Redox metabolism in Trypanosoma cruzi: functional characterization of tryparedoxins revisited

Autores
Arias, Diego Gustavo; Márquez, Vanina Elizabet; Chiribao, Maria L.; Gadhela, Fernanda R.; Robello, Carlos; Iglesias, Alberto Alvaro; Guerrero, Sergio Adrian
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tryparedoxins (TXNs) are multipurpose oxidoreductases from trypanosomatids that transfer reducing equivalents from trypanothione to different thiol proteins. In Trypanosoma cruzi, two genes coding for TXN like proteins have been identified: TXNI, previously characterized as a cytoplasmic protein, and TXNII, a putative tail-anchored membrane protein. In this work, we performed a comparative functional characterization of T. cruzi TXNs. Particularly, we cloned the gene region coding for the soluble version of TXNII for its heterologous expression. The truncated recombinant protein (without its 22 C-terminal transmenbrane amino acids) showed TXN activity. It was also able to transfer reducing equivalents from trypanothione, glutathione or dihydrolipoamide to different acceptors, including methionine sulfoxide reductases and peroxiredoxins. Results support the occurrence and functionality of a second tryparedoxin, which appears as a new component in redox scenario in T. cruzi.
Fil: Arias, Diego Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Márquez, Vanina Elizabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Chiribao, Maria L.. Universidad de la República; Uruguay
Fil: Gadhela, Fernanda R.. Universidade Estadual Do Campinas. Instituto de Biología; Brasil
Fil: Robello, Carlos. Universidad de la República; Uruguay
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Materia
Trypanosoma
Tryparedoxin
Trypanothione
Ros
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23469
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/23469
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Redox metabolism in Trypanosoma cruzi: functional characterization of tryparedoxins revisitedArias, Diego GustavoMárquez, Vanina ElizabetChiribao, Maria L.Gadhela, Fernanda R.Robello, CarlosIglesias, Alberto AlvaroGuerrero, Sergio AdrianTrypanosomaTryparedoxinTrypanothioneRoshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Tryparedoxins (TXNs) are multipurpose oxidoreductases from trypanosomatids that transfer reducing equivalents from trypanothione to different thiol proteins. In Trypanosoma cruzi, two genes coding for TXN like proteins have been identified: TXNI, previously characterized as a cytoplasmic protein, and TXNII, a putative tail-anchored membrane protein. In this work, we performed a comparative functional characterization of T. cruzi TXNs. Particularly, we cloned the gene region coding for the soluble version of TXNII for its heterologous expression. The truncated recombinant protein (without its 22 C-terminal transmenbrane amino acids) showed TXN activity. It was also able to transfer reducing equivalents from trypanothione, glutathione or dihydrolipoamide to different acceptors, including methionine sulfoxide reductases and peroxiredoxins. Results support the occurrence and functionality of a second tryparedoxin, which appears as a new component in redox scenario in T. cruzi.Fil: Arias, Diego Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Márquez, Vanina Elizabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Chiribao, Maria L.. Universidad de la República; UruguayFil: Gadhela, Fernanda R.. Universidade Estadual Do Campinas. Instituto de Biología; BrasilFil: Robello, Carlos. Universidad de la República; UruguayFil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaElsevier2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23469Arias, Diego Gustavo; Márquez, Vanina Elizabet; Chiribao, Maria L.; Gadhela, Fernanda R.; Robello, Carlos; et al.; Redox metabolism in Trypanosoma cruzi: functional characterization of tryparedoxins revisited; Elsevier; Free Radical Biology and Medicine; 63; 10-2013; 65-770891-5849CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0891584913002050info:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2013.04.036info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:00:56Zoai:ri.conicet.gov.ar:11336/23469instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:00:56.86CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Redox metabolism in Trypanosoma cruzi: functional characterization of tryparedoxins revisited
title Redox metabolism in Trypanosoma cruzi: functional characterization of tryparedoxins revisited
spellingShingle Redox metabolism in Trypanosoma cruzi: functional characterization of tryparedoxins revisited
Arias, Diego Gustavo
Trypanosoma
Tryparedoxin
Trypanothione
Ros
title_short Redox metabolism in Trypanosoma cruzi: functional characterization of tryparedoxins revisited
title_full Redox metabolism in Trypanosoma cruzi: functional characterization of tryparedoxins revisited
title_fullStr Redox metabolism in Trypanosoma cruzi: functional characterization of tryparedoxins revisited
title_full_unstemmed Redox metabolism in Trypanosoma cruzi: functional characterization of tryparedoxins revisited
title_sort Redox metabolism in Trypanosoma cruzi: functional characterization of tryparedoxins revisited
dc.creator.none.fl_str_mv Arias, Diego Gustavo
Márquez, Vanina Elizabet
Chiribao, Maria L.
Gadhela, Fernanda R.
Robello, Carlos
Iglesias, Alberto Alvaro
Guerrero, Sergio Adrian
author Arias, Diego Gustavo
author_facet Arias, Diego Gustavo
Márquez, Vanina Elizabet
Chiribao, Maria L.
Gadhela, Fernanda R.
Robello, Carlos
Iglesias, Alberto Alvaro
Guerrero, Sergio Adrian
author_role author
author2 Márquez, Vanina Elizabet
Chiribao, Maria L.
Gadhela, Fernanda R.
Robello, Carlos
Iglesias, Alberto Alvaro
Guerrero, Sergio Adrian
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Trypanosoma
Tryparedoxin
Trypanothione
Ros
topic Trypanosoma
Tryparedoxin
Trypanothione
Ros
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Tryparedoxins (TXNs) are multipurpose oxidoreductases from trypanosomatids that transfer reducing equivalents from trypanothione to different thiol proteins. In Trypanosoma cruzi, two genes coding for TXN like proteins have been identified: TXNI, previously characterized as a cytoplasmic protein, and TXNII, a putative tail-anchored membrane protein. In this work, we performed a comparative functional characterization of T. cruzi TXNs. Particularly, we cloned the gene region coding for the soluble version of TXNII for its heterologous expression. The truncated recombinant protein (without its 22 C-terminal transmenbrane amino acids) showed TXN activity. It was also able to transfer reducing equivalents from trypanothione, glutathione or dihydrolipoamide to different acceptors, including methionine sulfoxide reductases and peroxiredoxins. Results support the occurrence and functionality of a second tryparedoxin, which appears as a new component in redox scenario in T. cruzi.
Fil: Arias, Diego Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Márquez, Vanina Elizabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Chiribao, Maria L.. Universidad de la República; Uruguay
Fil: Gadhela, Fernanda R.. Universidade Estadual Do Campinas. Instituto de Biología; Brasil
Fil: Robello, Carlos. Universidad de la República; Uruguay
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
description Tryparedoxins (TXNs) are multipurpose oxidoreductases from trypanosomatids that transfer reducing equivalents from trypanothione to different thiol proteins. In Trypanosoma cruzi, two genes coding for TXN like proteins have been identified: TXNI, previously characterized as a cytoplasmic protein, and TXNII, a putative tail-anchored membrane protein. In this work, we performed a comparative functional characterization of T. cruzi TXNs. Particularly, we cloned the gene region coding for the soluble version of TXNII for its heterologous expression. The truncated recombinant protein (without its 22 C-terminal transmenbrane amino acids) showed TXN activity. It was also able to transfer reducing equivalents from trypanothione, glutathione or dihydrolipoamide to different acceptors, including methionine sulfoxide reductases and peroxiredoxins. Results support the occurrence and functionality of a second tryparedoxin, which appears as a new component in redox scenario in T. cruzi.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23469
Arias, Diego Gustavo; Márquez, Vanina Elizabet; Chiribao, Maria L.; Gadhela, Fernanda R.; Robello, Carlos; et al.; Redox metabolism in Trypanosoma cruzi: functional characterization of tryparedoxins revisited; Elsevier; Free Radical Biology and Medicine; 63; 10-2013; 65-77
0891-5849
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23469
identifier_str_mv Arias, Diego Gustavo; Márquez, Vanina Elizabet; Chiribao, Maria L.; Gadhela, Fernanda R.; Robello, Carlos; et al.; Redox metabolism in Trypanosoma cruzi: functional characterization of tryparedoxins revisited; Elsevier; Free Radical Biology and Medicine; 63; 10-2013; 65-77
0891-5849
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0891584913002050
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.freeradbiomed.2013.04.036
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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