Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens

Autores
Molinari, Maria Paula; Peralta, Andrea Verónica; Maletto, Belkys Angélica; Pistoresi, Maria Cristina; Crespo, Maria Ines; Moron, Victor Gabriel; Taboga, Oscar Alberto
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The first 92 amino acids of VP2 of rotavirus are dispensable for VLP assembly and can be replaced by heterologous reporter proteins without affecting either self-assembly of chimeric VP2 or the interaction with VP6 to render chimeric VP2/6 VLPs. In this study, we constructed recombinant baculoviruses expressing GFP or OVA fused to the amino terminus of a truncated VP2 sequence and produced chimeric VLPs by co-infection with a baculovirus expressing VP6. The results showed that these chimeric VLPs were efficiently uptaken by murine dendritic cells and that the heterologous sequences contained in the core of these VLPs seemed to be able to reach the MHC-I pathway as they elicited strong and specific CTL responses. Therefore, the data presented here suggest that chimeric VLPs could be used as excellent carriers to elicit CTL responses to antigens transported inside the VLPs
Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maletto, Belkys Angélica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Pistoresi, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Crespo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Moron, Victor Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Materia
Ctl Responses
Heterologous Antigens
Rotavirus-Derived Dlps as Carrier
Vector Vaccine
Baculovirus System
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31959

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigensMolinari, Maria PaulaPeralta, Andrea VerónicaMaletto, Belkys AngélicaPistoresi, Maria CristinaCrespo, Maria InesMoron, Victor GabrielTaboga, Oscar AlbertoCtl ResponsesHeterologous AntigensRotavirus-Derived Dlps as CarrierVector VaccineBaculovirus Systemhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The first 92 amino acids of VP2 of rotavirus are dispensable for VLP assembly and can be replaced by heterologous reporter proteins without affecting either self-assembly of chimeric VP2 or the interaction with VP6 to render chimeric VP2/6 VLPs. In this study, we constructed recombinant baculoviruses expressing GFP or OVA fused to the amino terminus of a truncated VP2 sequence and produced chimeric VLPs by co-infection with a baculovirus expressing VP6. The results showed that these chimeric VLPs were efficiently uptaken by murine dendritic cells and that the heterologous sequences contained in the core of these VLPs seemed to be able to reach the MHC-I pathway as they elicited strong and specific CTL responses. Therefore, the data presented here suggest that chimeric VLPs could be used as excellent carriers to elicit CTL responses to antigens transported inside the VLPsFil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Maletto, Belkys Angélica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Pistoresi, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Crespo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Moron, Victor Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaElsevier2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31959Taboga, Oscar Alberto; Moron, Victor Gabriel; Crespo, Maria Ines; Pistoresi, Maria Cristina; Maletto, Belkys Angélica; Peralta, Andrea Verónica; et al.; Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens; Elsevier; Process Biochemistry; 49; 11; 7-2014; 1929-19351359-5113CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2014.07.014info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1359511314004000info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:11Zoai:ri.conicet.gov.ar:11336/31959instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:12.148CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens
title Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens
spellingShingle Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens
Molinari, Maria Paula
Ctl Responses
Heterologous Antigens
Rotavirus-Derived Dlps as Carrier
Vector Vaccine
Baculovirus System
title_short Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens
title_full Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens
title_fullStr Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens
title_full_unstemmed Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens
title_sort Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens
dc.creator.none.fl_str_mv Molinari, Maria Paula
Peralta, Andrea Verónica
Maletto, Belkys Angélica
Pistoresi, Maria Cristina
Crespo, Maria Ines
Moron, Victor Gabriel
Taboga, Oscar Alberto
author Molinari, Maria Paula
author_facet Molinari, Maria Paula
Peralta, Andrea Verónica
Maletto, Belkys Angélica
Pistoresi, Maria Cristina
Crespo, Maria Ines
Moron, Victor Gabriel
Taboga, Oscar Alberto
author_role author
author2 Peralta, Andrea Verónica
Maletto, Belkys Angélica
Pistoresi, Maria Cristina
Crespo, Maria Ines
Moron, Victor Gabriel
Taboga, Oscar Alberto
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ctl Responses
Heterologous Antigens
Rotavirus-Derived Dlps as Carrier
Vector Vaccine
Baculovirus System
topic Ctl Responses
Heterologous Antigens
Rotavirus-Derived Dlps as Carrier
Vector Vaccine
Baculovirus System
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The first 92 amino acids of VP2 of rotavirus are dispensable for VLP assembly and can be replaced by heterologous reporter proteins without affecting either self-assembly of chimeric VP2 or the interaction with VP6 to render chimeric VP2/6 VLPs. In this study, we constructed recombinant baculoviruses expressing GFP or OVA fused to the amino terminus of a truncated VP2 sequence and produced chimeric VLPs by co-infection with a baculovirus expressing VP6. The results showed that these chimeric VLPs were efficiently uptaken by murine dendritic cells and that the heterologous sequences contained in the core of these VLPs seemed to be able to reach the MHC-I pathway as they elicited strong and specific CTL responses. Therefore, the data presented here suggest that chimeric VLPs could be used as excellent carriers to elicit CTL responses to antigens transported inside the VLPs
Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maletto, Belkys Angélica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Pistoresi, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Crespo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Moron, Victor Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
description The first 92 amino acids of VP2 of rotavirus are dispensable for VLP assembly and can be replaced by heterologous reporter proteins without affecting either self-assembly of chimeric VP2 or the interaction with VP6 to render chimeric VP2/6 VLPs. In this study, we constructed recombinant baculoviruses expressing GFP or OVA fused to the amino terminus of a truncated VP2 sequence and produced chimeric VLPs by co-infection with a baculovirus expressing VP6. The results showed that these chimeric VLPs were efficiently uptaken by murine dendritic cells and that the heterologous sequences contained in the core of these VLPs seemed to be able to reach the MHC-I pathway as they elicited strong and specific CTL responses. Therefore, the data presented here suggest that chimeric VLPs could be used as excellent carriers to elicit CTL responses to antigens transported inside the VLPs
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31959
Taboga, Oscar Alberto; Moron, Victor Gabriel; Crespo, Maria Ines; Pistoresi, Maria Cristina; Maletto, Belkys Angélica; Peralta, Andrea Verónica; et al.; Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens; Elsevier; Process Biochemistry; 49; 11; 7-2014; 1929-1935
1359-5113
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31959
identifier_str_mv Taboga, Oscar Alberto; Moron, Victor Gabriel; Crespo, Maria Ines; Pistoresi, Maria Cristina; Maletto, Belkys Angélica; Peralta, Andrea Verónica; et al.; Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens; Elsevier; Process Biochemistry; 49; 11; 7-2014; 1929-1935
1359-5113
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2014.07.014
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1359511314004000
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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