Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms
- Autores
- Jiménez Ortega, Vanesa; Barquilla, Pilar Cano; Pagano, Eleonora Samanta; Fernández Mateos, Pilar; Esquifino, Ana I.; Cardinali, Daniel Pedro
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In the laboratory rat, a number of physiological parameters display seasonal changes even under constant conditions of temperature, lighting, and food availability. Since there is evidence that prolactin (PRL) is, among the endocrine signals, a major mediator of seasonal adaptations, the authors aimed to examine whether melatonin administration in drinking water resembling in length the exposure to a winter photoperiod could affect accordingly the 24-h pattern of PRL synthesis and release and some of their anterior pituitary redox state and circadian clock modulatory mechanisms. Melatonin (3gmL drinking water) or vehicle was given for 1 mo, and rats were euthanized at six time intervals during a 24-h cycle. High concentrations of melatonin (>2000 pgmL) were detected in melatonin-treated rats from beginning of scotophase (at 21:00h) to early photophase (at 09:00h) as compared with a considerably narrower high-melatonin phase observed in controls. By cosinor analysis, melatonin-treated rats had significantly decreased MESOR (24-h time-series average) values of anterior pituitary PRL gene expression and circulating PRL, with acrophases (peak time) located in the middle of the scotophase, as in the control group. Melatonin treatment disrupted the 24-h pattern of anterior pituitary gene expression of nitric oxide synthase (NOS)-1 and -2, heme oxygenase-1 and -2, glutathione peroxidase, glutathione reductase, CuZn- and Mn-superoxide dismutase, and catalase by shifting their acrophases to earlymiddle scotophase or amplifying the maxima. Only the inhibitory effect of melatonin on pituitary NOS-2 gene expression correlated temporally with inhibition of PRL production. Gene expression of metallothionein-1 and -3 showed maxima at earlymiddle photophase after melatonin treatment. The 24-h pattern of anterior pituitary lipid peroxidation did not vary after treatment. In vehicle-treated rats, Clock and Bmal1 expression peaked in the anterior pituitary at middle scotophase, whereas that of Per1 and Per2 and of Cry1 and Cry2 peaked at the middle and late photophase, respectively. Treatment with melatonin raised mean expression of anterior pituitary Per2, Cry1, and Cry2. In the case of Per1, decreased MESOR was observed, although the single significant difference found between the experimental groups when analyzed at individual time intervals was increase at early scotophase in the anterior pituitary of melatonin-treated rats. Melatonin significantly phase-delayed expression of Per1, Per2, and Cry1, also phase-delayed the plasma corticosterone circadian rhythm, and increased the amplitude of plasma corticosterone and thyrotropin rhythms. The results indicate that under prolonged duration of a daily melatonin signal, rat anterior pituitary PRL synthesis and release are depressed, together with significant changes in the redox and circadian mechanisms controlling them.
Fil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid; España
Fil: Barquilla, Pilar Cano. Universidad Complutense de Madrid; España
Fil: Pagano, Eleonora Samanta. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández Mateos, Pilar. Universidad Complutense de Madrid; España
Fil: Esquifino, Ana I.. Universidad Complutense de Madrid; España
Fil: Cardinali, Daniel Pedro. Universidad de Buenos Aires; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
CORTICOSTERONE
FREE RADICALS
LH
METALLOTHIONEINS
SEASONALITY
TESTOSTERONE
TSH - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/196970
Ver los metadatos del registro completo
id |
CONICETDig_c4c54ab14d83999da1a42b6628536c17 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/196970 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanismsJiménez Ortega, VanesaBarquilla, Pilar CanoPagano, Eleonora SamantaFernández Mateos, PilarEsquifino, Ana I.Cardinali, Daniel PedroCORTICOSTERONEFREE RADICALSLHMETALLOTHIONEINSSEASONALITYTESTOSTERONETSHhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In the laboratory rat, a number of physiological parameters display seasonal changes even under constant conditions of temperature, lighting, and food availability. Since there is evidence that prolactin (PRL) is, among the endocrine signals, a major mediator of seasonal adaptations, the authors aimed to examine whether melatonin administration in drinking water resembling in length the exposure to a winter photoperiod could affect accordingly the 24-h pattern of PRL synthesis and release and some of their anterior pituitary redox state and circadian clock modulatory mechanisms. Melatonin (3gmL drinking water) or vehicle was given for 1 mo, and rats were euthanized at six time intervals during a 24-h cycle. High concentrations of melatonin (>2000 pgmL) were detected in melatonin-treated rats from beginning of scotophase (at 21:00h) to early photophase (at 09:00h) as compared with a considerably narrower high-melatonin phase observed in controls. By cosinor analysis, melatonin-treated rats had significantly decreased MESOR (24-h time-series average) values of anterior pituitary PRL gene expression and circulating PRL, with acrophases (peak time) located in the middle of the scotophase, as in the control group. Melatonin treatment disrupted the 24-h pattern of anterior pituitary gene expression of nitric oxide synthase (NOS)-1 and -2, heme oxygenase-1 and -2, glutathione peroxidase, glutathione reductase, CuZn- and Mn-superoxide dismutase, and catalase by shifting their acrophases to earlymiddle scotophase or amplifying the maxima. Only the inhibitory effect of melatonin on pituitary NOS-2 gene expression correlated temporally with inhibition of PRL production. Gene expression of metallothionein-1 and -3 showed maxima at earlymiddle photophase after melatonin treatment. The 24-h pattern of anterior pituitary lipid peroxidation did not vary after treatment. In vehicle-treated rats, Clock and Bmal1 expression peaked in the anterior pituitary at middle scotophase, whereas that of Per1 and Per2 and of Cry1 and Cry2 peaked at the middle and late photophase, respectively. Treatment with melatonin raised mean expression of anterior pituitary Per2, Cry1, and Cry2. In the case of Per1, decreased MESOR was observed, although the single significant difference found between the experimental groups when analyzed at individual time intervals was increase at early scotophase in the anterior pituitary of melatonin-treated rats. Melatonin significantly phase-delayed expression of Per1, Per2, and Cry1, also phase-delayed the plasma corticosterone circadian rhythm, and increased the amplitude of plasma corticosterone and thyrotropin rhythms. The results indicate that under prolonged duration of a daily melatonin signal, rat anterior pituitary PRL synthesis and release are depressed, together with significant changes in the redox and circadian mechanisms controlling them.Fil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid; EspañaFil: Barquilla, Pilar Cano. Universidad Complutense de Madrid; EspañaFil: Pagano, Eleonora Samanta. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernández Mateos, Pilar. Universidad Complutense de Madrid; EspañaFil: Esquifino, Ana I.. Universidad Complutense de Madrid; EspañaFil: Cardinali, Daniel Pedro. Universidad de Buenos Aires; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaTaylor & Francis2012-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/196970Jiménez Ortega, Vanesa; Barquilla, Pilar Cano; Pagano, Eleonora Samanta; Fernández Mateos, Pilar; Esquifino, Ana I.; et al.; Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms; Taylor & Francis; Chronobiology International; 29; 8; 8-2012; 1021-10350742-0528CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://informahealthcare.com/doi/abs/10.3109/07420528.2012.705936info:eu-repo/semantics/altIdentifier/doi/10.3109/07420528.2012.705936info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:54Zoai:ri.conicet.gov.ar:11336/196970instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:54.515CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms |
title |
Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms |
spellingShingle |
Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms Jiménez Ortega, Vanesa CORTICOSTERONE FREE RADICALS LH METALLOTHIONEINS SEASONALITY TESTOSTERONE TSH |
title_short |
Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms |
title_full |
Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms |
title_fullStr |
Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms |
title_full_unstemmed |
Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms |
title_sort |
Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms |
dc.creator.none.fl_str_mv |
Jiménez Ortega, Vanesa Barquilla, Pilar Cano Pagano, Eleonora Samanta Fernández Mateos, Pilar Esquifino, Ana I. Cardinali, Daniel Pedro |
author |
Jiménez Ortega, Vanesa |
author_facet |
Jiménez Ortega, Vanesa Barquilla, Pilar Cano Pagano, Eleonora Samanta Fernández Mateos, Pilar Esquifino, Ana I. Cardinali, Daniel Pedro |
author_role |
author |
author2 |
Barquilla, Pilar Cano Pagano, Eleonora Samanta Fernández Mateos, Pilar Esquifino, Ana I. Cardinali, Daniel Pedro |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
CORTICOSTERONE FREE RADICALS LH METALLOTHIONEINS SEASONALITY TESTOSTERONE TSH |
topic |
CORTICOSTERONE FREE RADICALS LH METALLOTHIONEINS SEASONALITY TESTOSTERONE TSH |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
In the laboratory rat, a number of physiological parameters display seasonal changes even under constant conditions of temperature, lighting, and food availability. Since there is evidence that prolactin (PRL) is, among the endocrine signals, a major mediator of seasonal adaptations, the authors aimed to examine whether melatonin administration in drinking water resembling in length the exposure to a winter photoperiod could affect accordingly the 24-h pattern of PRL synthesis and release and some of their anterior pituitary redox state and circadian clock modulatory mechanisms. Melatonin (3gmL drinking water) or vehicle was given for 1 mo, and rats were euthanized at six time intervals during a 24-h cycle. High concentrations of melatonin (>2000 pgmL) were detected in melatonin-treated rats from beginning of scotophase (at 21:00h) to early photophase (at 09:00h) as compared with a considerably narrower high-melatonin phase observed in controls. By cosinor analysis, melatonin-treated rats had significantly decreased MESOR (24-h time-series average) values of anterior pituitary PRL gene expression and circulating PRL, with acrophases (peak time) located in the middle of the scotophase, as in the control group. Melatonin treatment disrupted the 24-h pattern of anterior pituitary gene expression of nitric oxide synthase (NOS)-1 and -2, heme oxygenase-1 and -2, glutathione peroxidase, glutathione reductase, CuZn- and Mn-superoxide dismutase, and catalase by shifting their acrophases to earlymiddle scotophase or amplifying the maxima. Only the inhibitory effect of melatonin on pituitary NOS-2 gene expression correlated temporally with inhibition of PRL production. Gene expression of metallothionein-1 and -3 showed maxima at earlymiddle photophase after melatonin treatment. The 24-h pattern of anterior pituitary lipid peroxidation did not vary after treatment. In vehicle-treated rats, Clock and Bmal1 expression peaked in the anterior pituitary at middle scotophase, whereas that of Per1 and Per2 and of Cry1 and Cry2 peaked at the middle and late photophase, respectively. Treatment with melatonin raised mean expression of anterior pituitary Per2, Cry1, and Cry2. In the case of Per1, decreased MESOR was observed, although the single significant difference found between the experimental groups when analyzed at individual time intervals was increase at early scotophase in the anterior pituitary of melatonin-treated rats. Melatonin significantly phase-delayed expression of Per1, Per2, and Cry1, also phase-delayed the plasma corticosterone circadian rhythm, and increased the amplitude of plasma corticosterone and thyrotropin rhythms. The results indicate that under prolonged duration of a daily melatonin signal, rat anterior pituitary PRL synthesis and release are depressed, together with significant changes in the redox and circadian mechanisms controlling them. Fil: Jiménez Ortega, Vanesa. Universidad Complutense de Madrid; España Fil: Barquilla, Pilar Cano. Universidad Complutense de Madrid; España Fil: Pagano, Eleonora Samanta. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fernández Mateos, Pilar. Universidad Complutense de Madrid; España Fil: Esquifino, Ana I.. Universidad Complutense de Madrid; España Fil: Cardinali, Daniel Pedro. Universidad de Buenos Aires; Argentina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
In the laboratory rat, a number of physiological parameters display seasonal changes even under constant conditions of temperature, lighting, and food availability. Since there is evidence that prolactin (PRL) is, among the endocrine signals, a major mediator of seasonal adaptations, the authors aimed to examine whether melatonin administration in drinking water resembling in length the exposure to a winter photoperiod could affect accordingly the 24-h pattern of PRL synthesis and release and some of their anterior pituitary redox state and circadian clock modulatory mechanisms. Melatonin (3gmL drinking water) or vehicle was given for 1 mo, and rats were euthanized at six time intervals during a 24-h cycle. High concentrations of melatonin (>2000 pgmL) were detected in melatonin-treated rats from beginning of scotophase (at 21:00h) to early photophase (at 09:00h) as compared with a considerably narrower high-melatonin phase observed in controls. By cosinor analysis, melatonin-treated rats had significantly decreased MESOR (24-h time-series average) values of anterior pituitary PRL gene expression and circulating PRL, with acrophases (peak time) located in the middle of the scotophase, as in the control group. Melatonin treatment disrupted the 24-h pattern of anterior pituitary gene expression of nitric oxide synthase (NOS)-1 and -2, heme oxygenase-1 and -2, glutathione peroxidase, glutathione reductase, CuZn- and Mn-superoxide dismutase, and catalase by shifting their acrophases to earlymiddle scotophase or amplifying the maxima. Only the inhibitory effect of melatonin on pituitary NOS-2 gene expression correlated temporally with inhibition of PRL production. Gene expression of metallothionein-1 and -3 showed maxima at earlymiddle photophase after melatonin treatment. The 24-h pattern of anterior pituitary lipid peroxidation did not vary after treatment. In vehicle-treated rats, Clock and Bmal1 expression peaked in the anterior pituitary at middle scotophase, whereas that of Per1 and Per2 and of Cry1 and Cry2 peaked at the middle and late photophase, respectively. Treatment with melatonin raised mean expression of anterior pituitary Per2, Cry1, and Cry2. In the case of Per1, decreased MESOR was observed, although the single significant difference found between the experimental groups when analyzed at individual time intervals was increase at early scotophase in the anterior pituitary of melatonin-treated rats. Melatonin significantly phase-delayed expression of Per1, Per2, and Cry1, also phase-delayed the plasma corticosterone circadian rhythm, and increased the amplitude of plasma corticosterone and thyrotropin rhythms. The results indicate that under prolonged duration of a daily melatonin signal, rat anterior pituitary PRL synthesis and release are depressed, together with significant changes in the redox and circadian mechanisms controlling them. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/196970 Jiménez Ortega, Vanesa; Barquilla, Pilar Cano; Pagano, Eleonora Samanta; Fernández Mateos, Pilar; Esquifino, Ana I.; et al.; Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms; Taylor & Francis; Chronobiology International; 29; 8; 8-2012; 1021-1035 0742-0528 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/196970 |
identifier_str_mv |
Jiménez Ortega, Vanesa; Barquilla, Pilar Cano; Pagano, Eleonora Samanta; Fernández Mateos, Pilar; Esquifino, Ana I.; et al.; Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis: Correlation with anterior pituitary redox state and circadian clock mechanisms; Taylor & Francis; Chronobiology International; 29; 8; 8-2012; 1021-1035 0742-0528 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://informahealthcare.com/doi/abs/10.3109/07420528.2012.705936 info:eu-repo/semantics/altIdentifier/doi/10.3109/07420528.2012.705936 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Taylor & Francis |
publisher.none.fl_str_mv |
Taylor & Francis |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614524076818432 |
score |
13.070432 |