Developmental regulation of nicotinic synapses on cochlear inner hair cells

Autores
Katz, E.; Elgoyhen, A.B.; Gómez-Casati, M.E.; Knipper, M.; Vetter, D.E.; Fuchs, P.A.; Glowatzki, E.
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the mature cochlea, inner hair cells (IHCs) transduce acoustic signals into receptor potentials, communicating to the brain by synaptic contacts with afferent fibers. Before the onset of hearing, a transient efferent innervation is found on IHCs, mediated by a nicotinic cholinergic receptor that may contain both α9 and α10 subunits. Calcium influx through that receptor activates calcium-dependent (SK2-containing) potassium channels. This inhibitory synapse is thought to disappear after the onset of hearing [after postnatal day 12 (P12)]. We documented this developmental transition using whole-cell recordings from IHCs in apical turns of the rat organ of Corti. Acetylcholine elicited ionic currents in 88-100% of IHCs between P3 and P14, but in only 1 of 11 IHCs at P16-P22. Potassium depolarization of efferent terminals caused IPSCs in 67% of IHCs at P3, in 100% at P7-P9, in 93% at P10-P12, but in only 40% at P13-P14 and in none of the IHCs tested between P16 and P22. Earlier work had shown by in situ hybridization that α9 mRNA is expressed in adult IHCs but that α10 mRNA disappears after the onset of hearing. In the present study, antibodies to α10 and to the associated calcium-dependent (SK2) potassium channel showed a similar developmental loss. The correlated expression of these gene products with functional innervation suggests that Alpha10 and SK2, but not Alpha9, are regulated by synaptic activity. Furthermore, this developmental knock-out of α10, but not α9, supports the hypothesis that functional nicotinic acetylcholine receptors in hair cells are heteromers containing both these subunits.
Fil:Katz, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Gómez-Casati, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
J. Neurosci. 2004;24(36):7814-7820
Materia
α9α10 nAChR
Ca2+-activated K+ channel
Cholinergic
Efferent innervation
IHC
Mammalian cochlea
Neonatal development
Transient synapse
acetylcholine
calcium
calcium activated potassium channel
messenger RNA
nicotine
nicotinic receptor
nicotinic receptor alpha10 subunit
nicotinic receptor alpha9 subunit
potassium
receptor subunit
unclassified drug
Alpha10 gene
Alpha9 gene
animal cell
animal tissue
article
auditory stimulation
calcium transport
cochlea
Corti organ
depolarization
efferent nerve
gene
hair cell
hearing
in situ hybridization
ion current
knockout gene
newborn
nonhuman
postnatal development
priority journal
protein expression
rat
receptor potential
sensory nerve
signal transduction
sk2 gene
synapse
synaptogenesis
Acetylcholine
Action Potentials
Age Factors
Animals
Cochlea
Gene Expression Regulation, Developmental
Hair Cells, Inner
Hearing
Patch-Clamp Techniques
Potassium
Potassium Channels, Calcium-Activated
Protein Subunits
Rats
Rats, Sprague-Dawley
Receptors, Nicotinic
Small-Conductance Calcium-Activated Potassium Channels
Synaptic Transmission
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_02706474_v24_n36_p7814_Katz

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oai_identifier_str paperaa:paper_02706474_v24_n36_p7814_Katz
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Developmental regulation of nicotinic synapses on cochlear inner hair cellsKatz, E.Elgoyhen, A.B.Gómez-Casati, M.E.Knipper, M.Vetter, D.E.Fuchs, P.A.Glowatzki, E.α9α10 nAChRCa2+-activated K+ channelCholinergicEfferent innervationIHCMammalian cochleaNeonatal developmentTransient synapseacetylcholinecalciumcalcium activated potassium channelmessenger RNAnicotinenicotinic receptornicotinic receptor alpha10 subunitnicotinic receptor alpha9 subunitpotassiumreceptor subunitunclassified drugAlpha10 geneAlpha9 geneanimal cellanimal tissuearticleauditory stimulationcalcium transportcochleaCorti organdepolarizationefferent nervegenehair cellhearingin situ hybridizationion currentknockout genenewbornnonhumanpostnatal developmentpriority journalprotein expressionratreceptor potentialsensory nervesignal transductionsk2 genesynapsesynaptogenesisAcetylcholineAction PotentialsAge FactorsAnimalsCochleaGene Expression Regulation, DevelopmentalHair Cells, InnerHearingPatch-Clamp TechniquesPotassiumPotassium Channels, Calcium-ActivatedProtein SubunitsRatsRats, Sprague-DawleyReceptors, NicotinicSmall-Conductance Calcium-Activated Potassium ChannelsSynaptic TransmissionIn the mature cochlea, inner hair cells (IHCs) transduce acoustic signals into receptor potentials, communicating to the brain by synaptic contacts with afferent fibers. Before the onset of hearing, a transient efferent innervation is found on IHCs, mediated by a nicotinic cholinergic receptor that may contain both α9 and α10 subunits. Calcium influx through that receptor activates calcium-dependent (SK2-containing) potassium channels. This inhibitory synapse is thought to disappear after the onset of hearing [after postnatal day 12 (P12)]. We documented this developmental transition using whole-cell recordings from IHCs in apical turns of the rat organ of Corti. Acetylcholine elicited ionic currents in 88-100% of IHCs between P3 and P14, but in only 1 of 11 IHCs at P16-P22. Potassium depolarization of efferent terminals caused IPSCs in 67% of IHCs at P3, in 100% at P7-P9, in 93% at P10-P12, but in only 40% at P13-P14 and in none of the IHCs tested between P16 and P22. Earlier work had shown by in situ hybridization that α9 mRNA is expressed in adult IHCs but that α10 mRNA disappears after the onset of hearing. In the present study, antibodies to α10 and to the associated calcium-dependent (SK2) potassium channel showed a similar developmental loss. The correlated expression of these gene products with functional innervation suggests that Alpha10 and SK2, but not Alpha9, are regulated by synaptic activity. Furthermore, this developmental knock-out of α10, but not α9, supports the hypothesis that functional nicotinic acetylcholine receptors in hair cells are heteromers containing both these subunits.Fil:Katz, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Gómez-Casati, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2004info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_02706474_v24_n36_p7814_KatzJ. Neurosci. 2004;24(36):7814-7820reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:09Zpaperaa:paper_02706474_v24_n36_p7814_KatzInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:10.586Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Developmental regulation of nicotinic synapses on cochlear inner hair cells
title Developmental regulation of nicotinic synapses on cochlear inner hair cells
spellingShingle Developmental regulation of nicotinic synapses on cochlear inner hair cells
Katz, E.
α9α10 nAChR
Ca2+-activated K+ channel
Cholinergic
Efferent innervation
IHC
Mammalian cochlea
Neonatal development
Transient synapse
acetylcholine
calcium
calcium activated potassium channel
messenger RNA
nicotine
nicotinic receptor
nicotinic receptor alpha10 subunit
nicotinic receptor alpha9 subunit
potassium
receptor subunit
unclassified drug
Alpha10 gene
Alpha9 gene
animal cell
animal tissue
article
auditory stimulation
calcium transport
cochlea
Corti organ
depolarization
efferent nerve
gene
hair cell
hearing
in situ hybridization
ion current
knockout gene
newborn
nonhuman
postnatal development
priority journal
protein expression
rat
receptor potential
sensory nerve
signal transduction
sk2 gene
synapse
synaptogenesis
Acetylcholine
Action Potentials
Age Factors
Animals
Cochlea
Gene Expression Regulation, Developmental
Hair Cells, Inner
Hearing
Patch-Clamp Techniques
Potassium
Potassium Channels, Calcium-Activated
Protein Subunits
Rats
Rats, Sprague-Dawley
Receptors, Nicotinic
Small-Conductance Calcium-Activated Potassium Channels
Synaptic Transmission
title_short Developmental regulation of nicotinic synapses on cochlear inner hair cells
title_full Developmental regulation of nicotinic synapses on cochlear inner hair cells
title_fullStr Developmental regulation of nicotinic synapses on cochlear inner hair cells
title_full_unstemmed Developmental regulation of nicotinic synapses on cochlear inner hair cells
title_sort Developmental regulation of nicotinic synapses on cochlear inner hair cells
dc.creator.none.fl_str_mv Katz, E.
Elgoyhen, A.B.
Gómez-Casati, M.E.
Knipper, M.
Vetter, D.E.
Fuchs, P.A.
Glowatzki, E.
author Katz, E.
author_facet Katz, E.
Elgoyhen, A.B.
Gómez-Casati, M.E.
Knipper, M.
Vetter, D.E.
Fuchs, P.A.
Glowatzki, E.
author_role author
author2 Elgoyhen, A.B.
Gómez-Casati, M.E.
Knipper, M.
Vetter, D.E.
Fuchs, P.A.
Glowatzki, E.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv α9α10 nAChR
Ca2+-activated K+ channel
Cholinergic
Efferent innervation
IHC
Mammalian cochlea
Neonatal development
Transient synapse
acetylcholine
calcium
calcium activated potassium channel
messenger RNA
nicotine
nicotinic receptor
nicotinic receptor alpha10 subunit
nicotinic receptor alpha9 subunit
potassium
receptor subunit
unclassified drug
Alpha10 gene
Alpha9 gene
animal cell
animal tissue
article
auditory stimulation
calcium transport
cochlea
Corti organ
depolarization
efferent nerve
gene
hair cell
hearing
in situ hybridization
ion current
knockout gene
newborn
nonhuman
postnatal development
priority journal
protein expression
rat
receptor potential
sensory nerve
signal transduction
sk2 gene
synapse
synaptogenesis
Acetylcholine
Action Potentials
Age Factors
Animals
Cochlea
Gene Expression Regulation, Developmental
Hair Cells, Inner
Hearing
Patch-Clamp Techniques
Potassium
Potassium Channels, Calcium-Activated
Protein Subunits
Rats
Rats, Sprague-Dawley
Receptors, Nicotinic
Small-Conductance Calcium-Activated Potassium Channels
Synaptic Transmission
topic α9α10 nAChR
Ca2+-activated K+ channel
Cholinergic
Efferent innervation
IHC
Mammalian cochlea
Neonatal development
Transient synapse
acetylcholine
calcium
calcium activated potassium channel
messenger RNA
nicotine
nicotinic receptor
nicotinic receptor alpha10 subunit
nicotinic receptor alpha9 subunit
potassium
receptor subunit
unclassified drug
Alpha10 gene
Alpha9 gene
animal cell
animal tissue
article
auditory stimulation
calcium transport
cochlea
Corti organ
depolarization
efferent nerve
gene
hair cell
hearing
in situ hybridization
ion current
knockout gene
newborn
nonhuman
postnatal development
priority journal
protein expression
rat
receptor potential
sensory nerve
signal transduction
sk2 gene
synapse
synaptogenesis
Acetylcholine
Action Potentials
Age Factors
Animals
Cochlea
Gene Expression Regulation, Developmental
Hair Cells, Inner
Hearing
Patch-Clamp Techniques
Potassium
Potassium Channels, Calcium-Activated
Protein Subunits
Rats
Rats, Sprague-Dawley
Receptors, Nicotinic
Small-Conductance Calcium-Activated Potassium Channels
Synaptic Transmission
dc.description.none.fl_txt_mv In the mature cochlea, inner hair cells (IHCs) transduce acoustic signals into receptor potentials, communicating to the brain by synaptic contacts with afferent fibers. Before the onset of hearing, a transient efferent innervation is found on IHCs, mediated by a nicotinic cholinergic receptor that may contain both α9 and α10 subunits. Calcium influx through that receptor activates calcium-dependent (SK2-containing) potassium channels. This inhibitory synapse is thought to disappear after the onset of hearing [after postnatal day 12 (P12)]. We documented this developmental transition using whole-cell recordings from IHCs in apical turns of the rat organ of Corti. Acetylcholine elicited ionic currents in 88-100% of IHCs between P3 and P14, but in only 1 of 11 IHCs at P16-P22. Potassium depolarization of efferent terminals caused IPSCs in 67% of IHCs at P3, in 100% at P7-P9, in 93% at P10-P12, but in only 40% at P13-P14 and in none of the IHCs tested between P16 and P22. Earlier work had shown by in situ hybridization that α9 mRNA is expressed in adult IHCs but that α10 mRNA disappears after the onset of hearing. In the present study, antibodies to α10 and to the associated calcium-dependent (SK2) potassium channel showed a similar developmental loss. The correlated expression of these gene products with functional innervation suggests that Alpha10 and SK2, but not Alpha9, are regulated by synaptic activity. Furthermore, this developmental knock-out of α10, but not α9, supports the hypothesis that functional nicotinic acetylcholine receptors in hair cells are heteromers containing both these subunits.
Fil:Katz, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Gómez-Casati, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description In the mature cochlea, inner hair cells (IHCs) transduce acoustic signals into receptor potentials, communicating to the brain by synaptic contacts with afferent fibers. Before the onset of hearing, a transient efferent innervation is found on IHCs, mediated by a nicotinic cholinergic receptor that may contain both α9 and α10 subunits. Calcium influx through that receptor activates calcium-dependent (SK2-containing) potassium channels. This inhibitory synapse is thought to disappear after the onset of hearing [after postnatal day 12 (P12)]. We documented this developmental transition using whole-cell recordings from IHCs in apical turns of the rat organ of Corti. Acetylcholine elicited ionic currents in 88-100% of IHCs between P3 and P14, but in only 1 of 11 IHCs at P16-P22. Potassium depolarization of efferent terminals caused IPSCs in 67% of IHCs at P3, in 100% at P7-P9, in 93% at P10-P12, but in only 40% at P13-P14 and in none of the IHCs tested between P16 and P22. Earlier work had shown by in situ hybridization that α9 mRNA is expressed in adult IHCs but that α10 mRNA disappears after the onset of hearing. In the present study, antibodies to α10 and to the associated calcium-dependent (SK2) potassium channel showed a similar developmental loss. The correlated expression of these gene products with functional innervation suggests that Alpha10 and SK2, but not Alpha9, are regulated by synaptic activity. Furthermore, this developmental knock-out of α10, but not α9, supports the hypothesis that functional nicotinic acetylcholine receptors in hair cells are heteromers containing both these subunits.
publishDate 2004
dc.date.none.fl_str_mv 2004
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_02706474_v24_n36_p7814_Katz
url http://hdl.handle.net/20.500.12110/paper_02706474_v24_n36_p7814_Katz
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv J. Neurosci. 2004;24(36):7814-7820
reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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