Effects of single injection of naloxone and damgo within nucleus accumbens septi in the plus maze test in rats

Autores
Morsucci, Cristian; Okasova, Ainura; Mulet, Daniela; Galiana, Graciana; Rodriguez, Vanesa; Baiardi, Gustavo Carlos; Lafuente, José Vicente; Elias, Pablo Adolfo; Landa, Adriana; Soaje, Marta; Gargiulo, Pascual Angel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nucleus accumbens septi (NAS) is studied because its relations with cognition and anxiety. Its pharmacological manipulation is widely used in experimental psychopathology to reproduce psychotic signs and symptoms in animal models. In the present study, the effect of the injection of an agonist and a µ-receptor antagonist in this structure is assessed. Holtzman strain male rats (240-290 g) were cannulated bilaterally in NAS. One week after the injection they were subjected to an anxiety test, prior saline injection (controls), DAMGO ([D-Ala2, N-MePhe4, Gly-ol]-encephalin, opioid agonist) or naloxone (opioid antagonist). We evaluated the set of parameters classically considered in our laboratory (open arm time, time per entry, open arm entries, closed arm entries, open/closed arm quotient, open and closed arm ends arrivals, rearing, fecal bowls and grooming behaviors. There was only a significant increase in the length of stay in the open arm with the injection of DAMGO (0.2 µg/1 µL, p < 0.05) and a significant increase in grooming behaviors with naloxone (1 µg/1 µL, p < 0.001), compared with saline controls (1 µL). We conclude that the receptor stimulation in NAS generates effects compatible with anxiolysis, and blocking of such receptor in said structure results in an increase in grooming behaviors.
Fil: Morsucci C. Universidad Nacional de Cuyo; Argentina
Fil: Okasova A. Universidad Nacional de Cuyo; Argentina
Fil: Mulet, Daniela. Universidad Nacional de Cuyo; Argentina
Fil: Galiana, Graciana. Universidad Nacional de Cuyo; Argentina
Fil: Rodriguez, Vanesa. Universidad Nacional de Cuyo; Argentina
Fil: Baiardi, Gustavo Carlos. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Lafuente, José Vicente. Universidad Nacional de Cuyo; Argentina
Fil: Elias, Pablo Adolfo. Universidad Nacional de Cuyo; Argentina
Fil: Landa, Adriana. Universidad Nacional de Cuyo; Argentina
Fil: Soaje, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Gargiulo, Pascual Angel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
opioides
opiaceos
nucleo accumbens septi (NAS)
Ansiedad
gromming
percepcion
plus maze test
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96101
Acceder
id CONICETDig_665a45a2507ea15a37e48ba0d4c5ff70
oai_identifier_str oai:ri.conicet.gov.ar:11336/96101
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of single injection of naloxone and damgo within nucleus accumbens septi in the plus maze test in ratsMorsucci, CristianOkasova, AinuraMulet, DanielaGaliana, GracianaRodriguez, VanesaBaiardi, Gustavo CarlosLafuente, José VicenteElias, Pablo AdolfoLanda, AdrianaSoaje, MartaGargiulo, Pascual Angelopioidesopiaceosnucleo accumbens septi (NAS)Ansiedadgrommingpercepcionplus maze testhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Nucleus accumbens septi (NAS) is studied because its relations with cognition and anxiety. Its pharmacological manipulation is widely used in experimental psychopathology to reproduce psychotic signs and symptoms in animal models. In the present study, the effect of the injection of an agonist and a µ-receptor antagonist in this structure is assessed. Holtzman strain male rats (240-290 g) were cannulated bilaterally in NAS. One week after the injection they were subjected to an anxiety test, prior saline injection (controls), DAMGO ([D-Ala2, N-MePhe4, Gly-ol]-encephalin, opioid agonist) or naloxone (opioid antagonist). We evaluated the set of parameters classically considered in our laboratory (open arm time, time per entry, open arm entries, closed arm entries, open/closed arm quotient, open and closed arm ends arrivals, rearing, fecal bowls and grooming behaviors. There was only a significant increase in the length of stay in the open arm with the injection of DAMGO (0.2 µg/1 µL, p < 0.05) and a significant increase in grooming behaviors with naloxone (1 µg/1 µL, p < 0.001), compared with saline controls (1 µL). We conclude that the receptor stimulation in NAS generates effects compatible with anxiolysis, and blocking of such receptor in said structure results in an increase in grooming behaviors.Fil: Morsucci C. Universidad Nacional de Cuyo; ArgentinaFil: Okasova A. Universidad Nacional de Cuyo; ArgentinaFil: Mulet, Daniela. Universidad Nacional de Cuyo; ArgentinaFil: Galiana, Graciana. Universidad Nacional de Cuyo; ArgentinaFil: Rodriguez, Vanesa. Universidad Nacional de Cuyo; ArgentinaFil: Baiardi, Gustavo Carlos. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Lafuente, José Vicente. Universidad Nacional de Cuyo; ArgentinaFil: Elias, Pablo Adolfo. Universidad Nacional de Cuyo; ArgentinaFil: Landa, Adriana. Universidad Nacional de Cuyo; ArgentinaFil: Soaje, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Gargiulo, Pascual Angel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaPolskiego Towarzystwa Farmaceutycznego2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96101Morsucci, Cristian; Okasova, Ainura; Mulet, Daniela; Galiana, Graciana; Rodriguez, Vanesa; et al.; Effects of single injection of naloxone and damgo within nucleus accumbens septi in the plus maze test in rats; Polskiego Towarzystwa Farmaceutycznego; Acta Poloniae Pharmaceutica; 75; 1; 1-2018; 205-2100001-6837CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ptfarm.pl/en/wydawnictwa/czasopisma/acta-poloniae-pharmaceutica/110/-/27251info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:05:00Zoai:ri.conicet.gov.ar:11336/96101instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:05:00.644CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of single injection of naloxone and damgo within nucleus accumbens septi in the plus maze test in rats
title Effects of single injection of naloxone and damgo within nucleus accumbens septi in the plus maze test in rats
spellingShingle Effects of single injection of naloxone and damgo within nucleus accumbens septi in the plus maze test in rats
Morsucci, Cristian
opioides
opiaceos
nucleo accumbens septi (NAS)
Ansiedad
gromming
percepcion
plus maze test
title_short Effects of single injection of naloxone and damgo within nucleus accumbens septi in the plus maze test in rats
title_full Effects of single injection of naloxone and damgo within nucleus accumbens septi in the plus maze test in rats
title_fullStr Effects of single injection of naloxone and damgo within nucleus accumbens septi in the plus maze test in rats
title_full_unstemmed Effects of single injection of naloxone and damgo within nucleus accumbens septi in the plus maze test in rats
title_sort Effects of single injection of naloxone and damgo within nucleus accumbens septi in the plus maze test in rats
dc.creator.none.fl_str_mv Morsucci, Cristian
Okasova, Ainura
Mulet, Daniela
Galiana, Graciana
Rodriguez, Vanesa
Baiardi, Gustavo Carlos
Lafuente, José Vicente
Elias, Pablo Adolfo
Landa, Adriana
Soaje, Marta
Gargiulo, Pascual Angel
author Morsucci, Cristian
author_facet Morsucci, Cristian
Okasova, Ainura
Mulet, Daniela
Galiana, Graciana
Rodriguez, Vanesa
Baiardi, Gustavo Carlos
Lafuente, José Vicente
Elias, Pablo Adolfo
Landa, Adriana
Soaje, Marta
Gargiulo, Pascual Angel
author_role author
author2 Okasova, Ainura
Mulet, Daniela
Galiana, Graciana
Rodriguez, Vanesa
Baiardi, Gustavo Carlos
Lafuente, José Vicente
Elias, Pablo Adolfo
Landa, Adriana
Soaje, Marta
Gargiulo, Pascual Angel
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv opioides
opiaceos
nucleo accumbens septi (NAS)
Ansiedad
gromming
percepcion
plus maze test
topic opioides
opiaceos
nucleo accumbens septi (NAS)
Ansiedad
gromming
percepcion
plus maze test
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Nucleus accumbens septi (NAS) is studied because its relations with cognition and anxiety. Its pharmacological manipulation is widely used in experimental psychopathology to reproduce psychotic signs and symptoms in animal models. In the present study, the effect of the injection of an agonist and a µ-receptor antagonist in this structure is assessed. Holtzman strain male rats (240-290 g) were cannulated bilaterally in NAS. One week after the injection they were subjected to an anxiety test, prior saline injection (controls), DAMGO ([D-Ala2, N-MePhe4, Gly-ol]-encephalin, opioid agonist) or naloxone (opioid antagonist). We evaluated the set of parameters classically considered in our laboratory (open arm time, time per entry, open arm entries, closed arm entries, open/closed arm quotient, open and closed arm ends arrivals, rearing, fecal bowls and grooming behaviors. There was only a significant increase in the length of stay in the open arm with the injection of DAMGO (0.2 µg/1 µL, p < 0.05) and a significant increase in grooming behaviors with naloxone (1 µg/1 µL, p < 0.001), compared with saline controls (1 µL). We conclude that the receptor stimulation in NAS generates effects compatible with anxiolysis, and blocking of such receptor in said structure results in an increase in grooming behaviors.
Fil: Morsucci C. Universidad Nacional de Cuyo; Argentina
Fil: Okasova A. Universidad Nacional de Cuyo; Argentina
Fil: Mulet, Daniela. Universidad Nacional de Cuyo; Argentina
Fil: Galiana, Graciana. Universidad Nacional de Cuyo; Argentina
Fil: Rodriguez, Vanesa. Universidad Nacional de Cuyo; Argentina
Fil: Baiardi, Gustavo Carlos. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Lafuente, José Vicente. Universidad Nacional de Cuyo; Argentina
Fil: Elias, Pablo Adolfo. Universidad Nacional de Cuyo; Argentina
Fil: Landa, Adriana. Universidad Nacional de Cuyo; Argentina
Fil: Soaje, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Gargiulo, Pascual Angel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Nucleus accumbens septi (NAS) is studied because its relations with cognition and anxiety. Its pharmacological manipulation is widely used in experimental psychopathology to reproduce psychotic signs and symptoms in animal models. In the present study, the effect of the injection of an agonist and a µ-receptor antagonist in this structure is assessed. Holtzman strain male rats (240-290 g) were cannulated bilaterally in NAS. One week after the injection they were subjected to an anxiety test, prior saline injection (controls), DAMGO ([D-Ala2, N-MePhe4, Gly-ol]-encephalin, opioid agonist) or naloxone (opioid antagonist). We evaluated the set of parameters classically considered in our laboratory (open arm time, time per entry, open arm entries, closed arm entries, open/closed arm quotient, open and closed arm ends arrivals, rearing, fecal bowls and grooming behaviors. There was only a significant increase in the length of stay in the open arm with the injection of DAMGO (0.2 µg/1 µL, p < 0.05) and a significant increase in grooming behaviors with naloxone (1 µg/1 µL, p < 0.001), compared with saline controls (1 µL). We conclude that the receptor stimulation in NAS generates effects compatible with anxiolysis, and blocking of such receptor in said structure results in an increase in grooming behaviors.
publishDate 2018
dc.date.none.fl_str_mv 2018-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96101
Morsucci, Cristian; Okasova, Ainura; Mulet, Daniela; Galiana, Graciana; Rodriguez, Vanesa; et al.; Effects of single injection of naloxone and damgo within nucleus accumbens septi in the plus maze test in rats; Polskiego Towarzystwa Farmaceutycznego; Acta Poloniae Pharmaceutica; 75; 1; 1-2018; 205-210
0001-6837
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96101
identifier_str_mv Morsucci, Cristian; Okasova, Ainura; Mulet, Daniela; Galiana, Graciana; Rodriguez, Vanesa; et al.; Effects of single injection of naloxone and damgo within nucleus accumbens septi in the plus maze test in rats; Polskiego Towarzystwa Farmaceutycznego; Acta Poloniae Pharmaceutica; 75; 1; 1-2018; 205-210
0001-6837
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ptfarm.pl/en/wydawnictwa/czasopisma/acta-poloniae-pharmaceutica/110/-/27251
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Polskiego Towarzystwa Farmaceutycznego
publisher.none.fl_str_mv Polskiego Towarzystwa Farmaceutycznego
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782401479442432
score 12.982451

Publicaciones similares