Chronobiological study of physiological variables and antioxidant metabolism in glicine max
- Autores
- Pardo DAniela; Gatica CV; Anzulovich AC.; Navigatore Fonzo, Lorena Silvina; Molina As; Golini Rebeca; Perez Chaca, Maria Veronica
- Año de publicación
- 2021
- Idioma
- español castellano
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- The Earth rotation exposes most of the planet's species to the environmental daily oscillations. These external oscillations synchronize the internal biological clocks that generate biological rhythms in the organisms, to anticipate the environmental changes. Among these rhythms, the circadian, 24h-rhythms, are the most studied. In plants, the initiation of photosynthesis after sunrise (periodic process) triggers the accumulation of metabolites, including the production of reactive oxygen species (ROS). ROS represent a constant threat to plants, as they react with different cellular components generating oxidative stress. However, plants have developed mechanisms to maintain redox homeostasis. The oscillations in ROS production, removal and signaling would involve the plant biological clock. The functions of clock oscillators in agriculturally important species, such as soybean (Glycine max L.), are increasingly recognized. Soybean is one of the most important and cultivated legumes in the world and constitutes the main export item of Argentina, one of the main producing countries worldwide. Therefore, the objective of this work was to study the influence of the biological clock on the physiology and antioxidant metabolism of Glycine max L. throughout a 24-hour period. For this purpose, soybean cultures were grown in hydroponics for 7 days under standard environmental conditions, with a 14h-light:10h-dark photoperiod. Biochemical (antioxidant enzyme activity, lipid peroxidation and H2O2 levels), physiological (photosynthetic pigment concentration) and molecular (primer design, identification of transcription factor binding sites) parameters, were determined in soybean leaves. Interestingly, we found that Catalase activity (p<0.05), H2O2 levels (p<0.01) and the content of chlorophyll a (p<0.01) and total chlorophyll (p<0.05) display significant daily rhythms in this tissue (Chronos-fit, p<0.05, p<0.05, p<0.05 y p<0.01, and % rhythm: 66.36%, 55.12%, 39.87%, 48.58%, respectively), with their acrophases occurring at ZT 04:18 ± 00:29, ZT 04:53 ± 00:59, ZT 10:41 ± 03:03 y ZT 10:41 ± 01:28, respectively. Even though, carotenoid content shows a significant daily variation (p<0.05), it does not adjust to a cosine curve and therefore, cannot be define as a rhythm. The 24-h oscillations showed here, might suggest the regulation of oxidative metabolism by the biological clock, in the leaves of Glycine max L. an agriculturally important plant. These results would provide useful information for plant manipulation and crop management, taking into account how antioxidant defenses and oxidative stress vary throughout the day.
Fil: Pardo DAniela. Universidad Nacional de San Luis; Argentina
Fil: Gatica CV. Universidad Nacional de San Luis; Argentina
Fil: Anzulovich AC.. Universidad Nacional de San Luis; Argentina
Fil: Navigatore Fonzo, Lorena Silvina. Universidad Nacional de San Luis; Argentina
Fil: Molina As. Universidad Nacional de San Luis; Argentina
Fil: Golini Rebeca. Universidad Nacional de San Luis; Argentina
Fil: Perez Chaca, Maria Veronica. Universidad Nacional de San Luis; Argentina
XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo
Argentina
Sociedad de Biología de Cuyo - Materia
-
CHRONOBIOLOGICAL STUDY
PHYSIOLOGICAL VARIABLES
ANTIOXIDANT METABOLISM
GLICINE MAX - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/181921
Ver los metadatos del registro completo
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Chronobiological study of physiological variables and antioxidant metabolism in glicine maxPardo DAnielaGatica CVAnzulovich AC.Navigatore Fonzo, Lorena SilvinaMolina AsGolini RebecaPerez Chaca, Maria VeronicaCHRONOBIOLOGICAL STUDYPHYSIOLOGICAL VARIABLESANTIOXIDANT METABOLISMGLICINE MAXhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The Earth rotation exposes most of the planet's species to the environmental daily oscillations. These external oscillations synchronize the internal biological clocks that generate biological rhythms in the organisms, to anticipate the environmental changes. Among these rhythms, the circadian, 24h-rhythms, are the most studied. In plants, the initiation of photosynthesis after sunrise (periodic process) triggers the accumulation of metabolites, including the production of reactive oxygen species (ROS). ROS represent a constant threat to plants, as they react with different cellular components generating oxidative stress. However, plants have developed mechanisms to maintain redox homeostasis. The oscillations in ROS production, removal and signaling would involve the plant biological clock. The functions of clock oscillators in agriculturally important species, such as soybean (Glycine max L.), are increasingly recognized. Soybean is one of the most important and cultivated legumes in the world and constitutes the main export item of Argentina, one of the main producing countries worldwide. Therefore, the objective of this work was to study the influence of the biological clock on the physiology and antioxidant metabolism of Glycine max L. throughout a 24-hour period. For this purpose, soybean cultures were grown in hydroponics for 7 days under standard environmental conditions, with a 14h-light:10h-dark photoperiod. Biochemical (antioxidant enzyme activity, lipid peroxidation and H2O2 levels), physiological (photosynthetic pigment concentration) and molecular (primer design, identification of transcription factor binding sites) parameters, were determined in soybean leaves. Interestingly, we found that Catalase activity (p<0.05), H2O2 levels (p<0.01) and the content of chlorophyll a (p<0.01) and total chlorophyll (p<0.05) display significant daily rhythms in this tissue (Chronos-fit, p<0.05, p<0.05, p<0.05 y p<0.01, and % rhythm: 66.36%, 55.12%, 39.87%, 48.58%, respectively), with their acrophases occurring at ZT 04:18 ± 00:29, ZT 04:53 ± 00:59, ZT 10:41 ± 03:03 y ZT 10:41 ± 01:28, respectively. Even though, carotenoid content shows a significant daily variation (p<0.05), it does not adjust to a cosine curve and therefore, cannot be define as a rhythm. The 24-h oscillations showed here, might suggest the regulation of oxidative metabolism by the biological clock, in the leaves of Glycine max L. an agriculturally important plant. These results would provide useful information for plant manipulation and crop management, taking into account how antioxidant defenses and oxidative stress vary throughout the day.Fil: Pardo DAniela. Universidad Nacional de San Luis; ArgentinaFil: Gatica CV. Universidad Nacional de San Luis; ArgentinaFil: Anzulovich AC.. Universidad Nacional de San Luis; ArgentinaFil: Navigatore Fonzo, Lorena Silvina. Universidad Nacional de San Luis; ArgentinaFil: Molina As. Universidad Nacional de San Luis; ArgentinaFil: Golini Rebeca. Universidad Nacional de San Luis; ArgentinaFil: Perez Chaca, Maria Veronica. Universidad Nacional de San Luis; ArgentinaXXXIX Reunión Científica Anual de la Sociedad de Biología de CuyoArgentinaSociedad de Biología de CuyoBiocell2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181921Chronobiological study of physiological variables and antioxidant metabolism in glicine max; XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo; Argentina; 2021; 35-35CONICET DigitalCONICETspaNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:45Zoai:ri.conicet.gov.ar:11336/181921instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:45.543CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Chronobiological study of physiological variables and antioxidant metabolism in glicine max |
title |
Chronobiological study of physiological variables and antioxidant metabolism in glicine max |
spellingShingle |
Chronobiological study of physiological variables and antioxidant metabolism in glicine max Pardo DAniela CHRONOBIOLOGICAL STUDY PHYSIOLOGICAL VARIABLES ANTIOXIDANT METABOLISM GLICINE MAX |
title_short |
Chronobiological study of physiological variables and antioxidant metabolism in glicine max |
title_full |
Chronobiological study of physiological variables and antioxidant metabolism in glicine max |
title_fullStr |
Chronobiological study of physiological variables and antioxidant metabolism in glicine max |
title_full_unstemmed |
Chronobiological study of physiological variables and antioxidant metabolism in glicine max |
title_sort |
Chronobiological study of physiological variables and antioxidant metabolism in glicine max |
dc.creator.none.fl_str_mv |
Pardo DAniela Gatica CV Anzulovich AC. Navigatore Fonzo, Lorena Silvina Molina As Golini Rebeca Perez Chaca, Maria Veronica |
author |
Pardo DAniela |
author_facet |
Pardo DAniela Gatica CV Anzulovich AC. Navigatore Fonzo, Lorena Silvina Molina As Golini Rebeca Perez Chaca, Maria Veronica |
author_role |
author |
author2 |
Gatica CV Anzulovich AC. Navigatore Fonzo, Lorena Silvina Molina As Golini Rebeca Perez Chaca, Maria Veronica |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
CHRONOBIOLOGICAL STUDY PHYSIOLOGICAL VARIABLES ANTIOXIDANT METABOLISM GLICINE MAX |
topic |
CHRONOBIOLOGICAL STUDY PHYSIOLOGICAL VARIABLES ANTIOXIDANT METABOLISM GLICINE MAX |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
The Earth rotation exposes most of the planet's species to the environmental daily oscillations. These external oscillations synchronize the internal biological clocks that generate biological rhythms in the organisms, to anticipate the environmental changes. Among these rhythms, the circadian, 24h-rhythms, are the most studied. In plants, the initiation of photosynthesis after sunrise (periodic process) triggers the accumulation of metabolites, including the production of reactive oxygen species (ROS). ROS represent a constant threat to plants, as they react with different cellular components generating oxidative stress. However, plants have developed mechanisms to maintain redox homeostasis. The oscillations in ROS production, removal and signaling would involve the plant biological clock. The functions of clock oscillators in agriculturally important species, such as soybean (Glycine max L.), are increasingly recognized. Soybean is one of the most important and cultivated legumes in the world and constitutes the main export item of Argentina, one of the main producing countries worldwide. Therefore, the objective of this work was to study the influence of the biological clock on the physiology and antioxidant metabolism of Glycine max L. throughout a 24-hour period. For this purpose, soybean cultures were grown in hydroponics for 7 days under standard environmental conditions, with a 14h-light:10h-dark photoperiod. Biochemical (antioxidant enzyme activity, lipid peroxidation and H2O2 levels), physiological (photosynthetic pigment concentration) and molecular (primer design, identification of transcription factor binding sites) parameters, were determined in soybean leaves. Interestingly, we found that Catalase activity (p<0.05), H2O2 levels (p<0.01) and the content of chlorophyll a (p<0.01) and total chlorophyll (p<0.05) display significant daily rhythms in this tissue (Chronos-fit, p<0.05, p<0.05, p<0.05 y p<0.01, and % rhythm: 66.36%, 55.12%, 39.87%, 48.58%, respectively), with their acrophases occurring at ZT 04:18 ± 00:29, ZT 04:53 ± 00:59, ZT 10:41 ± 03:03 y ZT 10:41 ± 01:28, respectively. Even though, carotenoid content shows a significant daily variation (p<0.05), it does not adjust to a cosine curve and therefore, cannot be define as a rhythm. The 24-h oscillations showed here, might suggest the regulation of oxidative metabolism by the biological clock, in the leaves of Glycine max L. an agriculturally important plant. These results would provide useful information for plant manipulation and crop management, taking into account how antioxidant defenses and oxidative stress vary throughout the day. Fil: Pardo DAniela. Universidad Nacional de San Luis; Argentina Fil: Gatica CV. Universidad Nacional de San Luis; Argentina Fil: Anzulovich AC.. Universidad Nacional de San Luis; Argentina Fil: Navigatore Fonzo, Lorena Silvina. Universidad Nacional de San Luis; Argentina Fil: Molina As. Universidad Nacional de San Luis; Argentina Fil: Golini Rebeca. Universidad Nacional de San Luis; Argentina Fil: Perez Chaca, Maria Veronica. Universidad Nacional de San Luis; Argentina XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo Argentina Sociedad de Biología de Cuyo |
description |
The Earth rotation exposes most of the planet's species to the environmental daily oscillations. These external oscillations synchronize the internal biological clocks that generate biological rhythms in the organisms, to anticipate the environmental changes. Among these rhythms, the circadian, 24h-rhythms, are the most studied. In plants, the initiation of photosynthesis after sunrise (periodic process) triggers the accumulation of metabolites, including the production of reactive oxygen species (ROS). ROS represent a constant threat to plants, as they react with different cellular components generating oxidative stress. However, plants have developed mechanisms to maintain redox homeostasis. The oscillations in ROS production, removal and signaling would involve the plant biological clock. The functions of clock oscillators in agriculturally important species, such as soybean (Glycine max L.), are increasingly recognized. Soybean is one of the most important and cultivated legumes in the world and constitutes the main export item of Argentina, one of the main producing countries worldwide. Therefore, the objective of this work was to study the influence of the biological clock on the physiology and antioxidant metabolism of Glycine max L. throughout a 24-hour period. For this purpose, soybean cultures were grown in hydroponics for 7 days under standard environmental conditions, with a 14h-light:10h-dark photoperiod. Biochemical (antioxidant enzyme activity, lipid peroxidation and H2O2 levels), physiological (photosynthetic pigment concentration) and molecular (primer design, identification of transcription factor binding sites) parameters, were determined in soybean leaves. Interestingly, we found that Catalase activity (p<0.05), H2O2 levels (p<0.01) and the content of chlorophyll a (p<0.01) and total chlorophyll (p<0.05) display significant daily rhythms in this tissue (Chronos-fit, p<0.05, p<0.05, p<0.05 y p<0.01, and % rhythm: 66.36%, 55.12%, 39.87%, 48.58%, respectively), with their acrophases occurring at ZT 04:18 ± 00:29, ZT 04:53 ± 00:59, ZT 10:41 ± 03:03 y ZT 10:41 ± 01:28, respectively. Even though, carotenoid content shows a significant daily variation (p<0.05), it does not adjust to a cosine curve and therefore, cannot be define as a rhythm. The 24-h oscillations showed here, might suggest the regulation of oxidative metabolism by the biological clock, in the leaves of Glycine max L. an agriculturally important plant. These results would provide useful information for plant manipulation and crop management, taking into account how antioxidant defenses and oxidative stress vary throughout the day. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 |
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http://hdl.handle.net/11336/181921 Chronobiological study of physiological variables and antioxidant metabolism in glicine max; XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo; Argentina; 2021; 35-35 CONICET Digital CONICET |
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http://hdl.handle.net/11336/181921 |
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Chronobiological study of physiological variables and antioxidant metabolism in glicine max; XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo; Argentina; 2021; 35-35 CONICET Digital CONICET |
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