Genotoxicity of AMPA, the environmental metabolite of glyphosate, assessed by the Comet assay and cytogenetic tests

Autores
Mañas, Fernando Javier; Peralta, Laura; Raviolo, Jose Maria; Garcia Ovando, Hugo; Weyers, A.; Ugnia, Laura Inés; Gonzalez Cid, Marcela Beatriz; Larripa, Irene Beatriz; Gorla, Nora Bibiana Maria
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Formulations containing glyphosate are the most widely used herbicides in the world. AMPA is the major environmental breakdown product of glyphosate. The purpose of this study is to evaluate the in vitro genotoxicity of AMPA using the Comet assay in Hep-2 cells after 4 h of incubation and the chromosome aberration (CA) test in human lymphocytes after 48 h of exposition. Potential in vivo genotoxicity was evaluated through the micronucleus test in mice. In the Comet assay, the level of DNA damage in exposed cells at 2.5-7.5 mM showed a significant increase compared with the control group. In human lymphocytes we found statistically significant clastogenic effect AMPA at 1.8 mM compared with the control group. In vivo, the micronucleus test rendered significant statistical increases at 200-400 mg/kg. AMPA was genotoxic in the three performed tests. Very scarce data are available about AMPA potential genotoxicity. © 2008 Elsevier Inc. All rights reserved.
Fil: Mañas, Fernando Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Peralta, Laura. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Raviolo, Jose Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal; Argentina
Fil: Garcia Ovando, Hugo. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina
Fil: Weyers, A.. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Ugnia, Laura Inés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Gonzalez Cid, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Larripa, Irene Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Gorla, Nora Bibiana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Materia
Ampa
Chromosome Damage
Dna Damage
Glyphosate
Herbicide
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54826
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genotoxicity of AMPA, the environmental metabolite of glyphosate, assessed by the Comet assay and cytogenetic testsMañas, Fernando JavierPeralta, LauraRaviolo, Jose MariaGarcia Ovando, HugoWeyers, A.Ugnia, Laura InésGonzalez Cid, Marcela BeatrizLarripa, Irene BeatrizGorla, Nora Bibiana MariaAmpaChromosome DamageDna DamageGlyphosateHerbicidehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Formulations containing glyphosate are the most widely used herbicides in the world. AMPA is the major environmental breakdown product of glyphosate. The purpose of this study is to evaluate the in vitro genotoxicity of AMPA using the Comet assay in Hep-2 cells after 4 h of incubation and the chromosome aberration (CA) test in human lymphocytes after 48 h of exposition. Potential in vivo genotoxicity was evaluated through the micronucleus test in mice. In the Comet assay, the level of DNA damage in exposed cells at 2.5-7.5 mM showed a significant increase compared with the control group. In human lymphocytes we found statistically significant clastogenic effect AMPA at 1.8 mM compared with the control group. In vivo, the micronucleus test rendered significant statistical increases at 200-400 mg/kg. AMPA was genotoxic in the three performed tests. Very scarce data are available about AMPA potential genotoxicity. © 2008 Elsevier Inc. All rights reserved.Fil: Mañas, Fernando Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Peralta, Laura. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Raviolo, Jose Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal; ArgentinaFil: Garcia Ovando, Hugo. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; ArgentinaFil: Weyers, A.. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Ugnia, Laura Inés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Gonzalez Cid, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Larripa, Irene Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Gorla, Nora Bibiana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaAcademic Press Inc Elsevier Science2009-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54826Mañas, Fernando Javier; Peralta, Laura; Raviolo, Jose Maria; Garcia Ovando, Hugo; Weyers, A.; et al.; Genotoxicity of AMPA, the environmental metabolite of glyphosate, assessed by the Comet assay and cytogenetic tests; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 72; 3; 3-2009; 834-8370147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2008.09.019info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147651308002662info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:51Zoai:ri.conicet.gov.ar:11336/54826instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:51.642CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genotoxicity of AMPA, the environmental metabolite of glyphosate, assessed by the Comet assay and cytogenetic tests
title Genotoxicity of AMPA, the environmental metabolite of glyphosate, assessed by the Comet assay and cytogenetic tests
spellingShingle Genotoxicity of AMPA, the environmental metabolite of glyphosate, assessed by the Comet assay and cytogenetic tests
Mañas, Fernando Javier
Ampa
Chromosome Damage
Dna Damage
Glyphosate
Herbicide
title_short Genotoxicity of AMPA, the environmental metabolite of glyphosate, assessed by the Comet assay and cytogenetic tests
title_full Genotoxicity of AMPA, the environmental metabolite of glyphosate, assessed by the Comet assay and cytogenetic tests
title_fullStr Genotoxicity of AMPA, the environmental metabolite of glyphosate, assessed by the Comet assay and cytogenetic tests
title_full_unstemmed Genotoxicity of AMPA, the environmental metabolite of glyphosate, assessed by the Comet assay and cytogenetic tests
title_sort Genotoxicity of AMPA, the environmental metabolite of glyphosate, assessed by the Comet assay and cytogenetic tests
dc.creator.none.fl_str_mv Mañas, Fernando Javier
Peralta, Laura
Raviolo, Jose Maria
Garcia Ovando, Hugo
Weyers, A.
Ugnia, Laura Inés
Gonzalez Cid, Marcela Beatriz
Larripa, Irene Beatriz
Gorla, Nora Bibiana Maria
author Mañas, Fernando Javier
author_facet Mañas, Fernando Javier
Peralta, Laura
Raviolo, Jose Maria
Garcia Ovando, Hugo
Weyers, A.
Ugnia, Laura Inés
Gonzalez Cid, Marcela Beatriz
Larripa, Irene Beatriz
Gorla, Nora Bibiana Maria
author_role author
author2 Peralta, Laura
Raviolo, Jose Maria
Garcia Ovando, Hugo
Weyers, A.
Ugnia, Laura Inés
Gonzalez Cid, Marcela Beatriz
Larripa, Irene Beatriz
Gorla, Nora Bibiana Maria
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ampa
Chromosome Damage
Dna Damage
Glyphosate
Herbicide
topic Ampa
Chromosome Damage
Dna Damage
Glyphosate
Herbicide
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Formulations containing glyphosate are the most widely used herbicides in the world. AMPA is the major environmental breakdown product of glyphosate. The purpose of this study is to evaluate the in vitro genotoxicity of AMPA using the Comet assay in Hep-2 cells after 4 h of incubation and the chromosome aberration (CA) test in human lymphocytes after 48 h of exposition. Potential in vivo genotoxicity was evaluated through the micronucleus test in mice. In the Comet assay, the level of DNA damage in exposed cells at 2.5-7.5 mM showed a significant increase compared with the control group. In human lymphocytes we found statistically significant clastogenic effect AMPA at 1.8 mM compared with the control group. In vivo, the micronucleus test rendered significant statistical increases at 200-400 mg/kg. AMPA was genotoxic in the three performed tests. Very scarce data are available about AMPA potential genotoxicity. © 2008 Elsevier Inc. All rights reserved.
Fil: Mañas, Fernando Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Peralta, Laura. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Raviolo, Jose Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal; Argentina
Fil: Garcia Ovando, Hugo. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Clínica Animal; Argentina
Fil: Weyers, A.. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Ugnia, Laura Inés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Gonzalez Cid, Marcela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Larripa, Irene Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Gorla, Nora Bibiana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
description Formulations containing glyphosate are the most widely used herbicides in the world. AMPA is the major environmental breakdown product of glyphosate. The purpose of this study is to evaluate the in vitro genotoxicity of AMPA using the Comet assay in Hep-2 cells after 4 h of incubation and the chromosome aberration (CA) test in human lymphocytes after 48 h of exposition. Potential in vivo genotoxicity was evaluated through the micronucleus test in mice. In the Comet assay, the level of DNA damage in exposed cells at 2.5-7.5 mM showed a significant increase compared with the control group. In human lymphocytes we found statistically significant clastogenic effect AMPA at 1.8 mM compared with the control group. In vivo, the micronucleus test rendered significant statistical increases at 200-400 mg/kg. AMPA was genotoxic in the three performed tests. Very scarce data are available about AMPA potential genotoxicity. © 2008 Elsevier Inc. All rights reserved.
publishDate 2009
dc.date.none.fl_str_mv 2009-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54826
Mañas, Fernando Javier; Peralta, Laura; Raviolo, Jose Maria; Garcia Ovando, Hugo; Weyers, A.; et al.; Genotoxicity of AMPA, the environmental metabolite of glyphosate, assessed by the Comet assay and cytogenetic tests; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 72; 3; 3-2009; 834-837
0147-6513
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54826
identifier_str_mv Mañas, Fernando Javier; Peralta, Laura; Raviolo, Jose Maria; Garcia Ovando, Hugo; Weyers, A.; et al.; Genotoxicity of AMPA, the environmental metabolite of glyphosate, assessed by the Comet assay and cytogenetic tests; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 72; 3; 3-2009; 834-837
0147-6513
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2008.09.019
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147651308002662
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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