Single-molecule analysis of steroid receptor and cofactor action in living cells

Autores
Paakinaho, Ville; Presman, Diego Martin; Ball, David A.; Johnson, Thomas A.; Schiltz, R. Louis; Levitt, Peter; Mazza, Davide; Morisaki, Tatsuya; Karpova, Tatiana S.; Hager, Gordon L.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Population-based assays have been employed extensively to investigate the interactions of transcription factors (TFs) with chromatin and are often interpreted in terms of static and sequential binding. However, fluorescence microscopy techniques reveal a more dynamic binding behaviour of TFs in live cells. Here we analyse the strengths and limitations of in vivo single-molecule tracking and performed a comprehensive analysis on the intranuclear dwell times of four steroid receptors and a number of known cofactors. While the absolute residence times estimates can depend on imaging acquisition parameters due to sampling bias, our results indicate that only a small proportion of factors are specifically bound to chromatin at any given time. Interestingly, the glucocorticoid receptor and its cofactors affect each other's dwell times in an asymmetric manner. Overall, our data indicate transient rather than stable TF-cofactors chromatin interactions at response elements at the single-molecule level.
Fil: Paakinaho, Ville. National Institutes of Health; Estados Unidos
Fil: Presman, Diego Martin. National Institutes of Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Ball, David A.. National Institutes of Health; Estados Unidos
Fil: Johnson, Thomas A.. National Institutes of Health; Estados Unidos
Fil: Schiltz, R. Louis. National Institutes of Health; Estados Unidos
Fil: Levitt, Peter. National Institutes of Health; Estados Unidos
Fil: Mazza, Davide. Universita Vita-salute San Raffaele; Italia
Fil: Morisaki, Tatsuya. National Institutes of Health; Estados Unidos
Fil: Karpova, Tatiana S.. National Institutes of Health; Estados Unidos
Fil: Hager, Gordon L.. National Institutes of Health; Estados Unidos
Materia
SINGLE MOLECULE TRACKING
GLUCOCORTICOID RECEPTOR
CHROMATIN
TRANSCRIPTION FACTOR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/65691
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/65691
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Single-molecule analysis of steroid receptor and cofactor action in living cellsPaakinaho, VillePresman, Diego MartinBall, David A.Johnson, Thomas A.Schiltz, R. LouisLevitt, PeterMazza, DavideMorisaki, TatsuyaKarpova, Tatiana S.Hager, Gordon L.SINGLE MOLECULE TRACKINGGLUCOCORTICOID RECEPTORCHROMATINTRANSCRIPTION FACTORhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Population-based assays have been employed extensively to investigate the interactions of transcription factors (TFs) with chromatin and are often interpreted in terms of static and sequential binding. However, fluorescence microscopy techniques reveal a more dynamic binding behaviour of TFs in live cells. Here we analyse the strengths and limitations of in vivo single-molecule tracking and performed a comprehensive analysis on the intranuclear dwell times of four steroid receptors and a number of known cofactors. While the absolute residence times estimates can depend on imaging acquisition parameters due to sampling bias, our results indicate that only a small proportion of factors are specifically bound to chromatin at any given time. Interestingly, the glucocorticoid receptor and its cofactors affect each other's dwell times in an asymmetric manner. Overall, our data indicate transient rather than stable TF-cofactors chromatin interactions at response elements at the single-molecule level.Fil: Paakinaho, Ville. National Institutes of Health; Estados UnidosFil: Presman, Diego Martin. National Institutes of Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Ball, David A.. National Institutes of Health; Estados UnidosFil: Johnson, Thomas A.. National Institutes of Health; Estados UnidosFil: Schiltz, R. Louis. National Institutes of Health; Estados UnidosFil: Levitt, Peter. National Institutes of Health; Estados UnidosFil: Mazza, Davide. Universita Vita-salute San Raffaele; ItaliaFil: Morisaki, Tatsuya. National Institutes of Health; Estados UnidosFil: Karpova, Tatiana S.. National Institutes of Health; Estados UnidosFil: Hager, Gordon L.. National Institutes of Health; Estados UnidosNature Publishing Group2017-06-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/65691Paakinaho, Ville; Presman, Diego Martin; Ball, David A.; Johnson, Thomas A.; Schiltz, R. Louis; et al.; Single-molecule analysis of steroid receptor and cofactor action in living cells; Nature Publishing Group; Nature Communications; 8; 15896; 21-6-2017; 1-142041-1723CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/ncomms15896info:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms15896info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:00Zoai:ri.conicet.gov.ar:11336/65691instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:00.344CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Single-molecule analysis of steroid receptor and cofactor action in living cells
title Single-molecule analysis of steroid receptor and cofactor action in living cells
spellingShingle Single-molecule analysis of steroid receptor and cofactor action in living cells
Paakinaho, Ville
SINGLE MOLECULE TRACKING
GLUCOCORTICOID RECEPTOR
CHROMATIN
TRANSCRIPTION FACTOR
title_short Single-molecule analysis of steroid receptor and cofactor action in living cells
title_full Single-molecule analysis of steroid receptor and cofactor action in living cells
title_fullStr Single-molecule analysis of steroid receptor and cofactor action in living cells
title_full_unstemmed Single-molecule analysis of steroid receptor and cofactor action in living cells
title_sort Single-molecule analysis of steroid receptor and cofactor action in living cells
dc.creator.none.fl_str_mv Paakinaho, Ville
Presman, Diego Martin
Ball, David A.
Johnson, Thomas A.
Schiltz, R. Louis
Levitt, Peter
Mazza, Davide
Morisaki, Tatsuya
Karpova, Tatiana S.
Hager, Gordon L.
author Paakinaho, Ville
author_facet Paakinaho, Ville
Presman, Diego Martin
Ball, David A.
Johnson, Thomas A.
Schiltz, R. Louis
Levitt, Peter
Mazza, Davide
Morisaki, Tatsuya
Karpova, Tatiana S.
Hager, Gordon L.
author_role author
author2 Presman, Diego Martin
Ball, David A.
Johnson, Thomas A.
Schiltz, R. Louis
Levitt, Peter
Mazza, Davide
Morisaki, Tatsuya
Karpova, Tatiana S.
Hager, Gordon L.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv SINGLE MOLECULE TRACKING
GLUCOCORTICOID RECEPTOR
CHROMATIN
TRANSCRIPTION FACTOR
topic SINGLE MOLECULE TRACKING
GLUCOCORTICOID RECEPTOR
CHROMATIN
TRANSCRIPTION FACTOR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Population-based assays have been employed extensively to investigate the interactions of transcription factors (TFs) with chromatin and are often interpreted in terms of static and sequential binding. However, fluorescence microscopy techniques reveal a more dynamic binding behaviour of TFs in live cells. Here we analyse the strengths and limitations of in vivo single-molecule tracking and performed a comprehensive analysis on the intranuclear dwell times of four steroid receptors and a number of known cofactors. While the absolute residence times estimates can depend on imaging acquisition parameters due to sampling bias, our results indicate that only a small proportion of factors are specifically bound to chromatin at any given time. Interestingly, the glucocorticoid receptor and its cofactors affect each other's dwell times in an asymmetric manner. Overall, our data indicate transient rather than stable TF-cofactors chromatin interactions at response elements at the single-molecule level.
Fil: Paakinaho, Ville. National Institutes of Health; Estados Unidos
Fil: Presman, Diego Martin. National Institutes of Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Ball, David A.. National Institutes of Health; Estados Unidos
Fil: Johnson, Thomas A.. National Institutes of Health; Estados Unidos
Fil: Schiltz, R. Louis. National Institutes of Health; Estados Unidos
Fil: Levitt, Peter. National Institutes of Health; Estados Unidos
Fil: Mazza, Davide. Universita Vita-salute San Raffaele; Italia
Fil: Morisaki, Tatsuya. National Institutes of Health; Estados Unidos
Fil: Karpova, Tatiana S.. National Institutes of Health; Estados Unidos
Fil: Hager, Gordon L.. National Institutes of Health; Estados Unidos
description Population-based assays have been employed extensively to investigate the interactions of transcription factors (TFs) with chromatin and are often interpreted in terms of static and sequential binding. However, fluorescence microscopy techniques reveal a more dynamic binding behaviour of TFs in live cells. Here we analyse the strengths and limitations of in vivo single-molecule tracking and performed a comprehensive analysis on the intranuclear dwell times of four steroid receptors and a number of known cofactors. While the absolute residence times estimates can depend on imaging acquisition parameters due to sampling bias, our results indicate that only a small proportion of factors are specifically bound to chromatin at any given time. Interestingly, the glucocorticoid receptor and its cofactors affect each other's dwell times in an asymmetric manner. Overall, our data indicate transient rather than stable TF-cofactors chromatin interactions at response elements at the single-molecule level.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-21
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/65691
Paakinaho, Ville; Presman, Diego Martin; Ball, David A.; Johnson, Thomas A.; Schiltz, R. Louis; et al.; Single-molecule analysis of steroid receptor and cofactor action in living cells; Nature Publishing Group; Nature Communications; 8; 15896; 21-6-2017; 1-14
2041-1723
CONICET Digital
CONICET
url http://hdl.handle.net/11336/65691
identifier_str_mv Paakinaho, Ville; Presman, Diego Martin; Ball, David A.; Johnson, Thomas A.; Schiltz, R. Louis; et al.; Single-molecule analysis of steroid receptor and cofactor action in living cells; Nature Publishing Group; Nature Communications; 8; 15896; 21-6-2017; 1-14
2041-1723
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/ncomms15896
info:eu-repo/semantics/altIdentifier/doi/10.1038/ncomms15896
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269194414981120
score 13.13397

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