Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens
- Autores
- Molinari, Maria Paula; Peralta, Andrea Verónica; Maletto, Belkys Angélica; Pistoresi, Maria Cristina; Crespo, Maria Ines; Moron, Victor Gabriel; Taboga, Oscar Alberto
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The first 92 amino acids of VP2 of rotavirus are dispensable for VLP assembly and can be replaced by heterologous reporter proteins without affecting either self-assembly of chimeric VP2 or the interaction with VP6 to render chimeric VP2/6 VLPs. In this study, we constructed recombinant baculoviruses expressing GFP or OVA fused to the amino terminus of a truncated VP2 sequence and produced chimeric VLPs by co-infection with a baculovirus expressing VP6. The results showed that these chimeric VLPs were efficiently uptaken by murine dendritic cells and that the heterologous sequences contained in the core of these VLPs seemed to be able to reach the MHC-I pathway as they elicited strong and specific CTL responses. Therefore, the data presented here suggest that chimeric VLPs could be used as excellent carriers to elicit CTL responses to antigens transported inside the VLPs
Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maletto, Belkys Angélica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Pistoresi, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Crespo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Moron, Victor Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina - Materia
-
Ctl Responses
Heterologous Antigens
Rotavirus-Derived Dlps as Carrier
Vector Vaccine
Baculovirus System - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/31959
Ver los metadatos del registro completo
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Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigensMolinari, Maria PaulaPeralta, Andrea VerónicaMaletto, Belkys AngélicaPistoresi, Maria CristinaCrespo, Maria InesMoron, Victor GabrielTaboga, Oscar AlbertoCtl ResponsesHeterologous AntigensRotavirus-Derived Dlps as CarrierVector VaccineBaculovirus Systemhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The first 92 amino acids of VP2 of rotavirus are dispensable for VLP assembly and can be replaced by heterologous reporter proteins without affecting either self-assembly of chimeric VP2 or the interaction with VP6 to render chimeric VP2/6 VLPs. In this study, we constructed recombinant baculoviruses expressing GFP or OVA fused to the amino terminus of a truncated VP2 sequence and produced chimeric VLPs by co-infection with a baculovirus expressing VP6. The results showed that these chimeric VLPs were efficiently uptaken by murine dendritic cells and that the heterologous sequences contained in the core of these VLPs seemed to be able to reach the MHC-I pathway as they elicited strong and specific CTL responses. Therefore, the data presented here suggest that chimeric VLPs could be used as excellent carriers to elicit CTL responses to antigens transported inside the VLPsFil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Maletto, Belkys Angélica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Pistoresi, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Crespo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Moron, Victor Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaElsevier2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31959Taboga, Oscar Alberto; Moron, Victor Gabriel; Crespo, Maria Ines; Pistoresi, Maria Cristina; Maletto, Belkys Angélica; Peralta, Andrea Verónica; et al.; Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens; Elsevier; Process Biochemistry; 49; 11; 7-2014; 1929-19351359-5113CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2014.07.014info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1359511314004000info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:11Zoai:ri.conicet.gov.ar:11336/31959instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:12.148CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens |
title |
Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens |
spellingShingle |
Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens Molinari, Maria Paula Ctl Responses Heterologous Antigens Rotavirus-Derived Dlps as Carrier Vector Vaccine Baculovirus System |
title_short |
Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens |
title_full |
Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens |
title_fullStr |
Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens |
title_full_unstemmed |
Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens |
title_sort |
Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens |
dc.creator.none.fl_str_mv |
Molinari, Maria Paula Peralta, Andrea Verónica Maletto, Belkys Angélica Pistoresi, Maria Cristina Crespo, Maria Ines Moron, Victor Gabriel Taboga, Oscar Alberto |
author |
Molinari, Maria Paula |
author_facet |
Molinari, Maria Paula Peralta, Andrea Verónica Maletto, Belkys Angélica Pistoresi, Maria Cristina Crespo, Maria Ines Moron, Victor Gabriel Taboga, Oscar Alberto |
author_role |
author |
author2 |
Peralta, Andrea Verónica Maletto, Belkys Angélica Pistoresi, Maria Cristina Crespo, Maria Ines Moron, Victor Gabriel Taboga, Oscar Alberto |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Ctl Responses Heterologous Antigens Rotavirus-Derived Dlps as Carrier Vector Vaccine Baculovirus System |
topic |
Ctl Responses Heterologous Antigens Rotavirus-Derived Dlps as Carrier Vector Vaccine Baculovirus System |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 https://purl.org/becyt/ford/3.4 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The first 92 amino acids of VP2 of rotavirus are dispensable for VLP assembly and can be replaced by heterologous reporter proteins without affecting either self-assembly of chimeric VP2 or the interaction with VP6 to render chimeric VP2/6 VLPs. In this study, we constructed recombinant baculoviruses expressing GFP or OVA fused to the amino terminus of a truncated VP2 sequence and produced chimeric VLPs by co-infection with a baculovirus expressing VP6. The results showed that these chimeric VLPs were efficiently uptaken by murine dendritic cells and that the heterologous sequences contained in the core of these VLPs seemed to be able to reach the MHC-I pathway as they elicited strong and specific CTL responses. Therefore, the data presented here suggest that chimeric VLPs could be used as excellent carriers to elicit CTL responses to antigens transported inside the VLPs Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Maletto, Belkys Angélica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Pistoresi, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Crespo, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Moron, Victor Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina |
description |
The first 92 amino acids of VP2 of rotavirus are dispensable for VLP assembly and can be replaced by heterologous reporter proteins without affecting either self-assembly of chimeric VP2 or the interaction with VP6 to render chimeric VP2/6 VLPs. In this study, we constructed recombinant baculoviruses expressing GFP or OVA fused to the amino terminus of a truncated VP2 sequence and produced chimeric VLPs by co-infection with a baculovirus expressing VP6. The results showed that these chimeric VLPs were efficiently uptaken by murine dendritic cells and that the heterologous sequences contained in the core of these VLPs seemed to be able to reach the MHC-I pathway as they elicited strong and specific CTL responses. Therefore, the data presented here suggest that chimeric VLPs could be used as excellent carriers to elicit CTL responses to antigens transported inside the VLPs |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/31959 Taboga, Oscar Alberto; Moron, Victor Gabriel; Crespo, Maria Ines; Pistoresi, Maria Cristina; Maletto, Belkys Angélica; Peralta, Andrea Verónica; et al.; Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens; Elsevier; Process Biochemistry; 49; 11; 7-2014; 1929-1935 1359-5113 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/31959 |
identifier_str_mv |
Taboga, Oscar Alberto; Moron, Victor Gabriel; Crespo, Maria Ines; Pistoresi, Maria Cristina; Maletto, Belkys Angélica; Peralta, Andrea Verónica; et al.; Double-layered rotavirus-like particles are efficient carriers to elicit strong CTL responses to delivered heterologous antigens; Elsevier; Process Biochemistry; 49; 11; 7-2014; 1929-1935 1359-5113 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2014.07.014 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1359511314004000 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613665859305472 |
score |
13.070432 |