Seasonal variations of gonadotropins in the pars distalis male viscacha pituitary: Effect of chronic melatonin treatment
- Autores
- Filippa, Veronica Palmira; Penissi, Alicia Beatriz; Mohamed, Fabian Heber
- Año de publicación
- 2005
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The gonadotropes, LH and FSH cells, were immunohistochemically identified in the pituitary pars distalis of the adult male viscacha (Lagostomus maximus maximus) using specific antibodies against hLHb and hFSHb with the streptavidin-biotin-peroxidase complex. The distribution, size and percentage immunopositive area of these cells were analyzed by image analysis in viscachas captured during the annual reproductive cycle and after the chronic administration of melatonin. The LHb and FSHb cells showed seasonal changes in the distribution, size and percentage immunopositive area. The LHb cells were found widely distributed throughout the pars distalis during the reproductive period, and they were found in the ventro-medial region in the pars distalis during the gonadal regression and gonadal recovery periods. The LHb cells reached the largest size and immunopositive area during the reproductive period and the smallest size and immunopositive area during the gonadal regression period. The FSHb cells were found in the ventromedial region during reproductive and gonadal regression periods. The FSHb cells were found widely distributed throughout the pars distalis during the gonadal recovery period when they showed the maximum percentage immunopositive area. A decrease in the size of LHb and FSHb cells was observed after the chronic administration of melatonin. Moreover, it produces a decrease in the immunopositive area occupied by the LHb cells but not in the immunopositive area occupied by the FSHb cells. Our results show great activity of LHb and FSHb cells in different moments of the annual reproductive cycle demonstrating that these cells do not secrete in parallel. Moreover, melatonin acts differentially on the activity of the gonadotrope cells.
Fil: Filippa, Veronica Palmira. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Penissi, Alicia Beatriz. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mendoza. Instituto de Histologia y Embriologia de Mendoza Dr. Mario H. Burgos. Grupo Vinculado de Investigacion y Desarrollo Biotecnologico Aplicado Al Diagnostico Al Ihem | Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Histologia y Embriologia de Mendoza Dr. Mario H. Burgos. Grupo Vinculado de Investigacion y Desarrollo Biotecnologico Aplicado Al Diagnostico Al Ihem.; Argentina
Fil: Mohamed, Fabian Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina - Materia
-
PITUITARY
LH
FSH
IMMUNOHISTOCHEMISTRY
VISCACHA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/159582
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
spelling |
Seasonal variations of gonadotropins in the pars distalis male viscacha pituitary: Effect of chronic melatonin treatmentFilippa, Veronica PalmiraPenissi, Alicia BeatrizMohamed, Fabian HeberPITUITARYLHFSHIMMUNOHISTOCHEMISTRYVISCACHAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The gonadotropes, LH and FSH cells, were immunohistochemically identified in the pituitary pars distalis of the adult male viscacha (Lagostomus maximus maximus) using specific antibodies against hLHb and hFSHb with the streptavidin-biotin-peroxidase complex. The distribution, size and percentage immunopositive area of these cells were analyzed by image analysis in viscachas captured during the annual reproductive cycle and after the chronic administration of melatonin. The LHb and FSHb cells showed seasonal changes in the distribution, size and percentage immunopositive area. The LHb cells were found widely distributed throughout the pars distalis during the reproductive period, and they were found in the ventro-medial region in the pars distalis during the gonadal regression and gonadal recovery periods. The LHb cells reached the largest size and immunopositive area during the reproductive period and the smallest size and immunopositive area during the gonadal regression period. The FSHb cells were found in the ventromedial region during reproductive and gonadal regression periods. The FSHb cells were found widely distributed throughout the pars distalis during the gonadal recovery period when they showed the maximum percentage immunopositive area. A decrease in the size of LHb and FSHb cells was observed after the chronic administration of melatonin. Moreover, it produces a decrease in the immunopositive area occupied by the LHb cells but not in the immunopositive area occupied by the FSHb cells. Our results show great activity of LHb and FSHb cells in different moments of the annual reproductive cycle demonstrating that these cells do not secrete in parallel. Moreover, melatonin acts differentially on the activity of the gonadotrope cells.Fil: Filippa, Veronica Palmira. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Penissi, Alicia Beatriz. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mendoza. Instituto de Histologia y Embriologia de Mendoza Dr. Mario H. Burgos. Grupo Vinculado de Investigacion y Desarrollo Biotecnologico Aplicado Al Diagnostico Al Ihem | Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Histologia y Embriologia de Mendoza Dr. Mario H. Burgos. Grupo Vinculado de Investigacion y Desarrollo Biotecnologico Aplicado Al Diagnostico Al Ihem.; ArgentinaFil: Mohamed, Fabian Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaSocietà Italiana di Istochimica2005-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/159582Filippa, Veronica Palmira; Penissi, Alicia Beatriz; Mohamed, Fabian Heber; Seasonal variations of gonadotropins in the pars distalis male viscacha pituitary: Effect of chronic melatonin treatment; Società Italiana di Istochimica; European Journal of Histochemistry; 49; 3; 12-2005; 291-3001121-760X2038-8306CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ejh.it/index.php/ejh/article/view/956info:eu-repo/semantics/altIdentifier/doi/10.4081/956info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:44:50Zoai:ri.conicet.gov.ar:11336/159582instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:44:50.336CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Seasonal variations of gonadotropins in the pars distalis male viscacha pituitary: Effect of chronic melatonin treatment |
title |
Seasonal variations of gonadotropins in the pars distalis male viscacha pituitary: Effect of chronic melatonin treatment |
spellingShingle |
Seasonal variations of gonadotropins in the pars distalis male viscacha pituitary: Effect of chronic melatonin treatment Filippa, Veronica Palmira PITUITARY LH FSH IMMUNOHISTOCHEMISTRY VISCACHA |
title_short |
Seasonal variations of gonadotropins in the pars distalis male viscacha pituitary: Effect of chronic melatonin treatment |
title_full |
Seasonal variations of gonadotropins in the pars distalis male viscacha pituitary: Effect of chronic melatonin treatment |
title_fullStr |
Seasonal variations of gonadotropins in the pars distalis male viscacha pituitary: Effect of chronic melatonin treatment |
title_full_unstemmed |
Seasonal variations of gonadotropins in the pars distalis male viscacha pituitary: Effect of chronic melatonin treatment |
title_sort |
Seasonal variations of gonadotropins in the pars distalis male viscacha pituitary: Effect of chronic melatonin treatment |
dc.creator.none.fl_str_mv |
Filippa, Veronica Palmira Penissi, Alicia Beatriz Mohamed, Fabian Heber |
author |
Filippa, Veronica Palmira |
author_facet |
Filippa, Veronica Palmira Penissi, Alicia Beatriz Mohamed, Fabian Heber |
author_role |
author |
author2 |
Penissi, Alicia Beatriz Mohamed, Fabian Heber |
author2_role |
author author |
dc.subject.none.fl_str_mv |
PITUITARY LH FSH IMMUNOHISTOCHEMISTRY VISCACHA |
topic |
PITUITARY LH FSH IMMUNOHISTOCHEMISTRY VISCACHA |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The gonadotropes, LH and FSH cells, were immunohistochemically identified in the pituitary pars distalis of the adult male viscacha (Lagostomus maximus maximus) using specific antibodies against hLHb and hFSHb with the streptavidin-biotin-peroxidase complex. The distribution, size and percentage immunopositive area of these cells were analyzed by image analysis in viscachas captured during the annual reproductive cycle and after the chronic administration of melatonin. The LHb and FSHb cells showed seasonal changes in the distribution, size and percentage immunopositive area. The LHb cells were found widely distributed throughout the pars distalis during the reproductive period, and they were found in the ventro-medial region in the pars distalis during the gonadal regression and gonadal recovery periods. The LHb cells reached the largest size and immunopositive area during the reproductive period and the smallest size and immunopositive area during the gonadal regression period. The FSHb cells were found in the ventromedial region during reproductive and gonadal regression periods. The FSHb cells were found widely distributed throughout the pars distalis during the gonadal recovery period when they showed the maximum percentage immunopositive area. A decrease in the size of LHb and FSHb cells was observed after the chronic administration of melatonin. Moreover, it produces a decrease in the immunopositive area occupied by the LHb cells but not in the immunopositive area occupied by the FSHb cells. Our results show great activity of LHb and FSHb cells in different moments of the annual reproductive cycle demonstrating that these cells do not secrete in parallel. Moreover, melatonin acts differentially on the activity of the gonadotrope cells. Fil: Filippa, Veronica Palmira. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Penissi, Alicia Beatriz. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mendoza. Instituto de Histologia y Embriologia de Mendoza Dr. Mario H. Burgos. Grupo Vinculado de Investigacion y Desarrollo Biotecnologico Aplicado Al Diagnostico Al Ihem | Universidad Nacional de Cuyo. Facultad de Ciencias Medicas. Instituto de Histologia y Embriologia de Mendoza Dr. Mario H. Burgos. Grupo Vinculado de Investigacion y Desarrollo Biotecnologico Aplicado Al Diagnostico Al Ihem.; Argentina Fil: Mohamed, Fabian Heber. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina |
description |
The gonadotropes, LH and FSH cells, were immunohistochemically identified in the pituitary pars distalis of the adult male viscacha (Lagostomus maximus maximus) using specific antibodies against hLHb and hFSHb with the streptavidin-biotin-peroxidase complex. The distribution, size and percentage immunopositive area of these cells were analyzed by image analysis in viscachas captured during the annual reproductive cycle and after the chronic administration of melatonin. The LHb and FSHb cells showed seasonal changes in the distribution, size and percentage immunopositive area. The LHb cells were found widely distributed throughout the pars distalis during the reproductive period, and they were found in the ventro-medial region in the pars distalis during the gonadal regression and gonadal recovery periods. The LHb cells reached the largest size and immunopositive area during the reproductive period and the smallest size and immunopositive area during the gonadal regression period. The FSHb cells were found in the ventromedial region during reproductive and gonadal regression periods. The FSHb cells were found widely distributed throughout the pars distalis during the gonadal recovery period when they showed the maximum percentage immunopositive area. A decrease in the size of LHb and FSHb cells was observed after the chronic administration of melatonin. Moreover, it produces a decrease in the immunopositive area occupied by the LHb cells but not in the immunopositive area occupied by the FSHb cells. Our results show great activity of LHb and FSHb cells in different moments of the annual reproductive cycle demonstrating that these cells do not secrete in parallel. Moreover, melatonin acts differentially on the activity of the gonadotrope cells. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/159582 Filippa, Veronica Palmira; Penissi, Alicia Beatriz; Mohamed, Fabian Heber; Seasonal variations of gonadotropins in the pars distalis male viscacha pituitary: Effect of chronic melatonin treatment; Società Italiana di Istochimica; European Journal of Histochemistry; 49; 3; 12-2005; 291-300 1121-760X 2038-8306 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/159582 |
identifier_str_mv |
Filippa, Veronica Palmira; Penissi, Alicia Beatriz; Mohamed, Fabian Heber; Seasonal variations of gonadotropins in the pars distalis male viscacha pituitary: Effect of chronic melatonin treatment; Società Italiana di Istochimica; European Journal of Histochemistry; 49; 3; 12-2005; 291-300 1121-760X 2038-8306 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.ejh.it/index.php/ejh/article/view/956 info:eu-repo/semantics/altIdentifier/doi/10.4081/956 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Società Italiana di Istochimica |
publisher.none.fl_str_mv |
Società Italiana di Istochimica |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846782143397625856 |
score |
12.982451 |