Suppression of ethylene perception after exposure to cooling conditions delays the progress of softening in 'Hayward' kiwifruit

Autores
Ilina, Natalia; Alem, Hubert J.; Pagano, Eduardo Antonio; Sozzi, Gabriel Oscar
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To investigate the physiological effect of ethylene and 1-methylcyclopropene (1-MCP) in the progress of 'Hayward' kiwifruit softening, fruit were treated with ethylene or 1-MCP right after harvest, or with 1-MCP at different ripening stages after 40, 80 or 120 d of cold (0 °C) storage. Treatment with ethylene right after harvest stimulated flesh softening, increased and advanced the ethylene production peak and the expression of the genes KWACS1 and KWACO1 involved in ethylene biosynthesis. In contrast, treatment with 1-MCP at the same stage markedly retarded softening, and inhibited ethylene production. Ripening-related increases in KWACS1 and KWACO1 transcript abundance were largely blocked by 1-MCP treatment thus indicating that these genes are positively regulated by ethylene. Nevertheless, ethylene and 1-MCP effect was highly dependent on the growing region that may influence kiwifruit constitution, quality, storage potential and subsequent responsiveness to postharvest technologies. Kiwifruit stored for 40, 80 or 120 d of cold storage and then treated with 1-MCP before rewarming to 20 °C for further ripening displayed a reduced flesh softening rate and an extended 'eating ripe' stage. These results clearly indicate that the application of 1-MCP can play a significant role in both the initiation and progress of the kiwifruit softening process. 1-MCP inhibited or severely decreased autocatalytic ethylene production at every ripening stage. KWACS1 and KWACO1 gene transcription was inhibited by 1-MCP treatment after 40 and 80 d of cold storage thus suggesting that there is a positive feedback regulation for ethylene production even after cold storage. In contrast, KWACS2 transcript levels did not show a clear response to 1-MCP in every experiment. This work provides evidence that kiwifruit softening can be delayed by inhibiting ethylene perception, even when fruit have reached advanced stages of ripening. These findings point to the commercial usefulness of 1-MCP in 'Hayward' kiwifruit postharvest conservation and consumer acceptance.
Fil: Ilina, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Alem, Hubert J.. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Pagano, Eduardo Antonio. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Sozzi, Gabriel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Materia
1-Aminocyclopropane-1-Carboxylic Acid Synthase And Oxidase Gene Expression
1-Methylcyclopropene
Actinidia Deliciosa
Cold Storage
Ethylene
Firmness
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/61001
Acceder
id CONICETDig_5c8e3d0ce0274fbbc9031897efcc045e
oai_identifier_str oai:ri.conicet.gov.ar:11336/61001
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Suppression of ethylene perception after exposure to cooling conditions delays the progress of softening in 'Hayward' kiwifruitIlina, NataliaAlem, Hubert J.Pagano, Eduardo AntonioSozzi, Gabriel Oscar1-Aminocyclopropane-1-Carboxylic Acid Synthase And Oxidase Gene Expression1-MethylcyclopropeneActinidia DeliciosaCold StorageEthyleneFirmnesshttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4To investigate the physiological effect of ethylene and 1-methylcyclopropene (1-MCP) in the progress of 'Hayward' kiwifruit softening, fruit were treated with ethylene or 1-MCP right after harvest, or with 1-MCP at different ripening stages after 40, 80 or 120 d of cold (0 °C) storage. Treatment with ethylene right after harvest stimulated flesh softening, increased and advanced the ethylene production peak and the expression of the genes KWACS1 and KWACO1 involved in ethylene biosynthesis. In contrast, treatment with 1-MCP at the same stage markedly retarded softening, and inhibited ethylene production. Ripening-related increases in KWACS1 and KWACO1 transcript abundance were largely blocked by 1-MCP treatment thus indicating that these genes are positively regulated by ethylene. Nevertheless, ethylene and 1-MCP effect was highly dependent on the growing region that may influence kiwifruit constitution, quality, storage potential and subsequent responsiveness to postharvest technologies. Kiwifruit stored for 40, 80 or 120 d of cold storage and then treated with 1-MCP before rewarming to 20 °C for further ripening displayed a reduced flesh softening rate and an extended 'eating ripe' stage. These results clearly indicate that the application of 1-MCP can play a significant role in both the initiation and progress of the kiwifruit softening process. 1-MCP inhibited or severely decreased autocatalytic ethylene production at every ripening stage. KWACS1 and KWACO1 gene transcription was inhibited by 1-MCP treatment after 40 and 80 d of cold storage thus suggesting that there is a positive feedback regulation for ethylene production even after cold storage. In contrast, KWACS2 transcript levels did not show a clear response to 1-MCP in every experiment. This work provides evidence that kiwifruit softening can be delayed by inhibiting ethylene perception, even when fruit have reached advanced stages of ripening. These findings point to the commercial usefulness of 1-MCP in 'Hayward' kiwifruit postharvest conservation and consumer acceptance.Fil: Ilina, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaFil: Alem, Hubert J.. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Pagano, Eduardo Antonio. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Sozzi, Gabriel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; ArgentinaElsevier Science2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61001Ilina, Natalia; Alem, Hubert J.; Pagano, Eduardo Antonio; Sozzi, Gabriel Oscar; Suppression of ethylene perception after exposure to cooling conditions delays the progress of softening in 'Hayward' kiwifruit; Elsevier Science; Postharvest Biology and Technology; 55; 3; 3-2010; 160-1680925-5214CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0925521409002385info:eu-repo/semantics/altIdentifier/doi/10.1016/j.postharvbio.2009.11.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:58:25Zoai:ri.conicet.gov.ar:11336/61001instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:58:25.993CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Suppression of ethylene perception after exposure to cooling conditions delays the progress of softening in 'Hayward' kiwifruit
title Suppression of ethylene perception after exposure to cooling conditions delays the progress of softening in 'Hayward' kiwifruit
spellingShingle Suppression of ethylene perception after exposure to cooling conditions delays the progress of softening in 'Hayward' kiwifruit
Ilina, Natalia
1-Aminocyclopropane-1-Carboxylic Acid Synthase And Oxidase Gene Expression
1-Methylcyclopropene
Actinidia Deliciosa
Cold Storage
Ethylene
Firmness
title_short Suppression of ethylene perception after exposure to cooling conditions delays the progress of softening in 'Hayward' kiwifruit
title_full Suppression of ethylene perception after exposure to cooling conditions delays the progress of softening in 'Hayward' kiwifruit
title_fullStr Suppression of ethylene perception after exposure to cooling conditions delays the progress of softening in 'Hayward' kiwifruit
title_full_unstemmed Suppression of ethylene perception after exposure to cooling conditions delays the progress of softening in 'Hayward' kiwifruit
title_sort Suppression of ethylene perception after exposure to cooling conditions delays the progress of softening in 'Hayward' kiwifruit
dc.creator.none.fl_str_mv Ilina, Natalia
Alem, Hubert J.
Pagano, Eduardo Antonio
Sozzi, Gabriel Oscar
author Ilina, Natalia
author_facet Ilina, Natalia
Alem, Hubert J.
Pagano, Eduardo Antonio
Sozzi, Gabriel Oscar
author_role author
author2 Alem, Hubert J.
Pagano, Eduardo Antonio
Sozzi, Gabriel Oscar
author2_role author
author
author
dc.subject.none.fl_str_mv 1-Aminocyclopropane-1-Carboxylic Acid Synthase And Oxidase Gene Expression
1-Methylcyclopropene
Actinidia Deliciosa
Cold Storage
Ethylene
Firmness
topic 1-Aminocyclopropane-1-Carboxylic Acid Synthase And Oxidase Gene Expression
1-Methylcyclopropene
Actinidia Deliciosa
Cold Storage
Ethylene
Firmness
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv To investigate the physiological effect of ethylene and 1-methylcyclopropene (1-MCP) in the progress of 'Hayward' kiwifruit softening, fruit were treated with ethylene or 1-MCP right after harvest, or with 1-MCP at different ripening stages after 40, 80 or 120 d of cold (0 °C) storage. Treatment with ethylene right after harvest stimulated flesh softening, increased and advanced the ethylene production peak and the expression of the genes KWACS1 and KWACO1 involved in ethylene biosynthesis. In contrast, treatment with 1-MCP at the same stage markedly retarded softening, and inhibited ethylene production. Ripening-related increases in KWACS1 and KWACO1 transcript abundance were largely blocked by 1-MCP treatment thus indicating that these genes are positively regulated by ethylene. Nevertheless, ethylene and 1-MCP effect was highly dependent on the growing region that may influence kiwifruit constitution, quality, storage potential and subsequent responsiveness to postharvest technologies. Kiwifruit stored for 40, 80 or 120 d of cold storage and then treated with 1-MCP before rewarming to 20 °C for further ripening displayed a reduced flesh softening rate and an extended 'eating ripe' stage. These results clearly indicate that the application of 1-MCP can play a significant role in both the initiation and progress of the kiwifruit softening process. 1-MCP inhibited or severely decreased autocatalytic ethylene production at every ripening stage. KWACS1 and KWACO1 gene transcription was inhibited by 1-MCP treatment after 40 and 80 d of cold storage thus suggesting that there is a positive feedback regulation for ethylene production even after cold storage. In contrast, KWACS2 transcript levels did not show a clear response to 1-MCP in every experiment. This work provides evidence that kiwifruit softening can be delayed by inhibiting ethylene perception, even when fruit have reached advanced stages of ripening. These findings point to the commercial usefulness of 1-MCP in 'Hayward' kiwifruit postharvest conservation and consumer acceptance.
Fil: Ilina, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
Fil: Alem, Hubert J.. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Pagano, Eduardo Antonio. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Sozzi, Gabriel Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
description To investigate the physiological effect of ethylene and 1-methylcyclopropene (1-MCP) in the progress of 'Hayward' kiwifruit softening, fruit were treated with ethylene or 1-MCP right after harvest, or with 1-MCP at different ripening stages after 40, 80 or 120 d of cold (0 °C) storage. Treatment with ethylene right after harvest stimulated flesh softening, increased and advanced the ethylene production peak and the expression of the genes KWACS1 and KWACO1 involved in ethylene biosynthesis. In contrast, treatment with 1-MCP at the same stage markedly retarded softening, and inhibited ethylene production. Ripening-related increases in KWACS1 and KWACO1 transcript abundance were largely blocked by 1-MCP treatment thus indicating that these genes are positively regulated by ethylene. Nevertheless, ethylene and 1-MCP effect was highly dependent on the growing region that may influence kiwifruit constitution, quality, storage potential and subsequent responsiveness to postharvest technologies. Kiwifruit stored for 40, 80 or 120 d of cold storage and then treated with 1-MCP before rewarming to 20 °C for further ripening displayed a reduced flesh softening rate and an extended 'eating ripe' stage. These results clearly indicate that the application of 1-MCP can play a significant role in both the initiation and progress of the kiwifruit softening process. 1-MCP inhibited or severely decreased autocatalytic ethylene production at every ripening stage. KWACS1 and KWACO1 gene transcription was inhibited by 1-MCP treatment after 40 and 80 d of cold storage thus suggesting that there is a positive feedback regulation for ethylene production even after cold storage. In contrast, KWACS2 transcript levels did not show a clear response to 1-MCP in every experiment. This work provides evidence that kiwifruit softening can be delayed by inhibiting ethylene perception, even when fruit have reached advanced stages of ripening. These findings point to the commercial usefulness of 1-MCP in 'Hayward' kiwifruit postharvest conservation and consumer acceptance.
publishDate 2010
dc.date.none.fl_str_mv 2010-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/61001
Ilina, Natalia; Alem, Hubert J.; Pagano, Eduardo Antonio; Sozzi, Gabriel Oscar; Suppression of ethylene perception after exposure to cooling conditions delays the progress of softening in 'Hayward' kiwifruit; Elsevier Science; Postharvest Biology and Technology; 55; 3; 3-2010; 160-168
0925-5214
CONICET Digital
CONICET
url http://hdl.handle.net/11336/61001
identifier_str_mv Ilina, Natalia; Alem, Hubert J.; Pagano, Eduardo Antonio; Sozzi, Gabriel Oscar; Suppression of ethylene perception after exposure to cooling conditions delays the progress of softening in 'Hayward' kiwifruit; Elsevier Science; Postharvest Biology and Technology; 55; 3; 3-2010; 160-168
0925-5214
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0925521409002385
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.postharvbio.2009.11.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083124664991744
score 13.22299

Publicaciones similares