Druse-Induced Morphology Evolution in Retinal Pigment Epithelium

Autores
Mazzitello, Karina Irma; Zhang, Qing; Chrenek, M. A.; Family, F.; Grossniklaus, H. E.; Nickerson, J. M.; Jiang, Y.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The retinal pigment epithelium (RPE) is a key site of pathogenesis for many retina diseases. The formation of drusen in the retina is characteristic of retinal degeneration. We investigate morphological changes in the RPE in the presence of soft drusen using an integrated experimental and modeling approach. We collect RPE flat mount images from donated human eyes and develop 1) statistical tools to quantify the images and 2) a cell-based model to simulate the morphology evolution. We compare three different mechanisms of RPE repair evolution, cell apoptosis, cell fusion, and expansion, and Simulations of our RPE morphogenesis model quantitatively reproduce deformations of human RPE morphology due to drusen, suggesting that a purse-string mechanism is sufficient to explain how RPE heals cell loss caused by drusen-damage. We found that drusen beneath tissue promote cell death in a number that far exceeds the cell numbers covering the drusen. Tissue deformations are studied using area distributions, Voronoi domains and a texture tensor.
Fil: Mazzitello, Karina Irma. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Zhang, Qing. University of Emory; Estados Unidos
Fil: Chrenek, M. A.. University of Emory; Estados Unidos
Fil: Family, F.. University of Emory; Estados Unidos
Fil: Grossniklaus, H. E.. University of Emory; Estados Unidos
Fil: Nickerson, J. M.. University of Emory; Estados Unidos
Fil: Jiang, Y.. Georgia State University; Estados Unidos
Materia
Macular Degeneration
Drusen
Retinal pigment Epithelium
Morphology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92249
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Druse-Induced Morphology Evolution in Retinal Pigment EpitheliumMazzitello, Karina IrmaZhang, QingChrenek, M. A.Family, F.Grossniklaus, H. E.Nickerson, J. M.Jiang, Y.Macular DegenerationDrusenRetinal pigment EpitheliumMorphologyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The retinal pigment epithelium (RPE) is a key site of pathogenesis for many retina diseases. The formation of drusen in the retina is characteristic of retinal degeneration. We investigate morphological changes in the RPE in the presence of soft drusen using an integrated experimental and modeling approach. We collect RPE flat mount images from donated human eyes and develop 1) statistical tools to quantify the images and 2) a cell-based model to simulate the morphology evolution. We compare three different mechanisms of RPE repair evolution, cell apoptosis, cell fusion, and expansion, and Simulations of our RPE morphogenesis model quantitatively reproduce deformations of human RPE morphology due to drusen, suggesting that a purse-string mechanism is sufficient to explain how RPE heals cell loss caused by drusen-damage. We found that drusen beneath tissue promote cell death in a number that far exceeds the cell numbers covering the drusen. Tissue deformations are studied using area distributions, Voronoi domains and a texture tensor.Fil: Mazzitello, Karina Irma. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Zhang, Qing. University of Emory; Estados UnidosFil: Chrenek, M. A.. University of Emory; Estados UnidosFil: Family, F.. University of Emory; Estados UnidosFil: Grossniklaus, H. E.. University of Emory; Estados UnidosFil: Nickerson, J. M.. University of Emory; Estados UnidosFil: Jiang, Y.. Georgia State University; Estados UnidosCornell University2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92249Mazzitello, Karina Irma; Zhang, Qing; Chrenek, M. A.; Family, F.; Grossniklaus, H. E.; et al.; Druse-Induced Morphology Evolution in Retinal Pigment Epithelium; Cornell University; arXiv; 9-20162331-8422CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://arxiv.org/abs/1609.04496info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:26Zoai:ri.conicet.gov.ar:11336/92249instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:26.605CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Druse-Induced Morphology Evolution in Retinal Pigment Epithelium
title Druse-Induced Morphology Evolution in Retinal Pigment Epithelium
spellingShingle Druse-Induced Morphology Evolution in Retinal Pigment Epithelium
Mazzitello, Karina Irma
Macular Degeneration
Drusen
Retinal pigment Epithelium
Morphology
title_short Druse-Induced Morphology Evolution in Retinal Pigment Epithelium
title_full Druse-Induced Morphology Evolution in Retinal Pigment Epithelium
title_fullStr Druse-Induced Morphology Evolution in Retinal Pigment Epithelium
title_full_unstemmed Druse-Induced Morphology Evolution in Retinal Pigment Epithelium
title_sort Druse-Induced Morphology Evolution in Retinal Pigment Epithelium
dc.creator.none.fl_str_mv Mazzitello, Karina Irma
Zhang, Qing
Chrenek, M. A.
Family, F.
Grossniklaus, H. E.
Nickerson, J. M.
Jiang, Y.
author Mazzitello, Karina Irma
author_facet Mazzitello, Karina Irma
Zhang, Qing
Chrenek, M. A.
Family, F.
Grossniklaus, H. E.
Nickerson, J. M.
Jiang, Y.
author_role author
author2 Zhang, Qing
Chrenek, M. A.
Family, F.
Grossniklaus, H. E.
Nickerson, J. M.
Jiang, Y.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Macular Degeneration
Drusen
Retinal pigment Epithelium
Morphology
topic Macular Degeneration
Drusen
Retinal pigment Epithelium
Morphology
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The retinal pigment epithelium (RPE) is a key site of pathogenesis for many retina diseases. The formation of drusen in the retina is characteristic of retinal degeneration. We investigate morphological changes in the RPE in the presence of soft drusen using an integrated experimental and modeling approach. We collect RPE flat mount images from donated human eyes and develop 1) statistical tools to quantify the images and 2) a cell-based model to simulate the morphology evolution. We compare three different mechanisms of RPE repair evolution, cell apoptosis, cell fusion, and expansion, and Simulations of our RPE morphogenesis model quantitatively reproduce deformations of human RPE morphology due to drusen, suggesting that a purse-string mechanism is sufficient to explain how RPE heals cell loss caused by drusen-damage. We found that drusen beneath tissue promote cell death in a number that far exceeds the cell numbers covering the drusen. Tissue deformations are studied using area distributions, Voronoi domains and a texture tensor.
Fil: Mazzitello, Karina Irma. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Zhang, Qing. University of Emory; Estados Unidos
Fil: Chrenek, M. A.. University of Emory; Estados Unidos
Fil: Family, F.. University of Emory; Estados Unidos
Fil: Grossniklaus, H. E.. University of Emory; Estados Unidos
Fil: Nickerson, J. M.. University of Emory; Estados Unidos
Fil: Jiang, Y.. Georgia State University; Estados Unidos
description The retinal pigment epithelium (RPE) is a key site of pathogenesis for many retina diseases. The formation of drusen in the retina is characteristic of retinal degeneration. We investigate morphological changes in the RPE in the presence of soft drusen using an integrated experimental and modeling approach. We collect RPE flat mount images from donated human eyes and develop 1) statistical tools to quantify the images and 2) a cell-based model to simulate the morphology evolution. We compare three different mechanisms of RPE repair evolution, cell apoptosis, cell fusion, and expansion, and Simulations of our RPE morphogenesis model quantitatively reproduce deformations of human RPE morphology due to drusen, suggesting that a purse-string mechanism is sufficient to explain how RPE heals cell loss caused by drusen-damage. We found that drusen beneath tissue promote cell death in a number that far exceeds the cell numbers covering the drusen. Tissue deformations are studied using area distributions, Voronoi domains and a texture tensor.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92249
Mazzitello, Karina Irma; Zhang, Qing; Chrenek, M. A.; Family, F.; Grossniklaus, H. E.; et al.; Druse-Induced Morphology Evolution in Retinal Pigment Epithelium; Cornell University; arXiv; 9-2016
2331-8422
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92249
identifier_str_mv Mazzitello, Karina Irma; Zhang, Qing; Chrenek, M. A.; Family, F.; Grossniklaus, H. E.; et al.; Druse-Induced Morphology Evolution in Retinal Pigment Epithelium; Cornell University; arXiv; 9-2016
2331-8422
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://arxiv.org/abs/1609.04496
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cornell University
publisher.none.fl_str_mv Cornell University
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268794854047744
score 13.13397

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