Activation of presynaptic GABAB receptors minimize depression and enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses

Autores
Wedemeyer, Carolina; Katz, Eleonora; Elgoyhen, Ana Belen
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
During development, medial olivocochlear (MOC) neurons transiently innervate cochlear inner hair cells (IHCs). Although acetylcholine (ACh) is the main neurotransmitter at this synapse, an abundant GABA innervation is also present. Electrical stimulation of MOC efferent fibers triggers the release of ACh, but also activates presynaptic GABAB receptors, that in turn reduce the amount of ACh release. GABA-mediated mechanism is through the inhibition of P/Q type Ca2+ channels. We are now studying the consequences of GABAB-mediated inhibition in the short-term plasticity of this synapse. Inhibitory synaptic currents (IPSC) were recorded in IHCs of acutely isolated organs of Corti at P9-P11 while MOC fibers were electrically stimulated. In control condition, 10 pulses applied at high frequency (50 Hz) resulted in a progressive decrease on IPSC amplitudes throughout the train (P10/P1= 0.54). Contrary, specific GABAB agonist, baclofen, increased facilitation rate and eliminated depression at the same frequency (P10/P1= 1). Moreover, application of CGP35348, a GABAB antagonist, produced a bigger depression even at low stimulation frequencies (10Hz). These results suggest that the activation of presynaptic GABAB receptors, minimizes depression and would enable sustained transmission during high-frequency stimulation at the MOC-inner hair cell synapse.
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
XXXII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Neurociencias
Materia
GABAB RECEPTOR
EFFERENT MODULATION
COCHLEA
SYNAPTIC PLATICITY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215097
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/215097
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Activation of presynaptic GABAB receptors minimize depression and enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapsesWedemeyer, CarolinaKatz, EleonoraElgoyhen, Ana BelenGABAB RECEPTOREFFERENT MODULATIONCOCHLEASYNAPTIC PLATICITYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1During development, medial olivocochlear (MOC) neurons transiently innervate cochlear inner hair cells (IHCs). Although acetylcholine (ACh) is the main neurotransmitter at this synapse, an abundant GABA innervation is also present. Electrical stimulation of MOC efferent fibers triggers the release of ACh, but also activates presynaptic GABAB receptors, that in turn reduce the amount of ACh release. GABA-mediated mechanism is through the inhibition of P/Q type Ca2+ channels. We are now studying the consequences of GABAB-mediated inhibition in the short-term plasticity of this synapse. Inhibitory synaptic currents (IPSC) were recorded in IHCs of acutely isolated organs of Corti at P9-P11 while MOC fibers were electrically stimulated. In control condition, 10 pulses applied at high frequency (50 Hz) resulted in a progressive decrease on IPSC amplitudes throughout the train (P10/P1= 0.54). Contrary, specific GABAB agonist, baclofen, increased facilitation rate and eliminated depression at the same frequency (P10/P1= 1). Moreover, application of CGP35348, a GABAB antagonist, produced a bigger depression even at low stimulation frequencies (10Hz). These results suggest that the activation of presynaptic GABAB receptors, minimizes depression and would enable sustained transmission during high-frequency stimulation at the MOC-inner hair cell synapse.Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; ArgentinaFil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; ArgentinaXXXII Congreso Anual de la Sociedad Argentina de Investigación en NeurocienciaMar del PlataArgentinaSociedad Argentina de Investigación en NeurocienciasSociedad Argentina de Investigación en Neurociencia2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215097Activation of presynaptic GABAB receptors minimize depression and enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses; XXXII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; Mar del Plata; Argentina; 2017; 1-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.saneurociencias.org.ar/san2017-abstract-book/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:29Zoai:ri.conicet.gov.ar:11336/215097instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:29.493CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Activation of presynaptic GABAB receptors minimize depression and enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
title Activation of presynaptic GABAB receptors minimize depression and enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
spellingShingle Activation of presynaptic GABAB receptors minimize depression and enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
Wedemeyer, Carolina
GABAB RECEPTOR
EFFERENT MODULATION
COCHLEA
SYNAPTIC PLATICITY
title_short Activation of presynaptic GABAB receptors minimize depression and enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
title_full Activation of presynaptic GABAB receptors minimize depression and enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
title_fullStr Activation of presynaptic GABAB receptors minimize depression and enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
title_full_unstemmed Activation of presynaptic GABAB receptors minimize depression and enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
title_sort Activation of presynaptic GABAB receptors minimize depression and enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
dc.creator.none.fl_str_mv Wedemeyer, Carolina
Katz, Eleonora
Elgoyhen, Ana Belen
author Wedemeyer, Carolina
author_facet Wedemeyer, Carolina
Katz, Eleonora
Elgoyhen, Ana Belen
author_role author
author2 Katz, Eleonora
Elgoyhen, Ana Belen
author2_role author
author
dc.subject.none.fl_str_mv GABAB RECEPTOR
EFFERENT MODULATION
COCHLEA
SYNAPTIC PLATICITY
topic GABAB RECEPTOR
EFFERENT MODULATION
COCHLEA
SYNAPTIC PLATICITY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv During development, medial olivocochlear (MOC) neurons transiently innervate cochlear inner hair cells (IHCs). Although acetylcholine (ACh) is the main neurotransmitter at this synapse, an abundant GABA innervation is also present. Electrical stimulation of MOC efferent fibers triggers the release of ACh, but also activates presynaptic GABAB receptors, that in turn reduce the amount of ACh release. GABA-mediated mechanism is through the inhibition of P/Q type Ca2+ channels. We are now studying the consequences of GABAB-mediated inhibition in the short-term plasticity of this synapse. Inhibitory synaptic currents (IPSC) were recorded in IHCs of acutely isolated organs of Corti at P9-P11 while MOC fibers were electrically stimulated. In control condition, 10 pulses applied at high frequency (50 Hz) resulted in a progressive decrease on IPSC amplitudes throughout the train (P10/P1= 0.54). Contrary, specific GABAB agonist, baclofen, increased facilitation rate and eliminated depression at the same frequency (P10/P1= 1). Moreover, application of CGP35348, a GABAB antagonist, produced a bigger depression even at low stimulation frequencies (10Hz). These results suggest that the activation of presynaptic GABAB receptors, minimizes depression and would enable sustained transmission during high-frequency stimulation at the MOC-inner hair cell synapse.
Fil: Wedemeyer, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Katz, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacologia; Argentina
XXXII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Neurociencias
description During development, medial olivocochlear (MOC) neurons transiently innervate cochlear inner hair cells (IHCs). Although acetylcholine (ACh) is the main neurotransmitter at this synapse, an abundant GABA innervation is also present. Electrical stimulation of MOC efferent fibers triggers the release of ACh, but also activates presynaptic GABAB receptors, that in turn reduce the amount of ACh release. GABA-mediated mechanism is through the inhibition of P/Q type Ca2+ channels. We are now studying the consequences of GABAB-mediated inhibition in the short-term plasticity of this synapse. Inhibitory synaptic currents (IPSC) were recorded in IHCs of acutely isolated organs of Corti at P9-P11 while MOC fibers were electrically stimulated. In control condition, 10 pulses applied at high frequency (50 Hz) resulted in a progressive decrease on IPSC amplitudes throughout the train (P10/P1= 0.54). Contrary, specific GABAB agonist, baclofen, increased facilitation rate and eliminated depression at the same frequency (P10/P1= 1). Moreover, application of CGP35348, a GABAB antagonist, produced a bigger depression even at low stimulation frequencies (10Hz). These results suggest that the activation of presynaptic GABAB receptors, minimizes depression and would enable sustained transmission during high-frequency stimulation at the MOC-inner hair cell synapse.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215097
Activation of presynaptic GABAB receptors minimize depression and enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses; XXXII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; Mar del Plata; Argentina; 2017; 1-1
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215097
identifier_str_mv Activation of presynaptic GABAB receptors minimize depression and enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses; XXXII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; Mar del Plata; Argentina; 2017; 1-1
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.saneurociencias.org.ar/san2017-abstract-book/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencia
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencia
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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