Activation of presynaptic GABAB receptors enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses

Autores
Wedemeyer, Carolina; Elgoyhen, Ana Belen; Katz, Eleonora
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
During development, medial olivocochlear (MOC) neurons transiently innervate cochlear inner hair cells (IHCs). Although acetylcholine is the main neurotransmitter at this synapse, an abundant GABA innervation is also present. Electrical stimulation of efferent fibers triggers the release of acetylcholine, but also activates presynaptic GABAB receptors, that in turn reduce the amount of ACh release. The mechanism of action of GABA is through the inhibition of P/Q type Ca2+ channels. We are now studying the consequences of GABAB-mediated inhibition in the short-term plasticity properties of this synapse. Inhibitory synaptic currents (IPSC) were recorded in IHCs of acutely isolated organs of Corti at P9-P11 while MOC fibers were electrically stimulated. In control conditions, 15 pulses applied at high frequency (50 or 100 Hz) resulted in a progressive decrease in IPSC amplitudes. At 50 Hz, the depression rate was 32% whereas it increased to 53% at 100 Hz. At low-frequency (10 Hz), the specific GABAB agonist, baclofen, reduced the amplitude of IPSCs throughout the train. However, at higher stimulus rates, IPSCs were always larger than controls with a maximal enhancement of 43% for the last IPSC of the train at 100 Hz. These results suggest that by reducing synaptic depression, activation of GABAB receptors would enable sustained transmission during high-frequency stimulation at the MOC-inner hair cell synapse.
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
XXIX Annual Meeting and SAN-ISN Small Conference and Course
Huerta Grande
Argentina
Sociedad Argentina de Investigación en Neurosciencias
Materia
COCHLEA
INNER HAIR CELLS
SYNAPTIC PLASTICITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215104
Acceder
id CONICETDig_b0376f11a14ff54e5cf42fae412dd9cb
oai_identifier_str oai:ri.conicet.gov.ar:11336/215104
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Activation of presynaptic GABAB receptors enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapsesWedemeyer, CarolinaElgoyhen, Ana BelenKatz, EleonoraCOCHLEAINNER HAIR CELLSSYNAPTIC PLASTICITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1During development, medial olivocochlear (MOC) neurons transiently innervate cochlear inner hair cells (IHCs). Although acetylcholine is the main neurotransmitter at this synapse, an abundant GABA innervation is also present. Electrical stimulation of efferent fibers triggers the release of acetylcholine, but also activates presynaptic GABAB receptors, that in turn reduce the amount of ACh release. The mechanism of action of GABA is through the inhibition of P/Q type Ca2+ channels. We are now studying the consequences of GABAB-mediated inhibition in the short-term plasticity properties of this synapse. Inhibitory synaptic currents (IPSC) were recorded in IHCs of acutely isolated organs of Corti at P9-P11 while MOC fibers were electrically stimulated. In control conditions, 15 pulses applied at high frequency (50 or 100 Hz) resulted in a progressive decrease in IPSC amplitudes. At 50 Hz, the depression rate was 32% whereas it increased to 53% at 100 Hz. At low-frequency (10 Hz), the specific GABAB agonist, baclofen, reduced the amplitude of IPSCs throughout the train. However, at higher stimulus rates, IPSCs were always larger than controls with a maximal enhancement of 43% for the last IPSC of the train at 100 Hz. These results suggest that by reducing synaptic depression, activation of GABAB receptors would enable sustained transmission during high-frequency stimulation at the MOC-inner hair cell synapse.Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaXXIX Annual Meeting and SAN-ISN Small Conference and CourseHuerta GrandeArgentinaSociedad Argentina de Investigación en NeuroscienciasSociedad Argentina de Investigación en Neurociencias2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/215104Activation of presynaptic GABAB receptors enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses; XXIX Annual Meeting and SAN-ISN Small Conference and Course; Huerta Grande; Argentina; 2014; 1-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.saneurociencias.org.ar/wp-content/uploads/2012/12/Booklet_San2014_Digital.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:15:58Zoai:ri.conicet.gov.ar:11336/215104instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:15:59.104CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Activation of presynaptic GABAB receptors enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
title Activation of presynaptic GABAB receptors enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
spellingShingle Activation of presynaptic GABAB receptors enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
Wedemeyer, Carolina
COCHLEA
INNER HAIR CELLS
SYNAPTIC PLASTICITY
title_short Activation of presynaptic GABAB receptors enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
title_full Activation of presynaptic GABAB receptors enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
title_fullStr Activation of presynaptic GABAB receptors enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
title_full_unstemmed Activation of presynaptic GABAB receptors enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
title_sort Activation of presynaptic GABAB receptors enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
dc.creator.none.fl_str_mv Wedemeyer, Carolina
Elgoyhen, Ana Belen
Katz, Eleonora
author Wedemeyer, Carolina
author_facet Wedemeyer, Carolina
Elgoyhen, Ana Belen
Katz, Eleonora
author_role author
author2 Elgoyhen, Ana Belen
Katz, Eleonora
author2_role author
author
dc.subject.none.fl_str_mv COCHLEA
INNER HAIR CELLS
SYNAPTIC PLASTICITY
topic COCHLEA
INNER HAIR CELLS
SYNAPTIC PLASTICITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv During development, medial olivocochlear (MOC) neurons transiently innervate cochlear inner hair cells (IHCs). Although acetylcholine is the main neurotransmitter at this synapse, an abundant GABA innervation is also present. Electrical stimulation of efferent fibers triggers the release of acetylcholine, but also activates presynaptic GABAB receptors, that in turn reduce the amount of ACh release. The mechanism of action of GABA is through the inhibition of P/Q type Ca2+ channels. We are now studying the consequences of GABAB-mediated inhibition in the short-term plasticity properties of this synapse. Inhibitory synaptic currents (IPSC) were recorded in IHCs of acutely isolated organs of Corti at P9-P11 while MOC fibers were electrically stimulated. In control conditions, 15 pulses applied at high frequency (50 or 100 Hz) resulted in a progressive decrease in IPSC amplitudes. At 50 Hz, the depression rate was 32% whereas it increased to 53% at 100 Hz. At low-frequency (10 Hz), the specific GABAB agonist, baclofen, reduced the amplitude of IPSCs throughout the train. However, at higher stimulus rates, IPSCs were always larger than controls with a maximal enhancement of 43% for the last IPSC of the train at 100 Hz. These results suggest that by reducing synaptic depression, activation of GABAB receptors would enable sustained transmission during high-frequency stimulation at the MOC-inner hair cell synapse.
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
XXIX Annual Meeting and SAN-ISN Small Conference and Course
Huerta Grande
Argentina
Sociedad Argentina de Investigación en Neurosciencias
description During development, medial olivocochlear (MOC) neurons transiently innervate cochlear inner hair cells (IHCs). Although acetylcholine is the main neurotransmitter at this synapse, an abundant GABA innervation is also present. Electrical stimulation of efferent fibers triggers the release of acetylcholine, but also activates presynaptic GABAB receptors, that in turn reduce the amount of ACh release. The mechanism of action of GABA is through the inhibition of P/Q type Ca2+ channels. We are now studying the consequences of GABAB-mediated inhibition in the short-term plasticity properties of this synapse. Inhibitory synaptic currents (IPSC) were recorded in IHCs of acutely isolated organs of Corti at P9-P11 while MOC fibers were electrically stimulated. In control conditions, 15 pulses applied at high frequency (50 or 100 Hz) resulted in a progressive decrease in IPSC amplitudes. At 50 Hz, the depression rate was 32% whereas it increased to 53% at 100 Hz. At low-frequency (10 Hz), the specific GABAB agonist, baclofen, reduced the amplitude of IPSCs throughout the train. However, at higher stimulus rates, IPSCs were always larger than controls with a maximal enhancement of 43% for the last IPSC of the train at 100 Hz. These results suggest that by reducing synaptic depression, activation of GABAB receptors would enable sustained transmission during high-frequency stimulation at the MOC-inner hair cell synapse.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215104
Activation of presynaptic GABAB receptors enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses; XXIX Annual Meeting and SAN-ISN Small Conference and Course; Huerta Grande; Argentina; 2014; 1-1
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215104
identifier_str_mv Activation of presynaptic GABAB receptors enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses; XXIX Annual Meeting and SAN-ISN Small Conference and Course; Huerta Grande; Argentina; 2014; 1-1
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.saneurociencias.org.ar/wp-content/uploads/2012/12/Booklet_San2014_Digital.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980865681915904
score 12.993085

Publicaciones similares