Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting

Autores
Alves Fernandes, Alan Carlos; Pereira de Oliveira, Franciane; Fernandez, Gimena; da Guia Vieira, Luane; Gugelmin Rosa, Cristiane; do Nascimento, Taís; de Castro França, Suzelei; Donato Jr, Jose; Vella, Kristen R.; Antunes Rodrigues, Jose; Mecawi , André; Perello, Mario; Leico Kagohara Elias, Lucila; Rorato, Rodrigo
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: The activation of the hypothalamic–pituitary–adrenal (HPA) axis is essential for metabolic adaptation in response to fasting. However, the neurocircuitry connecting changes in the peripheral energy stores to the activity of hypothalamic paraventricular corticotrophin-releasing factor (CRFPVN) neurons, the master controller of the HPA axis activity, is not completely understood. Our main goal was to determine if hypothalamic arcuate nucleus (ARC) POMC and AgRP neurons can communicate fasting-induced changes in peripheral energy stores, associated to a fall in plasma leptin levels, to CRFPVN neurons to modulate the HPA axis activity in mice. Results: We observed increased plasma corticosterone levels associate with increased CRFPVN mRNA expression and increased CRFPVN neuronal activity in 36 h fasted mice. These responses were associated with a fall in plasma leptin levels and changes in the mRNA expression of Agrp and Pomc in the ARC. Fasting-induced decrease in plasma leptin partially modulated these responses through a change in the activity of ARC neurons. The chemogenetic activation of POMCARC by DREADDs did not affect fasting-induced activation of the HPA axis. DREADDs inhibition of AgRPARC neurons reduced the content of CRFPVN and increased its accumulation in the median eminence but had no effect on corticosterone secretion induced by fasting. Conclusion: Our data indicate that AgRPARC neurons are part of the neurocircuitry involved in the coupling of PVNCRF activity to changes in peripheral energy stores induced by prolonged fasting.
Fil: Alves Fernandes, Alan Carlos. University Of Ribeirao Preto; Brasil
Fil: Pereira de Oliveira, Franciane. Universidade Federal de Sao Paulo.; Brasil
Fil: Fernandez, Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: da Guia Vieira, Luane. University Of Ribeirao Preto; Brasil
Fil: Gugelmin Rosa, Cristiane. University Of Ribeirao Preto; Brasil
Fil: do Nascimento, Taís. University Of Ribeirao Preto; Brasil
Fil: de Castro França, Suzelei. University Of Ribeirao Preto; Brasil
Fil: Donato Jr, Jose. Universidade de Sao Paulo. Departamento de Fisiología; Brasil
Fil: Vella, Kristen R.. Weill Cornell Medical College; Estados Unidos
Fil: Antunes Rodrigues, Jose. University Of Ribeirao Preto; Brasil
Fil: Mecawi , André. Universidade Federal de Sao Paulo.; Brasil
Fil: Perello, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Leico Kagohara Elias, Lucila. University Of Ribeirao Preto; Brasil
Fil: Rorato, Rodrigo. Universidade Federal de Sao Paulo.; Brasil
Materia
AGRP
CRF
DREADD
FASTING
HPA AXIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/213177
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/213177
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fastingAlves Fernandes, Alan CarlosPereira de Oliveira, FrancianeFernandez, Gimenada Guia Vieira, LuaneGugelmin Rosa, Cristianedo Nascimento, Taísde Castro França, SuzeleiDonato Jr, JoseVella, Kristen R.Antunes Rodrigues, JoseMecawi , AndréPerello, MarioLeico Kagohara Elias, LucilaRorato, RodrigoAGRPCRFDREADDFASTINGHPA AXIShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: The activation of the hypothalamic–pituitary–adrenal (HPA) axis is essential for metabolic adaptation in response to fasting. However, the neurocircuitry connecting changes in the peripheral energy stores to the activity of hypothalamic paraventricular corticotrophin-releasing factor (CRFPVN) neurons, the master controller of the HPA axis activity, is not completely understood. Our main goal was to determine if hypothalamic arcuate nucleus (ARC) POMC and AgRP neurons can communicate fasting-induced changes in peripheral energy stores, associated to a fall in plasma leptin levels, to CRFPVN neurons to modulate the HPA axis activity in mice. Results: We observed increased plasma corticosterone levels associate with increased CRFPVN mRNA expression and increased CRFPVN neuronal activity in 36 h fasted mice. These responses were associated with a fall in plasma leptin levels and changes in the mRNA expression of Agrp and Pomc in the ARC. Fasting-induced decrease in plasma leptin partially modulated these responses through a change in the activity of ARC neurons. The chemogenetic activation of POMCARC by DREADDs did not affect fasting-induced activation of the HPA axis. DREADDs inhibition of AgRPARC neurons reduced the content of CRFPVN and increased its accumulation in the median eminence but had no effect on corticosterone secretion induced by fasting. Conclusion: Our data indicate that AgRPARC neurons are part of the neurocircuitry involved in the coupling of PVNCRF activity to changes in peripheral energy stores induced by prolonged fasting.Fil: Alves Fernandes, Alan Carlos. University Of Ribeirao Preto; BrasilFil: Pereira de Oliveira, Franciane. Universidade Federal de Sao Paulo.; BrasilFil: Fernandez, Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: da Guia Vieira, Luane. University Of Ribeirao Preto; BrasilFil: Gugelmin Rosa, Cristiane. University Of Ribeirao Preto; BrasilFil: do Nascimento, Taís. University Of Ribeirao Preto; BrasilFil: de Castro França, Suzelei. University Of Ribeirao Preto; BrasilFil: Donato Jr, Jose. Universidade de Sao Paulo. Departamento de Fisiología; BrasilFil: Vella, Kristen R.. Weill Cornell Medical College; Estados UnidosFil: Antunes Rodrigues, Jose. University Of Ribeirao Preto; BrasilFil: Mecawi , André. Universidade Federal de Sao Paulo.; BrasilFil: Perello, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Leico Kagohara Elias, Lucila. University Of Ribeirao Preto; BrasilFil: Rorato, Rodrigo. Universidade Federal de Sao Paulo.; BrasilBioMed Central2022-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213177Alves Fernandes, Alan Carlos; Pereira de Oliveira, Franciane; Fernandez, Gimena; da Guia Vieira, Luane; Gugelmin Rosa, Cristiane; et al.; Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting; BioMed Central; Cell and Bioscience; 12; 1; 7-2022; 1-142045-3701CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/s13578-022-00853-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:00Zoai:ri.conicet.gov.ar:11336/213177instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:00.562CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting
title Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting
spellingShingle Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting
Alves Fernandes, Alan Carlos
AGRP
CRF
DREADD
FASTING
HPA AXIS
title_short Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting
title_full Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting
title_fullStr Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting
title_full_unstemmed Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting
title_sort Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting
dc.creator.none.fl_str_mv Alves Fernandes, Alan Carlos
Pereira de Oliveira, Franciane
Fernandez, Gimena
da Guia Vieira, Luane
Gugelmin Rosa, Cristiane
do Nascimento, Taís
de Castro França, Suzelei
Donato Jr, Jose
Vella, Kristen R.
Antunes Rodrigues, Jose
Mecawi , André
Perello, Mario
Leico Kagohara Elias, Lucila
Rorato, Rodrigo
author Alves Fernandes, Alan Carlos
author_facet Alves Fernandes, Alan Carlos
Pereira de Oliveira, Franciane
Fernandez, Gimena
da Guia Vieira, Luane
Gugelmin Rosa, Cristiane
do Nascimento, Taís
de Castro França, Suzelei
Donato Jr, Jose
Vella, Kristen R.
Antunes Rodrigues, Jose
Mecawi , André
Perello, Mario
Leico Kagohara Elias, Lucila
Rorato, Rodrigo
author_role author
author2 Pereira de Oliveira, Franciane
Fernandez, Gimena
da Guia Vieira, Luane
Gugelmin Rosa, Cristiane
do Nascimento, Taís
de Castro França, Suzelei
Donato Jr, Jose
Vella, Kristen R.
Antunes Rodrigues, Jose
Mecawi , André
Perello, Mario
Leico Kagohara Elias, Lucila
Rorato, Rodrigo
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AGRP
CRF
DREADD
FASTING
HPA AXIS
topic AGRP
CRF
DREADD
FASTING
HPA AXIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: The activation of the hypothalamic–pituitary–adrenal (HPA) axis is essential for metabolic adaptation in response to fasting. However, the neurocircuitry connecting changes in the peripheral energy stores to the activity of hypothalamic paraventricular corticotrophin-releasing factor (CRFPVN) neurons, the master controller of the HPA axis activity, is not completely understood. Our main goal was to determine if hypothalamic arcuate nucleus (ARC) POMC and AgRP neurons can communicate fasting-induced changes in peripheral energy stores, associated to a fall in plasma leptin levels, to CRFPVN neurons to modulate the HPA axis activity in mice. Results: We observed increased plasma corticosterone levels associate with increased CRFPVN mRNA expression and increased CRFPVN neuronal activity in 36 h fasted mice. These responses were associated with a fall in plasma leptin levels and changes in the mRNA expression of Agrp and Pomc in the ARC. Fasting-induced decrease in plasma leptin partially modulated these responses through a change in the activity of ARC neurons. The chemogenetic activation of POMCARC by DREADDs did not affect fasting-induced activation of the HPA axis. DREADDs inhibition of AgRPARC neurons reduced the content of CRFPVN and increased its accumulation in the median eminence but had no effect on corticosterone secretion induced by fasting. Conclusion: Our data indicate that AgRPARC neurons are part of the neurocircuitry involved in the coupling of PVNCRF activity to changes in peripheral energy stores induced by prolonged fasting.
Fil: Alves Fernandes, Alan Carlos. University Of Ribeirao Preto; Brasil
Fil: Pereira de Oliveira, Franciane. Universidade Federal de Sao Paulo.; Brasil
Fil: Fernandez, Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: da Guia Vieira, Luane. University Of Ribeirao Preto; Brasil
Fil: Gugelmin Rosa, Cristiane. University Of Ribeirao Preto; Brasil
Fil: do Nascimento, Taís. University Of Ribeirao Preto; Brasil
Fil: de Castro França, Suzelei. University Of Ribeirao Preto; Brasil
Fil: Donato Jr, Jose. Universidade de Sao Paulo. Departamento de Fisiología; Brasil
Fil: Vella, Kristen R.. Weill Cornell Medical College; Estados Unidos
Fil: Antunes Rodrigues, Jose. University Of Ribeirao Preto; Brasil
Fil: Mecawi , André. Universidade Federal de Sao Paulo.; Brasil
Fil: Perello, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Leico Kagohara Elias, Lucila. University Of Ribeirao Preto; Brasil
Fil: Rorato, Rodrigo. Universidade Federal de Sao Paulo.; Brasil
description Background: The activation of the hypothalamic–pituitary–adrenal (HPA) axis is essential for metabolic adaptation in response to fasting. However, the neurocircuitry connecting changes in the peripheral energy stores to the activity of hypothalamic paraventricular corticotrophin-releasing factor (CRFPVN) neurons, the master controller of the HPA axis activity, is not completely understood. Our main goal was to determine if hypothalamic arcuate nucleus (ARC) POMC and AgRP neurons can communicate fasting-induced changes in peripheral energy stores, associated to a fall in plasma leptin levels, to CRFPVN neurons to modulate the HPA axis activity in mice. Results: We observed increased plasma corticosterone levels associate with increased CRFPVN mRNA expression and increased CRFPVN neuronal activity in 36 h fasted mice. These responses were associated with a fall in plasma leptin levels and changes in the mRNA expression of Agrp and Pomc in the ARC. Fasting-induced decrease in plasma leptin partially modulated these responses through a change in the activity of ARC neurons. The chemogenetic activation of POMCARC by DREADDs did not affect fasting-induced activation of the HPA axis. DREADDs inhibition of AgRPARC neurons reduced the content of CRFPVN and increased its accumulation in the median eminence but had no effect on corticosterone secretion induced by fasting. Conclusion: Our data indicate that AgRPARC neurons are part of the neurocircuitry involved in the coupling of PVNCRF activity to changes in peripheral energy stores induced by prolonged fasting.
publishDate 2022
dc.date.none.fl_str_mv 2022-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/213177
Alves Fernandes, Alan Carlos; Pereira de Oliveira, Franciane; Fernandez, Gimena; da Guia Vieira, Luane; Gugelmin Rosa, Cristiane; et al.; Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting; BioMed Central; Cell and Bioscience; 12; 1; 7-2022; 1-14
2045-3701
CONICET Digital
CONICET
url http://hdl.handle.net/11336/213177
identifier_str_mv Alves Fernandes, Alan Carlos; Pereira de Oliveira, Franciane; Fernandez, Gimena; da Guia Vieira, Luane; Gugelmin Rosa, Cristiane; et al.; Arcuate AgRP, but not POMC neurons, modulate paraventricular CRF synthesis and release in response to fasting; BioMed Central; Cell and Bioscience; 12; 1; 7-2022; 1-14
2045-3701
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/s13578-022-00853-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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