Storage-associated genes and reserves accumulation in soybean cultivars differing in physiological strategies for attaining high seed protein concentration

Autores
Poeta, Florencia Belen; Ochogavía, Ana Claudia; Permingeat, Hugo Raúl; Rotundo, José Luis
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High seed protein concentration (HP) in soybeans [Glycine max (L.) Merr.] is attained by increases in protein content in large seed genotypes (HP large seed) or by reductions in oil and carbohydrates contents in small seed genotypes (HP small seed). We hypothesized that these alternative strategies impact seed development, component accumulation, and gene expression differently. We compared a standard protein commercial genotype with two HP genotypes having contrasting seed size. The HP large seed genotype exhibited the fastest rate and longest period of seed growth and reserves accumulation compared with the HP small genotype. Seed development of these contrasting genotypes was normalized using a moisture depletion framework. Expression levels of some of the genes involved in protein and oil synthesis were lower in the HP small seed genotype compared with the other genotypes. No difference in gene expression was observed between the commercial and the HP large seed genotypes, suggesting a role for assimilate supply controlling high protein concentration based on this strategy. Our results indicate that seed development and gene expression are not necessarily associated with high seed protein concentration per se; a better understanding of seed composition requires acknowledging the contrasting strategies, in terms of seed size, to attain high seed protein concentration.
Fil: Poeta, Florencia Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Ochogavía, Ana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Permingeat, Hugo Raúl. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina
Fil: Rotundo, José Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Materia
Glicine Max
Gene Expression
High Seed Protein Concentration
Seed Storage Proteins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/52850
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Storage-associated genes and reserves accumulation in soybean cultivars differing in physiological strategies for attaining high seed protein concentrationPoeta, Florencia BelenOchogavía, Ana ClaudiaPermingeat, Hugo RaúlRotundo, José LuisGlicine MaxGene ExpressionHigh Seed Protein ConcentrationSeed Storage Proteinshttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4High seed protein concentration (HP) in soybeans [Glycine max (L.) Merr.] is attained by increases in protein content in large seed genotypes (HP large seed) or by reductions in oil and carbohydrates contents in small seed genotypes (HP small seed). We hypothesized that these alternative strategies impact seed development, component accumulation, and gene expression differently. We compared a standard protein commercial genotype with two HP genotypes having contrasting seed size. The HP large seed genotype exhibited the fastest rate and longest period of seed growth and reserves accumulation compared with the HP small genotype. Seed development of these contrasting genotypes was normalized using a moisture depletion framework. Expression levels of some of the genes involved in protein and oil synthesis were lower in the HP small seed genotype compared with the other genotypes. No difference in gene expression was observed between the commercial and the HP large seed genotypes, suggesting a role for assimilate supply controlling high protein concentration based on this strategy. Our results indicate that seed development and gene expression are not necessarily associated with high seed protein concentration per se; a better understanding of seed composition requires acknowledging the contrasting strategies, in terms of seed size, to attain high seed protein concentration.Fil: Poeta, Florencia Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Ochogavía, Ana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Permingeat, Hugo Raúl. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; ArgentinaFil: Rotundo, José Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaCrop Science Society of America2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/52850Poeta, Florencia Belen; Ochogavía, Ana Claudia; Permingeat, Hugo Raúl; Rotundo, José Luis; Storage-associated genes and reserves accumulation in soybean cultivars differing in physiological strategies for attaining high seed protein concentration; Crop Science Society of America; Crop Science; 57; 1; 1-2017; 427-4360011-183XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.2135/cropsci2016.07.0611info:eu-repo/semantics/altIdentifier/url/https://dl.sciencesocieties.org/publications/cs/abstracts/57/1/427info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:46Zoai:ri.conicet.gov.ar:11336/52850instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:47.01CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Storage-associated genes and reserves accumulation in soybean cultivars differing in physiological strategies for attaining high seed protein concentration
title Storage-associated genes and reserves accumulation in soybean cultivars differing in physiological strategies for attaining high seed protein concentration
spellingShingle Storage-associated genes and reserves accumulation in soybean cultivars differing in physiological strategies for attaining high seed protein concentration
Poeta, Florencia Belen
Glicine Max
Gene Expression
High Seed Protein Concentration
Seed Storage Proteins
title_short Storage-associated genes and reserves accumulation in soybean cultivars differing in physiological strategies for attaining high seed protein concentration
title_full Storage-associated genes and reserves accumulation in soybean cultivars differing in physiological strategies for attaining high seed protein concentration
title_fullStr Storage-associated genes and reserves accumulation in soybean cultivars differing in physiological strategies for attaining high seed protein concentration
title_full_unstemmed Storage-associated genes and reserves accumulation in soybean cultivars differing in physiological strategies for attaining high seed protein concentration
title_sort Storage-associated genes and reserves accumulation in soybean cultivars differing in physiological strategies for attaining high seed protein concentration
dc.creator.none.fl_str_mv Poeta, Florencia Belen
Ochogavía, Ana Claudia
Permingeat, Hugo Raúl
Rotundo, José Luis
author Poeta, Florencia Belen
author_facet Poeta, Florencia Belen
Ochogavía, Ana Claudia
Permingeat, Hugo Raúl
Rotundo, José Luis
author_role author
author2 Ochogavía, Ana Claudia
Permingeat, Hugo Raúl
Rotundo, José Luis
author2_role author
author
author
dc.subject.none.fl_str_mv Glicine Max
Gene Expression
High Seed Protein Concentration
Seed Storage Proteins
topic Glicine Max
Gene Expression
High Seed Protein Concentration
Seed Storage Proteins
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv High seed protein concentration (HP) in soybeans [Glycine max (L.) Merr.] is attained by increases in protein content in large seed genotypes (HP large seed) or by reductions in oil and carbohydrates contents in small seed genotypes (HP small seed). We hypothesized that these alternative strategies impact seed development, component accumulation, and gene expression differently. We compared a standard protein commercial genotype with two HP genotypes having contrasting seed size. The HP large seed genotype exhibited the fastest rate and longest period of seed growth and reserves accumulation compared with the HP small genotype. Seed development of these contrasting genotypes was normalized using a moisture depletion framework. Expression levels of some of the genes involved in protein and oil synthesis were lower in the HP small seed genotype compared with the other genotypes. No difference in gene expression was observed between the commercial and the HP large seed genotypes, suggesting a role for assimilate supply controlling high protein concentration based on this strategy. Our results indicate that seed development and gene expression are not necessarily associated with high seed protein concentration per se; a better understanding of seed composition requires acknowledging the contrasting strategies, in terms of seed size, to attain high seed protein concentration.
Fil: Poeta, Florencia Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Ochogavía, Ana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Permingeat, Hugo Raúl. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina
Fil: Rotundo, José Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
description High seed protein concentration (HP) in soybeans [Glycine max (L.) Merr.] is attained by increases in protein content in large seed genotypes (HP large seed) or by reductions in oil and carbohydrates contents in small seed genotypes (HP small seed). We hypothesized that these alternative strategies impact seed development, component accumulation, and gene expression differently. We compared a standard protein commercial genotype with two HP genotypes having contrasting seed size. The HP large seed genotype exhibited the fastest rate and longest period of seed growth and reserves accumulation compared with the HP small genotype. Seed development of these contrasting genotypes was normalized using a moisture depletion framework. Expression levels of some of the genes involved in protein and oil synthesis were lower in the HP small seed genotype compared with the other genotypes. No difference in gene expression was observed between the commercial and the HP large seed genotypes, suggesting a role for assimilate supply controlling high protein concentration based on this strategy. Our results indicate that seed development and gene expression are not necessarily associated with high seed protein concentration per se; a better understanding of seed composition requires acknowledging the contrasting strategies, in terms of seed size, to attain high seed protein concentration.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/52850
Poeta, Florencia Belen; Ochogavía, Ana Claudia; Permingeat, Hugo Raúl; Rotundo, José Luis; Storage-associated genes and reserves accumulation in soybean cultivars differing in physiological strategies for attaining high seed protein concentration; Crop Science Society of America; Crop Science; 57; 1; 1-2017; 427-436
0011-183X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/52850
identifier_str_mv Poeta, Florencia Belen; Ochogavía, Ana Claudia; Permingeat, Hugo Raúl; Rotundo, José Luis; Storage-associated genes and reserves accumulation in soybean cultivars differing in physiological strategies for attaining high seed protein concentration; Crop Science Society of America; Crop Science; 57; 1; 1-2017; 427-436
0011-183X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.2135/cropsci2016.07.0611
info:eu-repo/semantics/altIdentifier/url/https://dl.sciencesocieties.org/publications/cs/abstracts/57/1/427
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Crop Science Society of America
publisher.none.fl_str_mv Crop Science Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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