Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting
- Autores
- Alves Fernandes, Alan Carlos; Pereira de Oliveira, Franciane; Fernandez, Gimena; da Guia Vieira, Luane; Gugelmin Rosa, Cristiane; do Nascimento, Taís; de Castro França, Suzelei; Donato Jr, Jose; Vella, Kristen R.; Antunes Rodrigues, Jose; Mecawi , André; Perello, Mario; Leico Kagohara Elias, Lucila; Rorato, Rodrigo
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background: The activation of the hypothalamic–pituitary–adrenal (HPA) axis is essential for metabolic adaptation in response to fasting. However, the neurocircuitry connecting changes in the peripheral energy stores to the activity of hypothalamic paraventricular corticotrophin-releasing factor (CRFPVN) neurons, the master controller of the HPA axis activity, is not completely understood. Our main goal was to determine if hypothalamic arcuate nucleus (ARC) POMC and AgRP neurons can communicate fasting-induced changes in peripheral energy stores, associated to a fall in plasma leptin levels, to CRFPVN neurons to modulate the HPA axis activity in mice. Results: We observed increased plasma corticosterone levels associate with increased CRFPVN mRNA expression and increased CRFPVN neuronal activity in 36 h fasted mice. These responses were associated with a fall in plasma leptin levels and changes in the mRNA expression of Agrp and Pomc in the ARC. Fasting-induced decrease in plasma leptin partially modulated these responses through a change in the activity of ARC neurons. The chemogenetic activation of POMCARC by DREADDs did not affect fasting-induced activation of the HPA axis. DREADDs inhibition of AgRPARC neurons reduced the content of CRFPVN and increased its accumulation in the median eminence but had no effect on corticosterone secretion induced by fasting. Conclusion: Our data indicate that AgRPARC neurons are part of the neurocircuitry involved in the coupling of PVNCRF activity to changes in peripheral energy stores induced by prolonged fasting.
Fil: Alves Fernandes, Alan Carlos. University Of Ribeirao Preto; Brasil
Fil: Pereira de Oliveira, Franciane. Universidade Federal de Sao Paulo.; Brasil
Fil: Fernandez, Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: da Guia Vieira, Luane. University Of Ribeirao Preto; Brasil
Fil: Gugelmin Rosa, Cristiane. University Of Ribeirao Preto; Brasil
Fil: do Nascimento, Taís. University Of Ribeirao Preto; Brasil
Fil: de Castro França, Suzelei. University Of Ribeirao Preto; Brasil
Fil: Donato Jr, Jose. Universidade de Sao Paulo. Departamento de Fisiología; Brasil
Fil: Vella, Kristen R.. Weill Cornell Medical College; Estados Unidos
Fil: Antunes Rodrigues, Jose. University Of Ribeirao Preto; Brasil
Fil: Mecawi , André. Universidade Federal de Sao Paulo.; Brasil
Fil: Perello, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Leico Kagohara Elias, Lucila. University Of Ribeirao Preto; Brasil
Fil: Rorato, Rodrigo. Universidade Federal de Sao Paulo.; Brasil - Materia
-
AGRP
CRF
DREADD
FASTING
HPA AXIS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/213177
Ver los metadatos del registro completo
| id |
CONICETDig_64c8f18e34a1ec187642141bc22ab42a |
|---|---|
| oai_identifier_str |
oai:ri.conicet.gov.ar:11336/213177 |
| network_acronym_str |
CONICETDig |
| repository_id_str |
3498 |
| network_name_str |
CONICET Digital (CONICET) |
| spelling |
Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fastingAlves Fernandes, Alan CarlosPereira de Oliveira, FrancianeFernandez, Gimenada Guia Vieira, LuaneGugelmin Rosa, Cristianedo Nascimento, Taísde Castro França, SuzeleiDonato Jr, JoseVella, Kristen R.Antunes Rodrigues, JoseMecawi , AndréPerello, MarioLeico Kagohara Elias, LucilaRorato, RodrigoAGRPCRFDREADDFASTINGHPA AXIShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: The activation of the hypothalamic–pituitary–adrenal (HPA) axis is essential for metabolic adaptation in response to fasting. However, the neurocircuitry connecting changes in the peripheral energy stores to the activity of hypothalamic paraventricular corticotrophin-releasing factor (CRFPVN) neurons, the master controller of the HPA axis activity, is not completely understood. Our main goal was to determine if hypothalamic arcuate nucleus (ARC) POMC and AgRP neurons can communicate fasting-induced changes in peripheral energy stores, associated to a fall in plasma leptin levels, to CRFPVN neurons to modulate the HPA axis activity in mice. Results: We observed increased plasma corticosterone levels associate with increased CRFPVN mRNA expression and increased CRFPVN neuronal activity in 36 h fasted mice. These responses were associated with a fall in plasma leptin levels and changes in the mRNA expression of Agrp and Pomc in the ARC. Fasting-induced decrease in plasma leptin partially modulated these responses through a change in the activity of ARC neurons. The chemogenetic activation of POMCARC by DREADDs did not affect fasting-induced activation of the HPA axis. DREADDs inhibition of AgRPARC neurons reduced the content of CRFPVN and increased its accumulation in the median eminence but had no effect on corticosterone secretion induced by fasting. Conclusion: Our data indicate that AgRPARC neurons are part of the neurocircuitry involved in the coupling of PVNCRF activity to changes in peripheral energy stores induced by prolonged fasting.Fil: Alves Fernandes, Alan Carlos. University Of Ribeirao Preto; BrasilFil: Pereira de Oliveira, Franciane. Universidade Federal de Sao Paulo.; BrasilFil: Fernandez, Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: da Guia Vieira, Luane. University Of Ribeirao Preto; BrasilFil: Gugelmin Rosa, Cristiane. University Of Ribeirao Preto; BrasilFil: do Nascimento, Taís. University Of Ribeirao Preto; BrasilFil: de Castro França, Suzelei. University Of Ribeirao Preto; BrasilFil: Donato Jr, Jose. Universidade de Sao Paulo. Departamento de Fisiología; BrasilFil: Vella, Kristen R.. Weill Cornell Medical College; Estados UnidosFil: Antunes Rodrigues, Jose. University Of Ribeirao Preto; BrasilFil: Mecawi , André. Universidade Federal de Sao Paulo.; BrasilFil: Perello, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Leico Kagohara Elias, Lucila. University Of Ribeirao Preto; BrasilFil: Rorato, Rodrigo. Universidade Federal de Sao Paulo.; BrasilBioMed Central2022-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213177Alves Fernandes, Alan Carlos; Pereira de Oliveira, Franciane; Fernandez, Gimena; da Guia Vieira, Luane; Gugelmin Rosa, Cristiane; et al.; Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting; BioMed Central; Cell and Bioscience; 12; 1; 7-2022; 1-142045-3701CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s13578-022-00853-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:45Zoai:ri.conicet.gov.ar:11336/213177instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:45.544CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting |
| title |
Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting |
| spellingShingle |
Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting Alves Fernandes, Alan Carlos AGRP CRF DREADD FASTING HPA AXIS |
| title_short |
Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting |
| title_full |
Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting |
| title_fullStr |
Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting |
| title_full_unstemmed |
Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting |
| title_sort |
Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting |
| dc.creator.none.fl_str_mv |
Alves Fernandes, Alan Carlos Pereira de Oliveira, Franciane Fernandez, Gimena da Guia Vieira, Luane Gugelmin Rosa, Cristiane do Nascimento, Taís de Castro França, Suzelei Donato Jr, Jose Vella, Kristen R. Antunes Rodrigues, Jose Mecawi , André Perello, Mario Leico Kagohara Elias, Lucila Rorato, Rodrigo |
| author |
Alves Fernandes, Alan Carlos |
| author_facet |
Alves Fernandes, Alan Carlos Pereira de Oliveira, Franciane Fernandez, Gimena da Guia Vieira, Luane Gugelmin Rosa, Cristiane do Nascimento, Taís de Castro França, Suzelei Donato Jr, Jose Vella, Kristen R. Antunes Rodrigues, Jose Mecawi , André Perello, Mario Leico Kagohara Elias, Lucila Rorato, Rodrigo |
| author_role |
author |
| author2 |
Pereira de Oliveira, Franciane Fernandez, Gimena da Guia Vieira, Luane Gugelmin Rosa, Cristiane do Nascimento, Taís de Castro França, Suzelei Donato Jr, Jose Vella, Kristen R. Antunes Rodrigues, Jose Mecawi , André Perello, Mario Leico Kagohara Elias, Lucila Rorato, Rodrigo |
| author2_role |
author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
AGRP CRF DREADD FASTING HPA AXIS |
| topic |
AGRP CRF DREADD FASTING HPA AXIS |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| dc.description.none.fl_txt_mv |
Background: The activation of the hypothalamic–pituitary–adrenal (HPA) axis is essential for metabolic adaptation in response to fasting. However, the neurocircuitry connecting changes in the peripheral energy stores to the activity of hypothalamic paraventricular corticotrophin-releasing factor (CRFPVN) neurons, the master controller of the HPA axis activity, is not completely understood. Our main goal was to determine if hypothalamic arcuate nucleus (ARC) POMC and AgRP neurons can communicate fasting-induced changes in peripheral energy stores, associated to a fall in plasma leptin levels, to CRFPVN neurons to modulate the HPA axis activity in mice. Results: We observed increased plasma corticosterone levels associate with increased CRFPVN mRNA expression and increased CRFPVN neuronal activity in 36 h fasted mice. These responses were associated with a fall in plasma leptin levels and changes in the mRNA expression of Agrp and Pomc in the ARC. Fasting-induced decrease in plasma leptin partially modulated these responses through a change in the activity of ARC neurons. The chemogenetic activation of POMCARC by DREADDs did not affect fasting-induced activation of the HPA axis. DREADDs inhibition of AgRPARC neurons reduced the content of CRFPVN and increased its accumulation in the median eminence but had no effect on corticosterone secretion induced by fasting. Conclusion: Our data indicate that AgRPARC neurons are part of the neurocircuitry involved in the coupling of PVNCRF activity to changes in peripheral energy stores induced by prolonged fasting. Fil: Alves Fernandes, Alan Carlos. University Of Ribeirao Preto; Brasil Fil: Pereira de Oliveira, Franciane. Universidade Federal de Sao Paulo.; Brasil Fil: Fernandez, Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina Fil: da Guia Vieira, Luane. University Of Ribeirao Preto; Brasil Fil: Gugelmin Rosa, Cristiane. University Of Ribeirao Preto; Brasil Fil: do Nascimento, Taís. University Of Ribeirao Preto; Brasil Fil: de Castro França, Suzelei. University Of Ribeirao Preto; Brasil Fil: Donato Jr, Jose. Universidade de Sao Paulo. Departamento de Fisiología; Brasil Fil: Vella, Kristen R.. Weill Cornell Medical College; Estados Unidos Fil: Antunes Rodrigues, Jose. University Of Ribeirao Preto; Brasil Fil: Mecawi , André. Universidade Federal de Sao Paulo.; Brasil Fil: Perello, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina Fil: Leico Kagohara Elias, Lucila. University Of Ribeirao Preto; Brasil Fil: Rorato, Rodrigo. Universidade Federal de Sao Paulo.; Brasil |
| description |
Background: The activation of the hypothalamic–pituitary–adrenal (HPA) axis is essential for metabolic adaptation in response to fasting. However, the neurocircuitry connecting changes in the peripheral energy stores to the activity of hypothalamic paraventricular corticotrophin-releasing factor (CRFPVN) neurons, the master controller of the HPA axis activity, is not completely understood. Our main goal was to determine if hypothalamic arcuate nucleus (ARC) POMC and AgRP neurons can communicate fasting-induced changes in peripheral energy stores, associated to a fall in plasma leptin levels, to CRFPVN neurons to modulate the HPA axis activity in mice. Results: We observed increased plasma corticosterone levels associate with increased CRFPVN mRNA expression and increased CRFPVN neuronal activity in 36 h fasted mice. These responses were associated with a fall in plasma leptin levels and changes in the mRNA expression of Agrp and Pomc in the ARC. Fasting-induced decrease in plasma leptin partially modulated these responses through a change in the activity of ARC neurons. The chemogenetic activation of POMCARC by DREADDs did not affect fasting-induced activation of the HPA axis. DREADDs inhibition of AgRPARC neurons reduced the content of CRFPVN and increased its accumulation in the median eminence but had no effect on corticosterone secretion induced by fasting. Conclusion: Our data indicate that AgRPARC neurons are part of the neurocircuitry involved in the coupling of PVNCRF activity to changes in peripheral energy stores induced by prolonged fasting. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-07 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/213177 Alves Fernandes, Alan Carlos; Pereira de Oliveira, Franciane; Fernandez, Gimena; da Guia Vieira, Luane; Gugelmin Rosa, Cristiane; et al.; Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting; BioMed Central; Cell and Bioscience; 12; 1; 7-2022; 1-14 2045-3701 CONICET Digital CONICET |
| url |
http://hdl.handle.net/11336/213177 |
| identifier_str_mv |
Alves Fernandes, Alan Carlos; Pereira de Oliveira, Franciane; Fernandez, Gimena; da Guia Vieira, Luane; Gugelmin Rosa, Cristiane; et al.; Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting; BioMed Central; Cell and Bioscience; 12; 1; 7-2022; 1-14 2045-3701 CONICET Digital CONICET |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13578-022-00853-z |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
| dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
BioMed Central |
| publisher.none.fl_str_mv |
BioMed Central |
| dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
| reponame_str |
CONICET Digital (CONICET) |
| collection |
CONICET Digital (CONICET) |
| instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
| repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
| repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
| _version_ |
1844613941668347904 |
| score |
13.070432 |