Physicochemical and antimicrobial properties of boron-complexed polyglycerol–chitosan dendrimers

Autores
Queiroz, Alvaro Antonio Alencar de; Abraham, Gustavo Abel; Camilo, María Aparecida Pires; Higa, Olga Zazuco; Silva, Gerald Saraiva; Fernández, María del Mar; San Roman, Julio
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A polyglycerol with dendritic structure (PGLD) was synthesized by ring-opening polymerization of deprotonated glycidol using a polyglycerol as core functionality in a step-growth process. Then, PGLD reacted with O-carboxymethylated chitosan to obtain PGLD-chitosan dendrimer (PGLD-Ch). After the reaction of PGLD-Ch with boric acid, there was a marked increase in the bulk viscosity evidencing physically that boron can initiate a charge transfer complex formation, (PGLD-Ch)B. Gel permeation chromatography analysis was used to characterize the molecular weight and the polydispersivity of the synthesized PGLD-Ch. A dendritic structure with a molecular mass of 16.7 kDa and a narrow polydispersity (M w/M n = 1.05) was obtained. 1H-NMR and 13C-NMR measurements were employed to assess the degree of branching in PGLD. The obtained value of 0.85 indicates the tendency toward a dentritic structure for PGLD. The glass transition temperature values of (PGLD-Ch)B membranes containing 10% and 30% PGLD were −19°C and −26°C, respectively, which favor its potential use as surface coating of several polymers. The in vitro cytotoxicity was evaluated using the minimum essential medium elution test assay. Extracts of boroncomplexed PGLD exhibited lower cytotoxicity than the controls, suggesting that the material has an improved biocompatibility. Antibacterial studies of (PGLD-Ch)B against Staphylococcus aureus and Pseudomonas aeruginosa showed a significant activity. Our study confirms and supports the effectiveness of (PGLD-Ch)B as an antimicrobial coating due to its capacity in suppressing the bacterial proliferation. The best in vivo response was found for (PGLD-Ch)B-30 membranes, which exhibited higher synthesis of collagen fibers than PGLD-ChB-10.
Fil: Queiroz, Alvaro Antonio Alencar de. Universidade Federal de Itajubá; Brasil
Fil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Camilo, María Aparecida Pires. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energeticas e Nucleares.; Brasil
Fil: Higa, Olga Zazuco. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energeticas e Nucleares.; Brasil
Fil: Silva, Gerald Saraiva. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energeticas e Nucleares.; Brasil
Fil: Fernández, María del Mar. Instituto en Ciencia y Tecnología de Polímeros; España
Fil: San Roman, Julio. Instituto en Ciencia y Tecnología de Polímeros; España
Materia
Polyglycerol
Chitosan
Dendrimers
Antimicrobial Properties
Wound Healing
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41701
Acceder
id CONICETDig_653c744c367406741d59a72a72b1ea43
oai_identifier_str oai:ri.conicet.gov.ar:11336/41701
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Physicochemical and antimicrobial properties of boron-complexed polyglycerol–chitosan dendrimersQueiroz, Alvaro Antonio Alencar deAbraham, Gustavo AbelCamilo, María Aparecida PiresHiga, Olga ZazucoSilva, Gerald SaraivaFernández, María del MarSan Roman, JulioPolyglycerolChitosanDendrimersAntimicrobial PropertiesWound Healinghttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3https://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2A polyglycerol with dendritic structure (PGLD) was synthesized by ring-opening polymerization of deprotonated glycidol using a polyglycerol as core functionality in a step-growth process. Then, PGLD reacted with O-carboxymethylated chitosan to obtain PGLD-chitosan dendrimer (PGLD-Ch). After the reaction of PGLD-Ch with boric acid, there was a marked increase in the bulk viscosity evidencing physically that boron can initiate a charge transfer complex formation, (PGLD-Ch)B. Gel permeation chromatography analysis was used to characterize the molecular weight and the polydispersivity of the synthesized PGLD-Ch. A dendritic structure with a molecular mass of 16.7 kDa and a narrow polydispersity (M w/M n = 1.05) was obtained. 1H-NMR and 13C-NMR measurements were employed to assess the degree of branching in PGLD. The obtained value of 0.85 indicates the tendency toward a dentritic structure for PGLD. The glass transition temperature values of (PGLD-Ch)B membranes containing 10% and 30% PGLD were −19°C and −26°C, respectively, which favor its potential use as surface coating of several polymers. The in vitro cytotoxicity was evaluated using the minimum essential medium elution test assay. Extracts of boroncomplexed PGLD exhibited lower cytotoxicity than the controls, suggesting that the material has an improved biocompatibility. Antibacterial studies of (PGLD-Ch)B against Staphylococcus aureus and Pseudomonas aeruginosa showed a significant activity. Our study confirms and supports the effectiveness of (PGLD-Ch)B as an antimicrobial coating due to its capacity in suppressing the bacterial proliferation. The best in vivo response was found for (PGLD-Ch)B-30 membranes, which exhibited higher synthesis of collagen fibers than PGLD-ChB-10.Fil: Queiroz, Alvaro Antonio Alencar de. Universidade Federal de Itajubá; BrasilFil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Camilo, María Aparecida Pires. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energeticas e Nucleares.; BrasilFil: Higa, Olga Zazuco. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energeticas e Nucleares.; BrasilFil: Silva, Gerald Saraiva. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energeticas e Nucleares.; BrasilFil: Fernández, María del Mar. Instituto en Ciencia y Tecnología de Polímeros; EspañaFil: San Roman, Julio. Instituto en Ciencia y Tecnología de Polímeros; EspañaVsp Bv2006-01-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41701Queiroz, Alvaro Antonio Alencar de; Abraham, Gustavo Abel; Camilo, María Aparecida Pires; Higa, Olga Zazuco; Silva, Gerald Saraiva; et al.; Physicochemical and antimicrobial properties of boron-complexed polyglycerol–chitosan dendrimers; Vsp Bv; Journal of Biomaterials Science - Polymer Edition; 17; 6; 3-1-2006; 689-7070920-5063CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1163/156856206777346313info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1163/156856206777346313info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:06:29Zoai:ri.conicet.gov.ar:11336/41701instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:06:29.557CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Physicochemical and antimicrobial properties of boron-complexed polyglycerol–chitosan dendrimers
title Physicochemical and antimicrobial properties of boron-complexed polyglycerol–chitosan dendrimers
spellingShingle Physicochemical and antimicrobial properties of boron-complexed polyglycerol–chitosan dendrimers
Queiroz, Alvaro Antonio Alencar de
Polyglycerol
Chitosan
Dendrimers
Antimicrobial Properties
Wound Healing
title_short Physicochemical and antimicrobial properties of boron-complexed polyglycerol–chitosan dendrimers
title_full Physicochemical and antimicrobial properties of boron-complexed polyglycerol–chitosan dendrimers
title_fullStr Physicochemical and antimicrobial properties of boron-complexed polyglycerol–chitosan dendrimers
title_full_unstemmed Physicochemical and antimicrobial properties of boron-complexed polyglycerol–chitosan dendrimers
title_sort Physicochemical and antimicrobial properties of boron-complexed polyglycerol–chitosan dendrimers
dc.creator.none.fl_str_mv Queiroz, Alvaro Antonio Alencar de
Abraham, Gustavo Abel
Camilo, María Aparecida Pires
Higa, Olga Zazuco
Silva, Gerald Saraiva
Fernández, María del Mar
San Roman, Julio
author Queiroz, Alvaro Antonio Alencar de
author_facet Queiroz, Alvaro Antonio Alencar de
Abraham, Gustavo Abel
Camilo, María Aparecida Pires
Higa, Olga Zazuco
Silva, Gerald Saraiva
Fernández, María del Mar
San Roman, Julio
author_role author
author2 Abraham, Gustavo Abel
Camilo, María Aparecida Pires
Higa, Olga Zazuco
Silva, Gerald Saraiva
Fernández, María del Mar
San Roman, Julio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Polyglycerol
Chitosan
Dendrimers
Antimicrobial Properties
Wound Healing
topic Polyglycerol
Chitosan
Dendrimers
Antimicrobial Properties
Wound Healing
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv A polyglycerol with dendritic structure (PGLD) was synthesized by ring-opening polymerization of deprotonated glycidol using a polyglycerol as core functionality in a step-growth process. Then, PGLD reacted with O-carboxymethylated chitosan to obtain PGLD-chitosan dendrimer (PGLD-Ch). After the reaction of PGLD-Ch with boric acid, there was a marked increase in the bulk viscosity evidencing physically that boron can initiate a charge transfer complex formation, (PGLD-Ch)B. Gel permeation chromatography analysis was used to characterize the molecular weight and the polydispersivity of the synthesized PGLD-Ch. A dendritic structure with a molecular mass of 16.7 kDa and a narrow polydispersity (M w/M n = 1.05) was obtained. 1H-NMR and 13C-NMR measurements were employed to assess the degree of branching in PGLD. The obtained value of 0.85 indicates the tendency toward a dentritic structure for PGLD. The glass transition temperature values of (PGLD-Ch)B membranes containing 10% and 30% PGLD were −19°C and −26°C, respectively, which favor its potential use as surface coating of several polymers. The in vitro cytotoxicity was evaluated using the minimum essential medium elution test assay. Extracts of boroncomplexed PGLD exhibited lower cytotoxicity than the controls, suggesting that the material has an improved biocompatibility. Antibacterial studies of (PGLD-Ch)B against Staphylococcus aureus and Pseudomonas aeruginosa showed a significant activity. Our study confirms and supports the effectiveness of (PGLD-Ch)B as an antimicrobial coating due to its capacity in suppressing the bacterial proliferation. The best in vivo response was found for (PGLD-Ch)B-30 membranes, which exhibited higher synthesis of collagen fibers than PGLD-ChB-10.
Fil: Queiroz, Alvaro Antonio Alencar de. Universidade Federal de Itajubá; Brasil
Fil: Abraham, Gustavo Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Camilo, María Aparecida Pires. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energeticas e Nucleares.; Brasil
Fil: Higa, Olga Zazuco. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energeticas e Nucleares.; Brasil
Fil: Silva, Gerald Saraiva. Comissao Nacional de Energia Nuclear. Centro de Lasers e Aplicacoes. Instituto de Pesquisas Energeticas e Nucleares.; Brasil
Fil: Fernández, María del Mar. Instituto en Ciencia y Tecnología de Polímeros; España
Fil: San Roman, Julio. Instituto en Ciencia y Tecnología de Polímeros; España
description A polyglycerol with dendritic structure (PGLD) was synthesized by ring-opening polymerization of deprotonated glycidol using a polyglycerol as core functionality in a step-growth process. Then, PGLD reacted with O-carboxymethylated chitosan to obtain PGLD-chitosan dendrimer (PGLD-Ch). After the reaction of PGLD-Ch with boric acid, there was a marked increase in the bulk viscosity evidencing physically that boron can initiate a charge transfer complex formation, (PGLD-Ch)B. Gel permeation chromatography analysis was used to characterize the molecular weight and the polydispersivity of the synthesized PGLD-Ch. A dendritic structure with a molecular mass of 16.7 kDa and a narrow polydispersity (M w/M n = 1.05) was obtained. 1H-NMR and 13C-NMR measurements were employed to assess the degree of branching in PGLD. The obtained value of 0.85 indicates the tendency toward a dentritic structure for PGLD. The glass transition temperature values of (PGLD-Ch)B membranes containing 10% and 30% PGLD were −19°C and −26°C, respectively, which favor its potential use as surface coating of several polymers. The in vitro cytotoxicity was evaluated using the minimum essential medium elution test assay. Extracts of boroncomplexed PGLD exhibited lower cytotoxicity than the controls, suggesting that the material has an improved biocompatibility. Antibacterial studies of (PGLD-Ch)B against Staphylococcus aureus and Pseudomonas aeruginosa showed a significant activity. Our study confirms and supports the effectiveness of (PGLD-Ch)B as an antimicrobial coating due to its capacity in suppressing the bacterial proliferation. The best in vivo response was found for (PGLD-Ch)B-30 membranes, which exhibited higher synthesis of collagen fibers than PGLD-ChB-10.
publishDate 2006
dc.date.none.fl_str_mv 2006-01-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41701
Queiroz, Alvaro Antonio Alencar de; Abraham, Gustavo Abel; Camilo, María Aparecida Pires; Higa, Olga Zazuco; Silva, Gerald Saraiva; et al.; Physicochemical and antimicrobial properties of boron-complexed polyglycerol–chitosan dendrimers; Vsp Bv; Journal of Biomaterials Science - Polymer Edition; 17; 6; 3-1-2006; 689-707
0920-5063
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41701
identifier_str_mv Queiroz, Alvaro Antonio Alencar de; Abraham, Gustavo Abel; Camilo, María Aparecida Pires; Higa, Olga Zazuco; Silva, Gerald Saraiva; et al.; Physicochemical and antimicrobial properties of boron-complexed polyglycerol–chitosan dendrimers; Vsp Bv; Journal of Biomaterials Science - Polymer Edition; 17; 6; 3-1-2006; 689-707
0920-5063
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1163/156856206777346313
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1163/156856206777346313
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Vsp Bv
publisher.none.fl_str_mv Vsp Bv
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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