A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses
- Autores
- Wedemeyer, Carolina; Vattino, Lucas Gabriel; Ballestero, Jimena Andrea; Katz, Eleonora; Elgoyhen, Ana Belen
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- IHCs convey acoustic information to the central nervous system while OHCs are responsible for the mechanical amplification of sound. IHCs receive a transient MOC innervation since birth to the onset of hearing, while MOC fibers synapse onto OHCs from the first postnatal week throughout adulthood. The MOC-HC synapse is inhibitory and mediated by a9a10 nicotinic receptors (nAChRs). We analyzed the properties of synaptic transmission of a knock-in mouse (Kin) with a point mutation in the a9 nAChR subunit (L9´T) that prolongs MOC inhibition (Taranda et. al 2009). Synaptic currents (IPSCs) were recorded in IHCs and OHCs of isolated mouse cochleas at postnatal day 9-13 during electrical stimulation of MOC fibers. In previous studies we showed that high frequency stimulation causes synaptic depression in MOC-IHC synapses, whereas it causes facilitation in MOC-OHC synapses. We found that in both wt and Kin IHCs, 100Hz-trains applied to the MOC fibers caused depression of IPSC amplitudes (S10/S1: 21% and 10% in Kin and wt mice, respectively) whereas 10Hz-trains caused depression only in Kin mice (S10/S1:60%). Accordingly, the ready releasable pool size was smaller in Kin mice (wt:3.7±0.9 Kin:2.7±0.8). Preliminary experiments in OHCs show that high frequency stimulation (40-80 Hz) caused 3-fold more facilitation in Kin than in wt mice. These results show that a modification in the postsynaptic nAChR alters the short term plasticity pattern of MOC-HC synapses.
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Vattino, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Ballestero, Jimena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología. 3º Cátedra de Farmacología; Argentina
XXX Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; ISN Small Conference and Course
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Neurosciencias - Materia
-
HAIR CELL
COCHLEA
POINT MUTATION
SYNAPTIC PLASTICITY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/215100
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A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapsesWedemeyer, CarolinaVattino, Lucas GabrielBallestero, Jimena AndreaKatz, EleonoraElgoyhen, Ana BelenHAIR CELLCOCHLEAPOINT MUTATIONSYNAPTIC PLASTICITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1IHCs convey acoustic information to the central nervous system while OHCs are responsible for the mechanical amplification of sound. IHCs receive a transient MOC innervation since birth to the onset of hearing, while MOC fibers synapse onto OHCs from the first postnatal week throughout adulthood. The MOC-HC synapse is inhibitory and mediated by a9a10 nicotinic receptors (nAChRs). We analyzed the properties of synaptic transmission of a knock-in mouse (Kin) with a point mutation in the a9 nAChR subunit (L9´T) that prolongs MOC inhibition (Taranda et. al 2009). Synaptic currents (IPSCs) were recorded in IHCs and OHCs of isolated mouse cochleas at postnatal day 9-13 during electrical stimulation of MOC fibers. In previous studies we showed that high frequency stimulation causes synaptic depression in MOC-IHC synapses, whereas it causes facilitation in MOC-OHC synapses. We found that in both wt and Kin IHCs, 100Hz-trains applied to the MOC fibers caused depression of IPSC amplitudes (S10/S1: 21% and 10% in Kin and wt mice, respectively) whereas 10Hz-trains caused depression only in Kin mice (S10/S1:60%). Accordingly, the ready releasable pool size was smaller in Kin mice (wt:3.7±0.9 Kin:2.7±0.8). Preliminary experiments in OHCs show that high frequency stimulation (40-80 Hz) caused 3-fold more facilitation in Kin than in wt mice. These results show that a modification in the postsynaptic nAChR alters the short term plasticity pattern of MOC-HC synapses.Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Vattino, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Ballestero, Jimena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología. 3º Cátedra de Farmacología; ArgentinaXXX Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; ISN Small Conference and CourseMar del PlataArgentinaSociedad Argentina de Investigación en NeuroscienciasSociedad Argentina de Investigación en Neurociencias2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215100A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses; XXX Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; ISN Small Conference and Course; Mar del Plata; Argentina; 2015; 337-337CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/wp-content/uploads/2022/01/SAN2015_Mar-del-Plata._libro-de-resumenes.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:25Zoai:ri.conicet.gov.ar:11336/215100instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:25.931CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses |
title |
A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses |
spellingShingle |
A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses Wedemeyer, Carolina HAIR CELL COCHLEA POINT MUTATION SYNAPTIC PLASTICITY |
title_short |
A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses |
title_full |
A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses |
title_fullStr |
A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses |
title_full_unstemmed |
A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses |
title_sort |
A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses |
dc.creator.none.fl_str_mv |
Wedemeyer, Carolina Vattino, Lucas Gabriel Ballestero, Jimena Andrea Katz, Eleonora Elgoyhen, Ana Belen |
author |
Wedemeyer, Carolina |
author_facet |
Wedemeyer, Carolina Vattino, Lucas Gabriel Ballestero, Jimena Andrea Katz, Eleonora Elgoyhen, Ana Belen |
author_role |
author |
author2 |
Vattino, Lucas Gabriel Ballestero, Jimena Andrea Katz, Eleonora Elgoyhen, Ana Belen |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
HAIR CELL COCHLEA POINT MUTATION SYNAPTIC PLASTICITY |
topic |
HAIR CELL COCHLEA POINT MUTATION SYNAPTIC PLASTICITY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
IHCs convey acoustic information to the central nervous system while OHCs are responsible for the mechanical amplification of sound. IHCs receive a transient MOC innervation since birth to the onset of hearing, while MOC fibers synapse onto OHCs from the first postnatal week throughout adulthood. The MOC-HC synapse is inhibitory and mediated by a9a10 nicotinic receptors (nAChRs). We analyzed the properties of synaptic transmission of a knock-in mouse (Kin) with a point mutation in the a9 nAChR subunit (L9´T) that prolongs MOC inhibition (Taranda et. al 2009). Synaptic currents (IPSCs) were recorded in IHCs and OHCs of isolated mouse cochleas at postnatal day 9-13 during electrical stimulation of MOC fibers. In previous studies we showed that high frequency stimulation causes synaptic depression in MOC-IHC synapses, whereas it causes facilitation in MOC-OHC synapses. We found that in both wt and Kin IHCs, 100Hz-trains applied to the MOC fibers caused depression of IPSC amplitudes (S10/S1: 21% and 10% in Kin and wt mice, respectively) whereas 10Hz-trains caused depression only in Kin mice (S10/S1:60%). Accordingly, the ready releasable pool size was smaller in Kin mice (wt:3.7±0.9 Kin:2.7±0.8). Preliminary experiments in OHCs show that high frequency stimulation (40-80 Hz) caused 3-fold more facilitation in Kin than in wt mice. These results show that a modification in the postsynaptic nAChR alters the short term plasticity pattern of MOC-HC synapses. Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Vattino, Lucas Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Ballestero, Jimena Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología. 3º Cátedra de Farmacología; Argentina XXX Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; ISN Small Conference and Course Mar del Plata Argentina Sociedad Argentina de Investigación en Neurosciencias |
description |
IHCs convey acoustic information to the central nervous system while OHCs are responsible for the mechanical amplification of sound. IHCs receive a transient MOC innervation since birth to the onset of hearing, while MOC fibers synapse onto OHCs from the first postnatal week throughout adulthood. The MOC-HC synapse is inhibitory and mediated by a9a10 nicotinic receptors (nAChRs). We analyzed the properties of synaptic transmission of a knock-in mouse (Kin) with a point mutation in the a9 nAChR subunit (L9´T) that prolongs MOC inhibition (Taranda et. al 2009). Synaptic currents (IPSCs) were recorded in IHCs and OHCs of isolated mouse cochleas at postnatal day 9-13 during electrical stimulation of MOC fibers. In previous studies we showed that high frequency stimulation causes synaptic depression in MOC-IHC synapses, whereas it causes facilitation in MOC-OHC synapses. We found that in both wt and Kin IHCs, 100Hz-trains applied to the MOC fibers caused depression of IPSC amplitudes (S10/S1: 21% and 10% in Kin and wt mice, respectively) whereas 10Hz-trains caused depression only in Kin mice (S10/S1:60%). Accordingly, the ready releasable pool size was smaller in Kin mice (wt:3.7±0.9 Kin:2.7±0.8). Preliminary experiments in OHCs show that high frequency stimulation (40-80 Hz) caused 3-fold more facilitation in Kin than in wt mice. These results show that a modification in the postsynaptic nAChR alters the short term plasticity pattern of MOC-HC synapses. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/215100 A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses; XXX Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; ISN Small Conference and Course; Mar del Plata; Argentina; 2015; 337-337 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/215100 |
identifier_str_mv |
A point mutation in the alpha9alpha10 nAChR alters short-term synaptic plasticity of medial olivocochlear- hair cell (MOC-HC) synapses; XXX Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; ISN Small Conference and Course; Mar del Plata; Argentina; 2015; 337-337 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/wp-content/uploads/2022/01/SAN2015_Mar-del-Plata._libro-de-resumenes.pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Nacional |
dc.publisher.none.fl_str_mv |
Sociedad Argentina de Investigación en Neurociencias |
publisher.none.fl_str_mv |
Sociedad Argentina de Investigación en Neurociencias |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |