Oxidative stress response in reference and clinical Staphylococcus aureus strains under Linezolid exposure

Autores
Martinez, Sol Romina; Aiassa, Virginia; Sola, Claudia del Valle; Becerra, María Cecilia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) strains are some of the most widespread pathogens multi-resistant to antimicrobial agents (AA). The AA provoke several changes inside bacteria, which cannot be solely explained by the main mechanisms of action reported. OBJECTIVE:The role of the oxidative stress in bacteria exposed to bacteriostatic AA has not been widely studied; hence, the aim of our work was to investigate the effect of Linezolid (LZD) on S. aureus strains. METHODS:Oxidative stress markers, such as superoxide dismutase enzyme (SOD) activity, the global antioxidant response, the advanced oxidation protein products (AOPP) and the basal levels of glutathione in 28 clinical and 2 reference strains were performed. RESULTS AND CONCLUSIONS:We identified 10 of 30 strains showing a slightly increase in reactive species under LZD treatment with respect to the untreated control (between 22 to 56%). The higher generation was detected in the clinical strains compared to the reference ones, however the impact in the antioxidant response was not significant, and the oxidized protein levels were almost undetectable. The strains exposed to this oxazolidinone did not suffer acute oxidative stress. This is the first work reporting the behavior of S. aureus clinical and reference strains exposed to LZD, being the oxidative stress negligible.
Fil: Martinez, Sol Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Aiassa, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Sola, Claudia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Materia
STAPHYLOCOCCUS AUREUS
OXIDATIVE STRESS
OXAZOLIDINONE
ANTIMICROBIAL RESISTANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/111844
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/111844
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Oxidative stress response in reference and clinical Staphylococcus aureus strains under Linezolid exposureMartinez, Sol RominaAiassa, VirginiaSola, Claudia del ValleBecerra, María CeciliaSTAPHYLOCOCCUS AUREUSOXIDATIVE STRESSOXAZOLIDINONEANTIMICROBIAL RESISTANCEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) strains are some of the most widespread pathogens multi-resistant to antimicrobial agents (AA). The AA provoke several changes inside bacteria, which cannot be solely explained by the main mechanisms of action reported. OBJECTIVE:The role of the oxidative stress in bacteria exposed to bacteriostatic AA has not been widely studied; hence, the aim of our work was to investigate the effect of Linezolid (LZD) on S. aureus strains. METHODS:Oxidative stress markers, such as superoxide dismutase enzyme (SOD) activity, the global antioxidant response, the advanced oxidation protein products (AOPP) and the basal levels of glutathione in 28 clinical and 2 reference strains were performed. RESULTS AND CONCLUSIONS:We identified 10 of 30 strains showing a slightly increase in reactive species under LZD treatment with respect to the untreated control (between 22 to 56%). The higher generation was detected in the clinical strains compared to the reference ones, however the impact in the antioxidant response was not significant, and the oxidized protein levels were almost undetectable. The strains exposed to this oxazolidinone did not suffer acute oxidative stress. This is the first work reporting the behavior of S. aureus clinical and reference strains exposed to LZD, being the oxidative stress negligible.Fil: Martinez, Sol Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Aiassa, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; ArgentinaFil: Sola, Claudia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaElsevier2020-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/111844Martinez, Sol Romina; Aiassa, Virginia; Sola, Claudia del Valle; Becerra, María Cecilia; Oxidative stress response in reference and clinical Staphylococcus aureus strains under Linezolid exposure; Elsevier; Journal of Global Antimicrobial Resistance; 22; 3-2020; 257-2622213-7165CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2213716520300618info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jgar.2020.02.032info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:34Zoai:ri.conicet.gov.ar:11336/111844instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:35.338CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oxidative stress response in reference and clinical Staphylococcus aureus strains under Linezolid exposure
title Oxidative stress response in reference and clinical Staphylococcus aureus strains under Linezolid exposure
spellingShingle Oxidative stress response in reference and clinical Staphylococcus aureus strains under Linezolid exposure
Martinez, Sol Romina
STAPHYLOCOCCUS AUREUS
OXIDATIVE STRESS
OXAZOLIDINONE
ANTIMICROBIAL RESISTANCE
title_short Oxidative stress response in reference and clinical Staphylococcus aureus strains under Linezolid exposure
title_full Oxidative stress response in reference and clinical Staphylococcus aureus strains under Linezolid exposure
title_fullStr Oxidative stress response in reference and clinical Staphylococcus aureus strains under Linezolid exposure
title_full_unstemmed Oxidative stress response in reference and clinical Staphylococcus aureus strains under Linezolid exposure
title_sort Oxidative stress response in reference and clinical Staphylococcus aureus strains under Linezolid exposure
dc.creator.none.fl_str_mv Martinez, Sol Romina
Aiassa, Virginia
Sola, Claudia del Valle
Becerra, María Cecilia
author Martinez, Sol Romina
author_facet Martinez, Sol Romina
Aiassa, Virginia
Sola, Claudia del Valle
Becerra, María Cecilia
author_role author
author2 Aiassa, Virginia
Sola, Claudia del Valle
Becerra, María Cecilia
author2_role author
author
author
dc.subject.none.fl_str_mv STAPHYLOCOCCUS AUREUS
OXIDATIVE STRESS
OXAZOLIDINONE
ANTIMICROBIAL RESISTANCE
topic STAPHYLOCOCCUS AUREUS
OXIDATIVE STRESS
OXAZOLIDINONE
ANTIMICROBIAL RESISTANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) strains are some of the most widespread pathogens multi-resistant to antimicrobial agents (AA). The AA provoke several changes inside bacteria, which cannot be solely explained by the main mechanisms of action reported. OBJECTIVE:The role of the oxidative stress in bacteria exposed to bacteriostatic AA has not been widely studied; hence, the aim of our work was to investigate the effect of Linezolid (LZD) on S. aureus strains. METHODS:Oxidative stress markers, such as superoxide dismutase enzyme (SOD) activity, the global antioxidant response, the advanced oxidation protein products (AOPP) and the basal levels of glutathione in 28 clinical and 2 reference strains were performed. RESULTS AND CONCLUSIONS:We identified 10 of 30 strains showing a slightly increase in reactive species under LZD treatment with respect to the untreated control (between 22 to 56%). The higher generation was detected in the clinical strains compared to the reference ones, however the impact in the antioxidant response was not significant, and the oxidized protein levels were almost undetectable. The strains exposed to this oxazolidinone did not suffer acute oxidative stress. This is the first work reporting the behavior of S. aureus clinical and reference strains exposed to LZD, being the oxidative stress negligible.
Fil: Martinez, Sol Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Aiassa, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina
Fil: Sola, Claudia del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
description BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) strains are some of the most widespread pathogens multi-resistant to antimicrobial agents (AA). The AA provoke several changes inside bacteria, which cannot be solely explained by the main mechanisms of action reported. OBJECTIVE:The role of the oxidative stress in bacteria exposed to bacteriostatic AA has not been widely studied; hence, the aim of our work was to investigate the effect of Linezolid (LZD) on S. aureus strains. METHODS:Oxidative stress markers, such as superoxide dismutase enzyme (SOD) activity, the global antioxidant response, the advanced oxidation protein products (AOPP) and the basal levels of glutathione in 28 clinical and 2 reference strains were performed. RESULTS AND CONCLUSIONS:We identified 10 of 30 strains showing a slightly increase in reactive species under LZD treatment with respect to the untreated control (between 22 to 56%). The higher generation was detected in the clinical strains compared to the reference ones, however the impact in the antioxidant response was not significant, and the oxidized protein levels were almost undetectable. The strains exposed to this oxazolidinone did not suffer acute oxidative stress. This is the first work reporting the behavior of S. aureus clinical and reference strains exposed to LZD, being the oxidative stress negligible.
publishDate 2020
dc.date.none.fl_str_mv 2020-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/111844
Martinez, Sol Romina; Aiassa, Virginia; Sola, Claudia del Valle; Becerra, María Cecilia; Oxidative stress response in reference and clinical Staphylococcus aureus strains under Linezolid exposure; Elsevier; Journal of Global Antimicrobial Resistance; 22; 3-2020; 257-262
2213-7165
CONICET Digital
CONICET
url http://hdl.handle.net/11336/111844
identifier_str_mv Martinez, Sol Romina; Aiassa, Virginia; Sola, Claudia del Valle; Becerra, María Cecilia; Oxidative stress response in reference and clinical Staphylococcus aureus strains under Linezolid exposure; Elsevier; Journal of Global Antimicrobial Resistance; 22; 3-2020; 257-262
2213-7165
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2213716520300618
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jgar.2020.02.032
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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