RPE cell and sheet properties in normal and diseased eyes

Autores
Rashid, Alia; Bhatia, Shagun K.; Mazzitello, Karina Irma; Chrenek, Micah A.; Zhang, Qing; Boatright, Jeffrey H.; Grossniklaus, Hans E.; Jiang, Yi; Nickerson, John M.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Previous studies of human retinal pigment epithelium (RPE) morphology found spatial differences in density: a high density of cells in the macula, decreasing peripherally. Because the RPE sheet is not perfectly regular, we anticipate that there will be differences between conditions and when and where damage is most likely to begin. The purpose of this study is to establish relationships among RPE morphometrics in age, cell location, and disease of normal human and AMD eyes that highlight irregularities reflecting damage. Cadaveric eyes from 11 normal and 3 age-related macular degeneration (AMD) human donors ranging from 29 to 82 years of age were used. Borders of RPE cells were identified with phalloidin. RPE segmentation and analysis were conducted with CellProfiler. Exploration of spatial point patterns was conducted using the “spatstat” package of R. In the normal human eye, with increasing age, cell size increased, and cells lost their regular hexagonal shape. Cell density was higher in the macula versus periphery. AMD resulted in greater variability in size and shape of the RPE cell. Spatial point analysis revealed an ordered distribution of cells in normal and high spatial disorder in AMD eyes. Morphometrics of the RPE cell readily discriminate among young vs. old and normal vs. diseased in the human eye. The normal RPE sheet is organized in a regular array of cells, but AMD exhibited strong spatial irregularity. These findings reflect on the robust recovery of the RPE sheet after wounding and the circumstances under which it cannot recover.
Fil: Rashid, Alia. Emory University; Estados Unidos
Fil: Bhatia, Shagun K.. Emory University; Estados Unidos
Fil: Mazzitello, Karina Irma. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Chrenek, Micah A.. Emory University; Estados Unidos
Fil: Zhang, Qing. Emory University; Estados Unidos
Fil: Boatright, Jeffrey H.. Emory University; Estados Unidos
Fil: Grossniklaus, Hans E.. Emory University; Estados Unidos
Fil: Jiang, Yi. Georgia State University; Estados Unidos
Fil: Nickerson, John M.. Emory Eye Center; Estados Unidos
Materia
Age Related Macular Degeneration (Amd)
Cadaveric Eyes
Cellprofiler
En Face
Flatmount
Macula
Nearest Neighbor Distance
Periphery
Retinal Pigmented Epithelium (Rpe)
Spatial Point Patterns
Spatstat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50712
Acceder
id CONICETDig_b1ed53507125af3ef2ad7ec96cfa9277
oai_identifier_str oai:ri.conicet.gov.ar:11336/50712
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling RPE cell and sheet properties in normal and diseased eyesRashid, AliaBhatia, Shagun K.Mazzitello, Karina IrmaChrenek, Micah A.Zhang, QingBoatright, Jeffrey H.Grossniklaus, Hans E.Jiang, YiNickerson, John M.Age Related Macular Degeneration (Amd)Cadaveric EyesCellprofilerEn FaceFlatmountMaculaNearest Neighbor DistancePeripheryRetinal Pigmented Epithelium (Rpe)Spatial Point PatternsSpatstathttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Previous studies of human retinal pigment epithelium (RPE) morphology found spatial differences in density: a high density of cells in the macula, decreasing peripherally. Because the RPE sheet is not perfectly regular, we anticipate that there will be differences between conditions and when and where damage is most likely to begin. The purpose of this study is to establish relationships among RPE morphometrics in age, cell location, and disease of normal human and AMD eyes that highlight irregularities reflecting damage. Cadaveric eyes from 11 normal and 3 age-related macular degeneration (AMD) human donors ranging from 29 to 82 years of age were used. Borders of RPE cells were identified with phalloidin. RPE segmentation and analysis were conducted with CellProfiler. Exploration of spatial point patterns was conducted using the “spatstat” package of R. In the normal human eye, with increasing age, cell size increased, and cells lost their regular hexagonal shape. Cell density was higher in the macula versus periphery. AMD resulted in greater variability in size and shape of the RPE cell. Spatial point analysis revealed an ordered distribution of cells in normal and high spatial disorder in AMD eyes. Morphometrics of the RPE cell readily discriminate among young vs. old and normal vs. diseased in the human eye. The normal RPE sheet is organized in a regular array of cells, but AMD exhibited strong spatial irregularity. These findings reflect on the robust recovery of the RPE sheet after wounding and the circumstances under which it cannot recover.Fil: Rashid, Alia. Emory University; Estados UnidosFil: Bhatia, Shagun K.. Emory University; Estados UnidosFil: Mazzitello, Karina Irma. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Chrenek, Micah A.. Emory University; Estados UnidosFil: Zhang, Qing. Emory University; Estados UnidosFil: Boatright, Jeffrey H.. Emory University; Estados UnidosFil: Grossniklaus, Hans E.. Emory University; Estados UnidosFil: Jiang, Yi. Georgia State University; Estados UnidosFil: Nickerson, John M.. Emory Eye Center; Estados UnidosSpringer Verlag Berlín2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50712Rashid, Alia; Bhatia, Shagun K.; Mazzitello, Karina Irma; Chrenek, Micah A.; Zhang, Qing; et al.; RPE cell and sheet properties in normal and diseased eyes; Springer Verlag Berlín; Advances in Experimental Medicine and Biology; 854; 10-2016; 757-7630065-2598CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/978-3-319-17121-0_101info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/chapter/10.1007%2F978-3-319-17121-0_101info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/23692342/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:28:06Zoai:ri.conicet.gov.ar:11336/50712instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:28:07.013CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv RPE cell and sheet properties in normal and diseased eyes
title RPE cell and sheet properties in normal and diseased eyes
spellingShingle RPE cell and sheet properties in normal and diseased eyes
Rashid, Alia
Age Related Macular Degeneration (Amd)
Cadaveric Eyes
Cellprofiler
En Face
Flatmount
Macula
Nearest Neighbor Distance
Periphery
Retinal Pigmented Epithelium (Rpe)
Spatial Point Patterns
Spatstat
title_short RPE cell and sheet properties in normal and diseased eyes
title_full RPE cell and sheet properties in normal and diseased eyes
title_fullStr RPE cell and sheet properties in normal and diseased eyes
title_full_unstemmed RPE cell and sheet properties in normal and diseased eyes
title_sort RPE cell and sheet properties in normal and diseased eyes
dc.creator.none.fl_str_mv Rashid, Alia
Bhatia, Shagun K.
Mazzitello, Karina Irma
Chrenek, Micah A.
Zhang, Qing
Boatright, Jeffrey H.
Grossniklaus, Hans E.
Jiang, Yi
Nickerson, John M.
author Rashid, Alia
author_facet Rashid, Alia
Bhatia, Shagun K.
Mazzitello, Karina Irma
Chrenek, Micah A.
Zhang, Qing
Boatright, Jeffrey H.
Grossniklaus, Hans E.
Jiang, Yi
Nickerson, John M.
author_role author
author2 Bhatia, Shagun K.
Mazzitello, Karina Irma
Chrenek, Micah A.
Zhang, Qing
Boatright, Jeffrey H.
Grossniklaus, Hans E.
Jiang, Yi
Nickerson, John M.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Age Related Macular Degeneration (Amd)
Cadaveric Eyes
Cellprofiler
En Face
Flatmount
Macula
Nearest Neighbor Distance
Periphery
Retinal Pigmented Epithelium (Rpe)
Spatial Point Patterns
Spatstat
topic Age Related Macular Degeneration (Amd)
Cadaveric Eyes
Cellprofiler
En Face
Flatmount
Macula
Nearest Neighbor Distance
Periphery
Retinal Pigmented Epithelium (Rpe)
Spatial Point Patterns
Spatstat
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Previous studies of human retinal pigment epithelium (RPE) morphology found spatial differences in density: a high density of cells in the macula, decreasing peripherally. Because the RPE sheet is not perfectly regular, we anticipate that there will be differences between conditions and when and where damage is most likely to begin. The purpose of this study is to establish relationships among RPE morphometrics in age, cell location, and disease of normal human and AMD eyes that highlight irregularities reflecting damage. Cadaveric eyes from 11 normal and 3 age-related macular degeneration (AMD) human donors ranging from 29 to 82 years of age were used. Borders of RPE cells were identified with phalloidin. RPE segmentation and analysis were conducted with CellProfiler. Exploration of spatial point patterns was conducted using the “spatstat” package of R. In the normal human eye, with increasing age, cell size increased, and cells lost their regular hexagonal shape. Cell density was higher in the macula versus periphery. AMD resulted in greater variability in size and shape of the RPE cell. Spatial point analysis revealed an ordered distribution of cells in normal and high spatial disorder in AMD eyes. Morphometrics of the RPE cell readily discriminate among young vs. old and normal vs. diseased in the human eye. The normal RPE sheet is organized in a regular array of cells, but AMD exhibited strong spatial irregularity. These findings reflect on the robust recovery of the RPE sheet after wounding and the circumstances under which it cannot recover.
Fil: Rashid, Alia. Emory University; Estados Unidos
Fil: Bhatia, Shagun K.. Emory University; Estados Unidos
Fil: Mazzitello, Karina Irma. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Chrenek, Micah A.. Emory University; Estados Unidos
Fil: Zhang, Qing. Emory University; Estados Unidos
Fil: Boatright, Jeffrey H.. Emory University; Estados Unidos
Fil: Grossniklaus, Hans E.. Emory University; Estados Unidos
Fil: Jiang, Yi. Georgia State University; Estados Unidos
Fil: Nickerson, John M.. Emory Eye Center; Estados Unidos
description Previous studies of human retinal pigment epithelium (RPE) morphology found spatial differences in density: a high density of cells in the macula, decreasing peripherally. Because the RPE sheet is not perfectly regular, we anticipate that there will be differences between conditions and when and where damage is most likely to begin. The purpose of this study is to establish relationships among RPE morphometrics in age, cell location, and disease of normal human and AMD eyes that highlight irregularities reflecting damage. Cadaveric eyes from 11 normal and 3 age-related macular degeneration (AMD) human donors ranging from 29 to 82 years of age were used. Borders of RPE cells were identified with phalloidin. RPE segmentation and analysis were conducted with CellProfiler. Exploration of spatial point patterns was conducted using the “spatstat” package of R. In the normal human eye, with increasing age, cell size increased, and cells lost their regular hexagonal shape. Cell density was higher in the macula versus periphery. AMD resulted in greater variability in size and shape of the RPE cell. Spatial point analysis revealed an ordered distribution of cells in normal and high spatial disorder in AMD eyes. Morphometrics of the RPE cell readily discriminate among young vs. old and normal vs. diseased in the human eye. The normal RPE sheet is organized in a regular array of cells, but AMD exhibited strong spatial irregularity. These findings reflect on the robust recovery of the RPE sheet after wounding and the circumstances under which it cannot recover.
publishDate 2016
dc.date.none.fl_str_mv 2016-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50712
Rashid, Alia; Bhatia, Shagun K.; Mazzitello, Karina Irma; Chrenek, Micah A.; Zhang, Qing; et al.; RPE cell and sheet properties in normal and diseased eyes; Springer Verlag Berlín; Advances in Experimental Medicine and Biology; 854; 10-2016; 757-763
0065-2598
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50712
identifier_str_mv Rashid, Alia; Bhatia, Shagun K.; Mazzitello, Karina Irma; Chrenek, Micah A.; Zhang, Qing; et al.; RPE cell and sheet properties in normal and diseased eyes; Springer Verlag Berlín; Advances in Experimental Medicine and Biology; 854; 10-2016; 757-763
0065-2598
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/978-3-319-17121-0_101
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/chapter/10.1007%2F978-3-319-17121-0_101
info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/23692342/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Verlag Berlín
publisher.none.fl_str_mv Springer Verlag Berlín
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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