Inflammasome activation is critical to the protective immune response during chemically induced squamous cell carcinoma

Autores
Gasparoto, Thais Helena; Ervolino de Oliveira, Carine; Thomazini de Freitas, Luisa; Ramos Pinheiro, Claudia; Issa Hori, Juliana; Pompermaier Garlet, Gustavo; Cavassani, Karen Angélica; Schillaci, Roxana; Santana Da Silva, Joao; Simmões Zamboni, Darío; Campanelli, Ana Paula
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chronic inflammation affects most stages of tumorigenesis, including initiation, promotion, malignant differentiation, invasion and metastasis. Inflammasomes have been described as involved with persistent inflammation and are known to exert both pro and antitumour effects. We evaluated the influence of apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and caspase (CASP)-1 in the antitumor immune response using a multistage model of squamous cell carcinoma (SCC) development. Absence of ASC and CASP-1 resulted in an earlier incidence and increased number of papilloma. Loss of inflammassome function in mice resulted in decreased presence of natural killer (NK), dendritic (DC), CD4+, CD8+ and CD45RB+ T cells in the tumor lesions as well as in lymph nodes (LN) compared with WT mice. Increased percentage of CD4+CD25+Foxp3+ T cells was associated with association with inflammasome loss of function. Moreover, significant differences were also found with neutrophils and macrophage infiltrating the lesions. Myeloperoxidase (MPO), but not elastase (ELA), activity oscillated among the groups during the SCC development. Levels of proinflammatory cytokines IL-1β, IL-18, Tumor Necrosis Factor (TNF)-α and Interferon (IFN)-γ were decreased in the tumor microenvironment in the absence of inflammasome proteins. These observations suggest a link between inflammasome function and SCC tumorigenesis, indicating an important role for inflammasome activation in the control of SCC development.
Fil: Gasparoto, Thais Helena. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; Brasil
Fil: Ervolino de Oliveira, Carine. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; Brasil
Fil: Thomazini de Freitas, Luisa. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; Brasil
Fil: Ramos Pinheiro, Claudia. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; Brasil
Fil: Issa Hori, Juliana. University of São Paulo. Faculdade de Medicina de Ribeirão Preto; Brasil
Fil: Pompermaier Garlet, Gustavo. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; Brasil
Fil: Cavassani, Karen Angélica. University Of Michigan; Estados Unidos
Fil: Schillaci, Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Santana Da Silva, Joao. University of São Paulo. Faculdade de Medicina de Ribeirão Preto; Brasil
Fil: Simmões Zamboni, Darío. University of São Paulo. Faculdade de Medicina de Ribeirão Preto; Brasil
Fil: Campanelli, Ana Paula. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; Brasil
Materia
INFLAMOSOMES
SQUAMOUS CELL CARCINOMA
PAPILLOMA
SKIN NEOPLASMS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6438
Acceder
id CONICETDig_58ba202b368fbfbaba1f86e26504ac9d
oai_identifier_str oai:ri.conicet.gov.ar:11336/6438
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inflammasome activation is critical to the protective immune response during chemically induced squamous cell carcinomaGasparoto, Thais HelenaErvolino de Oliveira, CarineThomazini de Freitas, LuisaRamos Pinheiro, ClaudiaIssa Hori, JulianaPompermaier Garlet, GustavoCavassani, Karen AngélicaSchillaci, RoxanaSantana Da Silva, JoaoSimmões Zamboni, DaríoCampanelli, Ana PaulaINFLAMOSOMESSQUAMOUS CELL CARCINOMAPAPILLOMASKIN NEOPLASMShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Chronic inflammation affects most stages of tumorigenesis, including initiation, promotion, malignant differentiation, invasion and metastasis. Inflammasomes have been described as involved with persistent inflammation and are known to exert both pro and antitumour effects. We evaluated the influence of apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and caspase (CASP)-1 in the antitumor immune response using a multistage model of squamous cell carcinoma (SCC) development. Absence of ASC and CASP-1 resulted in an earlier incidence and increased number of papilloma. Loss of inflammassome function in mice resulted in decreased presence of natural killer (NK), dendritic (DC), CD4+, CD8+ and CD45RB+ T cells in the tumor lesions as well as in lymph nodes (LN) compared with WT mice. Increased percentage of CD4+CD25+Foxp3+ T cells was associated with association with inflammasome loss of function. Moreover, significant differences were also found with neutrophils and macrophage infiltrating the lesions. Myeloperoxidase (MPO), but not elastase (ELA), activity oscillated among the groups during the SCC development. Levels of proinflammatory cytokines IL-1β, IL-18, Tumor Necrosis Factor (TNF)-α and Interferon (IFN)-γ were decreased in the tumor microenvironment in the absence of inflammasome proteins. These observations suggest a link between inflammasome function and SCC tumorigenesis, indicating an important role for inflammasome activation in the control of SCC development.Fil: Gasparoto, Thais Helena. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; BrasilFil: Ervolino de Oliveira, Carine. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; BrasilFil: Thomazini de Freitas, Luisa. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; BrasilFil: Ramos Pinheiro, Claudia. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; BrasilFil: Issa Hori, Juliana. University of São Paulo. Faculdade de Medicina de Ribeirão Preto; BrasilFil: Pompermaier Garlet, Gustavo. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; BrasilFil: Cavassani, Karen Angélica. University Of Michigan; Estados UnidosFil: Schillaci, Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Santana Da Silva, Joao. University of São Paulo. Faculdade de Medicina de Ribeirão Preto; BrasilFil: Simmões Zamboni, Darío. University of São Paulo. Faculdade de Medicina de Ribeirão Preto; BrasilFil: Campanelli, Ana Paula. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; BrasilPublic Library of Science2014-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6438Gasparoto, Thais Helena; Ervolino de Oliveira, Carine; Thomazini de Freitas, Luisa; Ramos Pinheiro, Claudia; Issa Hori, Juliana; et al.; Inflammasome activation is critical to the protective immune response during chemically induced squamous cell carcinoma; Public Library of Science; Plos One; 9; 9; 30-9-2014; e107170-e1071701932-62031932-6203enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182037/info:eu-repo/semantics/altIdentifier/pmid/25268644info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0107170info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0107170info:eu-repo/semantics/altIdentifier/pmid/PMC4182037info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:11:44Zoai:ri.conicet.gov.ar:11336/6438instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:11:44.96CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inflammasome activation is critical to the protective immune response during chemically induced squamous cell carcinoma
title Inflammasome activation is critical to the protective immune response during chemically induced squamous cell carcinoma
spellingShingle Inflammasome activation is critical to the protective immune response during chemically induced squamous cell carcinoma
Gasparoto, Thais Helena
INFLAMOSOMES
SQUAMOUS CELL CARCINOMA
PAPILLOMA
SKIN NEOPLASMS
title_short Inflammasome activation is critical to the protective immune response during chemically induced squamous cell carcinoma
title_full Inflammasome activation is critical to the protective immune response during chemically induced squamous cell carcinoma
title_fullStr Inflammasome activation is critical to the protective immune response during chemically induced squamous cell carcinoma
title_full_unstemmed Inflammasome activation is critical to the protective immune response during chemically induced squamous cell carcinoma
title_sort Inflammasome activation is critical to the protective immune response during chemically induced squamous cell carcinoma
dc.creator.none.fl_str_mv Gasparoto, Thais Helena
Ervolino de Oliveira, Carine
Thomazini de Freitas, Luisa
Ramos Pinheiro, Claudia
Issa Hori, Juliana
Pompermaier Garlet, Gustavo
Cavassani, Karen Angélica
Schillaci, Roxana
Santana Da Silva, Joao
Simmões Zamboni, Darío
Campanelli, Ana Paula
author Gasparoto, Thais Helena
author_facet Gasparoto, Thais Helena
Ervolino de Oliveira, Carine
Thomazini de Freitas, Luisa
Ramos Pinheiro, Claudia
Issa Hori, Juliana
Pompermaier Garlet, Gustavo
Cavassani, Karen Angélica
Schillaci, Roxana
Santana Da Silva, Joao
Simmões Zamboni, Darío
Campanelli, Ana Paula
author_role author
author2 Ervolino de Oliveira, Carine
Thomazini de Freitas, Luisa
Ramos Pinheiro, Claudia
Issa Hori, Juliana
Pompermaier Garlet, Gustavo
Cavassani, Karen Angélica
Schillaci, Roxana
Santana Da Silva, Joao
Simmões Zamboni, Darío
Campanelli, Ana Paula
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv INFLAMOSOMES
SQUAMOUS CELL CARCINOMA
PAPILLOMA
SKIN NEOPLASMS
topic INFLAMOSOMES
SQUAMOUS CELL CARCINOMA
PAPILLOMA
SKIN NEOPLASMS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Chronic inflammation affects most stages of tumorigenesis, including initiation, promotion, malignant differentiation, invasion and metastasis. Inflammasomes have been described as involved with persistent inflammation and are known to exert both pro and antitumour effects. We evaluated the influence of apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and caspase (CASP)-1 in the antitumor immune response using a multistage model of squamous cell carcinoma (SCC) development. Absence of ASC and CASP-1 resulted in an earlier incidence and increased number of papilloma. Loss of inflammassome function in mice resulted in decreased presence of natural killer (NK), dendritic (DC), CD4+, CD8+ and CD45RB+ T cells in the tumor lesions as well as in lymph nodes (LN) compared with WT mice. Increased percentage of CD4+CD25+Foxp3+ T cells was associated with association with inflammasome loss of function. Moreover, significant differences were also found with neutrophils and macrophage infiltrating the lesions. Myeloperoxidase (MPO), but not elastase (ELA), activity oscillated among the groups during the SCC development. Levels of proinflammatory cytokines IL-1β, IL-18, Tumor Necrosis Factor (TNF)-α and Interferon (IFN)-γ were decreased in the tumor microenvironment in the absence of inflammasome proteins. These observations suggest a link between inflammasome function and SCC tumorigenesis, indicating an important role for inflammasome activation in the control of SCC development.
Fil: Gasparoto, Thais Helena. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; Brasil
Fil: Ervolino de Oliveira, Carine. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; Brasil
Fil: Thomazini de Freitas, Luisa. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; Brasil
Fil: Ramos Pinheiro, Claudia. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; Brasil
Fil: Issa Hori, Juliana. University of São Paulo. Faculdade de Medicina de Ribeirão Preto; Brasil
Fil: Pompermaier Garlet, Gustavo. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; Brasil
Fil: Cavassani, Karen Angélica. University Of Michigan; Estados Unidos
Fil: Schillaci, Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Santana Da Silva, Joao. University of São Paulo. Faculdade de Medicina de Ribeirão Preto; Brasil
Fil: Simmões Zamboni, Darío. University of São Paulo. Faculdade de Medicina de Ribeirão Preto; Brasil
Fil: Campanelli, Ana Paula. Universidad de São Paulo. Faculdade de Odontologia de Bauru. Departamento de Ciencias Biológicas; Brasil
description Chronic inflammation affects most stages of tumorigenesis, including initiation, promotion, malignant differentiation, invasion and metastasis. Inflammasomes have been described as involved with persistent inflammation and are known to exert both pro and antitumour effects. We evaluated the influence of apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and caspase (CASP)-1 in the antitumor immune response using a multistage model of squamous cell carcinoma (SCC) development. Absence of ASC and CASP-1 resulted in an earlier incidence and increased number of papilloma. Loss of inflammassome function in mice resulted in decreased presence of natural killer (NK), dendritic (DC), CD4+, CD8+ and CD45RB+ T cells in the tumor lesions as well as in lymph nodes (LN) compared with WT mice. Increased percentage of CD4+CD25+Foxp3+ T cells was associated with association with inflammasome loss of function. Moreover, significant differences were also found with neutrophils and macrophage infiltrating the lesions. Myeloperoxidase (MPO), but not elastase (ELA), activity oscillated among the groups during the SCC development. Levels of proinflammatory cytokines IL-1β, IL-18, Tumor Necrosis Factor (TNF)-α and Interferon (IFN)-γ were decreased in the tumor microenvironment in the absence of inflammasome proteins. These observations suggest a link between inflammasome function and SCC tumorigenesis, indicating an important role for inflammasome activation in the control of SCC development.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6438
Gasparoto, Thais Helena; Ervolino de Oliveira, Carine; Thomazini de Freitas, Luisa; Ramos Pinheiro, Claudia; Issa Hori, Juliana; et al.; Inflammasome activation is critical to the protective immune response during chemically induced squamous cell carcinoma; Public Library of Science; Plos One; 9; 9; 30-9-2014; e107170-e107170
1932-6203
1932-6203
url http://hdl.handle.net/11336/6438
identifier_str_mv Gasparoto, Thais Helena; Ervolino de Oliveira, Carine; Thomazini de Freitas, Luisa; Ramos Pinheiro, Claudia; Issa Hori, Juliana; et al.; Inflammasome activation is critical to the protective immune response during chemically induced squamous cell carcinoma; Public Library of Science; Plos One; 9; 9; 30-9-2014; e107170-e107170
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4182037/
info:eu-repo/semantics/altIdentifier/pmid/25268644
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0107170
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0107170
info:eu-repo/semantics/altIdentifier/pmid/PMC4182037
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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