HSV-1 amplicon vectors launch the production of heterologous rotavirus-like particles and induce rotavirus-specific immune responses in mice

Autores
Laimbacher, Andrea S.; Esteban, Laura Emilia; Castello, Alejandro A.; Abdusetir Cerfoglio, Juan Carlos; Argüelles, Marcelo Horacio; Almallo de Glikmann, Graciela; D'antuono, Alejandra Lorena; Mattion, Nora Marta; Berois, Mabel; Arbiza, Juan; Hilbe, Monika; Schraner, Elisabeth M.; Seyffert, Michael; Dresch, Christiane; Epstein, Alberto L.; Ackermann, Mathias; Fraefel, Cornel
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Virus-like particles (VLPs) are promising vaccine candidates because they represent viral antigens in the authentic conformation of the virion and are therefore readily recognized by the immune system. As VLPs do not contain genetic material they are safer than attenuated virus vaccines. In this study, herpes simplex virus type 1 (HSV-1) amplicon vectors were constructed to coexpress the rotavirus (RV) structural genes VP2, VP6, and VP7 and were used as platforms to launch the production of RV-like particles (RVLPs) in vector-infected mammalian cells. Despite the observed splicing of VP6 RNA, full-length VP6 protein and RVLPs were efficiently produced. Intramuscular injection of mice with the amplicon vectors as a two-dose regimen without adjuvants resulted in RV-specific humoral immune responses and, most importantly, immunized mice were partially protected at the mucosal level from challenge with live wild-type (wt) RV. This work provides proof of principle for the application of HSV-1 amplicon vectors that mediate the efficient production of heterologous VLPs as genetic vaccines.
Fil: Laimbacher, Andrea S.. Universitat Zurich; Suiza
Fil: Esteban, Laura Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Castello, Alejandro A.. Universidad Nacional de Quilmes; Argentina
Fil: Abdusetir Cerfoglio, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Argüelles, Marcelo Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Almallo de Glikmann, Graciela. Universidad Nacional de Quilmes; Argentina
Fil: D'antuono, Alejandra Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina
Fil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina
Fil: Berois, Mabel. Universidad de la República; Uruguay
Fil: Arbiza, Juan. Universidad de la República; Uruguay
Fil: Hilbe, Monika. Universitat Zurich; Suiza
Fil: Schraner, Elisabeth M.. Universitat Zurich; Suiza
Fil: Seyffert, Michael. Universitat Zurich; Suiza
Fil: Dresch, Christiane. Universitat Zurich; Suiza
Fil: Epstein, Alberto L.. Centre National de la Recherche Scientifique; Francia. Université de Lyon; Francia
Fil: Ackermann, Mathias. Universitat Zurich; Suiza
Fil: Fraefel, Cornel. Universitat Zurich; Suiza
Materia
HSV-1 amplicon vector
rotavirus-like particles
genetic vaccine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/98555
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/98555
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling HSV-1 amplicon vectors launch the production of heterologous rotavirus-like particles and induce rotavirus-specific immune responses in miceLaimbacher, Andrea S.Esteban, Laura EmiliaCastello, Alejandro A.Abdusetir Cerfoglio, Juan CarlosArgüelles, Marcelo HoracioAlmallo de Glikmann, GracielaD'antuono, Alejandra LorenaMattion, Nora MartaBerois, MabelArbiza, JuanHilbe, MonikaSchraner, Elisabeth M.Seyffert, MichaelDresch, ChristianeEpstein, Alberto L.Ackermann, MathiasFraefel, CornelHSV-1 amplicon vectorrotavirus-like particlesgenetic vaccinehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Virus-like particles (VLPs) are promising vaccine candidates because they represent viral antigens in the authentic conformation of the virion and are therefore readily recognized by the immune system. As VLPs do not contain genetic material they are safer than attenuated virus vaccines. In this study, herpes simplex virus type 1 (HSV-1) amplicon vectors were constructed to coexpress the rotavirus (RV) structural genes VP2, VP6, and VP7 and were used as platforms to launch the production of RV-like particles (RVLPs) in vector-infected mammalian cells. Despite the observed splicing of VP6 RNA, full-length VP6 protein and RVLPs were efficiently produced. Intramuscular injection of mice with the amplicon vectors as a two-dose regimen without adjuvants resulted in RV-specific humoral immune responses and, most importantly, immunized mice were partially protected at the mucosal level from challenge with live wild-type (wt) RV. This work provides proof of principle for the application of HSV-1 amplicon vectors that mediate the efficient production of heterologous VLPs as genetic vaccines.Fil: Laimbacher, Andrea S.. Universitat Zurich; SuizaFil: Esteban, Laura Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; ArgentinaFil: Castello, Alejandro A.. Universidad Nacional de Quilmes; ArgentinaFil: Abdusetir Cerfoglio, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; ArgentinaFil: Argüelles, Marcelo Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; ArgentinaFil: Almallo de Glikmann, Graciela. Universidad Nacional de Quilmes; ArgentinaFil: D'antuono, Alejandra Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; ArgentinaFil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; ArgentinaFil: Berois, Mabel. Universidad de la República; UruguayFil: Arbiza, Juan. Universidad de la República; UruguayFil: Hilbe, Monika. Universitat Zurich; SuizaFil: Schraner, Elisabeth M.. Universitat Zurich; SuizaFil: Seyffert, Michael. Universitat Zurich; SuizaFil: Dresch, Christiane. Universitat Zurich; SuizaFil: Epstein, Alberto L.. Centre National de la Recherche Scientifique; Francia. Université de Lyon; FranciaFil: Ackermann, Mathias. Universitat Zurich; SuizaFil: Fraefel, Cornel. Universitat Zurich; SuizaNature Publishing Group2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/98555Laimbacher, Andrea S.; Esteban, Laura Emilia; Castello, Alejandro A.; Abdusetir Cerfoglio, Juan Carlos; Argüelles, Marcelo Horacio; et al.; HSV-1 amplicon vectors launch the production of heterologous rotavirus-like particles and induce rotavirus-specific immune responses in mice; Nature Publishing Group; Molecular Therapy (print); 20; 9; 11-2011; 1810-18201525-0016CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1525001616321700info:eu-repo/semantics/altIdentifier/doi/10.1038/mt.2012.108info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:07:34Zoai:ri.conicet.gov.ar:11336/98555instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:07:34.773CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv HSV-1 amplicon vectors launch the production of heterologous rotavirus-like particles and induce rotavirus-specific immune responses in mice
title HSV-1 amplicon vectors launch the production of heterologous rotavirus-like particles and induce rotavirus-specific immune responses in mice
spellingShingle HSV-1 amplicon vectors launch the production of heterologous rotavirus-like particles and induce rotavirus-specific immune responses in mice
Laimbacher, Andrea S.
HSV-1 amplicon vector
rotavirus-like particles
genetic vaccine
title_short HSV-1 amplicon vectors launch the production of heterologous rotavirus-like particles and induce rotavirus-specific immune responses in mice
title_full HSV-1 amplicon vectors launch the production of heterologous rotavirus-like particles and induce rotavirus-specific immune responses in mice
title_fullStr HSV-1 amplicon vectors launch the production of heterologous rotavirus-like particles and induce rotavirus-specific immune responses in mice
title_full_unstemmed HSV-1 amplicon vectors launch the production of heterologous rotavirus-like particles and induce rotavirus-specific immune responses in mice
title_sort HSV-1 amplicon vectors launch the production of heterologous rotavirus-like particles and induce rotavirus-specific immune responses in mice
dc.creator.none.fl_str_mv Laimbacher, Andrea S.
Esteban, Laura Emilia
Castello, Alejandro A.
Abdusetir Cerfoglio, Juan Carlos
Argüelles, Marcelo Horacio
Almallo de Glikmann, Graciela
D'antuono, Alejandra Lorena
Mattion, Nora Marta
Berois, Mabel
Arbiza, Juan
Hilbe, Monika
Schraner, Elisabeth M.
Seyffert, Michael
Dresch, Christiane
Epstein, Alberto L.
Ackermann, Mathias
Fraefel, Cornel
author Laimbacher, Andrea S.
author_facet Laimbacher, Andrea S.
Esteban, Laura Emilia
Castello, Alejandro A.
Abdusetir Cerfoglio, Juan Carlos
Argüelles, Marcelo Horacio
Almallo de Glikmann, Graciela
D'antuono, Alejandra Lorena
Mattion, Nora Marta
Berois, Mabel
Arbiza, Juan
Hilbe, Monika
Schraner, Elisabeth M.
Seyffert, Michael
Dresch, Christiane
Epstein, Alberto L.
Ackermann, Mathias
Fraefel, Cornel
author_role author
author2 Esteban, Laura Emilia
Castello, Alejandro A.
Abdusetir Cerfoglio, Juan Carlos
Argüelles, Marcelo Horacio
Almallo de Glikmann, Graciela
D'antuono, Alejandra Lorena
Mattion, Nora Marta
Berois, Mabel
Arbiza, Juan
Hilbe, Monika
Schraner, Elisabeth M.
Seyffert, Michael
Dresch, Christiane
Epstein, Alberto L.
Ackermann, Mathias
Fraefel, Cornel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HSV-1 amplicon vector
rotavirus-like particles
genetic vaccine
topic HSV-1 amplicon vector
rotavirus-like particles
genetic vaccine
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Virus-like particles (VLPs) are promising vaccine candidates because they represent viral antigens in the authentic conformation of the virion and are therefore readily recognized by the immune system. As VLPs do not contain genetic material they are safer than attenuated virus vaccines. In this study, herpes simplex virus type 1 (HSV-1) amplicon vectors were constructed to coexpress the rotavirus (RV) structural genes VP2, VP6, and VP7 and were used as platforms to launch the production of RV-like particles (RVLPs) in vector-infected mammalian cells. Despite the observed splicing of VP6 RNA, full-length VP6 protein and RVLPs were efficiently produced. Intramuscular injection of mice with the amplicon vectors as a two-dose regimen without adjuvants resulted in RV-specific humoral immune responses and, most importantly, immunized mice were partially protected at the mucosal level from challenge with live wild-type (wt) RV. This work provides proof of principle for the application of HSV-1 amplicon vectors that mediate the efficient production of heterologous VLPs as genetic vaccines.
Fil: Laimbacher, Andrea S.. Universitat Zurich; Suiza
Fil: Esteban, Laura Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Castello, Alejandro A.. Universidad Nacional de Quilmes; Argentina
Fil: Abdusetir Cerfoglio, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Argüelles, Marcelo Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes; Argentina
Fil: Almallo de Glikmann, Graciela. Universidad Nacional de Quilmes; Argentina
Fil: D'antuono, Alejandra Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina
Fil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina
Fil: Berois, Mabel. Universidad de la República; Uruguay
Fil: Arbiza, Juan. Universidad de la República; Uruguay
Fil: Hilbe, Monika. Universitat Zurich; Suiza
Fil: Schraner, Elisabeth M.. Universitat Zurich; Suiza
Fil: Seyffert, Michael. Universitat Zurich; Suiza
Fil: Dresch, Christiane. Universitat Zurich; Suiza
Fil: Epstein, Alberto L.. Centre National de la Recherche Scientifique; Francia. Université de Lyon; Francia
Fil: Ackermann, Mathias. Universitat Zurich; Suiza
Fil: Fraefel, Cornel. Universitat Zurich; Suiza
description Virus-like particles (VLPs) are promising vaccine candidates because they represent viral antigens in the authentic conformation of the virion and are therefore readily recognized by the immune system. As VLPs do not contain genetic material they are safer than attenuated virus vaccines. In this study, herpes simplex virus type 1 (HSV-1) amplicon vectors were constructed to coexpress the rotavirus (RV) structural genes VP2, VP6, and VP7 and were used as platforms to launch the production of RV-like particles (RVLPs) in vector-infected mammalian cells. Despite the observed splicing of VP6 RNA, full-length VP6 protein and RVLPs were efficiently produced. Intramuscular injection of mice with the amplicon vectors as a two-dose regimen without adjuvants resulted in RV-specific humoral immune responses and, most importantly, immunized mice were partially protected at the mucosal level from challenge with live wild-type (wt) RV. This work provides proof of principle for the application of HSV-1 amplicon vectors that mediate the efficient production of heterologous VLPs as genetic vaccines.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/98555
Laimbacher, Andrea S.; Esteban, Laura Emilia; Castello, Alejandro A.; Abdusetir Cerfoglio, Juan Carlos; Argüelles, Marcelo Horacio; et al.; HSV-1 amplicon vectors launch the production of heterologous rotavirus-like particles and induce rotavirus-specific immune responses in mice; Nature Publishing Group; Molecular Therapy (print); 20; 9; 11-2011; 1810-1820
1525-0016
CONICET Digital
CONICET
url http://hdl.handle.net/11336/98555
identifier_str_mv Laimbacher, Andrea S.; Esteban, Laura Emilia; Castello, Alejandro A.; Abdusetir Cerfoglio, Juan Carlos; Argüelles, Marcelo Horacio; et al.; HSV-1 amplicon vectors launch the production of heterologous rotavirus-like particles and induce rotavirus-specific immune responses in mice; Nature Publishing Group; Molecular Therapy (print); 20; 9; 11-2011; 1810-1820
1525-0016
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1525001616321700
info:eu-repo/semantics/altIdentifier/doi/10.1038/mt.2012.108
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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