A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses

Autores
Wedemeyer, Carolina; Vattino, Lucas Gabriel; Ballestero, Jimena Andrea; Katz, Eleonora; Elgoyhen, Ana Belen
Año de publicación
2015
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
IHCs convey acoustic information to the central nervous system while OHCs are responsible for the mechanical amplification of sound. IHCs receive a transient MOC innervation since birth to the onset of hearing, while MOC fibers synapse onto OHCs from the first postnatal week throughout adulthood. The MOC-HC synapse is inhibitory and mediated by a9a10 nicotinic receptors (nAChRs). We analyzed the properties of synaptic transmission of a knock-in mouse (Kin) with a point mutation in the a9 nAChR subunit (L9´T) that prolongs MOC inhibition (Taranda et. al 2009). Synaptic currents (IPSCs) were recorded in IHCs and OHCs of isolated mouse cochleas at postnatal day 9-13 during electrical stimulation of MOC fibers. In previous studies we showed that high frequency stimulation causes synaptic depression in MOC-IHC synapses, whereas it causes facilitation in MOC-OHC synapses. We found that in both wt and Kin IHCs, 100Hz-trains applied to the MOC fibers caused depression of IPSC amplitudes (S10/S1: 21% and 10% in Kin and wt mice, respectively) whereas 10Hz-trains caused depression only in Kin mice (S10/S1:60%). Accordingly, the ready releasable pool size was smaller in Kin mice (wt:3.7±0.9 Kin:2.7±0.8). Preliminary experiments in OHCs show that high frequency stimulation (40-80 Hz) caused 3-fold more facilitation in Kin than in wt mice. These results show that a modification in the postsynaptic nAChR alters the short term plasticity pattern of MOC-HC synapses.
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Vattino, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Ballestero, Jimena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología. 3º Cátedra de Farmacología; Argentina
XXX Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; ISN Small Conference and Course
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Neurosciencias
Materia
HAIR CELL
COCHLEA
POINT MUTATION
SYNAPTIC PLASTICITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215100
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/215100
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapsesWedemeyer, CarolinaVattino, Lucas GabrielBallestero, Jimena AndreaKatz, EleonoraElgoyhen, Ana BelenHAIR CELLCOCHLEAPOINT MUTATIONSYNAPTIC PLASTICITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1IHCs convey acoustic information to the central nervous system while OHCs are responsible for the mechanical amplification of sound. IHCs receive a transient MOC innervation since birth to the onset of hearing, while MOC fibers synapse onto OHCs from the first postnatal week throughout adulthood. The MOC-HC synapse is inhibitory and mediated by a9a10 nicotinic receptors (nAChRs). We analyzed the properties of synaptic transmission of a knock-in mouse (Kin) with a point mutation in the a9 nAChR subunit (L9´T) that prolongs MOC inhibition (Taranda et. al 2009). Synaptic currents (IPSCs) were recorded in IHCs and OHCs of isolated mouse cochleas at postnatal day 9-13 during electrical stimulation of MOC fibers. In previous studies we showed that high frequency stimulation causes synaptic depression in MOC-IHC synapses, whereas it causes facilitation in MOC-OHC synapses. We found that in both wt and Kin IHCs, 100Hz-trains applied to the MOC fibers caused depression of IPSC amplitudes (S10/S1: 21% and 10% in Kin and wt mice, respectively) whereas 10Hz-trains caused depression only in Kin mice (S10/S1:60%). Accordingly, the ready releasable pool size was smaller in Kin mice (wt:3.7±0.9 Kin:2.7±0.8). Preliminary experiments in OHCs show that high frequency stimulation (40-80 Hz) caused 3-fold more facilitation in Kin than in wt mice. These results show that a modification in the postsynaptic nAChR alters the short term plasticity pattern of MOC-HC synapses.Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Vattino, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Ballestero, Jimena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología. 3º Cátedra de Farmacología; ArgentinaXXX Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; ISN Small Conference and CourseMar del PlataArgentinaSociedad Argentina de Investigación en NeuroscienciasSociedad Argentina de Investigación en Neurociencias2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215100A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses; XXX Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; ISN Small Conference and Course; Mar del Plata; Argentina; 2015; 337-337CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/wp-content/uploads/2022/01/SAN2015_Mar-del-Plata._libro-de-resumenes.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:34:01Zoai:ri.conicet.gov.ar:11336/215100instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:34:01.746CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses
title A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses
spellingShingle A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses
Wedemeyer, Carolina
HAIR CELL
COCHLEA
POINT MUTATION
SYNAPTIC PLASTICITY
title_short A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses
title_full A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses
title_fullStr A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses
title_full_unstemmed A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses
title_sort A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses
dc.creator.none.fl_str_mv Wedemeyer, Carolina
Vattino, Lucas Gabriel
Ballestero, Jimena Andrea
Katz, Eleonora
Elgoyhen, Ana Belen
author Wedemeyer, Carolina
author_facet Wedemeyer, Carolina
Vattino, Lucas Gabriel
Ballestero, Jimena Andrea
Katz, Eleonora
Elgoyhen, Ana Belen
author_role author
author2 Vattino, Lucas Gabriel
Ballestero, Jimena Andrea
Katz, Eleonora
Elgoyhen, Ana Belen
author2_role author
author
author
author
dc.subject.none.fl_str_mv HAIR CELL
COCHLEA
POINT MUTATION
SYNAPTIC PLASTICITY
topic HAIR CELL
COCHLEA
POINT MUTATION
SYNAPTIC PLASTICITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv IHCs convey acoustic information to the central nervous system while OHCs are responsible for the mechanical amplification of sound. IHCs receive a transient MOC innervation since birth to the onset of hearing, while MOC fibers synapse onto OHCs from the first postnatal week throughout adulthood. The MOC-HC synapse is inhibitory and mediated by a9a10 nicotinic receptors (nAChRs). We analyzed the properties of synaptic transmission of a knock-in mouse (Kin) with a point mutation in the a9 nAChR subunit (L9´T) that prolongs MOC inhibition (Taranda et. al 2009). Synaptic currents (IPSCs) were recorded in IHCs and OHCs of isolated mouse cochleas at postnatal day 9-13 during electrical stimulation of MOC fibers. In previous studies we showed that high frequency stimulation causes synaptic depression in MOC-IHC synapses, whereas it causes facilitation in MOC-OHC synapses. We found that in both wt and Kin IHCs, 100Hz-trains applied to the MOC fibers caused depression of IPSC amplitudes (S10/S1: 21% and 10% in Kin and wt mice, respectively) whereas 10Hz-trains caused depression only in Kin mice (S10/S1:60%). Accordingly, the ready releasable pool size was smaller in Kin mice (wt:3.7±0.9 Kin:2.7±0.8). Preliminary experiments in OHCs show that high frequency stimulation (40-80 Hz) caused 3-fold more facilitation in Kin than in wt mice. These results show that a modification in the postsynaptic nAChR alters the short term plasticity pattern of MOC-HC synapses.
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Vattino, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Ballestero, Jimena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología. 3º Cátedra de Farmacología; Argentina
XXX Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; ISN Small Conference and Course
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Neurosciencias
description IHCs convey acoustic information to the central nervous system while OHCs are responsible for the mechanical amplification of sound. IHCs receive a transient MOC innervation since birth to the onset of hearing, while MOC fibers synapse onto OHCs from the first postnatal week throughout adulthood. The MOC-HC synapse is inhibitory and mediated by a9a10 nicotinic receptors (nAChRs). We analyzed the properties of synaptic transmission of a knock-in mouse (Kin) with a point mutation in the a9 nAChR subunit (L9´T) that prolongs MOC inhibition (Taranda et. al 2009). Synaptic currents (IPSCs) were recorded in IHCs and OHCs of isolated mouse cochleas at postnatal day 9-13 during electrical stimulation of MOC fibers. In previous studies we showed that high frequency stimulation causes synaptic depression in MOC-IHC synapses, whereas it causes facilitation in MOC-OHC synapses. We found that in both wt and Kin IHCs, 100Hz-trains applied to the MOC fibers caused depression of IPSC amplitudes (S10/S1: 21% and 10% in Kin and wt mice, respectively) whereas 10Hz-trains caused depression only in Kin mice (S10/S1:60%). Accordingly, the ready releasable pool size was smaller in Kin mice (wt:3.7±0.9 Kin:2.7±0.8). Preliminary experiments in OHCs show that high frequency stimulation (40-80 Hz) caused 3-fold more facilitation in Kin than in wt mice. These results show that a modification in the postsynaptic nAChR alters the short term plasticity pattern of MOC-HC synapses.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215100
A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses; XXX Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; ISN Small Conference and Course; Mar del Plata; Argentina; 2015; 337-337
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215100
identifier_str_mv A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses; XXX Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; ISN Small Conference and Course; Mar del Plata; Argentina; 2015; 337-337
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/wp-content/uploads/2022/01/SAN2015_Mar-del-Plata._libro-de-resumenes.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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