Steroid Receptors Reprogram FoxA1 Occupancy through Dynamic Chromatin Transitions

Autores
Swinstead, Erin E.; Miranda, Tina B.; Paakinaho, Ville; Baek, Songjoon; Goldstein, Ido; Hawkins, Mary; Karpova, Tatiana S.; Ball, David; Mazza, Davide; Lavis, Luke D.; Grimm, Jonathan B.; Morisaki, Tatsuya; Grøntved, Lars; Presman, Diego Martin; Hager, Gordon L.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The estrogen receptor (ER), glucocorticoid receptor (GR), and forkhead box protein 1 (FoxA1) are significant factors in breast cancer progression. FoxA1 has been implicated in establishing ER-binding patterns though its unique ability to serve as a pioneer factor. However, the molecular interplay between ER, GR, and FoxA1 requires further investigation. Here we show that ER and GR both have the ability to alter the genomic distribution of the FoxA1 pioneer factor. Single-molecule tracking experiments in live cells reveal a highly dynamic interaction of FoxA1 with chromatin in vivo. Furthermore, the FoxA1 factor is not associated with detectable footprints at its binding sites throughout the genome. These findings support a model wherein interactions between transcription factors and pioneer factors are highly dynamic. Moreover, at a subset of genomic sites, the role of pioneer can be reversed, with the steroid receptors serving to enhance binding of FoxA1.
Fil: Swinstead, Erin E.. National Institutes of Health; Estados Unidos
Fil: Miranda, Tina B.. National Institutes of Health; Estados Unidos
Fil: Paakinaho, Ville. National Institutes of Health; Estados Unidos
Fil: Baek, Songjoon. National Institutes of Health; Estados Unidos
Fil: Goldstein, Ido. National Institutes of Health; Estados Unidos
Fil: Hawkins, Mary. National Institutes of Health; Estados Unidos
Fil: Karpova, Tatiana S.. National Institutes of Health; Estados Unidos
Fil: Ball, David. National Institutes of Health; Estados Unidos
Fil: Mazza, Davide. Universita Vita-salute San Raffaele; Italia
Fil: Lavis, Luke D.. Howard Hughes Medical Institute; Estados Unidos
Fil: Grimm, Jonathan B.. Howard Hughes Medical Institute; Estados Unidos
Fil: Morisaki, Tatsuya. National Institutes of Health; Estados Unidos. State University of Colorado - Fort Collins; Estados Unidos
Fil: Grøntved, Lars. National Institutes of Health; Estados Unidos
Fil: Presman, Diego Martin. National Institutes of Health; Estados Unidos
Fil: Hager, Gordon L.. National Institutes of Health; Estados Unidos
Materia
Foxa1
Chromatin
Single Molecule Tracking
Glucocorticoid Receptor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/60625
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/60625
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Steroid Receptors Reprogram FoxA1 Occupancy through Dynamic Chromatin TransitionsSwinstead, Erin E.Miranda, Tina B.Paakinaho, VilleBaek, SongjoonGoldstein, IdoHawkins, MaryKarpova, Tatiana S.Ball, DavidMazza, DavideLavis, Luke D.Grimm, Jonathan B.Morisaki, TatsuyaGrøntved, LarsPresman, Diego MartinHager, Gordon L.Foxa1ChromatinSingle Molecule TrackingGlucocorticoid Receptorhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The estrogen receptor (ER), glucocorticoid receptor (GR), and forkhead box protein 1 (FoxA1) are significant factors in breast cancer progression. FoxA1 has been implicated in establishing ER-binding patterns though its unique ability to serve as a pioneer factor. However, the molecular interplay between ER, GR, and FoxA1 requires further investigation. Here we show that ER and GR both have the ability to alter the genomic distribution of the FoxA1 pioneer factor. Single-molecule tracking experiments in live cells reveal a highly dynamic interaction of FoxA1 with chromatin in vivo. Furthermore, the FoxA1 factor is not associated with detectable footprints at its binding sites throughout the genome. These findings support a model wherein interactions between transcription factors and pioneer factors are highly dynamic. Moreover, at a subset of genomic sites, the role of pioneer can be reversed, with the steroid receptors serving to enhance binding of FoxA1.Fil: Swinstead, Erin E.. National Institutes of Health; Estados UnidosFil: Miranda, Tina B.. National Institutes of Health; Estados UnidosFil: Paakinaho, Ville. National Institutes of Health; Estados UnidosFil: Baek, Songjoon. National Institutes of Health; Estados UnidosFil: Goldstein, Ido. National Institutes of Health; Estados UnidosFil: Hawkins, Mary. National Institutes of Health; Estados UnidosFil: Karpova, Tatiana S.. National Institutes of Health; Estados UnidosFil: Ball, David. National Institutes of Health; Estados UnidosFil: Mazza, Davide. Universita Vita-salute San Raffaele; ItaliaFil: Lavis, Luke D.. Howard Hughes Medical Institute; Estados UnidosFil: Grimm, Jonathan B.. Howard Hughes Medical Institute; Estados UnidosFil: Morisaki, Tatsuya. National Institutes of Health; Estados Unidos. State University of Colorado - Fort Collins; Estados UnidosFil: Grøntved, Lars. National Institutes of Health; Estados UnidosFil: Presman, Diego Martin. National Institutes of Health; Estados UnidosFil: Hager, Gordon L.. National Institutes of Health; Estados UnidosCell Press2016-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/60625Swinstead, Erin E.; Miranda, Tina B.; Paakinaho, Ville; Baek, Songjoon; Goldstein, Ido; et al.; Steroid Receptors Reprogram FoxA1 Occupancy through Dynamic Chromatin Transitions; Cell Press; Cell; 165; 3; 4-2016; 593-6050092-8674CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cell.2016.02.067info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0092867416302574info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:15Zoai:ri.conicet.gov.ar:11336/60625instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:15.704CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Steroid Receptors Reprogram FoxA1 Occupancy through Dynamic Chromatin Transitions
title Steroid Receptors Reprogram FoxA1 Occupancy through Dynamic Chromatin Transitions
spellingShingle Steroid Receptors Reprogram FoxA1 Occupancy through Dynamic Chromatin Transitions
Swinstead, Erin E.
Foxa1
Chromatin
Single Molecule Tracking
Glucocorticoid Receptor
title_short Steroid Receptors Reprogram FoxA1 Occupancy through Dynamic Chromatin Transitions
title_full Steroid Receptors Reprogram FoxA1 Occupancy through Dynamic Chromatin Transitions
title_fullStr Steroid Receptors Reprogram FoxA1 Occupancy through Dynamic Chromatin Transitions
title_full_unstemmed Steroid Receptors Reprogram FoxA1 Occupancy through Dynamic Chromatin Transitions
title_sort Steroid Receptors Reprogram FoxA1 Occupancy through Dynamic Chromatin Transitions
dc.creator.none.fl_str_mv Swinstead, Erin E.
Miranda, Tina B.
Paakinaho, Ville
Baek, Songjoon
Goldstein, Ido
Hawkins, Mary
Karpova, Tatiana S.
Ball, David
Mazza, Davide
Lavis, Luke D.
Grimm, Jonathan B.
Morisaki, Tatsuya
Grøntved, Lars
Presman, Diego Martin
Hager, Gordon L.
author Swinstead, Erin E.
author_facet Swinstead, Erin E.
Miranda, Tina B.
Paakinaho, Ville
Baek, Songjoon
Goldstein, Ido
Hawkins, Mary
Karpova, Tatiana S.
Ball, David
Mazza, Davide
Lavis, Luke D.
Grimm, Jonathan B.
Morisaki, Tatsuya
Grøntved, Lars
Presman, Diego Martin
Hager, Gordon L.
author_role author
author2 Miranda, Tina B.
Paakinaho, Ville
Baek, Songjoon
Goldstein, Ido
Hawkins, Mary
Karpova, Tatiana S.
Ball, David
Mazza, Davide
Lavis, Luke D.
Grimm, Jonathan B.
Morisaki, Tatsuya
Grøntved, Lars
Presman, Diego Martin
Hager, Gordon L.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Foxa1
Chromatin
Single Molecule Tracking
Glucocorticoid Receptor
topic Foxa1
Chromatin
Single Molecule Tracking
Glucocorticoid Receptor
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The estrogen receptor (ER), glucocorticoid receptor (GR), and forkhead box protein 1 (FoxA1) are significant factors in breast cancer progression. FoxA1 has been implicated in establishing ER-binding patterns though its unique ability to serve as a pioneer factor. However, the molecular interplay between ER, GR, and FoxA1 requires further investigation. Here we show that ER and GR both have the ability to alter the genomic distribution of the FoxA1 pioneer factor. Single-molecule tracking experiments in live cells reveal a highly dynamic interaction of FoxA1 with chromatin in vivo. Furthermore, the FoxA1 factor is not associated with detectable footprints at its binding sites throughout the genome. These findings support a model wherein interactions between transcription factors and pioneer factors are highly dynamic. Moreover, at a subset of genomic sites, the role of pioneer can be reversed, with the steroid receptors serving to enhance binding of FoxA1.
Fil: Swinstead, Erin E.. National Institutes of Health; Estados Unidos
Fil: Miranda, Tina B.. National Institutes of Health; Estados Unidos
Fil: Paakinaho, Ville. National Institutes of Health; Estados Unidos
Fil: Baek, Songjoon. National Institutes of Health; Estados Unidos
Fil: Goldstein, Ido. National Institutes of Health; Estados Unidos
Fil: Hawkins, Mary. National Institutes of Health; Estados Unidos
Fil: Karpova, Tatiana S.. National Institutes of Health; Estados Unidos
Fil: Ball, David. National Institutes of Health; Estados Unidos
Fil: Mazza, Davide. Universita Vita-salute San Raffaele; Italia
Fil: Lavis, Luke D.. Howard Hughes Medical Institute; Estados Unidos
Fil: Grimm, Jonathan B.. Howard Hughes Medical Institute; Estados Unidos
Fil: Morisaki, Tatsuya. National Institutes of Health; Estados Unidos. State University of Colorado - Fort Collins; Estados Unidos
Fil: Grøntved, Lars. National Institutes of Health; Estados Unidos
Fil: Presman, Diego Martin. National Institutes of Health; Estados Unidos
Fil: Hager, Gordon L.. National Institutes of Health; Estados Unidos
description The estrogen receptor (ER), glucocorticoid receptor (GR), and forkhead box protein 1 (FoxA1) are significant factors in breast cancer progression. FoxA1 has been implicated in establishing ER-binding patterns though its unique ability to serve as a pioneer factor. However, the molecular interplay between ER, GR, and FoxA1 requires further investigation. Here we show that ER and GR both have the ability to alter the genomic distribution of the FoxA1 pioneer factor. Single-molecule tracking experiments in live cells reveal a highly dynamic interaction of FoxA1 with chromatin in vivo. Furthermore, the FoxA1 factor is not associated with detectable footprints at its binding sites throughout the genome. These findings support a model wherein interactions between transcription factors and pioneer factors are highly dynamic. Moreover, at a subset of genomic sites, the role of pioneer can be reversed, with the steroid receptors serving to enhance binding of FoxA1.
publishDate 2016
dc.date.none.fl_str_mv 2016-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/60625
Swinstead, Erin E.; Miranda, Tina B.; Paakinaho, Ville; Baek, Songjoon; Goldstein, Ido; et al.; Steroid Receptors Reprogram FoxA1 Occupancy through Dynamic Chromatin Transitions; Cell Press; Cell; 165; 3; 4-2016; 593-605
0092-8674
CONICET Digital
CONICET
url http://hdl.handle.net/11336/60625
identifier_str_mv Swinstead, Erin E.; Miranda, Tina B.; Paakinaho, Ville; Baek, Songjoon; Goldstein, Ido; et al.; Steroid Receptors Reprogram FoxA1 Occupancy through Dynamic Chromatin Transitions; Cell Press; Cell; 165; 3; 4-2016; 593-605
0092-8674
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cell.2016.02.067
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0092867416302574
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269746589859840
score 13.13397

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