Nitric oxide synthase 2 (NOS2) expression in histologically normal margins of oral squamous cell carcinoma

Autores
Morelatto, Rosana Andrea; Itoiz, María Elina; Guiñazu Alaniz, Natalia Lorena; Piccini, Daniel; Gea, Susana; Lopez de Blanc, Silvia Adriana
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The activity of Nitric Oxide Synthase 2 (NOS2) was found in oral squamous cell carcinomas (OSCC) but not in normal mucosa. Molecular changes associated to early carcinogenesis have been found in mucosa near carcinomas, which is considered a model to study field cancerization. The aim of the present study is to analyze NOS2 expression at the histologically normal margins of OSCC. Study Design: Eleven biopsy specimens of OSCC containing histologically normal margins (HNM) were analyzed. Ten biopsies of normal oral mucosa were used as controls. The activity of NOS2 was determined by immunohistochemistry. Salivary nitrate and nitrite as well as tobacco and alcohol consumption were also analyzed. The Chi-squared test was applied. Results: Six out of the eleven HNM from carcinoma samples showed positive NOS2 activity whereas all the control group samples yielded negative (p=0.005). No statistically significant association between enzyme expression and tobacco and/or alcohol consumption and salivary nitrate and nitrite was found. Conclusion: NOS2 expression would be an additional evidence of alterations that may occur in a state of field cancerization before the appearance of potentially malignant morphological changes.
Fil: Morelatto, Rosana Andrea. Universidad Nacional de Córdoba; Argentina
Fil: Itoiz, María Elina. Universidad de Buenos Aires; Argentina
Fil: Guiñazu Alaniz, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas; Argentina
Fil: Piccini, Daniel. Universidad Nacional de Córdoba; Argentina
Fil: Gea, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Lopez de Blanc, Silvia Adriana. Universidad Nacional de Córdoba; Argentina
Materia
Field Cancerization
Oral Squamous Cell Carcinoma
Nitric Oxide Synthase 2 (Nos2)
Malignity Markers
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40581
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/40581
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nitric oxide synthase 2 (NOS2) expression in histologically normal margins of oral squamous cell carcinomaMorelatto, Rosana AndreaItoiz, María ElinaGuiñazu Alaniz, Natalia LorenaPiccini, DanielGea, SusanaLopez de Blanc, Silvia AdrianaField CancerizationOral Squamous Cell CarcinomaNitric Oxide Synthase 2 (Nos2)Malignity Markershttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The activity of Nitric Oxide Synthase 2 (NOS2) was found in oral squamous cell carcinomas (OSCC) but not in normal mucosa. Molecular changes associated to early carcinogenesis have been found in mucosa near carcinomas, which is considered a model to study field cancerization. The aim of the present study is to analyze NOS2 expression at the histologically normal margins of OSCC. Study Design: Eleven biopsy specimens of OSCC containing histologically normal margins (HNM) were analyzed. Ten biopsies of normal oral mucosa were used as controls. The activity of NOS2 was determined by immunohistochemistry. Salivary nitrate and nitrite as well as tobacco and alcohol consumption were also analyzed. The Chi-squared test was applied. Results: Six out of the eleven HNM from carcinoma samples showed positive NOS2 activity whereas all the control group samples yielded negative (p=0.005). No statistically significant association between enzyme expression and tobacco and/or alcohol consumption and salivary nitrate and nitrite was found. Conclusion: NOS2 expression would be an additional evidence of alterations that may occur in a state of field cancerization before the appearance of potentially malignant morphological changes.Fil: Morelatto, Rosana Andrea. Universidad Nacional de Córdoba; ArgentinaFil: Itoiz, María Elina. Universidad de Buenos Aires; ArgentinaFil: Guiñazu Alaniz, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas; ArgentinaFil: Piccini, Daniel. Universidad Nacional de Córdoba; ArgentinaFil: Gea, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Lopez de Blanc, Silvia Adriana. Universidad Nacional de Córdoba; ArgentinaMedicina Oral S L2014-05-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40581Morelatto, Rosana Andrea; Itoiz, María Elina; Guiñazu Alaniz, Natalia Lorena; Piccini, Daniel; Gea, Susana; et al.; Nitric oxide synthase 2 (NOS2) expression in histologically normal margins of oral squamous cell carcinoma; Medicina Oral S L; Medicina Oral Patologia Oral y Cirugia Bucal; 19; 3; 7-5-2014; 242-2471698-4447CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4317/medoral.19351info:eu-repo/semantics/altIdentifier/url/http://www.medicinaoral.com/pubmed/medoralv19_i3_p242.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:20:19Zoai:ri.conicet.gov.ar:11336/40581instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:20:20.292CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nitric oxide synthase 2 (NOS2) expression in histologically normal margins of oral squamous cell carcinoma
title Nitric oxide synthase 2 (NOS2) expression in histologically normal margins of oral squamous cell carcinoma
spellingShingle Nitric oxide synthase 2 (NOS2) expression in histologically normal margins of oral squamous cell carcinoma
Morelatto, Rosana Andrea
Field Cancerization
Oral Squamous Cell Carcinoma
Nitric Oxide Synthase 2 (Nos2)
Malignity Markers
title_short Nitric oxide synthase 2 (NOS2) expression in histologically normal margins of oral squamous cell carcinoma
title_full Nitric oxide synthase 2 (NOS2) expression in histologically normal margins of oral squamous cell carcinoma
title_fullStr Nitric oxide synthase 2 (NOS2) expression in histologically normal margins of oral squamous cell carcinoma
title_full_unstemmed Nitric oxide synthase 2 (NOS2) expression in histologically normal margins of oral squamous cell carcinoma
title_sort Nitric oxide synthase 2 (NOS2) expression in histologically normal margins of oral squamous cell carcinoma
dc.creator.none.fl_str_mv Morelatto, Rosana Andrea
Itoiz, María Elina
Guiñazu Alaniz, Natalia Lorena
Piccini, Daniel
Gea, Susana
Lopez de Blanc, Silvia Adriana
author Morelatto, Rosana Andrea
author_facet Morelatto, Rosana Andrea
Itoiz, María Elina
Guiñazu Alaniz, Natalia Lorena
Piccini, Daniel
Gea, Susana
Lopez de Blanc, Silvia Adriana
author_role author
author2 Itoiz, María Elina
Guiñazu Alaniz, Natalia Lorena
Piccini, Daniel
Gea, Susana
Lopez de Blanc, Silvia Adriana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Field Cancerization
Oral Squamous Cell Carcinoma
Nitric Oxide Synthase 2 (Nos2)
Malignity Markers
topic Field Cancerization
Oral Squamous Cell Carcinoma
Nitric Oxide Synthase 2 (Nos2)
Malignity Markers
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The activity of Nitric Oxide Synthase 2 (NOS2) was found in oral squamous cell carcinomas (OSCC) but not in normal mucosa. Molecular changes associated to early carcinogenesis have been found in mucosa near carcinomas, which is considered a model to study field cancerization. The aim of the present study is to analyze NOS2 expression at the histologically normal margins of OSCC. Study Design: Eleven biopsy specimens of OSCC containing histologically normal margins (HNM) were analyzed. Ten biopsies of normal oral mucosa were used as controls. The activity of NOS2 was determined by immunohistochemistry. Salivary nitrate and nitrite as well as tobacco and alcohol consumption were also analyzed. The Chi-squared test was applied. Results: Six out of the eleven HNM from carcinoma samples showed positive NOS2 activity whereas all the control group samples yielded negative (p=0.005). No statistically significant association between enzyme expression and tobacco and/or alcohol consumption and salivary nitrate and nitrite was found. Conclusion: NOS2 expression would be an additional evidence of alterations that may occur in a state of field cancerization before the appearance of potentially malignant morphological changes.
Fil: Morelatto, Rosana Andrea. Universidad Nacional de Córdoba; Argentina
Fil: Itoiz, María Elina. Universidad de Buenos Aires; Argentina
Fil: Guiñazu Alaniz, Natalia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energias Alternativas; Argentina
Fil: Piccini, Daniel. Universidad Nacional de Córdoba; Argentina
Fil: Gea, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Lopez de Blanc, Silvia Adriana. Universidad Nacional de Córdoba; Argentina
description The activity of Nitric Oxide Synthase 2 (NOS2) was found in oral squamous cell carcinomas (OSCC) but not in normal mucosa. Molecular changes associated to early carcinogenesis have been found in mucosa near carcinomas, which is considered a model to study field cancerization. The aim of the present study is to analyze NOS2 expression at the histologically normal margins of OSCC. Study Design: Eleven biopsy specimens of OSCC containing histologically normal margins (HNM) were analyzed. Ten biopsies of normal oral mucosa were used as controls. The activity of NOS2 was determined by immunohistochemistry. Salivary nitrate and nitrite as well as tobacco and alcohol consumption were also analyzed. The Chi-squared test was applied. Results: Six out of the eleven HNM from carcinoma samples showed positive NOS2 activity whereas all the control group samples yielded negative (p=0.005). No statistically significant association between enzyme expression and tobacco and/or alcohol consumption and salivary nitrate and nitrite was found. Conclusion: NOS2 expression would be an additional evidence of alterations that may occur in a state of field cancerization before the appearance of potentially malignant morphological changes.
publishDate 2014
dc.date.none.fl_str_mv 2014-05-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40581
Morelatto, Rosana Andrea; Itoiz, María Elina; Guiñazu Alaniz, Natalia Lorena; Piccini, Daniel; Gea, Susana; et al.; Nitric oxide synthase 2 (NOS2) expression in histologically normal margins of oral squamous cell carcinoma; Medicina Oral S L; Medicina Oral Patologia Oral y Cirugia Bucal; 19; 3; 7-5-2014; 242-247
1698-4447
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40581
identifier_str_mv Morelatto, Rosana Andrea; Itoiz, María Elina; Guiñazu Alaniz, Natalia Lorena; Piccini, Daniel; Gea, Susana; et al.; Nitric oxide synthase 2 (NOS2) expression in histologically normal margins of oral squamous cell carcinoma; Medicina Oral S L; Medicina Oral Patologia Oral y Cirugia Bucal; 19; 3; 7-5-2014; 242-247
1698-4447
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4317/medoral.19351
info:eu-repo/semantics/altIdentifier/url/http://www.medicinaoral.com/pubmed/medoralv19_i3_p242.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Medicina Oral S L
publisher.none.fl_str_mv Medicina Oral S L
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781693083516928
score 12.982451

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