On the occurrence of thioredoxin in Trypanosoma cruzi

Autores
Piattoni, Claudia Vanesa; Blancato, Victor Sebastian; Miglietta, Hilario Francisco; Iglesias, Alberto Alvaro; Guerrero, Sergio Adrian
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The full coding sequence for thioredoxin from Trypanosoma cruzi (TcTRX) strain Tulahuen O has been cloned into the pRSETA vector. The protein was expressed in Escherichia coli with an N-terminal extension of six histidine residues for purification through metal ion chromatography. The biological activity of recombinant TcTRX was proved utilizing the insulin reduction assay. Amino acid sequence alignment indicates a high identity of TcTRX with thioredoxins from different sources. Immunocytochemistry assays showed that TcTRX is present in epimastigote forms of T. cruzi, thus, indicating that the gene is expressed in vivo, rather than being a pseudogene. The in vivo occurrence of TcTRX points out the necessity of considering this protein as a molecular component of the redox metabolism in trypanosomatids.
Fil: Piattoni, Claudia Vanesa. Universidad Nacional del Litoral; Argentina
Fil: Blancato, Victor Sebastian. Universidad Nacional del Litoral; Argentina
Fil: Miglietta, Hilario. Universidad Nacional del Litoral; Argentina
Fil: Iglesias, Alberto Alvaro. Universidad Nacional del Litoral; Argentina
Fil: Guerrero, Sergio Adrian. Universidad Nacional del Litoral; Argentina
Materia
REDOX METABOLISM
THIOREDOXIN
TRYPANOSOMA CRUZI
TRYPANOSOMATIDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93610
Acceder
id CONICETDig_5a04c470a00ce8f122f6d37ed49e73d4
oai_identifier_str oai:ri.conicet.gov.ar:11336/93610
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling On the occurrence of thioredoxin in Trypanosoma cruziPiattoni, Claudia VanesaBlancato, Victor SebastianMiglietta, Hilario FranciscoIglesias, Alberto AlvaroGuerrero, Sergio AdrianREDOX METABOLISMTHIOREDOXINTRYPANOSOMA CRUZITRYPANOSOMATIDShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The full coding sequence for thioredoxin from Trypanosoma cruzi (TcTRX) strain Tulahuen O has been cloned into the pRSETA vector. The protein was expressed in Escherichia coli with an N-terminal extension of six histidine residues for purification through metal ion chromatography. The biological activity of recombinant TcTRX was proved utilizing the insulin reduction assay. Amino acid sequence alignment indicates a high identity of TcTRX with thioredoxins from different sources. Immunocytochemistry assays showed that TcTRX is present in epimastigote forms of T. cruzi, thus, indicating that the gene is expressed in vivo, rather than being a pseudogene. The in vivo occurrence of TcTRX points out the necessity of considering this protein as a molecular component of the redox metabolism in trypanosomatids.Fil: Piattoni, Claudia Vanesa. Universidad Nacional del Litoral; ArgentinaFil: Blancato, Victor Sebastian. Universidad Nacional del Litoral; ArgentinaFil: Miglietta, Hilario. Universidad Nacional del Litoral; ArgentinaFil: Iglesias, Alberto Alvaro. Universidad Nacional del Litoral; ArgentinaFil: Guerrero, Sergio Adrian. Universidad Nacional del Litoral; ArgentinaElsevier Science2006-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93610Piattoni, Claudia Vanesa; Blancato, Victor Sebastian; Miglietta, Hilario Francisco; Iglesias, Alberto Alvaro; Guerrero, Sergio Adrian; On the occurrence of thioredoxin in Trypanosoma cruzi; Elsevier Science; Acta Tropica; 97; 2; 2-2006; 151-1600001-706XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/doi:10.1016/j.actatropica.2005.10.005info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0001706X0500327Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:20Zoai:ri.conicet.gov.ar:11336/93610instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:21.064CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv On the occurrence of thioredoxin in Trypanosoma cruzi
title On the occurrence of thioredoxin in Trypanosoma cruzi
spellingShingle On the occurrence of thioredoxin in Trypanosoma cruzi
Piattoni, Claudia Vanesa
REDOX METABOLISM
THIOREDOXIN
TRYPANOSOMA CRUZI
TRYPANOSOMATIDS
title_short On the occurrence of thioredoxin in Trypanosoma cruzi
title_full On the occurrence of thioredoxin in Trypanosoma cruzi
title_fullStr On the occurrence of thioredoxin in Trypanosoma cruzi
title_full_unstemmed On the occurrence of thioredoxin in Trypanosoma cruzi
title_sort On the occurrence of thioredoxin in Trypanosoma cruzi
dc.creator.none.fl_str_mv Piattoni, Claudia Vanesa
Blancato, Victor Sebastian
Miglietta, Hilario Francisco
Iglesias, Alberto Alvaro
Guerrero, Sergio Adrian
author Piattoni, Claudia Vanesa
author_facet Piattoni, Claudia Vanesa
Blancato, Victor Sebastian
Miglietta, Hilario Francisco
Iglesias, Alberto Alvaro
Guerrero, Sergio Adrian
author_role author
author2 Blancato, Victor Sebastian
Miglietta, Hilario Francisco
Iglesias, Alberto Alvaro
Guerrero, Sergio Adrian
author2_role author
author
author
author
dc.subject.none.fl_str_mv REDOX METABOLISM
THIOREDOXIN
TRYPANOSOMA CRUZI
TRYPANOSOMATIDS
topic REDOX METABOLISM
THIOREDOXIN
TRYPANOSOMA CRUZI
TRYPANOSOMATIDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The full coding sequence for thioredoxin from Trypanosoma cruzi (TcTRX) strain Tulahuen O has been cloned into the pRSETA vector. The protein was expressed in Escherichia coli with an N-terminal extension of six histidine residues for purification through metal ion chromatography. The biological activity of recombinant TcTRX was proved utilizing the insulin reduction assay. Amino acid sequence alignment indicates a high identity of TcTRX with thioredoxins from different sources. Immunocytochemistry assays showed that TcTRX is present in epimastigote forms of T. cruzi, thus, indicating that the gene is expressed in vivo, rather than being a pseudogene. The in vivo occurrence of TcTRX points out the necessity of considering this protein as a molecular component of the redox metabolism in trypanosomatids.
Fil: Piattoni, Claudia Vanesa. Universidad Nacional del Litoral; Argentina
Fil: Blancato, Victor Sebastian. Universidad Nacional del Litoral; Argentina
Fil: Miglietta, Hilario. Universidad Nacional del Litoral; Argentina
Fil: Iglesias, Alberto Alvaro. Universidad Nacional del Litoral; Argentina
Fil: Guerrero, Sergio Adrian. Universidad Nacional del Litoral; Argentina
description The full coding sequence for thioredoxin from Trypanosoma cruzi (TcTRX) strain Tulahuen O has been cloned into the pRSETA vector. The protein was expressed in Escherichia coli with an N-terminal extension of six histidine residues for purification through metal ion chromatography. The biological activity of recombinant TcTRX was proved utilizing the insulin reduction assay. Amino acid sequence alignment indicates a high identity of TcTRX with thioredoxins from different sources. Immunocytochemistry assays showed that TcTRX is present in epimastigote forms of T. cruzi, thus, indicating that the gene is expressed in vivo, rather than being a pseudogene. The in vivo occurrence of TcTRX points out the necessity of considering this protein as a molecular component of the redox metabolism in trypanosomatids.
publishDate 2006
dc.date.none.fl_str_mv 2006-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93610
Piattoni, Claudia Vanesa; Blancato, Victor Sebastian; Miglietta, Hilario Francisco; Iglesias, Alberto Alvaro; Guerrero, Sergio Adrian; On the occurrence of thioredoxin in Trypanosoma cruzi; Elsevier Science; Acta Tropica; 97; 2; 2-2006; 151-160
0001-706X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93610
identifier_str_mv Piattoni, Claudia Vanesa; Blancato, Victor Sebastian; Miglietta, Hilario Francisco; Iglesias, Alberto Alvaro; Guerrero, Sergio Adrian; On the occurrence of thioredoxin in Trypanosoma cruzi; Elsevier Science; Acta Tropica; 97; 2; 2-2006; 151-160
0001-706X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/doi:10.1016/j.actatropica.2005.10.005
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0001706X0500327X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613578727882752
score 13.070432

Publicaciones similares