Patch-Clamping Drosophila Brain Neurons

Autores
Fernandez Chiappe, Florencia; Muraro, Nara Ines
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Drosophila melanogaster is widely used as a model organism in all fields of biomedical research. In neuroscience, vast amounts of information have been gained using this little fly including the identification of neuronal circuits that regulate behaviors, the unraveling of their genetic underpinnings, and the molecular mechanisms involved. With plenty of genetic tools available to manipulate and infer neuronal activity, the direct measurement of electrical properties of fly neurons has lagged behind. This is due to the intricacies of performing electrical recordings in small cells such as fly central neurons. The patch-clamp technique offers the unique possibility of directly measuring the electrical properties of Drosophila neurons. This step-by-step protocol provides detailed advice for mastering this technique.
Fil: Fernandez Chiappe, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Muraro, Nara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Materia
DROSOPHILA MELANOGASTER
ELECTROHYSIOLOGY
PATCH-CLAMP
DISSCTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215592
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/215592
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Patch-Clamping Drosophila Brain NeuronsFernandez Chiappe, FlorenciaMuraro, Nara InesDROSOPHILA MELANOGASTERELECTROHYSIOLOGYPATCH-CLAMPDISSCTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Drosophila melanogaster is widely used as a model organism in all fields of biomedical research. In neuroscience, vast amounts of information have been gained using this little fly including the identification of neuronal circuits that regulate behaviors, the unraveling of their genetic underpinnings, and the molecular mechanisms involved. With plenty of genetic tools available to manipulate and infer neuronal activity, the direct measurement of electrical properties of fly neurons has lagged behind. This is due to the intricacies of performing electrical recordings in small cells such as fly central neurons. The patch-clamp technique offers the unique possibility of directly measuring the electrical properties of Drosophila neurons. This step-by-step protocol provides detailed advice for mastering this technique.Fil: Fernandez Chiappe, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Muraro, Nara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaCold Spring Harbor Laboratory Press2022-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215592Fernandez Chiappe, Florencia; Muraro, Nara Ines; Patch-Clamping Drosophila Brain Neurons; Cold Spring Harbor Laboratory Press; Cold Spring Harbor Protocols; 2022; 8; 8-2022; 392-4031940-34021559-6095CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://cshprotocols.cshlp.org/content/2022/8/pdb.prot107936.longinfo:eu-repo/semantics/altIdentifier/doi/10.1101/pdb.prot107936info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:40:21Zoai:ri.conicet.gov.ar:11336/215592instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:40:21.605CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Patch-Clamping Drosophila Brain Neurons
title Patch-Clamping Drosophila Brain Neurons
spellingShingle Patch-Clamping Drosophila Brain Neurons
Fernandez Chiappe, Florencia
DROSOPHILA MELANOGASTER
ELECTROHYSIOLOGY
PATCH-CLAMP
DISSCTION
title_short Patch-Clamping Drosophila Brain Neurons
title_full Patch-Clamping Drosophila Brain Neurons
title_fullStr Patch-Clamping Drosophila Brain Neurons
title_full_unstemmed Patch-Clamping Drosophila Brain Neurons
title_sort Patch-Clamping Drosophila Brain Neurons
dc.creator.none.fl_str_mv Fernandez Chiappe, Florencia
Muraro, Nara Ines
author Fernandez Chiappe, Florencia
author_facet Fernandez Chiappe, Florencia
Muraro, Nara Ines
author_role author
author2 Muraro, Nara Ines
author2_role author
dc.subject.none.fl_str_mv DROSOPHILA MELANOGASTER
ELECTROHYSIOLOGY
PATCH-CLAMP
DISSCTION
topic DROSOPHILA MELANOGASTER
ELECTROHYSIOLOGY
PATCH-CLAMP
DISSCTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Drosophila melanogaster is widely used as a model organism in all fields of biomedical research. In neuroscience, vast amounts of information have been gained using this little fly including the identification of neuronal circuits that regulate behaviors, the unraveling of their genetic underpinnings, and the molecular mechanisms involved. With plenty of genetic tools available to manipulate and infer neuronal activity, the direct measurement of electrical properties of fly neurons has lagged behind. This is due to the intricacies of performing electrical recordings in small cells such as fly central neurons. The patch-clamp technique offers the unique possibility of directly measuring the electrical properties of Drosophila neurons. This step-by-step protocol provides detailed advice for mastering this technique.
Fil: Fernandez Chiappe, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Muraro, Nara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
description Drosophila melanogaster is widely used as a model organism in all fields of biomedical research. In neuroscience, vast amounts of information have been gained using this little fly including the identification of neuronal circuits that regulate behaviors, the unraveling of their genetic underpinnings, and the molecular mechanisms involved. With plenty of genetic tools available to manipulate and infer neuronal activity, the direct measurement of electrical properties of fly neurons has lagged behind. This is due to the intricacies of performing electrical recordings in small cells such as fly central neurons. The patch-clamp technique offers the unique possibility of directly measuring the electrical properties of Drosophila neurons. This step-by-step protocol provides detailed advice for mastering this technique.
publishDate 2022
dc.date.none.fl_str_mv 2022-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215592
Fernandez Chiappe, Florencia; Muraro, Nara Ines; Patch-Clamping Drosophila Brain Neurons; Cold Spring Harbor Laboratory Press; Cold Spring Harbor Protocols; 2022; 8; 8-2022; 392-403
1940-3402
1559-6095
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215592
identifier_str_mv Fernandez Chiappe, Florencia; Muraro, Nara Ines; Patch-Clamping Drosophila Brain Neurons; Cold Spring Harbor Laboratory Press; Cold Spring Harbor Protocols; 2022; 8; 8-2022; 392-403
1940-3402
1559-6095
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://cshprotocols.cshlp.org/content/2022/8/pdb.prot107936.long
info:eu-repo/semantics/altIdentifier/doi/10.1101/pdb.prot107936
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083517986897920
score 13.221938

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