Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors
- Autores
- Palacios, Carlos Adolfo; Claus, Juan Daniel; Mattion, Nora Marta
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- HSV-1 vectors were developed for the expression of several rotavirus genes. Genes from other pathogens were also expressed, thus generating a set of tools to be tested as new vaccine candidates against In this section, we show two different HSV-1 vectors that express the VP6 protein from different rotavirus strains. Figure 1 shows a defective recombinant virus expressing simultaneously EGFP and VP6 from murine rotavirus strain EC, In Figure 2, an amplicon vector overexpressing VP6 from a Wa strain of human Rotavirus is shown. In both cases, the VP6 proteins were recognized by a monoclonal antibody and were found extensively expressed forming filaments along the cells, showing a typical intra-cellular pattern, in the absence of other rotavirus proteins. These results show that the recombinant proteins maintain the specific capacity of self-assembly of the native protein and, therefore, are potential vaccine candidates to be explored.
Fil: Palacios, Carlos Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Claus, Juan Daniel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina - Materia
-
herpesvirus vectors
rotavirus
VP6
recombinant protein - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/4043
Ver los metadatos del registro completo
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Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectorsExpresión en cultivo celular de la proteína VP6 de rotavirus mediante el uso de vectores virales basados en HSV-1Palacios, Carlos AdolfoClaus, Juan DanielMattion, Nora Martaherpesvirus vectorsrotavirusVP6recombinant proteinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1HSV-1 vectors were developed for the expression of several rotavirus genes. Genes from other pathogens were also expressed, thus generating a set of tools to be tested as new vaccine candidates against In this section, we show two different HSV-1 vectors that express the VP6 protein from different rotavirus strains. Figure 1 shows a defective recombinant virus expressing simultaneously EGFP and VP6 from murine rotavirus strain EC, In Figure 2, an amplicon vector overexpressing VP6 from a Wa strain of human Rotavirus is shown. In both cases, the VP6 proteins were recognized by a monoclonal antibody and were found extensively expressed forming filaments along the cells, showing a typical intra-cellular pattern, in the absence of other rotavirus proteins. These results show that the recombinant proteins maintain the specific capacity of self-assembly of the native protein and, therefore, are potential vaccine candidates to be explored.Fil: Palacios, Carlos Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: Claus, Juan Daniel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaAsociación Argentina de Microbiología2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4043Palacios, Carlos Adolfo; Claus, Juan Daniel; Mattion, Nora Marta; Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 47; 1; 2-2015; 80-810325-75411851-7617enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0325754115000127info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ram.2014.10.002info:eu-repo/semantics/altIdentifier/url/http://www.redalyc.org/articulo.oa?id=213038579016info:eu-repo/semantics/altIdentifier/issn/0325-7541info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:39:30Zoai:ri.conicet.gov.ar:11336/4043instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:39:31.141CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors Expresión en cultivo celular de la proteína VP6 de rotavirus mediante el uso de vectores virales basados en HSV-1 |
title |
Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors |
spellingShingle |
Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors Palacios, Carlos Adolfo herpesvirus vectors rotavirus VP6 recombinant protein |
title_short |
Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors |
title_full |
Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors |
title_fullStr |
Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors |
title_full_unstemmed |
Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors |
title_sort |
Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors |
dc.creator.none.fl_str_mv |
Palacios, Carlos Adolfo Claus, Juan Daniel Mattion, Nora Marta |
author |
Palacios, Carlos Adolfo |
author_facet |
Palacios, Carlos Adolfo Claus, Juan Daniel Mattion, Nora Marta |
author_role |
author |
author2 |
Claus, Juan Daniel Mattion, Nora Marta |
author2_role |
author author |
dc.subject.none.fl_str_mv |
herpesvirus vectors rotavirus VP6 recombinant protein |
topic |
herpesvirus vectors rotavirus VP6 recombinant protein |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
HSV-1 vectors were developed for the expression of several rotavirus genes. Genes from other pathogens were also expressed, thus generating a set of tools to be tested as new vaccine candidates against In this section, we show two different HSV-1 vectors that express the VP6 protein from different rotavirus strains. Figure 1 shows a defective recombinant virus expressing simultaneously EGFP and VP6 from murine rotavirus strain EC, In Figure 2, an amplicon vector overexpressing VP6 from a Wa strain of human Rotavirus is shown. In both cases, the VP6 proteins were recognized by a monoclonal antibody and were found extensively expressed forming filaments along the cells, showing a typical intra-cellular pattern, in the absence of other rotavirus proteins. These results show that the recombinant proteins maintain the specific capacity of self-assembly of the native protein and, therefore, are potential vaccine candidates to be explored. Fil: Palacios, Carlos Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina Fil: Claus, Juan Daniel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina Fil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina |
description |
HSV-1 vectors were developed for the expression of several rotavirus genes. Genes from other pathogens were also expressed, thus generating a set of tools to be tested as new vaccine candidates against In this section, we show two different HSV-1 vectors that express the VP6 protein from different rotavirus strains. Figure 1 shows a defective recombinant virus expressing simultaneously EGFP and VP6 from murine rotavirus strain EC, In Figure 2, an amplicon vector overexpressing VP6 from a Wa strain of human Rotavirus is shown. In both cases, the VP6 proteins were recognized by a monoclonal antibody and were found extensively expressed forming filaments along the cells, showing a typical intra-cellular pattern, in the absence of other rotavirus proteins. These results show that the recombinant proteins maintain the specific capacity of self-assembly of the native protein and, therefore, are potential vaccine candidates to be explored. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/4043 Palacios, Carlos Adolfo; Claus, Juan Daniel; Mattion, Nora Marta; Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 47; 1; 2-2015; 80-81 0325-7541 1851-7617 |
url |
http://hdl.handle.net/11336/4043 |
identifier_str_mv |
Palacios, Carlos Adolfo; Claus, Juan Daniel; Mattion, Nora Marta; Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 47; 1; 2-2015; 80-81 0325-7541 1851-7617 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0325754115000127 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ram.2014.10.002 info:eu-repo/semantics/altIdentifier/url/http://www.redalyc.org/articulo.oa?id=213038579016 info:eu-repo/semantics/altIdentifier/issn/0325-7541 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Asociación Argentina de Microbiología |
publisher.none.fl_str_mv |
Asociación Argentina de Microbiología |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |