Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin

Autores
Jiménez Ortega, Vanesa; Cano Barquilla, Pilar; Fernández Mateos, María P.; Cardinali, Daniel Pedro; Esquifino, Ana I.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
Fil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Cano Barquilla, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Fernández Mateos, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Fernández Mateos, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Biología Celular; España
Fil: Cardinali, Daniel P. Pontificia Universidad Católica Argentina. Facultad de Medicina. Departamento de Docencia e Investigación; Argentina
Fil: Cardinali, Daniel P. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología; Argentina
Fil: Esquifino, Ana I. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Abstract: To examine the effect a low dose of Cd as an endocrine disruptor, male Wistar rats received CdCl2 (5 ppm Cd) in drinking water or drinking water alone. After 1 month, rats were euthanized at one of six time intervals around the clock and the 24-h pattern of adenohypophysial PRL synthesis and release, lipid peroxidation and redox enzyme and metallothionein (MT) gene expression was examined. Cd suppressed 24-h rhythmicity in expression of PRL gene and in circulating PRL by increasing them at early photophase only, in correlation with an augmented pituitary lipid peroxidation and redox enzyme expression. CdCl2 treatment effectively disrupted the 24-h variation in expression of every pituitary parameter tested except for MT-3. In a second experiment the effect of melatonin (3 μg/mL drinking water) was assessed at early photophase, the time of maximal endocrine disrupting effect of Cd. Melatonin treatment blunted the effect of Cd on PRL synthesis and release, decreased Cd-induced lipid peroxidation and counteracted the effect of Cd on expression of most redox enzymes. A third experiment was performed to examine whether melatonin could counteract Cd-induced changes in the 24-h pattern of pituitary circadian clock gene expression and plasma PRL, LH, TSH and corticosterone levels. Rats receiving CdCl2 exhibited a suppressed daily rhythm of Clock expression and a significant disruption in daily rhythms of pituitary Bmal1, Per1, Per2, Cry1 and Cry2. The co-administration of melatonin restored rhythmicity in Clock and Bmal1 expression but shifted the maxima in pituitary Per1, Cry1 and Cry2 expression to the scotophase. Melatonin also counteracted the effect of Cd on 24-h rhythmicity of circulating PRL, LH, TSH and corticosterone. The results underline the occurrence of a significant endocrine disruptor effect of a low dose of Cd. Generally melatonin counteracted the effects of Cd and ameliorated partly the circadian disruption caused by the pollutant.
Fuente
Free radical biology and medicine. 2012, 53
Materia
CADMIO
PROLACTINA
RITMO CARDIACO
MELATONINA
EXPRESION GENICA
CORTICOSTERONA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/1613

id RIUCA_73e5e6a8951d41d1e2e006a668686447
oai_identifier_str oai:ucacris:123456789/1613
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatoninJiménez Ortega, VanesaCano Barquilla, PilarFernández Mateos, María P.Cardinali, Daniel PedroEsquifino, Ana I.CADMIOPROLACTINARITMO CARDIACOMELATONINAEXPRESION GENICACORTICOSTERONAFil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; EspañaFil: Cano Barquilla, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; EspañaFil: Fernández Mateos, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; EspañaFil: Fernández Mateos, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Biología Celular; EspañaFil: Cardinali, Daniel P. Pontificia Universidad Católica Argentina. Facultad de Medicina. Departamento de Docencia e Investigación; ArgentinaFil: Cardinali, Daniel P. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología; ArgentinaFil: Esquifino, Ana I. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; EspañaAbstract: To examine the effect a low dose of Cd as an endocrine disruptor, male Wistar rats received CdCl2 (5 ppm Cd) in drinking water or drinking water alone. After 1 month, rats were euthanized at one of six time intervals around the clock and the 24-h pattern of adenohypophysial PRL synthesis and release, lipid peroxidation and redox enzyme and metallothionein (MT) gene expression was examined. Cd suppressed 24-h rhythmicity in expression of PRL gene and in circulating PRL by increasing them at early photophase only, in correlation with an augmented pituitary lipid peroxidation and redox enzyme expression. CdCl2 treatment effectively disrupted the 24-h variation in expression of every pituitary parameter tested except for MT-3. In a second experiment the effect of melatonin (3 μg/mL drinking water) was assessed at early photophase, the time of maximal endocrine disrupting effect of Cd. Melatonin treatment blunted the effect of Cd on PRL synthesis and release, decreased Cd-induced lipid peroxidation and counteracted the effect of Cd on expression of most redox enzymes. A third experiment was performed to examine whether melatonin could counteract Cd-induced changes in the 24-h pattern of pituitary circadian clock gene expression and plasma PRL, LH, TSH and corticosterone levels. Rats receiving CdCl2 exhibited a suppressed daily rhythm of Clock expression and a significant disruption in daily rhythms of pituitary Bmal1, Per1, Per2, Cry1 and Cry2. The co-administration of melatonin restored rhythmicity in Clock and Bmal1 expression but shifted the maxima in pituitary Per1, Cry1 and Cry2 expression to the scotophase. Melatonin also counteracted the effect of Cd on 24-h rhythmicity of circulating PRL, LH, TSH and corticosterone. The results underline the occurrence of a significant endocrine disruptor effect of a low dose of Cd. Generally melatonin counteracted the effects of Cd and ameliorated partly the circadian disruption caused by the pollutant.Elsevier2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/16130891-584910.1016/j.freeradbiomed.2012.10.533Jiménez Ortega, V, et al. Cadmium as an endocrine disruptor. Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin [en línea]. Preprint del artículo publicado en Free radical biology and medicine. 2012, 53. doi:10.1016/j.freeradbiomed.2012.10.533. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1613Free radical biology and medicine. 2012, 53reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaengenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:55:19Zoai:ucacris:123456789/1613instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:55:19.973Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin
title Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin
spellingShingle Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin
Jiménez Ortega, Vanesa
CADMIO
PROLACTINA
RITMO CARDIACO
MELATONINA
EXPRESION GENICA
CORTICOSTERONA
title_short Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin
title_full Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin
title_fullStr Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin
title_full_unstemmed Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin
title_sort Cadmium as an endocrine disruptor : correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin
dc.creator.none.fl_str_mv Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Fernández Mateos, María P.
Cardinali, Daniel Pedro
Esquifino, Ana I.
author Jiménez Ortega, Vanesa
author_facet Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Fernández Mateos, María P.
Cardinali, Daniel Pedro
Esquifino, Ana I.
author_role author
author2 Cano Barquilla, Pilar
Fernández Mateos, María P.
Cardinali, Daniel Pedro
Esquifino, Ana I.
author2_role author
author
author
author
dc.subject.none.fl_str_mv CADMIO
PROLACTINA
RITMO CARDIACO
MELATONINA
EXPRESION GENICA
CORTICOSTERONA
topic CADMIO
PROLACTINA
RITMO CARDIACO
MELATONINA
EXPRESION GENICA
CORTICOSTERONA
dc.description.none.fl_txt_mv Fil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Cano Barquilla, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Fernández Mateos, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Fernández Mateos, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Biología Celular; España
Fil: Cardinali, Daniel P. Pontificia Universidad Católica Argentina. Facultad de Medicina. Departamento de Docencia e Investigación; Argentina
Fil: Cardinali, Daniel P. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología; Argentina
Fil: Esquifino, Ana I. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Abstract: To examine the effect a low dose of Cd as an endocrine disruptor, male Wistar rats received CdCl2 (5 ppm Cd) in drinking water or drinking water alone. After 1 month, rats were euthanized at one of six time intervals around the clock and the 24-h pattern of adenohypophysial PRL synthesis and release, lipid peroxidation and redox enzyme and metallothionein (MT) gene expression was examined. Cd suppressed 24-h rhythmicity in expression of PRL gene and in circulating PRL by increasing them at early photophase only, in correlation with an augmented pituitary lipid peroxidation and redox enzyme expression. CdCl2 treatment effectively disrupted the 24-h variation in expression of every pituitary parameter tested except for MT-3. In a second experiment the effect of melatonin (3 μg/mL drinking water) was assessed at early photophase, the time of maximal endocrine disrupting effect of Cd. Melatonin treatment blunted the effect of Cd on PRL synthesis and release, decreased Cd-induced lipid peroxidation and counteracted the effect of Cd on expression of most redox enzymes. A third experiment was performed to examine whether melatonin could counteract Cd-induced changes in the 24-h pattern of pituitary circadian clock gene expression and plasma PRL, LH, TSH and corticosterone levels. Rats receiving CdCl2 exhibited a suppressed daily rhythm of Clock expression and a significant disruption in daily rhythms of pituitary Bmal1, Per1, Per2, Cry1 and Cry2. The co-administration of melatonin restored rhythmicity in Clock and Bmal1 expression but shifted the maxima in pituitary Per1, Cry1 and Cry2 expression to the scotophase. Melatonin also counteracted the effect of Cd on 24-h rhythmicity of circulating PRL, LH, TSH and corticosterone. The results underline the occurrence of a significant endocrine disruptor effect of a low dose of Cd. Generally melatonin counteracted the effects of Cd and ameliorated partly the circadian disruption caused by the pollutant.
description Fil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/1613
0891-5849
10.1016/j.freeradbiomed.2012.10.533
Jiménez Ortega, V, et al. Cadmium as an endocrine disruptor. Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin [en línea]. Preprint del artículo publicado en Free radical biology and medicine. 2012, 53. doi:10.1016/j.freeradbiomed.2012.10.533. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1613
url https://repositorio.uca.edu.ar/handle/123456789/1613
identifier_str_mv 0891-5849
10.1016/j.freeradbiomed.2012.10.533
Jiménez Ortega, V, et al. Cadmium as an endocrine disruptor. Correlation with anterior pituitary redox and circadian clock mechanisms and prevention by melatonin [en línea]. Preprint del artículo publicado en Free radical biology and medicine. 2012, 53. doi:10.1016/j.freeradbiomed.2012.10.533. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1613
dc.language.none.fl_str_mv eng
eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Free radical biology and medicine. 2012, 53
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.22299