Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina

Autores
Lucero Estrada, Cecilia Stella Marys; Alcaráz, Lucia E.; Satorres, Sara E.; Manfredi, Eduardo; Velázquez, Lidia del Carmen
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An increase in the consumption of fruit juices and minimally processed fruits salads has been observed in recent years all over the world. In this work, the microbiological quality of artisan fruit salads was analysed. Faecal coliforms, Salmonella spp, Shigella spp, Yersinia enterocolitica and Escherichia coli O157:H7 were not detected; nevertheless, eleven strains of Staphylococcus aureus were isolated. By multiplex PCR, all isolates showed positive results for S. aureus 16S rRNA gene and 63.6% of them were positive for sea gene. Furthermore, PCR sea positive strains were able to produce the corresponding enterotoxin. Finally, the inactivation of these strains in fruit salads by nisin, lysozyme and EDTA, was studied. EDTA produced a total S. aureus growth inhibition after 60 h of incubation at a concentration of 250 mg/L. The presence of S. aureus might indicate inadequate hygiene conditions during salad elaboration; however, the enterotoxigenicity of the strains isolated in this study, highlights the risk of consumers intoxication. EDTA could be used to inhibit the growth of S. aureus in artisan fruit salads and extend the shelf life of these products.
Fil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alcaráz, Lucia E.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Satorres, Sara E.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Manfredi, Eduardo. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorio e Instituto de Salud ; Argentina
Fil: Velázquez, Lidia del Carmen. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Materia
STAPHYLOCOCCUS AUREUS,
ENTEROTOXIGENIC STRAINS
FRUIT SALADS
ANTIMICROBIAL COMPOUNDS
MULTIPLEX PCR
STAPHYLOCOCCUS AUREUS,
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41616
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41616
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, ArgentinaLucero Estrada, Cecilia Stella MarysAlcaráz, Lucia E.Satorres, Sara E.Manfredi, EduardoVelázquez, Lidia del CarmenSTAPHYLOCOCCUS AUREUS,ENTEROTOXIGENIC STRAINSFRUIT SALADSANTIMICROBIAL COMPOUNDSMULTIPLEX PCRSTAPHYLOCOCCUS AUREUS,https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1An increase in the consumption of fruit juices and minimally processed fruits salads has been observed in recent years all over the world. In this work, the microbiological quality of artisan fruit salads was analysed. Faecal coliforms, Salmonella spp, Shigella spp, Yersinia enterocolitica and Escherichia coli O157:H7 were not detected; nevertheless, eleven strains of Staphylococcus aureus were isolated. By multiplex PCR, all isolates showed positive results for S. aureus 16S rRNA gene and 63.6% of them were positive for sea gene. Furthermore, PCR sea positive strains were able to produce the corresponding enterotoxin. Finally, the inactivation of these strains in fruit salads by nisin, lysozyme and EDTA, was studied. EDTA produced a total S. aureus growth inhibition after 60 h of incubation at a concentration of 250 mg/L. The presence of S. aureus might indicate inadequate hygiene conditions during salad elaboration; however, the enterotoxigenicity of the strains isolated in this study, highlights the risk of consumers intoxication. EDTA could be used to inhibit the growth of S. aureus in artisan fruit salads and extend the shelf life of these products.Fil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alcaráz, Lucia E.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Satorres, Sara E.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Manfredi, Eduardo. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorio e Instituto de Salud ; ArgentinaFil: Velázquez, Lidia del Carmen. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaSociedade Brasileira de Microbiologia2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41616Lucero Estrada, Cecilia Stella Marys; Alcaráz, Lucia E.; Satorres, Sara E.; Manfredi, Eduardo; Velázquez, Lidia del Carmen; Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina; Sociedade Brasileira de Microbiologia; Brazilian Journal of Microbiology; 44; 4; 12-2013; 1155-11611517-83821678-4405CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/mr9gxvinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958181/info:eu-repo/semantics/altIdentifier/doi/10.1590/S1517-83822014005000001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:17Zoai:ri.conicet.gov.ar:11336/41616instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:17.772CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina
title Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina
spellingShingle Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina
Lucero Estrada, Cecilia Stella Marys
STAPHYLOCOCCUS AUREUS,
ENTEROTOXIGENIC STRAINS
FRUIT SALADS
ANTIMICROBIAL COMPOUNDS
MULTIPLEX PCR
STAPHYLOCOCCUS AUREUS,
title_short Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina
title_full Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina
title_fullStr Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina
title_full_unstemmed Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina
title_sort Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina
dc.creator.none.fl_str_mv Lucero Estrada, Cecilia Stella Marys
Alcaráz, Lucia E.
Satorres, Sara E.
Manfredi, Eduardo
Velázquez, Lidia del Carmen
author Lucero Estrada, Cecilia Stella Marys
author_facet Lucero Estrada, Cecilia Stella Marys
Alcaráz, Lucia E.
Satorres, Sara E.
Manfredi, Eduardo
Velázquez, Lidia del Carmen
author_role author
author2 Alcaráz, Lucia E.
Satorres, Sara E.
Manfredi, Eduardo
Velázquez, Lidia del Carmen
author2_role author
author
author
author
dc.subject.none.fl_str_mv STAPHYLOCOCCUS AUREUS,
ENTEROTOXIGENIC STRAINS
FRUIT SALADS
ANTIMICROBIAL COMPOUNDS
MULTIPLEX PCR
STAPHYLOCOCCUS AUREUS,
topic STAPHYLOCOCCUS AUREUS,
ENTEROTOXIGENIC STRAINS
FRUIT SALADS
ANTIMICROBIAL COMPOUNDS
MULTIPLEX PCR
STAPHYLOCOCCUS AUREUS,
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An increase in the consumption of fruit juices and minimally processed fruits salads has been observed in recent years all over the world. In this work, the microbiological quality of artisan fruit salads was analysed. Faecal coliforms, Salmonella spp, Shigella spp, Yersinia enterocolitica and Escherichia coli O157:H7 were not detected; nevertheless, eleven strains of Staphylococcus aureus were isolated. By multiplex PCR, all isolates showed positive results for S. aureus 16S rRNA gene and 63.6% of them were positive for sea gene. Furthermore, PCR sea positive strains were able to produce the corresponding enterotoxin. Finally, the inactivation of these strains in fruit salads by nisin, lysozyme and EDTA, was studied. EDTA produced a total S. aureus growth inhibition after 60 h of incubation at a concentration of 250 mg/L. The presence of S. aureus might indicate inadequate hygiene conditions during salad elaboration; however, the enterotoxigenicity of the strains isolated in this study, highlights the risk of consumers intoxication. EDTA could be used to inhibit the growth of S. aureus in artisan fruit salads and extend the shelf life of these products.
Fil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alcaráz, Lucia E.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Satorres, Sara E.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Manfredi, Eduardo. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorio e Instituto de Salud ; Argentina
Fil: Velázquez, Lidia del Carmen. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
description An increase in the consumption of fruit juices and minimally processed fruits salads has been observed in recent years all over the world. In this work, the microbiological quality of artisan fruit salads was analysed. Faecal coliforms, Salmonella spp, Shigella spp, Yersinia enterocolitica and Escherichia coli O157:H7 were not detected; nevertheless, eleven strains of Staphylococcus aureus were isolated. By multiplex PCR, all isolates showed positive results for S. aureus 16S rRNA gene and 63.6% of them were positive for sea gene. Furthermore, PCR sea positive strains were able to produce the corresponding enterotoxin. Finally, the inactivation of these strains in fruit salads by nisin, lysozyme and EDTA, was studied. EDTA produced a total S. aureus growth inhibition after 60 h of incubation at a concentration of 250 mg/L. The presence of S. aureus might indicate inadequate hygiene conditions during salad elaboration; however, the enterotoxigenicity of the strains isolated in this study, highlights the risk of consumers intoxication. EDTA could be used to inhibit the growth of S. aureus in artisan fruit salads and extend the shelf life of these products.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41616
Lucero Estrada, Cecilia Stella Marys; Alcaráz, Lucia E.; Satorres, Sara E.; Manfredi, Eduardo; Velázquez, Lidia del Carmen; Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina; Sociedade Brasileira de Microbiologia; Brazilian Journal of Microbiology; 44; 4; 12-2013; 1155-1161
1517-8382
1678-4405
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41616
identifier_str_mv Lucero Estrada, Cecilia Stella Marys; Alcaráz, Lucia E.; Satorres, Sara E.; Manfredi, Eduardo; Velázquez, Lidia del Carmen; Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina; Sociedade Brasileira de Microbiologia; Brazilian Journal of Microbiology; 44; 4; 12-2013; 1155-1161
1517-8382
1678-4405
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/mr9gxv
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3958181/
info:eu-repo/semantics/altIdentifier/doi/10.1590/S1517-83822014005000001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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