Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line

Autores
Nikoloff, Noelia; Ponzinibbio, Maria Virginia; Padula, Gisel; de Luca, Julio Cesar; Golijow, Carlos Daniel; Seoane, Analia Isabel
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In human tumor cells, experimental and clinical evidence indicates that some factors involved in signal transductionand cell growth can also modulate the response to chemotherapeutic treatment. The aim of the presentstudy was to investigate the role of folic acid (FA) as a modulator of carboplatin (CBDCA) activity.Genotoxicity and cytotoxicity induced by CBDCA alone and in combination with FA were assessed in culturedHeLa cells. We used comet assay, mitotic index analysis, MTT and NR assays, cytokinesis-block micronucleuscytome assay and annexin V-IP as different cytotoxicity and genotoxicity approaches for human cervical carcinomacell line studies.The results showed that addition of 900 nMFA together with 40.4mMCBDCA enhanced the activity of the platinumcompound,increasing its effect on cell death by nearly 20%, as evidenced by theMTT and NR assays. Moreover,not only higher levels of DNA and chromosomal damage were reached but also the number of necrotic andapoptotic cells were significantly increased when cell cultures were treated with the combined procedure.This situation opens the possibility to explore the use of FA in platinum-based chemotherapy protocols to reducethe platinum doses for patient treatment and decrease the chance of developing the known side effects withoutlosing biological activity.
Fil: Nikoloff, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Ponzinibbio, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: de Luca, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Golijow, Carlos Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Materia
Carboplatin
Folic Acid
Comet Assay
Hela Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48167
Acceder
id CONICETDig_46da9b35fba032f39eba705f911bb47a
oai_identifier_str oai:ri.conicet.gov.ar:11336/48167
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell lineNikoloff, NoeliaPonzinibbio, Maria VirginiaPadula, Giselde Luca, Julio CesarGolijow, Carlos DanielSeoane, Analia IsabelCarboplatinFolic AcidComet AssayHela Cellshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3In human tumor cells, experimental and clinical evidence indicates that some factors involved in signal transductionand cell growth can also modulate the response to chemotherapeutic treatment. The aim of the presentstudy was to investigate the role of folic acid (FA) as a modulator of carboplatin (CBDCA) activity.Genotoxicity and cytotoxicity induced by CBDCA alone and in combination with FA were assessed in culturedHeLa cells. We used comet assay, mitotic index analysis, MTT and NR assays, cytokinesis-block micronucleuscytome assay and annexin V-IP as different cytotoxicity and genotoxicity approaches for human cervical carcinomacell line studies.The results showed that addition of 900 nMFA together with 40.4mMCBDCA enhanced the activity of the platinumcompound,increasing its effect on cell death by nearly 20%, as evidenced by theMTT and NR assays. Moreover,not only higher levels of DNA and chromosomal damage were reached but also the number of necrotic andapoptotic cells were significantly increased when cell cultures were treated with the combined procedure.This situation opens the possibility to explore the use of FA in platinum-based chemotherapy protocols to reducethe platinum doses for patient treatment and decrease the chance of developing the known side effects withoutlosing biological activity.Fil: Nikoloff, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Ponzinibbio, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: de Luca, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Golijow, Carlos Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaPergamon-Elsevier Science Ltd2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48167Nikoloff, Noelia; Ponzinibbio, Maria Virginia; Padula, Gisel; de Luca, Julio Cesar; Golijow, Carlos Daniel; et al.; Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line; Pergamon-Elsevier Science Ltd; Toxicology in Vitro; 37; 10-2016; 142-1470887-2333CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.tiv.2016.09.019info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0887233316301928info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:48Zoai:ri.conicet.gov.ar:11336/48167instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:48.568CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line
title Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line
spellingShingle Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line
Nikoloff, Noelia
Carboplatin
Folic Acid
Comet Assay
Hela Cells
title_short Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line
title_full Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line
title_fullStr Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line
title_full_unstemmed Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line
title_sort Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line
dc.creator.none.fl_str_mv Nikoloff, Noelia
Ponzinibbio, Maria Virginia
Padula, Gisel
de Luca, Julio Cesar
Golijow, Carlos Daniel
Seoane, Analia Isabel
author Nikoloff, Noelia
author_facet Nikoloff, Noelia
Ponzinibbio, Maria Virginia
Padula, Gisel
de Luca, Julio Cesar
Golijow, Carlos Daniel
Seoane, Analia Isabel
author_role author
author2 Ponzinibbio, Maria Virginia
Padula, Gisel
de Luca, Julio Cesar
Golijow, Carlos Daniel
Seoane, Analia Isabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Carboplatin
Folic Acid
Comet Assay
Hela Cells
topic Carboplatin
Folic Acid
Comet Assay
Hela Cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In human tumor cells, experimental and clinical evidence indicates that some factors involved in signal transductionand cell growth can also modulate the response to chemotherapeutic treatment. The aim of the presentstudy was to investigate the role of folic acid (FA) as a modulator of carboplatin (CBDCA) activity.Genotoxicity and cytotoxicity induced by CBDCA alone and in combination with FA were assessed in culturedHeLa cells. We used comet assay, mitotic index analysis, MTT and NR assays, cytokinesis-block micronucleuscytome assay and annexin V-IP as different cytotoxicity and genotoxicity approaches for human cervical carcinomacell line studies.The results showed that addition of 900 nMFA together with 40.4mMCBDCA enhanced the activity of the platinumcompound,increasing its effect on cell death by nearly 20%, as evidenced by theMTT and NR assays. Moreover,not only higher levels of DNA and chromosomal damage were reached but also the number of necrotic andapoptotic cells were significantly increased when cell cultures were treated with the combined procedure.This situation opens the possibility to explore the use of FA in platinum-based chemotherapy protocols to reducethe platinum doses for patient treatment and decrease the chance of developing the known side effects withoutlosing biological activity.
Fil: Nikoloff, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Ponzinibbio, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: de Luca, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Golijow, Carlos Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
description In human tumor cells, experimental and clinical evidence indicates that some factors involved in signal transductionand cell growth can also modulate the response to chemotherapeutic treatment. The aim of the presentstudy was to investigate the role of folic acid (FA) as a modulator of carboplatin (CBDCA) activity.Genotoxicity and cytotoxicity induced by CBDCA alone and in combination with FA were assessed in culturedHeLa cells. We used comet assay, mitotic index analysis, MTT and NR assays, cytokinesis-block micronucleuscytome assay and annexin V-IP as different cytotoxicity and genotoxicity approaches for human cervical carcinomacell line studies.The results showed that addition of 900 nMFA together with 40.4mMCBDCA enhanced the activity of the platinumcompound,increasing its effect on cell death by nearly 20%, as evidenced by theMTT and NR assays. Moreover,not only higher levels of DNA and chromosomal damage were reached but also the number of necrotic andapoptotic cells were significantly increased when cell cultures were treated with the combined procedure.This situation opens the possibility to explore the use of FA in platinum-based chemotherapy protocols to reducethe platinum doses for patient treatment and decrease the chance of developing the known side effects withoutlosing biological activity.
publishDate 2016
dc.date.none.fl_str_mv 2016-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48167
Nikoloff, Noelia; Ponzinibbio, Maria Virginia; Padula, Gisel; de Luca, Julio Cesar; Golijow, Carlos Daniel; et al.; Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line; Pergamon-Elsevier Science Ltd; Toxicology in Vitro; 37; 10-2016; 142-147
0887-2333
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48167
identifier_str_mv Nikoloff, Noelia; Ponzinibbio, Maria Virginia; Padula, Gisel; de Luca, Julio Cesar; Golijow, Carlos Daniel; et al.; Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line; Pergamon-Elsevier Science Ltd; Toxicology in Vitro; 37; 10-2016; 142-147
0887-2333
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tiv.2016.09.019
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0887233316301928
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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