Molecular characterization and interactome analysis of Trypanosoma cruzi Tryparedoxin 1

Autores
Piñeyro, María Dolores; Parodi Talice, Adriana; Portela, Magdalena; Arias, Diego Gustavo; Guerrero, Sergio Adrian; Robello, Carlos
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma cruzi tryparedoxin 1 (TcTXN1) is an oxidoreductase belonging to the thioredoxin superfamily, which mediates electron transfer between trypanothione and peroxiredoxins. In trypanosomes TXNs, and not thioredoxins, constitute the oxido-reductases of peroxiredoxins. Since, to date, there is no information concerning TcTXN1 substrates in T. cruzi, the aim of this work was to characterize TcTXN1 in two aspects: expression throughout T. cruzi life cycle and subcellular localization; and the study of TcTXN1 interacting-proteins. We demonstrate that TcTXN1 is a cytosolic and constitutively expressed protein in T. cruzi. In order to start to unravel the redox interactome of T. cruzi we designed an active site mutant protein lacking the resolving cysteine, and validated the complex formation in vitro between the mutated TcTXN1 and a known partner, the cytosolic peroxiredoxin. Through the expression of this mutant protein in parasites with an additional 6xHis-tag, heterodisulfide complexes were isolated by affinity chromatography and identified by 2-DE/MS. This allowed us to identify fifteen TcTXN1 proteins which are involved in two main processes: oxidative metabolism and protein synthesis and degradation. Our approach led us to the discovery of several putatively TcTXN1-interacting proteins thereby contributing to our understanding of the redox interactome of T. cruzi.
Fil: Piñeyro, María Dolores. Universidad de la Republica Facultad de Medicina; Uruguay. Instituto Pasteur de Montevideo; Uruguay
Fil: Parodi Talice, Adriana. Universidad de la Republica; Uruguay. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; Uruguay
Fil: Portela, Magdalena. Universidad de la República; Uruguay. Universidad de la Republica; Uruguay
Fil: Arias, Diego Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Robello, Carlos. Instituto Pasteur de Montevideo; Uruguay. Universidad de la Republica Facultad de Medicina; Uruguay
Materia
REDOX INTERACTOME
TRYPANOSOMA CRUZI
TRYPAREDOXIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/114002
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular characterization and interactome analysis of Trypanosoma cruzi Tryparedoxin 1Piñeyro, María DoloresParodi Talice, AdrianaPortela, MagdalenaArias, Diego GustavoGuerrero, Sergio AdrianRobello, CarlosREDOX INTERACTOMETRYPANOSOMA CRUZITRYPAREDOXINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma cruzi tryparedoxin 1 (TcTXN1) is an oxidoreductase belonging to the thioredoxin superfamily, which mediates electron transfer between trypanothione and peroxiredoxins. In trypanosomes TXNs, and not thioredoxins, constitute the oxido-reductases of peroxiredoxins. Since, to date, there is no information concerning TcTXN1 substrates in T. cruzi, the aim of this work was to characterize TcTXN1 in two aspects: expression throughout T. cruzi life cycle and subcellular localization; and the study of TcTXN1 interacting-proteins. We demonstrate that TcTXN1 is a cytosolic and constitutively expressed protein in T. cruzi. In order to start to unravel the redox interactome of T. cruzi we designed an active site mutant protein lacking the resolving cysteine, and validated the complex formation in vitro between the mutated TcTXN1 and a known partner, the cytosolic peroxiredoxin. Through the expression of this mutant protein in parasites with an additional 6xHis-tag, heterodisulfide complexes were isolated by affinity chromatography and identified by 2-DE/MS. This allowed us to identify fifteen TcTXN1 proteins which are involved in two main processes: oxidative metabolism and protein synthesis and degradation. Our approach led us to the discovery of several putatively TcTXN1-interacting proteins thereby contributing to our understanding of the redox interactome of T. cruzi.Fil: Piñeyro, María Dolores. Universidad de la Republica Facultad de Medicina; Uruguay. Instituto Pasteur de Montevideo; UruguayFil: Parodi Talice, Adriana. Universidad de la Republica; Uruguay. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; UruguayFil: Portela, Magdalena. Universidad de la República; Uruguay. Universidad de la Republica; UruguayFil: Arias, Diego Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Robello, Carlos. Instituto Pasteur de Montevideo; Uruguay. Universidad de la Republica Facultad de Medicina; UruguayElsevier Science2011-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/114002Piñeyro, María Dolores; Parodi Talice, Adriana; Portela, Magdalena; Arias, Diego Gustavo; Guerrero, Sergio Adrian; et al.; Molecular characterization and interactome analysis of Trypanosoma cruzi Tryparedoxin 1; Elsevier Science; Journal Of Proteomics; 74; 9; 8-2011; 1683-16921874-3919CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.elsevier.com/wps/find/journaleditorialboard.cws_home/713351/editorialboardinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jprot.2011.04.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:41:29Zoai:ri.conicet.gov.ar:11336/114002instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:41:29.975CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular characterization and interactome analysis of Trypanosoma cruzi Tryparedoxin 1
title Molecular characterization and interactome analysis of Trypanosoma cruzi Tryparedoxin 1
spellingShingle Molecular characterization and interactome analysis of Trypanosoma cruzi Tryparedoxin 1
Piñeyro, María Dolores
REDOX INTERACTOME
TRYPANOSOMA CRUZI
TRYPAREDOXIN
title_short Molecular characterization and interactome analysis of Trypanosoma cruzi Tryparedoxin 1
title_full Molecular characterization and interactome analysis of Trypanosoma cruzi Tryparedoxin 1
title_fullStr Molecular characterization and interactome analysis of Trypanosoma cruzi Tryparedoxin 1
title_full_unstemmed Molecular characterization and interactome analysis of Trypanosoma cruzi Tryparedoxin 1
title_sort Molecular characterization and interactome analysis of Trypanosoma cruzi Tryparedoxin 1
dc.creator.none.fl_str_mv Piñeyro, María Dolores
Parodi Talice, Adriana
Portela, Magdalena
Arias, Diego Gustavo
Guerrero, Sergio Adrian
Robello, Carlos
author Piñeyro, María Dolores
author_facet Piñeyro, María Dolores
Parodi Talice, Adriana
Portela, Magdalena
Arias, Diego Gustavo
Guerrero, Sergio Adrian
Robello, Carlos
author_role author
author2 Parodi Talice, Adriana
Portela, Magdalena
Arias, Diego Gustavo
Guerrero, Sergio Adrian
Robello, Carlos
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv REDOX INTERACTOME
TRYPANOSOMA CRUZI
TRYPAREDOXIN
topic REDOX INTERACTOME
TRYPANOSOMA CRUZI
TRYPAREDOXIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trypanosoma cruzi tryparedoxin 1 (TcTXN1) is an oxidoreductase belonging to the thioredoxin superfamily, which mediates electron transfer between trypanothione and peroxiredoxins. In trypanosomes TXNs, and not thioredoxins, constitute the oxido-reductases of peroxiredoxins. Since, to date, there is no information concerning TcTXN1 substrates in T. cruzi, the aim of this work was to characterize TcTXN1 in two aspects: expression throughout T. cruzi life cycle and subcellular localization; and the study of TcTXN1 interacting-proteins. We demonstrate that TcTXN1 is a cytosolic and constitutively expressed protein in T. cruzi. In order to start to unravel the redox interactome of T. cruzi we designed an active site mutant protein lacking the resolving cysteine, and validated the complex formation in vitro between the mutated TcTXN1 and a known partner, the cytosolic peroxiredoxin. Through the expression of this mutant protein in parasites with an additional 6xHis-tag, heterodisulfide complexes were isolated by affinity chromatography and identified by 2-DE/MS. This allowed us to identify fifteen TcTXN1 proteins which are involved in two main processes: oxidative metabolism and protein synthesis and degradation. Our approach led us to the discovery of several putatively TcTXN1-interacting proteins thereby contributing to our understanding of the redox interactome of T. cruzi.
Fil: Piñeyro, María Dolores. Universidad de la Republica Facultad de Medicina; Uruguay. Instituto Pasteur de Montevideo; Uruguay
Fil: Parodi Talice, Adriana. Universidad de la Republica; Uruguay. Universidad de la República; Uruguay. Instituto Pasteur de Montevideo; Uruguay
Fil: Portela, Magdalena. Universidad de la República; Uruguay. Universidad de la Republica; Uruguay
Fil: Arias, Diego Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Robello, Carlos. Instituto Pasteur de Montevideo; Uruguay. Universidad de la Republica Facultad de Medicina; Uruguay
description Trypanosoma cruzi tryparedoxin 1 (TcTXN1) is an oxidoreductase belonging to the thioredoxin superfamily, which mediates electron transfer between trypanothione and peroxiredoxins. In trypanosomes TXNs, and not thioredoxins, constitute the oxido-reductases of peroxiredoxins. Since, to date, there is no information concerning TcTXN1 substrates in T. cruzi, the aim of this work was to characterize TcTXN1 in two aspects: expression throughout T. cruzi life cycle and subcellular localization; and the study of TcTXN1 interacting-proteins. We demonstrate that TcTXN1 is a cytosolic and constitutively expressed protein in T. cruzi. In order to start to unravel the redox interactome of T. cruzi we designed an active site mutant protein lacking the resolving cysteine, and validated the complex formation in vitro between the mutated TcTXN1 and a known partner, the cytosolic peroxiredoxin. Through the expression of this mutant protein in parasites with an additional 6xHis-tag, heterodisulfide complexes were isolated by affinity chromatography and identified by 2-DE/MS. This allowed us to identify fifteen TcTXN1 proteins which are involved in two main processes: oxidative metabolism and protein synthesis and degradation. Our approach led us to the discovery of several putatively TcTXN1-interacting proteins thereby contributing to our understanding of the redox interactome of T. cruzi.
publishDate 2011
dc.date.none.fl_str_mv 2011-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/114002
Piñeyro, María Dolores; Parodi Talice, Adriana; Portela, Magdalena; Arias, Diego Gustavo; Guerrero, Sergio Adrian; et al.; Molecular characterization and interactome analysis of Trypanosoma cruzi Tryparedoxin 1; Elsevier Science; Journal Of Proteomics; 74; 9; 8-2011; 1683-1692
1874-3919
CONICET Digital
CONICET
url http://hdl.handle.net/11336/114002
identifier_str_mv Piñeyro, María Dolores; Parodi Talice, Adriana; Portela, Magdalena; Arias, Diego Gustavo; Guerrero, Sergio Adrian; et al.; Molecular characterization and interactome analysis of Trypanosoma cruzi Tryparedoxin 1; Elsevier Science; Journal Of Proteomics; 74; 9; 8-2011; 1683-1692
1874-3919
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.elsevier.com/wps/find/journaleditorialboard.cws_home/713351/editorialboard
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jprot.2011.04.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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