Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms

Autores
Jiménez Ortega, Vanesa; Barquilla, Pilar Cano; Pagano, Eleonora Samanta; Fernández Mateos, Pilar; Esquifino, Ana I.; Cardinali, Daniel Pedro
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the laboratory rat, a number of physiological parameters display seasonal changes even under constant conditions of temperature, lighting, and food availability. Since there is evidence that prolactin (PRL) is, among the endocrine signals, a major mediator of seasonal adaptations, the authors aimed to examine whether melatonin administration in drinking water resembling in length the exposure to a winter photoperiod could affect accordingly the 24-h pattern of PRL synthesis and release and some of their anterior pituitary redox state and circadian clock modulatory mechanisms. Melatonin (3gmL drinking water) or vehicle was given for 1 mo, and rats were euthanized at six time intervals during a 24-h cycle. High concentrations of melatonin (>2000 pgmL) were detected in melatonin-treated rats from beginning of scotophase (at 21:00h) to early photophase (at 09:00h) as compared with a considerably narrower high-melatonin phase observed in controls. By cosinor analysis, melatonin-treated rats had significantly decreased MESOR (24-h time-series average) values of anterior pituitary PRL gene expression and circulating PRL, with acrophases (peak time) located in the middle of the scotophase, as in the control group. Melatonin treatment disrupted the 24-h pattern of anterior pituitary gene expression of nitric oxide synthase (NOS)-1 and -2, heme oxygenase-1 and -2, glutathione peroxidase, glutathione reductase, CuZn- and Mn-superoxide dismutase, and catalase by shifting their acrophases to earlymiddle scotophase or amplifying the maxima. Only the inhibitory effect of melatonin on pituitary NOS-2 gene expression correlated temporally with inhibition of PRL production. Gene expression of metallothionein-1 and -3 showed maxima at earlymiddle photophase after melatonin treatment. The 24-h pattern of anterior pituitary lipid peroxidation did not vary after treatment. In vehicle-treated rats, Clock and Bmal1 expression peaked in the anterior pituitary at middle scotophase, whereas that of Per1 and Per2 and of Cry1 and Cry2 peaked at the middle and late photophase, respectively. Treatment with melatonin raised mean expression of anterior pituitary Per2, Cry1, and Cry2. In the case of Per1, decreased MESOR was observed, although the single significant difference found between the experimental groups when analyzed at individual time intervals was increase at early scotophase in the anterior pituitary of melatonin-treated rats. Melatonin significantly phase-delayed expression of Per1, Per2, and Cry1, also phase-delayed the plasma corticosterone circadian rhythm, and increased the amplitude of plasma corticosterone and thyrotropin rhythms. The results indicate that under prolonged duration of a daily melatonin signal, rat anterior pituitary PRL synthesis and release are depressed, together with significant changes in the redox and circadian mechanisms controlling them.
Fil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid; España
Fil: Barquilla, Pilar Cano. Universidad Complutense de Madrid; España
Fil: Pagano, Eleonora Samanta. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández Mateos, Pilar. Universidad Complutense de Madrid; España
Fil: Esquifino, Ana I.. Universidad Complutense de Madrid; España
Fil: Cardinali, Daniel Pedro. Universidad de Buenos Aires; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
CORTICOSTERONE
FREE RADICALS
LH
METALLOTHIONEINS
SEASONALITY
TESTOSTERONE
TSH
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/196970

id CONICETDig_c4c54ab14d83999da1a42b6628536c17
oai_identifier_str oai:ri.conicet.gov.ar:11336/196970
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanismsJiménez Ortega, VanesaBarquilla, Pilar CanoPagano, Eleonora SamantaFernández Mateos, PilarEsquifino, Ana I.Cardinali, Daniel PedroCORTICOSTERONEFREE RADICALSLHMETALLOTHIONEINSSEASONALITYTESTOSTERONETSHhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In the laboratory rat, a number of physiological parameters display seasonal changes even under constant conditions of temperature, lighting, and food availability. Since there is evidence that prolactin (PRL) is, among the endocrine signals, a major mediator of seasonal adaptations, the authors aimed to examine whether melatonin administration in drinking water resembling in length the exposure to a winter photoperiod could affect accordingly the 24-h pattern of PRL synthesis and release and some of their anterior pituitary redox state and circadian clock modulatory mechanisms. Melatonin (3gmL drinking water) or vehicle was given for 1 mo, and rats were euthanized at six time intervals during a 24-h cycle. High concentrations of melatonin (>2000 pgmL) were detected in melatonin-treated rats from beginning of scotophase (at 21:00h) to early photophase (at 09:00h) as compared with a considerably narrower high-melatonin phase observed in controls. By cosinor analysis, melatonin-treated rats had significantly decreased MESOR (24-h time-series average) values of anterior pituitary PRL gene expression and circulating PRL, with acrophases (peak time) located in the middle of the scotophase, as in the control group. Melatonin treatment disrupted the 24-h pattern of anterior pituitary gene expression of nitric oxide synthase (NOS)-1 and -2, heme oxygenase-1 and -2, glutathione peroxidase, glutathione reductase, CuZn- and Mn-superoxide dismutase, and catalase by shifting their acrophases to earlymiddle scotophase or amplifying the maxima. Only the inhibitory effect of melatonin on pituitary NOS-2 gene expression correlated temporally with inhibition of PRL production. Gene expression of metallothionein-1 and -3 showed maxima at earlymiddle photophase after melatonin treatment. The 24-h pattern of anterior pituitary lipid peroxidation did not vary after treatment. In vehicle-treated rats, Clock and Bmal1 expression peaked in the anterior pituitary at middle scotophase, whereas that of Per1 and Per2 and of Cry1 and Cry2 peaked at the middle and late photophase, respectively. Treatment with melatonin raised mean expression of anterior pituitary Per2, Cry1, and Cry2. In the case of Per1, decreased MESOR was observed, although the single significant difference found between the experimental groups when analyzed at individual time intervals was increase at early scotophase in the anterior pituitary of melatonin-treated rats. Melatonin significantly phase-delayed expression of Per1, Per2, and Cry1, also phase-delayed the plasma corticosterone circadian rhythm, and increased the amplitude of plasma corticosterone and thyrotropin rhythms. The results indicate that under prolonged duration of a daily melatonin signal, rat anterior pituitary PRL synthesis and release are depressed, together with significant changes in the redox and circadian mechanisms controlling them.Fil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid; EspañaFil: Barquilla, Pilar Cano. Universidad Complutense de Madrid; EspañaFil: Pagano, Eleonora Samanta. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernández Mateos, Pilar. Universidad Complutense de Madrid; EspañaFil: Esquifino, Ana I.. Universidad Complutense de Madrid; EspañaFil: Cardinali, Daniel Pedro. Universidad de Buenos Aires; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaTaylor & Francis2012-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/196970Jiménez Ortega, Vanesa; Barquilla, Pilar Cano; Pagano, Eleonora Samanta; Fernández Mateos, Pilar; Esquifino, Ana I.; et al.; Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms; Taylor & Francis; Chronobiology International; 29; 8; 8-2012; 1021-10350742-0528CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://informahealthcare.com/doi/abs/10.3109/07420528.2012.705936info:eu-repo/semantics/altIdentifier/doi/10.3109/07420528.2012.705936info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:54Zoai:ri.conicet.gov.ar:11336/196970instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:54.515CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms
title Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms
spellingShingle Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms
Jiménez Ortega, Vanesa
CORTICOSTERONE
FREE RADICALS
LH
METALLOTHIONEINS
SEASONALITY
TESTOSTERONE
TSH
title_short Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms
title_full Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms
title_fullStr Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms
title_full_unstemmed Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms
title_sort Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms
dc.creator.none.fl_str_mv Jiménez Ortega, Vanesa
Barquilla, Pilar Cano
Pagano, Eleonora Samanta
Fernández Mateos, Pilar
Esquifino, Ana I.
Cardinali, Daniel Pedro
author Jiménez Ortega, Vanesa
author_facet Jiménez Ortega, Vanesa
Barquilla, Pilar Cano
Pagano, Eleonora Samanta
Fernández Mateos, Pilar
Esquifino, Ana I.
Cardinali, Daniel Pedro
author_role author
author2 Barquilla, Pilar Cano
Pagano, Eleonora Samanta
Fernández Mateos, Pilar
Esquifino, Ana I.
Cardinali, Daniel Pedro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CORTICOSTERONE
FREE RADICALS
LH
METALLOTHIONEINS
SEASONALITY
TESTOSTERONE
TSH
topic CORTICOSTERONE
FREE RADICALS
LH
METALLOTHIONEINS
SEASONALITY
TESTOSTERONE
TSH
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In the laboratory rat, a number of physiological parameters display seasonal changes even under constant conditions of temperature, lighting, and food availability. Since there is evidence that prolactin (PRL) is, among the endocrine signals, a major mediator of seasonal adaptations, the authors aimed to examine whether melatonin administration in drinking water resembling in length the exposure to a winter photoperiod could affect accordingly the 24-h pattern of PRL synthesis and release and some of their anterior pituitary redox state and circadian clock modulatory mechanisms. Melatonin (3gmL drinking water) or vehicle was given for 1 mo, and rats were euthanized at six time intervals during a 24-h cycle. High concentrations of melatonin (>2000 pgmL) were detected in melatonin-treated rats from beginning of scotophase (at 21:00h) to early photophase (at 09:00h) as compared with a considerably narrower high-melatonin phase observed in controls. By cosinor analysis, melatonin-treated rats had significantly decreased MESOR (24-h time-series average) values of anterior pituitary PRL gene expression and circulating PRL, with acrophases (peak time) located in the middle of the scotophase, as in the control group. Melatonin treatment disrupted the 24-h pattern of anterior pituitary gene expression of nitric oxide synthase (NOS)-1 and -2, heme oxygenase-1 and -2, glutathione peroxidase, glutathione reductase, CuZn- and Mn-superoxide dismutase, and catalase by shifting their acrophases to earlymiddle scotophase or amplifying the maxima. Only the inhibitory effect of melatonin on pituitary NOS-2 gene expression correlated temporally with inhibition of PRL production. Gene expression of metallothionein-1 and -3 showed maxima at earlymiddle photophase after melatonin treatment. The 24-h pattern of anterior pituitary lipid peroxidation did not vary after treatment. In vehicle-treated rats, Clock and Bmal1 expression peaked in the anterior pituitary at middle scotophase, whereas that of Per1 and Per2 and of Cry1 and Cry2 peaked at the middle and late photophase, respectively. Treatment with melatonin raised mean expression of anterior pituitary Per2, Cry1, and Cry2. In the case of Per1, decreased MESOR was observed, although the single significant difference found between the experimental groups when analyzed at individual time intervals was increase at early scotophase in the anterior pituitary of melatonin-treated rats. Melatonin significantly phase-delayed expression of Per1, Per2, and Cry1, also phase-delayed the plasma corticosterone circadian rhythm, and increased the amplitude of plasma corticosterone and thyrotropin rhythms. The results indicate that under prolonged duration of a daily melatonin signal, rat anterior pituitary PRL synthesis and release are depressed, together with significant changes in the redox and circadian mechanisms controlling them.
Fil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid; España
Fil: Barquilla, Pilar Cano. Universidad Complutense de Madrid; España
Fil: Pagano, Eleonora Samanta. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández Mateos, Pilar. Universidad Complutense de Madrid; España
Fil: Esquifino, Ana I.. Universidad Complutense de Madrid; España
Fil: Cardinali, Daniel Pedro. Universidad de Buenos Aires; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description In the laboratory rat, a number of physiological parameters display seasonal changes even under constant conditions of temperature, lighting, and food availability. Since there is evidence that prolactin (PRL) is, among the endocrine signals, a major mediator of seasonal adaptations, the authors aimed to examine whether melatonin administration in drinking water resembling in length the exposure to a winter photoperiod could affect accordingly the 24-h pattern of PRL synthesis and release and some of their anterior pituitary redox state and circadian clock modulatory mechanisms. Melatonin (3gmL drinking water) or vehicle was given for 1 mo, and rats were euthanized at six time intervals during a 24-h cycle. High concentrations of melatonin (>2000 pgmL) were detected in melatonin-treated rats from beginning of scotophase (at 21:00h) to early photophase (at 09:00h) as compared with a considerably narrower high-melatonin phase observed in controls. By cosinor analysis, melatonin-treated rats had significantly decreased MESOR (24-h time-series average) values of anterior pituitary PRL gene expression and circulating PRL, with acrophases (peak time) located in the middle of the scotophase, as in the control group. Melatonin treatment disrupted the 24-h pattern of anterior pituitary gene expression of nitric oxide synthase (NOS)-1 and -2, heme oxygenase-1 and -2, glutathione peroxidase, glutathione reductase, CuZn- and Mn-superoxide dismutase, and catalase by shifting their acrophases to earlymiddle scotophase or amplifying the maxima. Only the inhibitory effect of melatonin on pituitary NOS-2 gene expression correlated temporally with inhibition of PRL production. Gene expression of metallothionein-1 and -3 showed maxima at earlymiddle photophase after melatonin treatment. The 24-h pattern of anterior pituitary lipid peroxidation did not vary after treatment. In vehicle-treated rats, Clock and Bmal1 expression peaked in the anterior pituitary at middle scotophase, whereas that of Per1 and Per2 and of Cry1 and Cry2 peaked at the middle and late photophase, respectively. Treatment with melatonin raised mean expression of anterior pituitary Per2, Cry1, and Cry2. In the case of Per1, decreased MESOR was observed, although the single significant difference found between the experimental groups when analyzed at individual time intervals was increase at early scotophase in the anterior pituitary of melatonin-treated rats. Melatonin significantly phase-delayed expression of Per1, Per2, and Cry1, also phase-delayed the plasma corticosterone circadian rhythm, and increased the amplitude of plasma corticosterone and thyrotropin rhythms. The results indicate that under prolonged duration of a daily melatonin signal, rat anterior pituitary PRL synthesis and release are depressed, together with significant changes in the redox and circadian mechanisms controlling them.
publishDate 2012
dc.date.none.fl_str_mv 2012-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/196970
Jiménez Ortega, Vanesa; Barquilla, Pilar Cano; Pagano, Eleonora Samanta; Fernández Mateos, Pilar; Esquifino, Ana I.; et al.; Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms; Taylor & Francis; Chronobiology International; 29; 8; 8-2012; 1021-1035
0742-0528
CONICET Digital
CONICET
url http://hdl.handle.net/11336/196970
identifier_str_mv Jiménez Ortega, Vanesa; Barquilla, Pilar Cano; Pagano, Eleonora Samanta; Fernández Mateos, Pilar; Esquifino, Ana I.; et al.; Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms; Taylor & Francis; Chronobiology International; 29; 8; 8-2012; 1021-1035
0742-0528
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://informahealthcare.com/doi/abs/10.3109/07420528.2012.705936
info:eu-repo/semantics/altIdentifier/doi/10.3109/07420528.2012.705936
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614524076818432
score 13.070432