Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line
- Autores
- Nikoloff, Noelia; Ponzinibbio, Maria Virginia; Padula, Gisel; de Luca, Julio Cesar; Golijow, Carlos Daniel; Seoane, Analia Isabel
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In human tumor cells, experimental and clinical evidence indicates that some factors involved in signal transductionand cell growth can also modulate the response to chemotherapeutic treatment. The aim of the presentstudy was to investigate the role of folic acid (FA) as a modulator of carboplatin (CBDCA) activity.Genotoxicity and cytotoxicity induced by CBDCA alone and in combination with FA were assessed in culturedHeLa cells. We used comet assay, mitotic index analysis, MTT and NR assays, cytokinesis-block micronucleuscytome assay and annexin V-IP as different cytotoxicity and genotoxicity approaches for human cervical carcinomacell line studies.The results showed that addition of 900 nMFA together with 40.4mMCBDCA enhanced the activity of the platinumcompound,increasing its effect on cell death by nearly 20%, as evidenced by theMTT and NR assays. Moreover,not only higher levels of DNA and chromosomal damage were reached but also the number of necrotic andapoptotic cells were significantly increased when cell cultures were treated with the combined procedure.This situation opens the possibility to explore the use of FA in platinum-based chemotherapy protocols to reducethe platinum doses for patient treatment and decrease the chance of developing the known side effects withoutlosing biological activity.
Fil: Nikoloff, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Ponzinibbio, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: de Luca, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Golijow, Carlos Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina - Materia
-
Carboplatin
Folic Acid
Comet Assay
Hela Cells - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/48167
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Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell lineNikoloff, NoeliaPonzinibbio, Maria VirginiaPadula, Giselde Luca, Julio CesarGolijow, Carlos DanielSeoane, Analia IsabelCarboplatinFolic AcidComet AssayHela Cellshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3In human tumor cells, experimental and clinical evidence indicates that some factors involved in signal transductionand cell growth can also modulate the response to chemotherapeutic treatment. The aim of the presentstudy was to investigate the role of folic acid (FA) as a modulator of carboplatin (CBDCA) activity.Genotoxicity and cytotoxicity induced by CBDCA alone and in combination with FA were assessed in culturedHeLa cells. We used comet assay, mitotic index analysis, MTT and NR assays, cytokinesis-block micronucleuscytome assay and annexin V-IP as different cytotoxicity and genotoxicity approaches for human cervical carcinomacell line studies.The results showed that addition of 900 nMFA together with 40.4mMCBDCA enhanced the activity of the platinumcompound,increasing its effect on cell death by nearly 20%, as evidenced by theMTT and NR assays. Moreover,not only higher levels of DNA and chromosomal damage were reached but also the number of necrotic andapoptotic cells were significantly increased when cell cultures were treated with the combined procedure.This situation opens the possibility to explore the use of FA in platinum-based chemotherapy protocols to reducethe platinum doses for patient treatment and decrease the chance of developing the known side effects withoutlosing biological activity.Fil: Nikoloff, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Ponzinibbio, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: de Luca, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Golijow, Carlos Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaPergamon-Elsevier Science Ltd2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48167Nikoloff, Noelia; Ponzinibbio, Maria Virginia; Padula, Gisel; de Luca, Julio Cesar; Golijow, Carlos Daniel; et al.; Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line; Pergamon-Elsevier Science Ltd; Toxicology in Vitro; 37; 10-2016; 142-1470887-2333CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.tiv.2016.09.019info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0887233316301928info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:53Zoai:ri.conicet.gov.ar:11336/48167instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:53.667CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line |
title |
Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line |
spellingShingle |
Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line Nikoloff, Noelia Carboplatin Folic Acid Comet Assay Hela Cells |
title_short |
Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line |
title_full |
Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line |
title_fullStr |
Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line |
title_full_unstemmed |
Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line |
title_sort |
Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line |
dc.creator.none.fl_str_mv |
Nikoloff, Noelia Ponzinibbio, Maria Virginia Padula, Gisel de Luca, Julio Cesar Golijow, Carlos Daniel Seoane, Analia Isabel |
author |
Nikoloff, Noelia |
author_facet |
Nikoloff, Noelia Ponzinibbio, Maria Virginia Padula, Gisel de Luca, Julio Cesar Golijow, Carlos Daniel Seoane, Analia Isabel |
author_role |
author |
author2 |
Ponzinibbio, Maria Virginia Padula, Gisel de Luca, Julio Cesar Golijow, Carlos Daniel Seoane, Analia Isabel |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Carboplatin Folic Acid Comet Assay Hela Cells |
topic |
Carboplatin Folic Acid Comet Assay Hela Cells |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
In human tumor cells, experimental and clinical evidence indicates that some factors involved in signal transductionand cell growth can also modulate the response to chemotherapeutic treatment. The aim of the presentstudy was to investigate the role of folic acid (FA) as a modulator of carboplatin (CBDCA) activity.Genotoxicity and cytotoxicity induced by CBDCA alone and in combination with FA were assessed in culturedHeLa cells. We used comet assay, mitotic index analysis, MTT and NR assays, cytokinesis-block micronucleuscytome assay and annexin V-IP as different cytotoxicity and genotoxicity approaches for human cervical carcinomacell line studies.The results showed that addition of 900 nMFA together with 40.4mMCBDCA enhanced the activity of the platinumcompound,increasing its effect on cell death by nearly 20%, as evidenced by theMTT and NR assays. Moreover,not only higher levels of DNA and chromosomal damage were reached but also the number of necrotic andapoptotic cells were significantly increased when cell cultures were treated with the combined procedure.This situation opens the possibility to explore the use of FA in platinum-based chemotherapy protocols to reducethe platinum doses for patient treatment and decrease the chance of developing the known side effects withoutlosing biological activity. Fil: Nikoloff, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina Fil: Ponzinibbio, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina Fil: de Luca, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina Fil: Golijow, Carlos Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina Fil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina |
description |
In human tumor cells, experimental and clinical evidence indicates that some factors involved in signal transductionand cell growth can also modulate the response to chemotherapeutic treatment. The aim of the presentstudy was to investigate the role of folic acid (FA) as a modulator of carboplatin (CBDCA) activity.Genotoxicity and cytotoxicity induced by CBDCA alone and in combination with FA were assessed in culturedHeLa cells. We used comet assay, mitotic index analysis, MTT and NR assays, cytokinesis-block micronucleuscytome assay and annexin V-IP as different cytotoxicity and genotoxicity approaches for human cervical carcinomacell line studies.The results showed that addition of 900 nMFA together with 40.4mMCBDCA enhanced the activity of the platinumcompound,increasing its effect on cell death by nearly 20%, as evidenced by theMTT and NR assays. Moreover,not only higher levels of DNA and chromosomal damage were reached but also the number of necrotic andapoptotic cells were significantly increased when cell cultures were treated with the combined procedure.This situation opens the possibility to explore the use of FA in platinum-based chemotherapy protocols to reducethe platinum doses for patient treatment and decrease the chance of developing the known side effects withoutlosing biological activity. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/48167 Nikoloff, Noelia; Ponzinibbio, Maria Virginia; Padula, Gisel; de Luca, Julio Cesar; Golijow, Carlos Daniel; et al.; Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line; Pergamon-Elsevier Science Ltd; Toxicology in Vitro; 37; 10-2016; 142-147 0887-2333 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/48167 |
identifier_str_mv |
Nikoloff, Noelia; Ponzinibbio, Maria Virginia; Padula, Gisel; de Luca, Julio Cesar; Golijow, Carlos Daniel; et al.; Folic acid enhances the apoptotic and genotoxic activity of carboplatin in HeLa cell line; Pergamon-Elsevier Science Ltd; Toxicology in Vitro; 37; 10-2016; 142-147 0887-2333 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tiv.2016.09.019 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0887233316301928 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269123337256960 |
score |
13.13397 |