de Madrid; España Fil: Cardinali, Daniel Pedro. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Esquifino, Ana I.. No especifíca; |
description |
To examine the effect of a low dose of cadmium (Cd) as an endocrine disruptor, male Wistar rats received CdCl2 (5 ppm Cd) in drinking water or drinking water alone. After 1 month, the rats were euthanized at one of six time intervals around the clock and the 24-h pattern of adenohypophysial prolactin (PRL) synthesis and release, lipid peroxidation, and redox enzyme and metallothionein (MT) gene expression was examined. Cd suppressed 24-h rhythmicity in expression of the PRL gene and in circulating PRL by increasing them at early photophase only, in correlation with an augmented pituitary lipid peroxidation and redox enzyme expression. CdCl2 treatment effectively disrupted the 24-h variation in expression of every pituitary parameter tested except for MT-3. In a second experiment the effect of melatonin (3 μg/ml in drinking water) was assessed at early photophase, the time of maximal endocrine-disrupting effect of Cd. Melatonin treatment blunted the effect of Cd on PRL synthesis and release, decreased Cd-induced lipid peroxidation, and counteracted the effect of Cd on expression of most redox enzymes. A third experiment was performed to examine whether melatonin could counteract Cd-induced changes in the 24-h pattern of pituitary circadian clock gene expression and plasma PRL, luteinizing hormone (LH), thyrotropin (TSH), and corticosterone levels. Rats receiving CdCl2 exhibited a suppressed daily rhythm of Clock expression and a significant disruption in daily rhythms of pituitary Bmal1, Per1, Per2, Cry1, and Cry2. The coadministration of melatonin restored rhythmicity in Clock and Bmal1 expression but shifted the maxima in pituitary Per1, Cry1, and Cry2 expression to the scotophase. Melatonin also counteracted the effect of Cd on 24-h rhythmicity of circulating PRL, LH, TSH, and corticosterone. The results highlight the occurrence of a significant endocrine disruptor effect of a low dose of Cd. Generally melatonin counteracted the effects of Cd and ameliorated partially the circadian disruption caused by the pollutant. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/190140 Jiménez Ortega, Vanesa; Cano Barquilla, Pilar; Fernández Mateos, Pilar; Cardinali, Daniel Pedro; Esquifino, Ana I.; Cadmium as an endocrine disruptor: Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin; Elsevier Science Inc.; Free Radical Biology and Medicine; 53; 12; 12-2012; 2287-2297 0891-5849 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/190140 |
identifier_str_mv |
Jiménez Ortega, Vanesa; Cano Barquilla, Pilar; Fernández Mateos, Pilar; Cardinali, Daniel Pedro; Esquifino, Ana I.; Cadmium as an endocrine disruptor: Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin; Elsevier Science Inc.; Free Radical Biology and Medicine; 53; 12; 12-2012; 2287-2297 0891-5849 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0891584912017649 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2012.10.533 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science Inc. |
publisher.none.fl_str_mv |
Elsevier Science Inc. |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613064035401728 |
score |
13.070432 |