Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis : correlation with anterior pituitary redox state and circadian clock mechanisms

Autores
Jiménez Ortega, Vanesa; Cano Barquilla, Pilar; Pagano, Eleonora Samanta; Fernández Mateos, Ana; Esquifino, Ana I.; Cardinali, Daniel Pedro
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
Fil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Cano Barquilla, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Pagano, Eleonora S. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Departamento de Docencia e Investigación; Argentina
Fil: Fernández Mateos, Ana. Universidad Complutense. Facultad de Medicina. Departamento de Biología Celuar; España
Fil: Esquifino, Ana I. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Cardinali, Daniel P. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Departamento de Docencia e Investigación; Argentina
Fil: Cardinali, Daniel P. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología; Argentina
Abstract: In the laboratory rat a number of physiological parameters display seasonal changes even under constant conditions of temperature, lighting and food availability. Since there is evidence that prolactin (PRL) is, among the endocrine signals, a major mediator of seasonal adaptations, we aimed to examine whether melatonin administration in drinking water resembling in length the exposure to a winter photoperiod could affect accordingly the 24-h pattern of PRL synthesis and release and some of their pituitary redox state- and circadian clock modulatory mechanisms. Melatonin (3 μg/mL drinking water) or vehicle was given for a month and rats were eutanized at 6 time intervals during a 24-h cycle. High concentrations of melatonin (more than 2000 pg/mL) were detected in melatonin-treated rats from the beginning of scotophase (at 21:00 h) to early photophase (at 09:00 h) as compared to a considerably narrower high melatonin phase observed in controls. In a cosinor analysis, melatonin-treated rats had significantly decreased mesor values of pituitary PRL gene expression and circulating PRL levels with acrophases at middle of scotophase as in the control group. Melatonin treatment disrupted the 24-h pattern in anterior pituitary gene expression of nitric oxide synthase (NOS)-1 and -2, heme oxygenase-1 and -2, glutathione peroxidase, glutathione reductase, Cu/Zn- and Mnsuperoxide dismutases and catalase by shifting their acrophases to early/middle scotophase or by amplifying the maxima found. Only the inhibitory effect of melatonin on pituitary NOS-2 gene expression correlated temporally with the inhibition of PRL production. Gene expression of metallothionein-1 and -3 showed maxima at early/middle photophase after melatonin treatment. 24-Hour pattern of pituitary lipid peroxidation did not vary after treatment. In vehicle-treated rats, Clock and Bmal1 expression peaked at mid scotophase while that of Per1 and Per2, and of Cry1 and Cry2, peaked at mid and late photophase, respectively. Treatment with melatonin decreased mean expression of Per1 and raised that of Per2, Cry1 and Cry2. Melatonin significantly phase-delayed expression of Per1, Per2 and Cry1. Melatonin also phasedelayed plasma corticosterone rhythm and increased the amplitude of plasma corticosterone and TSH rhythms. The results indicate that under a prolonged duration of a daily melatonin signal, rat pituitary PRL synthesis and release are depressed together with significant changes in the redox and circadian mechanisms controlling them
Fuente
Preprint del documento publicado en Chronobiology international, 2012
Materia
MELATONINA
ESTACIONALIDAD
CORTICOSTERONA
TETOSTERONA
METALOTIONEINA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/1628

id RIUCA_e05e3a11f80b257c9f63a16d7b893aec
oai_identifier_str oai:ucacris:123456789/1628
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis : correlation with anterior pituitary redox state and circadian clock mechanismsJiménez Ortega, VanesaCano Barquilla, PilarPagano, Eleonora SamantaFernández Mateos, AnaEsquifino, Ana I.Cardinali, Daniel PedroMELATONINAESTACIONALIDADCORTICOSTERONATETOSTERONAMETALOTIONEINAFil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; EspañaFil: Cano Barquilla, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; EspañaFil: Pagano, Eleonora S. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Departamento de Docencia e Investigación; ArgentinaFil: Fernández Mateos, Ana. Universidad Complutense. Facultad de Medicina. Departamento de Biología Celuar; EspañaFil: Esquifino, Ana I. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; EspañaFil: Cardinali, Daniel P. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Departamento de Docencia e Investigación; ArgentinaFil: Cardinali, Daniel P. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología; ArgentinaAbstract: In the laboratory rat a number of physiological parameters display seasonal changes even under constant conditions of temperature, lighting and food availability. Since there is evidence that prolactin (PRL) is, among the endocrine signals, a major mediator of seasonal adaptations, we aimed to examine whether melatonin administration in drinking water resembling in length the exposure to a winter photoperiod could affect accordingly the 24-h pattern of PRL synthesis and release and some of their pituitary redox state- and circadian clock modulatory mechanisms. Melatonin (3 μg/mL drinking water) or vehicle was given for a month and rats were eutanized at 6 time intervals during a 24-h cycle. High concentrations of melatonin (more than 2000 pg/mL) were detected in melatonin-treated rats from the beginning of scotophase (at 21:00 h) to early photophase (at 09:00 h) as compared to a considerably narrower high melatonin phase observed in controls. In a cosinor analysis, melatonin-treated rats had significantly decreased mesor values of pituitary PRL gene expression and circulating PRL levels with acrophases at middle of scotophase as in the control group. Melatonin treatment disrupted the 24-h pattern in anterior pituitary gene expression of nitric oxide synthase (NOS)-1 and -2, heme oxygenase-1 and -2, glutathione peroxidase, glutathione reductase, Cu/Zn- and Mnsuperoxide dismutases and catalase by shifting their acrophases to early/middle scotophase or by amplifying the maxima found. Only the inhibitory effect of melatonin on pituitary NOS-2 gene expression correlated temporally with the inhibition of PRL production. Gene expression of metallothionein-1 and -3 showed maxima at early/middle photophase after melatonin treatment. 24-Hour pattern of pituitary lipid peroxidation did not vary after treatment. In vehicle-treated rats, Clock and Bmal1 expression peaked at mid scotophase while that of Per1 and Per2, and of Cry1 and Cry2, peaked at mid and late photophase, respectively. Treatment with melatonin decreased mean expression of Per1 and raised that of Per2, Cry1 and Cry2. Melatonin significantly phase-delayed expression of Per1, Per2 and Cry1. Melatonin also phasedelayed plasma corticosterone rhythm and increased the amplitude of plasma corticosterone and TSH rhythms. The results indicate that under a prolonged duration of a daily melatonin signal, rat pituitary PRL synthesis and release are depressed together with significant changes in the redox and circadian mechanisms controlling them2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/16280742-0528 (impreso)1525-6073 (online)10.3109/07420528.2012.705936Jiménez Ortega, V., et al. Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis : correlation with anterior pituitary redox state and circadian clock mechanisms [en línea]. Preprint del documento publicado en Chronobiology international, 2012. doi:10.3109/07420528.2012.705936. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1628Preprint del documento publicado en Chronobiology international, 2012reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaengenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:55:19Zoai:ucacris:123456789/1628instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:55:20.014Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis : correlation with anterior pituitary redox state and circadian clock mechanisms
title Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis : correlation with anterior pituitary redox state and circadian clock mechanisms
spellingShingle Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis : correlation with anterior pituitary redox state and circadian clock mechanisms
Jiménez Ortega, Vanesa
MELATONINA
ESTACIONALIDAD
CORTICOSTERONA
TETOSTERONA
METALOTIONEINA
title_short Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis : correlation with anterior pituitary redox state and circadian clock mechanisms
title_full Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis : correlation with anterior pituitary redox state and circadian clock mechanisms
title_fullStr Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis : correlation with anterior pituitary redox state and circadian clock mechanisms
title_full_unstemmed Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis : correlation with anterior pituitary redox state and circadian clock mechanisms
title_sort Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis : correlation with anterior pituitary redox state and circadian clock mechanisms
dc.creator.none.fl_str_mv Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Pagano, Eleonora Samanta
Fernández Mateos, Ana
Esquifino, Ana I.
Cardinali, Daniel Pedro
author Jiménez Ortega, Vanesa
author_facet Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Pagano, Eleonora Samanta
Fernández Mateos, Ana
Esquifino, Ana I.
Cardinali, Daniel Pedro
author_role author
author2 Cano Barquilla, Pilar
Pagano, Eleonora Samanta
Fernández Mateos, Ana
Esquifino, Ana I.
Cardinali, Daniel Pedro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv MELATONINA
ESTACIONALIDAD
CORTICOSTERONA
TETOSTERONA
METALOTIONEINA
topic MELATONINA
ESTACIONALIDAD
CORTICOSTERONA
TETOSTERONA
METALOTIONEINA
dc.description.none.fl_txt_mv Fil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Cano Barquilla, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Pagano, Eleonora S. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Departamento de Docencia e Investigación; Argentina
Fil: Fernández Mateos, Ana. Universidad Complutense. Facultad de Medicina. Departamento de Biología Celuar; España
Fil: Esquifino, Ana I. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Cardinali, Daniel P. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Departamento de Docencia e Investigación; Argentina
Fil: Cardinali, Daniel P. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología; Argentina
Abstract: In the laboratory rat a number of physiological parameters display seasonal changes even under constant conditions of temperature, lighting and food availability. Since there is evidence that prolactin (PRL) is, among the endocrine signals, a major mediator of seasonal adaptations, we aimed to examine whether melatonin administration in drinking water resembling in length the exposure to a winter photoperiod could affect accordingly the 24-h pattern of PRL synthesis and release and some of their pituitary redox state- and circadian clock modulatory mechanisms. Melatonin (3 μg/mL drinking water) or vehicle was given for a month and rats were eutanized at 6 time intervals during a 24-h cycle. High concentrations of melatonin (more than 2000 pg/mL) were detected in melatonin-treated rats from the beginning of scotophase (at 21:00 h) to early photophase (at 09:00 h) as compared to a considerably narrower high melatonin phase observed in controls. In a cosinor analysis, melatonin-treated rats had significantly decreased mesor values of pituitary PRL gene expression and circulating PRL levels with acrophases at middle of scotophase as in the control group. Melatonin treatment disrupted the 24-h pattern in anterior pituitary gene expression of nitric oxide synthase (NOS)-1 and -2, heme oxygenase-1 and -2, glutathione peroxidase, glutathione reductase, Cu/Zn- and Mnsuperoxide dismutases and catalase by shifting their acrophases to early/middle scotophase or by amplifying the maxima found. Only the inhibitory effect of melatonin on pituitary NOS-2 gene expression correlated temporally with the inhibition of PRL production. Gene expression of metallothionein-1 and -3 showed maxima at early/middle photophase after melatonin treatment. 24-Hour pattern of pituitary lipid peroxidation did not vary after treatment. In vehicle-treated rats, Clock and Bmal1 expression peaked at mid scotophase while that of Per1 and Per2, and of Cry1 and Cry2, peaked at mid and late photophase, respectively. Treatment with melatonin decreased mean expression of Per1 and raised that of Per2, Cry1 and Cry2. Melatonin significantly phase-delayed expression of Per1, Per2 and Cry1. Melatonin also phasedelayed plasma corticosterone rhythm and increased the amplitude of plasma corticosterone and TSH rhythms. The results indicate that under a prolonged duration of a daily melatonin signal, rat pituitary PRL synthesis and release are depressed together with significant changes in the redox and circadian mechanisms controlling them
description Fil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/1628
0742-0528 (impreso)
1525-6073 (online)
10.3109/07420528.2012.705936
Jiménez Ortega, V., et al. Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis : correlation with anterior pituitary redox state and circadian clock mechanisms [en línea]. Preprint del documento publicado en Chronobiology international, 2012. doi:10.3109/07420528.2012.705936. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1628
url https://repositorio.uca.edu.ar/handle/123456789/1628
identifier_str_mv 0742-0528 (impreso)
1525-6073 (online)
10.3109/07420528.2012.705936
Jiménez Ortega, V., et al. Melatonin supplementation decreases prolactin synthesis and release in rat adenohypophysis : correlation with anterior pituitary redox state and circadian clock mechanisms [en línea]. Preprint del documento publicado en Chronobiology international, 2012. doi:10.3109/07420528.2012.705936. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1628
dc.language.none.fl_str_mv eng
eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Preprint del documento publicado en Chronobiology international, 2012
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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