Chronobiological study of physiological variables and antioxidant metabolism in glicine max

Autores
Pardo DAniela; Gatica CV; Anzulovich AC.; Navigatore Fonzo, Lorena Silvina; Molina As; Golini Rebeca; Perez Chaca, Maria Veronica
Año de publicación
2021
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The Earth rotation exposes most of the planet's species to the environmental daily oscillations. These external oscillations synchronize the internal biological clocks that generate biological rhythms in the organisms, to anticipate the environmental changes. Among these rhythms, the circadian, 24h-rhythms, are the most studied. In plants, the initiation of photosynthesis after sunrise (periodic process) triggers the accumulation of metabolites, including the production of reactive oxygen species (ROS). ROS represent a constant threat to plants, as they react with different cellular components generating oxidative stress. However, plants have developed mechanisms to maintain redox homeostasis. The oscillations in ROS production, removal and signaling would involve the plant biological clock. The functions of clock oscillators in agriculturally important species, such as soybean (Glycine max L.), are increasingly recognized. Soybean is one of the most important and cultivated legumes in the world and constitutes the main export item of Argentina, one of the main producing countries worldwide. Therefore, the objective of this work was to study the influence of the biological clock on the physiology and antioxidant metabolism of Glycine max L. throughout a 24-hour period. For this purpose, soybean cultures were grown in hydroponics for 7 days under standard environmental conditions, with a 14h-light:10h-dark photoperiod. Biochemical (antioxidant enzyme activity, lipid peroxidation and H2O2 levels), physiological (photosynthetic pigment concentration) and molecular (primer design, identification of transcription factor binding sites) parameters, were determined in soybean leaves. Interestingly, we found that Catalase activity (p<0.05), H2O2 levels (p<0.01) and the content of chlorophyll a (p<0.01) and total chlorophyll (p<0.05) display significant daily rhythms in this tissue (Chronos-fit, p<0.05, p<0.05, p<0.05 y p<0.01, and % rhythm: 66.36%, 55.12%, 39.87%, 48.58%, respectively), with their acrophases occurring at ZT 04:18 ± 00:29, ZT 04:53 ± 00:59, ZT 10:41 ± 03:03 y ZT 10:41 ± 01:28, respectively. Even though, carotenoid content shows a significant daily variation (p<0.05), it does not adjust to a cosine curve and therefore, cannot be define as a rhythm. The 24-h oscillations showed here, might suggest the regulation of oxidative metabolism by the biological clock, in the leaves of Glycine max L. an agriculturally important plant. These results would provide useful information for plant manipulation and crop management, taking into account how antioxidant defenses and oxidative stress vary throughout the day.
Fil: Pardo DAniela. Universidad Nacional de San Luis; Argentina
Fil: Gatica CV. Universidad Nacional de San Luis; Argentina
Fil: Anzulovich AC.. Universidad Nacional de San Luis; Argentina
Fil: Navigatore Fonzo, Lorena Silvina. Universidad Nacional de San Luis; Argentina
Fil: Molina As. Universidad Nacional de San Luis; Argentina
Fil: Golini Rebeca. Universidad Nacional de San Luis; Argentina
Fil: Perez Chaca, Maria Veronica. Universidad Nacional de San Luis; Argentina
XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo
Argentina
Sociedad de Biología de Cuyo
Materia
CHRONOBIOLOGICAL STUDY
PHYSIOLOGICAL VARIABLES
ANTIOXIDANT METABOLISM
GLICINE MAX
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181921
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/181921
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chronobiological study of physiological variables and antioxidant metabolism in glicine maxPardo DAnielaGatica CVAnzulovich AC.Navigatore Fonzo, Lorena SilvinaMolina AsGolini RebecaPerez Chaca, Maria VeronicaCHRONOBIOLOGICAL STUDYPHYSIOLOGICAL VARIABLESANTIOXIDANT METABOLISMGLICINE MAXhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The Earth rotation exposes most of the planet's species to the environmental daily oscillations. These external oscillations synchronize the internal biological clocks that generate biological rhythms in the organisms, to anticipate the environmental changes. Among these rhythms, the circadian, 24h-rhythms, are the most studied. In plants, the initiation of photosynthesis after sunrise (periodic process) triggers the accumulation of metabolites, including the production of reactive oxygen species (ROS). ROS represent a constant threat to plants, as they react with different cellular components generating oxidative stress. However, plants have developed mechanisms to maintain redox homeostasis. The oscillations in ROS production, removal and signaling would involve the plant biological clock. The functions of clock oscillators in agriculturally important species, such as soybean (Glycine max L.), are increasingly recognized. Soybean is one of the most important and cultivated legumes in the world and constitutes the main export item of Argentina, one of the main producing countries worldwide. Therefore, the objective of this work was to study the influence of the biological clock on the physiology and antioxidant metabolism of Glycine max L. throughout a 24-hour period. For this purpose, soybean cultures were grown in hydroponics for 7 days under standard environmental conditions, with a 14h-light:10h-dark photoperiod. Biochemical (antioxidant enzyme activity, lipid peroxidation and H2O2 levels), physiological (photosynthetic pigment concentration) and molecular (primer design, identification of transcription factor binding sites) parameters, were determined in soybean leaves. Interestingly, we found that Catalase activity (p<0.05), H2O2 levels (p<0.01) and the content of chlorophyll a (p<0.01) and total chlorophyll (p<0.05) display significant daily rhythms in this tissue (Chronos-fit, p<0.05, p<0.05, p<0.05 y p<0.01, and % rhythm: 66.36%, 55.12%, 39.87%, 48.58%, respectively), with their acrophases occurring at ZT 04:18 ± 00:29, ZT 04:53 ± 00:59, ZT 10:41 ± 03:03 y ZT 10:41 ± 01:28, respectively. Even though, carotenoid content shows a significant daily variation (p<0.05), it does not adjust to a cosine curve and therefore, cannot be define as a rhythm. The 24-h oscillations showed here, might suggest the regulation of oxidative metabolism by the biological clock, in the leaves of Glycine max L. an agriculturally important plant. These results would provide useful information for plant manipulation and crop management, taking into account how antioxidant defenses and oxidative stress vary throughout the day.Fil: Pardo DAniela. Universidad Nacional de San Luis; ArgentinaFil: Gatica CV. Universidad Nacional de San Luis; ArgentinaFil: Anzulovich AC.. Universidad Nacional de San Luis; ArgentinaFil: Navigatore Fonzo, Lorena Silvina. Universidad Nacional de San Luis; ArgentinaFil: Molina As. Universidad Nacional de San Luis; ArgentinaFil: Golini Rebeca. Universidad Nacional de San Luis; ArgentinaFil: Perez Chaca, Maria Veronica. Universidad Nacional de San Luis; ArgentinaXXXIX Reunión Científica Anual de la Sociedad de Biología de CuyoArgentinaSociedad de Biología de CuyoBiocell2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181921Chronobiological study of physiological variables and antioxidant metabolism in glicine max; XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo; Argentina; 2021; 35-35CONICET DigitalCONICETspaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:32:09Zoai:ri.conicet.gov.ar:11336/181921instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:32:09.536CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chronobiological study of physiological variables and antioxidant metabolism in glicine max
title Chronobiological study of physiological variables and antioxidant metabolism in glicine max
spellingShingle Chronobiological study of physiological variables and antioxidant metabolism in glicine max
Pardo DAniela
CHRONOBIOLOGICAL STUDY
PHYSIOLOGICAL VARIABLES
ANTIOXIDANT METABOLISM
GLICINE MAX
title_short Chronobiological study of physiological variables and antioxidant metabolism in glicine max
title_full Chronobiological study of physiological variables and antioxidant metabolism in glicine max
title_fullStr Chronobiological study of physiological variables and antioxidant metabolism in glicine max
title_full_unstemmed Chronobiological study of physiological variables and antioxidant metabolism in glicine max
title_sort Chronobiological study of physiological variables and antioxidant metabolism in glicine max
dc.creator.none.fl_str_mv Pardo DAniela
Gatica CV
Anzulovich AC.
Navigatore Fonzo, Lorena Silvina
Molina As
Golini Rebeca
Perez Chaca, Maria Veronica
author Pardo DAniela
author_facet Pardo DAniela
Gatica CV
Anzulovich AC.
Navigatore Fonzo, Lorena Silvina
Molina As
Golini Rebeca
Perez Chaca, Maria Veronica
author_role author
author2 Gatica CV
Anzulovich AC.
Navigatore Fonzo, Lorena Silvina
Molina As
Golini Rebeca
Perez Chaca, Maria Veronica
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CHRONOBIOLOGICAL STUDY
PHYSIOLOGICAL VARIABLES
ANTIOXIDANT METABOLISM
GLICINE MAX
topic CHRONOBIOLOGICAL STUDY
PHYSIOLOGICAL VARIABLES
ANTIOXIDANT METABOLISM
GLICINE MAX
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The Earth rotation exposes most of the planet's species to the environmental daily oscillations. These external oscillations synchronize the internal biological clocks that generate biological rhythms in the organisms, to anticipate the environmental changes. Among these rhythms, the circadian, 24h-rhythms, are the most studied. In plants, the initiation of photosynthesis after sunrise (periodic process) triggers the accumulation of metabolites, including the production of reactive oxygen species (ROS). ROS represent a constant threat to plants, as they react with different cellular components generating oxidative stress. However, plants have developed mechanisms to maintain redox homeostasis. The oscillations in ROS production, removal and signaling would involve the plant biological clock. The functions of clock oscillators in agriculturally important species, such as soybean (Glycine max L.), are increasingly recognized. Soybean is one of the most important and cultivated legumes in the world and constitutes the main export item of Argentina, one of the main producing countries worldwide. Therefore, the objective of this work was to study the influence of the biological clock on the physiology and antioxidant metabolism of Glycine max L. throughout a 24-hour period. For this purpose, soybean cultures were grown in hydroponics for 7 days under standard environmental conditions, with a 14h-light:10h-dark photoperiod. Biochemical (antioxidant enzyme activity, lipid peroxidation and H2O2 levels), physiological (photosynthetic pigment concentration) and molecular (primer design, identification of transcription factor binding sites) parameters, were determined in soybean leaves. Interestingly, we found that Catalase activity (p<0.05), H2O2 levels (p<0.01) and the content of chlorophyll a (p<0.01) and total chlorophyll (p<0.05) display significant daily rhythms in this tissue (Chronos-fit, p<0.05, p<0.05, p<0.05 y p<0.01, and % rhythm: 66.36%, 55.12%, 39.87%, 48.58%, respectively), with their acrophases occurring at ZT 04:18 ± 00:29, ZT 04:53 ± 00:59, ZT 10:41 ± 03:03 y ZT 10:41 ± 01:28, respectively. Even though, carotenoid content shows a significant daily variation (p<0.05), it does not adjust to a cosine curve and therefore, cannot be define as a rhythm. The 24-h oscillations showed here, might suggest the regulation of oxidative metabolism by the biological clock, in the leaves of Glycine max L. an agriculturally important plant. These results would provide useful information for plant manipulation and crop management, taking into account how antioxidant defenses and oxidative stress vary throughout the day.
Fil: Pardo DAniela. Universidad Nacional de San Luis; Argentina
Fil: Gatica CV. Universidad Nacional de San Luis; Argentina
Fil: Anzulovich AC.. Universidad Nacional de San Luis; Argentina
Fil: Navigatore Fonzo, Lorena Silvina. Universidad Nacional de San Luis; Argentina
Fil: Molina As. Universidad Nacional de San Luis; Argentina
Fil: Golini Rebeca. Universidad Nacional de San Luis; Argentina
Fil: Perez Chaca, Maria Veronica. Universidad Nacional de San Luis; Argentina
XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo
Argentina
Sociedad de Biología de Cuyo
description The Earth rotation exposes most of the planet's species to the environmental daily oscillations. These external oscillations synchronize the internal biological clocks that generate biological rhythms in the organisms, to anticipate the environmental changes. Among these rhythms, the circadian, 24h-rhythms, are the most studied. In plants, the initiation of photosynthesis after sunrise (periodic process) triggers the accumulation of metabolites, including the production of reactive oxygen species (ROS). ROS represent a constant threat to plants, as they react with different cellular components generating oxidative stress. However, plants have developed mechanisms to maintain redox homeostasis. The oscillations in ROS production, removal and signaling would involve the plant biological clock. The functions of clock oscillators in agriculturally important species, such as soybean (Glycine max L.), are increasingly recognized. Soybean is one of the most important and cultivated legumes in the world and constitutes the main export item of Argentina, one of the main producing countries worldwide. Therefore, the objective of this work was to study the influence of the biological clock on the physiology and antioxidant metabolism of Glycine max L. throughout a 24-hour period. For this purpose, soybean cultures were grown in hydroponics for 7 days under standard environmental conditions, with a 14h-light:10h-dark photoperiod. Biochemical (antioxidant enzyme activity, lipid peroxidation and H2O2 levels), physiological (photosynthetic pigment concentration) and molecular (primer design, identification of transcription factor binding sites) parameters, were determined in soybean leaves. Interestingly, we found that Catalase activity (p<0.05), H2O2 levels (p<0.01) and the content of chlorophyll a (p<0.01) and total chlorophyll (p<0.05) display significant daily rhythms in this tissue (Chronos-fit, p<0.05, p<0.05, p<0.05 y p<0.01, and % rhythm: 66.36%, 55.12%, 39.87%, 48.58%, respectively), with their acrophases occurring at ZT 04:18 ± 00:29, ZT 04:53 ± 00:59, ZT 10:41 ± 03:03 y ZT 10:41 ± 01:28, respectively. Even though, carotenoid content shows a significant daily variation (p<0.05), it does not adjust to a cosine curve and therefore, cannot be define as a rhythm. The 24-h oscillations showed here, might suggest the regulation of oxidative metabolism by the biological clock, in the leaves of Glycine max L. an agriculturally important plant. These results would provide useful information for plant manipulation and crop management, taking into account how antioxidant defenses and oxidative stress vary throughout the day.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181921
Chronobiological study of physiological variables and antioxidant metabolism in glicine max; XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo; Argentina; 2021; 35-35
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181921
identifier_str_mv Chronobiological study of physiological variables and antioxidant metabolism in glicine max; XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo; Argentina; 2021; 35-35
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Biocell
publisher.none.fl_str_mv Biocell
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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