Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin
- Autores
- Jiménez Ortega, Vanesa; Cano Barquilla, Pilar; Fernández Mateos, María P.; Cardinali, Daniel Pedro; Esquifino, Ana I.
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión enviada
- Descripción
- Fil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Cano Barquilla, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Fernández Mateos, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Fernández Mateos, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Biología Celular; España
Fil: Cardinali, Daniel P. Pontificia Universidad Católica Argentina. Facultad de Medicina. Departamento de Docencia e Investigación; Argentina
Fil: Cardinali, Daniel P. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología; Argentina
Fil: Esquifino, Ana I. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Abstract: To examine the effect a low dose of Cd as an endocrine disruptor, male Wistar rats received CdCl2 (5 ppm Cd) in drinking water or drinking water alone. After 1 month, rats were euthanized at one of six time intervals around the clock and the 24-h pattern of adenohypophysial PRL synthesis and release, lipid peroxidation and redox enzyme and metallothionein (MT) gene expression was examined. Cd suppressed 24-h rhythmicity in expression of PRL gene and in circulating PRL by increasing them at early photophase only, in correlation with an augmented pituitary lipid peroxidation and redox enzyme expression. CdCl2 treatment effectively disrupted the 24-h variation in expression of every pituitary parameter tested except for MT-3. In a second experiment the effect of melatonin (3 μg/mL drinking water) was assessed at early photophase, the time of maximal endocrine disrupting effect of Cd. Melatonin treatment blunted the effect of Cd on PRL synthesis and release, decreased Cd-induced lipid peroxidation and counteracted the effect of Cd on expression of most redox enzymes. A third experiment was performed to examine whether melatonin could counteract Cd-induced changes in the 24-h pattern of pituitary circadian clock gene expression and plasma PRL, LH, TSH and corticosterone levels. Rats receiving CdCl2 exhibited a suppressed daily rhythm of Clock expression and a significant disruption in daily rhythms of pituitary Bmal1, Per1, Per2, Cry1 and Cry2. The co-administration of melatonin restored rhythmicity in Clock and Bmal1 expression but shifted the maxima in pituitary Per1, Cry1 and Cry2 expression to the scotophase. Melatonin also counteracted the effect of Cd on 24-h rhythmicity of circulating PRL, LH, TSH and corticosterone. The results underline the occurrence of a significant endocrine disruptor effect of a low dose of Cd. Generally melatonin counteracted the effects of Cd and ameliorated partly the circadian disruption caused by the pollutant. - Fuente
- Free radical biology and medicine. 2012, 53
- Materia
-
CADMIO
PROLACTINA
RITMO CARDIACO
MELATONINA
EXPRESION GENICA
CORTICOSTERONA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Pontificia Universidad Católica Argentina
- OAI Identificador
- oai:ucacris:123456789/1613
Ver los metadatos del registro completo
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oai:ucacris:123456789/1613 |
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spelling |
Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatoninJiménez Ortega, VanesaCano Barquilla, PilarFernández Mateos, María P.Cardinali, Daniel PedroEsquifino, Ana I.CADMIOPROLACTINARITMO CARDIACOMELATONINAEXPRESION GENICACORTICOSTERONAFil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; EspañaFil: Cano Barquilla, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; EspañaFil: Fernández Mateos, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; EspañaFil: Fernández Mateos, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Biología Celular; EspañaFil: Cardinali, Daniel P. Pontificia Universidad Católica Argentina. Facultad de Medicina. Departamento de Docencia e Investigación; ArgentinaFil: Cardinali, Daniel P. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología; ArgentinaFil: Esquifino, Ana I. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; EspañaAbstract: To examine the effect a low dose of Cd as an endocrine disruptor, male Wistar rats received CdCl2 (5 ppm Cd) in drinking water or drinking water alone. After 1 month, rats were euthanized at one of six time intervals around the clock and the 24-h pattern of adenohypophysial PRL synthesis and release, lipid peroxidation and redox enzyme and metallothionein (MT) gene expression was examined. Cd suppressed 24-h rhythmicity in expression of PRL gene and in circulating PRL by increasing them at early photophase only, in correlation with an augmented pituitary lipid peroxidation and redox enzyme expression. CdCl2 treatment effectively disrupted the 24-h variation in expression of every pituitary parameter tested except for MT-3. In a second experiment the effect of melatonin (3 μg/mL drinking water) was assessed at early photophase, the time of maximal endocrine disrupting effect of Cd. Melatonin treatment blunted the effect of Cd on PRL synthesis and release, decreased Cd-induced lipid peroxidation and counteracted the effect of Cd on expression of most redox enzymes. A third experiment was performed to examine whether melatonin could counteract Cd-induced changes in the 24-h pattern of pituitary circadian clock gene expression and plasma PRL, LH, TSH and corticosterone levels. Rats receiving CdCl2 exhibited a suppressed daily rhythm of Clock expression and a significant disruption in daily rhythms of pituitary Bmal1, Per1, Per2, Cry1 and Cry2. The co-administration of melatonin restored rhythmicity in Clock and Bmal1 expression but shifted the maxima in pituitary Per1, Cry1 and Cry2 expression to the scotophase. Melatonin also counteracted the effect of Cd on 24-h rhythmicity of circulating PRL, LH, TSH and corticosterone. The results underline the occurrence of a significant endocrine disruptor effect of a low dose of Cd. Generally melatonin counteracted the effects of Cd and ameliorated partly the circadian disruption caused by the pollutant.Elsevier2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/16130891-584910.1016/j.freeradbiomed.2012.10.533Jiménez Ortega, V, et al. Cadmium as an endocrine disruptor. Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin [en línea]. Preprint del artículo publicado en Free radical biology and medicine. 2012, 53. doi:10.1016/j.freeradbiomed.2012.10.533. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1613Free radical biology and medicine. 2012, 53reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaengenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:55:19Zoai:ucacris:123456789/1613instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:55:19.973Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse |
dc.title.none.fl_str_mv |
Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin |
title |
Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin |
spellingShingle |
Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin Jiménez Ortega, Vanesa CADMIO PROLACTINA RITMO CARDIACO MELATONINA EXPRESION GENICA CORTICOSTERONA |
title_short |
Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin |
title_full |
Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin |
title_fullStr |
Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin |
title_full_unstemmed |
Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin |
title_sort |
Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin |
dc.creator.none.fl_str_mv |
Jiménez Ortega, Vanesa Cano Barquilla, Pilar Fernández Mateos, María P. Cardinali, Daniel Pedro Esquifino, Ana I. |
author |
Jiménez Ortega, Vanesa |
author_facet |
Jiménez Ortega, Vanesa Cano Barquilla, Pilar Fernández Mateos, María P. Cardinali, Daniel Pedro Esquifino, Ana I. |
author_role |
author |
author2 |
Cano Barquilla, Pilar Fernández Mateos, María P. Cardinali, Daniel Pedro Esquifino, Ana I. |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
CADMIO PROLACTINA RITMO CARDIACO MELATONINA EXPRESION GENICA CORTICOSTERONA |
topic |
CADMIO PROLACTINA RITMO CARDIACO MELATONINA EXPRESION GENICA CORTICOSTERONA |
dc.description.none.fl_txt_mv |
Fil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España Fil: Cano Barquilla, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España Fil: Fernández Mateos, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España Fil: Fernández Mateos, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Biología Celular; España Fil: Cardinali, Daniel P. Pontificia Universidad Católica Argentina. Facultad de Medicina. Departamento de Docencia e Investigación; Argentina Fil: Cardinali, Daniel P. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología; Argentina Fil: Esquifino, Ana I. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España Abstract: To examine the effect a low dose of Cd as an endocrine disruptor, male Wistar rats received CdCl2 (5 ppm Cd) in drinking water or drinking water alone. After 1 month, rats were euthanized at one of six time intervals around the clock and the 24-h pattern of adenohypophysial PRL synthesis and release, lipid peroxidation and redox enzyme and metallothionein (MT) gene expression was examined. Cd suppressed 24-h rhythmicity in expression of PRL gene and in circulating PRL by increasing them at early photophase only, in correlation with an augmented pituitary lipid peroxidation and redox enzyme expression. CdCl2 treatment effectively disrupted the 24-h variation in expression of every pituitary parameter tested except for MT-3. In a second experiment the effect of melatonin (3 μg/mL drinking water) was assessed at early photophase, the time of maximal endocrine disrupting effect of Cd. Melatonin treatment blunted the effect of Cd on PRL synthesis and release, decreased Cd-induced lipid peroxidation and counteracted the effect of Cd on expression of most redox enzymes. A third experiment was performed to examine whether melatonin could counteract Cd-induced changes in the 24-h pattern of pituitary circadian clock gene expression and plasma PRL, LH, TSH and corticosterone levels. Rats receiving CdCl2 exhibited a suppressed daily rhythm of Clock expression and a significant disruption in daily rhythms of pituitary Bmal1, Per1, Per2, Cry1 and Cry2. The co-administration of melatonin restored rhythmicity in Clock and Bmal1 expression but shifted the maxima in pituitary Per1, Cry1 and Cry2 expression to the scotophase. Melatonin also counteracted the effect of Cd on 24-h rhythmicity of circulating PRL, LH, TSH and corticosterone. The results underline the occurrence of a significant endocrine disruptor effect of a low dose of Cd. Generally melatonin counteracted the effects of Cd and ameliorated partly the circadian disruption caused by the pollutant. |
description |
Fil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
submittedVersion |
dc.identifier.none.fl_str_mv |
https://repositorio.uca.edu.ar/handle/123456789/1613 0891-5849 10.1016/j.freeradbiomed.2012.10.533 Jiménez Ortega, V, et al. Cadmium as an endocrine disruptor. Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin [en línea]. Preprint del artículo publicado en Free radical biology and medicine. 2012, 53. doi:10.1016/j.freeradbiomed.2012.10.533. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1613 |
url |
https://repositorio.uca.edu.ar/handle/123456789/1613 |
identifier_str_mv |
0891-5849 10.1016/j.freeradbiomed.2012.10.533 Jiménez Ortega, V, et al. Cadmium as an endocrine disruptor. Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin [en línea]. Preprint del artículo publicado en Free radical biology and medicine. 2012, 53. doi:10.1016/j.freeradbiomed.2012.10.533. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1613 |
dc.language.none.fl_str_mv |
eng eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
Free radical biology and medicine. 2012, 53 reponame:Repositorio Institucional (UCA) instname:Pontificia Universidad Católica Argentina |
reponame_str |
Repositorio Institucional (UCA) |
collection |
Repositorio Institucional (UCA) |
instname_str |
Pontificia Universidad Católica Argentina |
repository.name.fl_str_mv |
Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina |
repository.mail.fl_str_mv |
claudia_fernandez@uca.edu.ar |
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1836638330543931392 |
score |
13.22299 |