Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors

Autores
Palacios, Carlos Adolfo; Claus, Juan Daniel; Mattion, Nora Marta
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
HSV-1 vectors were developed for the expression of several rotavirus genes. Genes from other pathogens were also expressed, thus generating a set of tools to be tested as new vaccine candidates against In this section, we show two different HSV-1 vectors that express the VP6 protein from different rotavirus strains. Figure 1 shows a defective recombinant virus expressing simultaneously EGFP and VP6 from murine rotavirus strain EC, In Figure 2, an amplicon vector overexpressing VP6 from a Wa strain of human Rotavirus is shown. In both cases, the VP6 proteins were recognized by a monoclonal antibody and were found extensively expressed forming filaments along the cells, showing a typical intra-cellular pattern, in the absence of other rotavirus proteins. These results show that the recombinant proteins maintain the specific capacity of self-assembly of the native protein and, therefore, are potential vaccine candidates to be explored.
Fil: Palacios, Carlos Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Claus, Juan Daniel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Materia
herpesvirus vectors
rotavirus
VP6
recombinant protein
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4043
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4043
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectorsExpresión en cultivo celular de la proteína VP6 de rotavirus mediante el uso de vectores virales basados en HSV-1Palacios, Carlos AdolfoClaus, Juan DanielMattion, Nora Martaherpesvirus vectorsrotavirusVP6recombinant proteinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1HSV-1 vectors were developed for the expression of several rotavirus genes. Genes from other pathogens were also expressed, thus generating a set of tools to be tested as new vaccine candidates against In this section, we show two different HSV-1 vectors that express the VP6 protein from different rotavirus strains. Figure 1 shows a defective recombinant virus expressing simultaneously EGFP and VP6 from murine rotavirus strain EC, In Figure 2, an amplicon vector overexpressing VP6 from a Wa strain of human Rotavirus is shown. In both cases, the VP6 proteins were recognized by a monoclonal antibody and were found extensively expressed forming filaments along the cells, showing a typical intra-cellular pattern, in the absence of other rotavirus proteins. These results show that the recombinant proteins maintain the specific capacity of self-assembly of the native protein and, therefore, are potential vaccine candidates to be explored.Fil: Palacios, Carlos Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: Claus, Juan Daniel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaAsociación Argentina de Microbiología2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4043Palacios, Carlos Adolfo; Claus, Juan Daniel; Mattion, Nora Marta; Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 47; 1; 2-2015; 80-810325-75411851-7617enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0325754115000127info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ram.2014.10.002info:eu-repo/semantics/altIdentifier/url/http://www.redalyc.org/articulo.oa?id=213038579016info:eu-repo/semantics/altIdentifier/issn/0325-7541info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:46:04Zoai:ri.conicet.gov.ar:11336/4043instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:46:05.277CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors
Expresión en cultivo celular de la proteína VP6 de rotavirus mediante el uso de vectores virales basados en HSV-1
title Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors
spellingShingle Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors
Palacios, Carlos Adolfo
herpesvirus vectors
rotavirus
VP6
recombinant protein
title_short Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors
title_full Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors
title_fullStr Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors
title_full_unstemmed Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors
title_sort Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors
dc.creator.none.fl_str_mv Palacios, Carlos Adolfo
Claus, Juan Daniel
Mattion, Nora Marta
author Palacios, Carlos Adolfo
author_facet Palacios, Carlos Adolfo
Claus, Juan Daniel
Mattion, Nora Marta
author_role author
author2 Claus, Juan Daniel
Mattion, Nora Marta
author2_role author
author
dc.subject.none.fl_str_mv herpesvirus vectors
rotavirus
VP6
recombinant protein
topic herpesvirus vectors
rotavirus
VP6
recombinant protein
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv HSV-1 vectors were developed for the expression of several rotavirus genes. Genes from other pathogens were also expressed, thus generating a set of tools to be tested as new vaccine candidates against In this section, we show two different HSV-1 vectors that express the VP6 protein from different rotavirus strains. Figure 1 shows a defective recombinant virus expressing simultaneously EGFP and VP6 from murine rotavirus strain EC, In Figure 2, an amplicon vector overexpressing VP6 from a Wa strain of human Rotavirus is shown. In both cases, the VP6 proteins were recognized by a monoclonal antibody and were found extensively expressed forming filaments along the cells, showing a typical intra-cellular pattern, in the absence of other rotavirus proteins. These results show that the recombinant proteins maintain the specific capacity of self-assembly of the native protein and, therefore, are potential vaccine candidates to be explored.
Fil: Palacios, Carlos Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Claus, Juan Daniel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Mattion, Nora Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
description HSV-1 vectors were developed for the expression of several rotavirus genes. Genes from other pathogens were also expressed, thus generating a set of tools to be tested as new vaccine candidates against In this section, we show two different HSV-1 vectors that express the VP6 protein from different rotavirus strains. Figure 1 shows a defective recombinant virus expressing simultaneously EGFP and VP6 from murine rotavirus strain EC, In Figure 2, an amplicon vector overexpressing VP6 from a Wa strain of human Rotavirus is shown. In both cases, the VP6 proteins were recognized by a monoclonal antibody and were found extensively expressed forming filaments along the cells, showing a typical intra-cellular pattern, in the absence of other rotavirus proteins. These results show that the recombinant proteins maintain the specific capacity of self-assembly of the native protein and, therefore, are potential vaccine candidates to be explored.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4043
Palacios, Carlos Adolfo; Claus, Juan Daniel; Mattion, Nora Marta; Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 47; 1; 2-2015; 80-81
0325-7541
1851-7617
url http://hdl.handle.net/11336/4043
identifier_str_mv Palacios, Carlos Adolfo; Claus, Juan Daniel; Mattion, Nora Marta; Rotavirus VP6 protein expressed in cell culture by HSV-1-based vectors; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 47; 1; 2-2015; 80-81
0325-7541
1851-7617
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0325754115000127
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ram.2014.10.002
info:eu-repo/semantics/altIdentifier/url/http://www.redalyc.org/articulo.oa?id=213038579016
info:eu-repo/semantics/altIdentifier/issn/0325-7541
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Asociación Argentina de Microbiología
publisher.none.fl_str_mv Asociación Argentina de Microbiología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.10058

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