SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells

Autores
Murthy, Vidya; Maison, Stéphane F.; Taranda, Julian; Haque, Nadeem; Bond, Chris T.; Elgoyhen, Ana Belen; Adelman, John P.; Liberman, M. Charles; Vetter, Douglas E.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cochlear hair cells use SK2 currents to shape responses to cholinergic efferent feedback from the brain. Using SK2-/- mice, we demonstrate that, in addition to their previously defined role in modulating hair cell membrane potentials, SK2 channels are necessary for long-term survival of olivocochlear fibers and synapses. Loss of the SK2 gene also results in loss of electrically driven olivocochlear effects in vivo, and down regulation of ryanodine receptors involved in calcium-induced calcium release, the main inducer of nAChR evoked SK2 activity. Generation of double-null mice lacking both the α10 nAChR gene, loss of which results in hypertrophied olivocochlear terminals, and the SK2 gene, recapitulates the SK2-/- synaptic phenotype and gene expression, and also leads to down regulation of α9 nAChR gene expression. The data suggest a hierarchy of activity necessary to maintain early olivocochlear synapses at their targets, with SK2 serving an epistatic, upstream, role to the nAChRs.
Fil: Murthy, Vidya. Tufts University; Estados Unidos
Fil: Maison, Stéphane F.. Massachusetts Eye and Ear Infirmary; Estados Unidos. Harvard Medical School; Estados Unidos
Fil: Taranda, Julian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Tufts University; Estados Unidos
Fil: Haque, Nadeem. University of Notre Dame; Estados Unidos
Fil: Bond, Chris T.. Oregon Health Sciences University; Estados Unidos
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Adelman, John P.. Oregon Health Sciences University; Estados Unidos
Fil: Liberman, M. Charles. Massachusetts Eye and Ear Infirmary; Estados Unidos. Harvard Medical School; Estados Unidos
Fil: Vetter, Douglas E.. Tufts University; Estados Unidos
Materia
COCHLEA
NICOTINIC RECEPTORS
SMALL CONDUCTANCE POTASSIUM CHANNELS
SYNAPTIC DEGENERATION
SYNAPTOGENESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79620
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/79620
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cellsMurthy, VidyaMaison, Stéphane F.Taranda, JulianHaque, NadeemBond, Chris T.Elgoyhen, Ana BelenAdelman, John P.Liberman, M. CharlesVetter, Douglas E.COCHLEANICOTINIC RECEPTORSSMALL CONDUCTANCE POTASSIUM CHANNELSSYNAPTIC DEGENERATIONSYNAPTOGENESIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cochlear hair cells use SK2 currents to shape responses to cholinergic efferent feedback from the brain. Using SK2-/- mice, we demonstrate that, in addition to their previously defined role in modulating hair cell membrane potentials, SK2 channels are necessary for long-term survival of olivocochlear fibers and synapses. Loss of the SK2 gene also results in loss of electrically driven olivocochlear effects in vivo, and down regulation of ryanodine receptors involved in calcium-induced calcium release, the main inducer of nAChR evoked SK2 activity. Generation of double-null mice lacking both the α10 nAChR gene, loss of which results in hypertrophied olivocochlear terminals, and the SK2 gene, recapitulates the SK2-/- synaptic phenotype and gene expression, and also leads to down regulation of α9 nAChR gene expression. The data suggest a hierarchy of activity necessary to maintain early olivocochlear synapses at their targets, with SK2 serving an epistatic, upstream, role to the nAChRs.Fil: Murthy, Vidya. Tufts University; Estados UnidosFil: Maison, Stéphane F.. Massachusetts Eye and Ear Infirmary; Estados Unidos. Harvard Medical School; Estados UnidosFil: Taranda, Julian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Tufts University; Estados UnidosFil: Haque, Nadeem. University of Notre Dame; Estados UnidosFil: Bond, Chris T.. Oregon Health Sciences University; Estados UnidosFil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Adelman, John P.. Oregon Health Sciences University; Estados UnidosFil: Liberman, M. Charles. Massachusetts Eye and Ear Infirmary; Estados Unidos. Harvard Medical School; Estados UnidosFil: Vetter, Douglas E.. Tufts University; Estados UnidosAcademic Press Inc Elsevier Science2009-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79620Murthy, Vidya; Maison, Stéphane F.; Taranda, Julian; Haque, Nadeem; Bond, Chris T.; et al.; SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells; Academic Press Inc Elsevier Science; Molecular and Cellular Neuroscience; 40; 1; 1-2009; 39-491044-7431CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18848895/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mcn.2008.08.011info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1044743108002431info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:49:35Zoai:ri.conicet.gov.ar:11336/79620instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:49:36.162CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells
title SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells
spellingShingle SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells
Murthy, Vidya
COCHLEA
NICOTINIC RECEPTORS
SMALL CONDUCTANCE POTASSIUM CHANNELS
SYNAPTIC DEGENERATION
SYNAPTOGENESIS
title_short SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells
title_full SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells
title_fullStr SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells
title_full_unstemmed SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells
title_sort SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells
dc.creator.none.fl_str_mv Murthy, Vidya
Maison, Stéphane F.
Taranda, Julian
Haque, Nadeem
Bond, Chris T.
Elgoyhen, Ana Belen
Adelman, John P.
Liberman, M. Charles
Vetter, Douglas E.
author Murthy, Vidya
author_facet Murthy, Vidya
Maison, Stéphane F.
Taranda, Julian
Haque, Nadeem
Bond, Chris T.
Elgoyhen, Ana Belen
Adelman, John P.
Liberman, M. Charles
Vetter, Douglas E.
author_role author
author2 Maison, Stéphane F.
Taranda, Julian
Haque, Nadeem
Bond, Chris T.
Elgoyhen, Ana Belen
Adelman, John P.
Liberman, M. Charles
Vetter, Douglas E.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COCHLEA
NICOTINIC RECEPTORS
SMALL CONDUCTANCE POTASSIUM CHANNELS
SYNAPTIC DEGENERATION
SYNAPTOGENESIS
topic COCHLEA
NICOTINIC RECEPTORS
SMALL CONDUCTANCE POTASSIUM CHANNELS
SYNAPTIC DEGENERATION
SYNAPTOGENESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cochlear hair cells use SK2 currents to shape responses to cholinergic efferent feedback from the brain. Using SK2-/- mice, we demonstrate that, in addition to their previously defined role in modulating hair cell membrane potentials, SK2 channels are necessary for long-term survival of olivocochlear fibers and synapses. Loss of the SK2 gene also results in loss of electrically driven olivocochlear effects in vivo, and down regulation of ryanodine receptors involved in calcium-induced calcium release, the main inducer of nAChR evoked SK2 activity. Generation of double-null mice lacking both the α10 nAChR gene, loss of which results in hypertrophied olivocochlear terminals, and the SK2 gene, recapitulates the SK2-/- synaptic phenotype and gene expression, and also leads to down regulation of α9 nAChR gene expression. The data suggest a hierarchy of activity necessary to maintain early olivocochlear synapses at their targets, with SK2 serving an epistatic, upstream, role to the nAChRs.
Fil: Murthy, Vidya. Tufts University; Estados Unidos
Fil: Maison, Stéphane F.. Massachusetts Eye and Ear Infirmary; Estados Unidos. Harvard Medical School; Estados Unidos
Fil: Taranda, Julian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Tufts University; Estados Unidos
Fil: Haque, Nadeem. University of Notre Dame; Estados Unidos
Fil: Bond, Chris T.. Oregon Health Sciences University; Estados Unidos
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Adelman, John P.. Oregon Health Sciences University; Estados Unidos
Fil: Liberman, M. Charles. Massachusetts Eye and Ear Infirmary; Estados Unidos. Harvard Medical School; Estados Unidos
Fil: Vetter, Douglas E.. Tufts University; Estados Unidos
description Cochlear hair cells use SK2 currents to shape responses to cholinergic efferent feedback from the brain. Using SK2-/- mice, we demonstrate that, in addition to their previously defined role in modulating hair cell membrane potentials, SK2 channels are necessary for long-term survival of olivocochlear fibers and synapses. Loss of the SK2 gene also results in loss of electrically driven olivocochlear effects in vivo, and down regulation of ryanodine receptors involved in calcium-induced calcium release, the main inducer of nAChR evoked SK2 activity. Generation of double-null mice lacking both the α10 nAChR gene, loss of which results in hypertrophied olivocochlear terminals, and the SK2 gene, recapitulates the SK2-/- synaptic phenotype and gene expression, and also leads to down regulation of α9 nAChR gene expression. The data suggest a hierarchy of activity necessary to maintain early olivocochlear synapses at their targets, with SK2 serving an epistatic, upstream, role to the nAChRs.
publishDate 2009
dc.date.none.fl_str_mv 2009-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79620
Murthy, Vidya; Maison, Stéphane F.; Taranda, Julian; Haque, Nadeem; Bond, Chris T.; et al.; SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells; Academic Press Inc Elsevier Science; Molecular and Cellular Neuroscience; 40; 1; 1-2009; 39-49
1044-7431
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79620
identifier_str_mv Murthy, Vidya; Maison, Stéphane F.; Taranda, Julian; Haque, Nadeem; Bond, Chris T.; et al.; SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells; Academic Press Inc Elsevier Science; Molecular and Cellular Neuroscience; 40; 1; 1-2009; 39-49
1044-7431
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18848895/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mcn.2008.08.011
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1044743108002431
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083018612015104
score 13.22299

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