New Retinal Pigment Epithelial Cell Model to Unravel Neuroprotection Sensors of Neurodegeneration in Retinal Disease

Autores
Asatryan, Aram; Calandria, Jorgelina M.; Kautzmann, Marie-Audrey; Jun, Bokkyoo; Gordon, William C.; Do, Khanh V; Bhattacharjee, S.; Pham, Thang L.; Bermúdez, Vicente; Mateos, Melina Valeria; Heap, Jessica; Bazan, Nicolás G.
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Retinal pigment epithelial (RPE) cells sustain photoreceptor integrity, and when this function is disrupted, retinal degenerations ensue. Herein, we characterize a new cell line from human RPE that we termed ABC. These cells remarkably recapitulate human eye native cells. Distinctive from other epithelia, RPE cells originate from the neural crest and follow a neural development but are terminally differentiated into “epithelial” type, thus sharing characteristics with their neuronal lineages counterparts. Additionally, they form microvilli, tight junctions, and honeycomb packing and express distinctive markers. In these cells, outer segment phagocytosis, phagolysosome fate, phospholipid metabolism, and lipid mediator release can be studied. ABC cells display higher resistance to oxidative stress and are protected from senescence through mTOR inhibition, making them more stable in culture. The cells are responsive to Neuroprotectin D1 (NPD1), which downregulates inflammasomes and upregulates antioxidant and anti-inflammatory genes. ABC gene expression profile displays close proximity to native RPE lineage, making them a reliable cell system to unravel signaling in uncompensated oxidative stress (UOS) and retinal degenerative disease to define neuroprotection sites.
Fil: Asatryan, Aram. State University of Louisiana; Estados Unidos
Fil: Calandria, Jorgelina M.. State University of Louisiana; Estados Unidos
Fil: Kautzmann, Marie-Audrey. State University of Louisiana; Estados Unidos
Fil: Jun, Bokkyoo. State University of Louisiana; Estados Unidos
Fil: Gordon, William C.. State University of Louisiana; Estados Unidos
Fil: Do, Khanh V. State University of Louisiana; Estados Unidos
Fil: Bhattacharjee, S.. State University of Louisiana; Estados Unidos
Fil: Pham, Thang L.. State University of Louisiana; Estados Unidos
Fil: Bermúdez, Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Heap, Jessica. State University of Louisiana; Estados Unidos
Fil: Bazan, Nicolás G.. State University of Louisiana; Estados Unidos
Materia
AGE-RELATED MACULAR DEGENERATION (AMD)
APOPTOSIS
AUTOPHAGY
GENE EXPRESSION
LIPIDS
NEUROPROTECTIN D1
RPE CELL
SINGLE CELL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/202305
Acceder
id CONICETDig_5afb368fb88cf06a90453b6ed80ab0ce
oai_identifier_str oai:ri.conicet.gov.ar:11336/202305
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling New Retinal Pigment Epithelial Cell Model to Unravel Neuroprotection Sensors of Neurodegeneration in Retinal DiseaseAsatryan, AramCalandria, Jorgelina M.Kautzmann, Marie-AudreyJun, BokkyooGordon, William C.Do, Khanh VBhattacharjee, S.Pham, Thang L.Bermúdez, VicenteMateos, Melina ValeriaHeap, JessicaBazan, Nicolás G.AGE-RELATED MACULAR DEGENERATION (AMD)APOPTOSISAUTOPHAGYGENE EXPRESSIONLIPIDSNEUROPROTECTIN D1RPE CELLSINGLE CELLhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Retinal pigment epithelial (RPE) cells sustain photoreceptor integrity, and when this function is disrupted, retinal degenerations ensue. Herein, we characterize a new cell line from human RPE that we termed ABC. These cells remarkably recapitulate human eye native cells. Distinctive from other epithelia, RPE cells originate from the neural crest and follow a neural development but are terminally differentiated into “epithelial” type, thus sharing characteristics with their neuronal lineages counterparts. Additionally, they form microvilli, tight junctions, and honeycomb packing and express distinctive markers. In these cells, outer segment phagocytosis, phagolysosome fate, phospholipid metabolism, and lipid mediator release can be studied. ABC cells display higher resistance to oxidative stress and are protected from senescence through mTOR inhibition, making them more stable in culture. The cells are responsive to Neuroprotectin D1 (NPD1), which downregulates inflammasomes and upregulates antioxidant and anti-inflammatory genes. ABC gene expression profile displays close proximity to native RPE lineage, making them a reliable cell system to unravel signaling in uncompensated oxidative stress (UOS) and retinal degenerative disease to define neuroprotection sites.Fil: Asatryan, Aram. State University of Louisiana; Estados UnidosFil: Calandria, Jorgelina M.. State University of Louisiana; Estados UnidosFil: Kautzmann, Marie-Audrey. State University of Louisiana; Estados UnidosFil: Jun, Bokkyoo. State University of Louisiana; Estados UnidosFil: Gordon, William C.. State University of Louisiana; Estados UnidosFil: Do, Khanh V. State University of Louisiana; Estados UnidosFil: Bhattacharjee, S.. State University of Louisiana; Estados UnidosFil: Pham, Thang L.. State University of Louisiana; Estados UnidosFil: Bermúdez, Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Heap, Jessica. State University of Louisiana; Estados UnidosFil: Bazan, Nicolás G.. State University of Louisiana; Estados UnidosFrontiers Media2022-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/202305Asatryan, Aram; Calandria, Jorgelina M.; Kautzmann, Marie-Audrey; Jun, Bokkyoo; Gordon, William C.; et al.; New Retinal Pigment Epithelial Cell Model to Unravel Neuroprotection Sensors of Neurodegeneration in Retinal Disease; Frontiers Media; Frontiers in Neuroscience; 16; 926629; 30-6-2022; 1-181662-453XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fnins.2022.926629/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fnins.2022.926629info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:53Zoai:ri.conicet.gov.ar:11336/202305instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:53.732CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New Retinal Pigment Epithelial Cell Model to Unravel Neuroprotection Sensors of Neurodegeneration in Retinal Disease
title New Retinal Pigment Epithelial Cell Model to Unravel Neuroprotection Sensors of Neurodegeneration in Retinal Disease
spellingShingle New Retinal Pigment Epithelial Cell Model to Unravel Neuroprotection Sensors of Neurodegeneration in Retinal Disease
Asatryan, Aram
AGE-RELATED MACULAR DEGENERATION (AMD)
APOPTOSIS
AUTOPHAGY
GENE EXPRESSION
LIPIDS
NEUROPROTECTIN D1
RPE CELL
SINGLE CELL
title_short New Retinal Pigment Epithelial Cell Model to Unravel Neuroprotection Sensors of Neurodegeneration in Retinal Disease
title_full New Retinal Pigment Epithelial Cell Model to Unravel Neuroprotection Sensors of Neurodegeneration in Retinal Disease
title_fullStr New Retinal Pigment Epithelial Cell Model to Unravel Neuroprotection Sensors of Neurodegeneration in Retinal Disease
title_full_unstemmed New Retinal Pigment Epithelial Cell Model to Unravel Neuroprotection Sensors of Neurodegeneration in Retinal Disease
title_sort New Retinal Pigment Epithelial Cell Model to Unravel Neuroprotection Sensors of Neurodegeneration in Retinal Disease
dc.creator.none.fl_str_mv Asatryan, Aram
Calandria, Jorgelina M.
Kautzmann, Marie-Audrey
Jun, Bokkyoo
Gordon, William C.
Do, Khanh V
Bhattacharjee, S.
Pham, Thang L.
Bermúdez, Vicente
Mateos, Melina Valeria
Heap, Jessica
Bazan, Nicolás G.
author Asatryan, Aram
author_facet Asatryan, Aram
Calandria, Jorgelina M.
Kautzmann, Marie-Audrey
Jun, Bokkyoo
Gordon, William C.
Do, Khanh V
Bhattacharjee, S.
Pham, Thang L.
Bermúdez, Vicente
Mateos, Melina Valeria
Heap, Jessica
Bazan, Nicolás G.
author_role author
author2 Calandria, Jorgelina M.
Kautzmann, Marie-Audrey
Jun, Bokkyoo
Gordon, William C.
Do, Khanh V
Bhattacharjee, S.
Pham, Thang L.
Bermúdez, Vicente
Mateos, Melina Valeria
Heap, Jessica
Bazan, Nicolás G.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AGE-RELATED MACULAR DEGENERATION (AMD)
APOPTOSIS
AUTOPHAGY
GENE EXPRESSION
LIPIDS
NEUROPROTECTIN D1
RPE CELL
SINGLE CELL
topic AGE-RELATED MACULAR DEGENERATION (AMD)
APOPTOSIS
AUTOPHAGY
GENE EXPRESSION
LIPIDS
NEUROPROTECTIN D1
RPE CELL
SINGLE CELL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Retinal pigment epithelial (RPE) cells sustain photoreceptor integrity, and when this function is disrupted, retinal degenerations ensue. Herein, we characterize a new cell line from human RPE that we termed ABC. These cells remarkably recapitulate human eye native cells. Distinctive from other epithelia, RPE cells originate from the neural crest and follow a neural development but are terminally differentiated into “epithelial” type, thus sharing characteristics with their neuronal lineages counterparts. Additionally, they form microvilli, tight junctions, and honeycomb packing and express distinctive markers. In these cells, outer segment phagocytosis, phagolysosome fate, phospholipid metabolism, and lipid mediator release can be studied. ABC cells display higher resistance to oxidative stress and are protected from senescence through mTOR inhibition, making them more stable in culture. The cells are responsive to Neuroprotectin D1 (NPD1), which downregulates inflammasomes and upregulates antioxidant and anti-inflammatory genes. ABC gene expression profile displays close proximity to native RPE lineage, making them a reliable cell system to unravel signaling in uncompensated oxidative stress (UOS) and retinal degenerative disease to define neuroprotection sites.
Fil: Asatryan, Aram. State University of Louisiana; Estados Unidos
Fil: Calandria, Jorgelina M.. State University of Louisiana; Estados Unidos
Fil: Kautzmann, Marie-Audrey. State University of Louisiana; Estados Unidos
Fil: Jun, Bokkyoo. State University of Louisiana; Estados Unidos
Fil: Gordon, William C.. State University of Louisiana; Estados Unidos
Fil: Do, Khanh V. State University of Louisiana; Estados Unidos
Fil: Bhattacharjee, S.. State University of Louisiana; Estados Unidos
Fil: Pham, Thang L.. State University of Louisiana; Estados Unidos
Fil: Bermúdez, Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Mateos, Melina Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Heap, Jessica. State University of Louisiana; Estados Unidos
Fil: Bazan, Nicolás G.. State University of Louisiana; Estados Unidos
description Retinal pigment epithelial (RPE) cells sustain photoreceptor integrity, and when this function is disrupted, retinal degenerations ensue. Herein, we characterize a new cell line from human RPE that we termed ABC. These cells remarkably recapitulate human eye native cells. Distinctive from other epithelia, RPE cells originate from the neural crest and follow a neural development but are terminally differentiated into “epithelial” type, thus sharing characteristics with their neuronal lineages counterparts. Additionally, they form microvilli, tight junctions, and honeycomb packing and express distinctive markers. In these cells, outer segment phagocytosis, phagolysosome fate, phospholipid metabolism, and lipid mediator release can be studied. ABC cells display higher resistance to oxidative stress and are protected from senescence through mTOR inhibition, making them more stable in culture. The cells are responsive to Neuroprotectin D1 (NPD1), which downregulates inflammasomes and upregulates antioxidant and anti-inflammatory genes. ABC gene expression profile displays close proximity to native RPE lineage, making them a reliable cell system to unravel signaling in uncompensated oxidative stress (UOS) and retinal degenerative disease to define neuroprotection sites.
publishDate 2022
dc.date.none.fl_str_mv 2022-06-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/202305
Asatryan, Aram; Calandria, Jorgelina M.; Kautzmann, Marie-Audrey; Jun, Bokkyoo; Gordon, William C.; et al.; New Retinal Pigment Epithelial Cell Model to Unravel Neuroprotection Sensors of Neurodegeneration in Retinal Disease; Frontiers Media; Frontiers in Neuroscience; 16; 926629; 30-6-2022; 1-18
1662-453X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/202305
identifier_str_mv Asatryan, Aram; Calandria, Jorgelina M.; Kautzmann, Marie-Audrey; Jun, Bokkyoo; Gordon, William C.; et al.; New Retinal Pigment Epithelial Cell Model to Unravel Neuroprotection Sensors of Neurodegeneration in Retinal Disease; Frontiers Media; Frontiers in Neuroscience; 16; 926629; 30-6-2022; 1-18
1662-453X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fnins.2022.926629/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fnins.2022.926629
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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