Effect of the association of reduced glutathione and ciprofloxacin on the antimicrobial activity in Staphylococcus aureus

Autores
Páez, Paulina Laura; Becerra, María Cecilia; Albesa, Inés
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We report the effect of glutathione and the role of reactive oxygen species (ROS), assayed by a nitro blue tetrazolium reaction, on the antibacterial action of ciprofloxacin, gentamicin and chloramphenicol in Staphylococcus aureus 22 resistant to ciprofloxacin and gentamicin, and in S. aureus ATCC 29213 sensitive to the above three antibiotics. The association of glutathione with ciprofloxacin or gentamicin significantly reduced the value of the minimum inhibitory concentration (MIC) in resistant S. aureus 22, measured using the macrodilution method, with a concomitant increase of intracellular ROS and a decrease of extracellular ROS. However, glutathione did not induce modifications in MIC or ROS generated by chloramphenicol. Furthermore, in the sensitive S. aureus ATCC 29213, the association of glutathione with ciprofloxacin, gentamicin or chloramphenicol did not induce any significant variations of MIC or ROS. There was a correlation between the stimulus of intracellular ROS and the decrease of MIC caused by exogenous glutathione. According to the results obtained, it is possible to modify the sensitivity of resistant strains of S. aureus by the addition of exogenous glutathione.
Fil: Páez, Paulina Laura. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Becerra, María Cecilia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Albesa, Inés. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Antibiotics
Oxidative Stress
Glutathione
Staphylococcus Aureus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24711
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of the association of reduced glutathione and ciprofloxacin on the antimicrobial activity in Staphylococcus aureusPáez, Paulina LauraBecerra, María CeciliaAlbesa, InésAntibioticsOxidative StressGlutathioneStaphylococcus Aureushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We report the effect of glutathione and the role of reactive oxygen species (ROS), assayed by a nitro blue tetrazolium reaction, on the antibacterial action of ciprofloxacin, gentamicin and chloramphenicol in Staphylococcus aureus 22 resistant to ciprofloxacin and gentamicin, and in S. aureus ATCC 29213 sensitive to the above three antibiotics. The association of glutathione with ciprofloxacin or gentamicin significantly reduced the value of the minimum inhibitory concentration (MIC) in resistant S. aureus 22, measured using the macrodilution method, with a concomitant increase of intracellular ROS and a decrease of extracellular ROS. However, glutathione did not induce modifications in MIC or ROS generated by chloramphenicol. Furthermore, in the sensitive S. aureus ATCC 29213, the association of glutathione with ciprofloxacin, gentamicin or chloramphenicol did not induce any significant variations of MIC or ROS. There was a correlation between the stimulus of intracellular ROS and the decrease of MIC caused by exogenous glutathione. According to the results obtained, it is possible to modify the sensitivity of resistant strains of S. aureus by the addition of exogenous glutathione.Fil: Páez, Paulina Laura. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Becerra, María Cecilia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Albesa, Inés. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaOxford University Press2010-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24711Páez, Paulina Laura; Becerra, María Cecilia; Albesa, Inés; Effect of the association of reduced glutathione and ciprofloxacin on the antimicrobial activity in Staphylococcus aureus; Oxford University Press; FEMS Microbiology Letters; 303; 1; 2-2010; 101-1050378-10971574-6968CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2009.01867.xinfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.2009.01867.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:50Zoai:ri.conicet.gov.ar:11336/24711instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:50.681CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of the association of reduced glutathione and ciprofloxacin on the antimicrobial activity in Staphylococcus aureus
title Effect of the association of reduced glutathione and ciprofloxacin on the antimicrobial activity in Staphylococcus aureus
spellingShingle Effect of the association of reduced glutathione and ciprofloxacin on the antimicrobial activity in Staphylococcus aureus
Páez, Paulina Laura
Antibiotics
Oxidative Stress
Glutathione
Staphylococcus Aureus
title_short Effect of the association of reduced glutathione and ciprofloxacin on the antimicrobial activity in Staphylococcus aureus
title_full Effect of the association of reduced glutathione and ciprofloxacin on the antimicrobial activity in Staphylococcus aureus
title_fullStr Effect of the association of reduced glutathione and ciprofloxacin on the antimicrobial activity in Staphylococcus aureus
title_full_unstemmed Effect of the association of reduced glutathione and ciprofloxacin on the antimicrobial activity in Staphylococcus aureus
title_sort Effect of the association of reduced glutathione and ciprofloxacin on the antimicrobial activity in Staphylococcus aureus
dc.creator.none.fl_str_mv Páez, Paulina Laura
Becerra, María Cecilia
Albesa, Inés
author Páez, Paulina Laura
author_facet Páez, Paulina Laura
Becerra, María Cecilia
Albesa, Inés
author_role author
author2 Becerra, María Cecilia
Albesa, Inés
author2_role author
author
dc.subject.none.fl_str_mv Antibiotics
Oxidative Stress
Glutathione
Staphylococcus Aureus
topic Antibiotics
Oxidative Stress
Glutathione
Staphylococcus Aureus
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We report the effect of glutathione and the role of reactive oxygen species (ROS), assayed by a nitro blue tetrazolium reaction, on the antibacterial action of ciprofloxacin, gentamicin and chloramphenicol in Staphylococcus aureus 22 resistant to ciprofloxacin and gentamicin, and in S. aureus ATCC 29213 sensitive to the above three antibiotics. The association of glutathione with ciprofloxacin or gentamicin significantly reduced the value of the minimum inhibitory concentration (MIC) in resistant S. aureus 22, measured using the macrodilution method, with a concomitant increase of intracellular ROS and a decrease of extracellular ROS. However, glutathione did not induce modifications in MIC or ROS generated by chloramphenicol. Furthermore, in the sensitive S. aureus ATCC 29213, the association of glutathione with ciprofloxacin, gentamicin or chloramphenicol did not induce any significant variations of MIC or ROS. There was a correlation between the stimulus of intracellular ROS and the decrease of MIC caused by exogenous glutathione. According to the results obtained, it is possible to modify the sensitivity of resistant strains of S. aureus by the addition of exogenous glutathione.
Fil: Páez, Paulina Laura. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Becerra, María Cecilia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Albesa, Inés. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description We report the effect of glutathione and the role of reactive oxygen species (ROS), assayed by a nitro blue tetrazolium reaction, on the antibacterial action of ciprofloxacin, gentamicin and chloramphenicol in Staphylococcus aureus 22 resistant to ciprofloxacin and gentamicin, and in S. aureus ATCC 29213 sensitive to the above three antibiotics. The association of glutathione with ciprofloxacin or gentamicin significantly reduced the value of the minimum inhibitory concentration (MIC) in resistant S. aureus 22, measured using the macrodilution method, with a concomitant increase of intracellular ROS and a decrease of extracellular ROS. However, glutathione did not induce modifications in MIC or ROS generated by chloramphenicol. Furthermore, in the sensitive S. aureus ATCC 29213, the association of glutathione with ciprofloxacin, gentamicin or chloramphenicol did not induce any significant variations of MIC or ROS. There was a correlation between the stimulus of intracellular ROS and the decrease of MIC caused by exogenous glutathione. According to the results obtained, it is possible to modify the sensitivity of resistant strains of S. aureus by the addition of exogenous glutathione.
publishDate 2010
dc.date.none.fl_str_mv 2010-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24711
Páez, Paulina Laura; Becerra, María Cecilia; Albesa, Inés; Effect of the association of reduced glutathione and ciprofloxacin on the antimicrobial activity in Staphylococcus aureus; Oxford University Press; FEMS Microbiology Letters; 303; 1; 2-2010; 101-105
0378-1097
1574-6968
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24711
identifier_str_mv Páez, Paulina Laura; Becerra, María Cecilia; Albesa, Inés; Effect of the association of reduced glutathione and ciprofloxacin on the antimicrobial activity in Staphylococcus aureus; Oxford University Press; FEMS Microbiology Letters; 303; 1; 2-2010; 101-105
0378-1097
1574-6968
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2009.01867.x
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.2009.01867.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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