Dissection of Drosophila Adult Brains for Patch-Clamping Neurons

Autores
Fernandez Chiappe, Florencia; Muraro, Nara Ines
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The brain of adult flies (Drosophila melanogaster) has been studied in detail from several perspectives, including the anatomical and molecular characterization of hundreds of neuronal types. However, information regarding the electrophysiological properties of most neuronal types is lacking. This protocol provides detailed information on how to dissect the brain of adult flies to produce an ex vivo preparation in which central neurons can be patch-clamped. Immobilizing fresh and tiny tissues, such as fly brains, to perform successful patch-clamp recordings is a critical step; here, we explain how this can be achieved using cyanoacrylate glue.
Fil: Fernandez Chiappe, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Muraro, Nara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Materia
DROSOPHILA MELANOGASTER
ELECTROPHYSIOLOGY
PATCH-CLAMP
NEUROSCIENCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215590
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Dissection of Drosophila Adult Brains for Patch-Clamping NeuronsFernandez Chiappe, FlorenciaMuraro, Nara InesDROSOPHILA MELANOGASTERELECTROPHYSIOLOGYPATCH-CLAMPNEUROSCIENCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The brain of adult flies (Drosophila melanogaster) has been studied in detail from several perspectives, including the anatomical and molecular characterization of hundreds of neuronal types. However, information regarding the electrophysiological properties of most neuronal types is lacking. This protocol provides detailed information on how to dissect the brain of adult flies to produce an ex vivo preparation in which central neurons can be patch-clamped. Immobilizing fresh and tiny tissues, such as fly brains, to perform successful patch-clamp recordings is a critical step; here, we explain how this can be achieved using cyanoacrylate glue.Fil: Fernandez Chiappe, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Muraro, Nara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaCold Spring Harbor Laboratory Press2022-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215590Fernandez Chiappe, Florencia; Muraro, Nara Ines; Dissection of Drosophila Adult Brains for Patch-Clamping Neurons; Cold Spring Harbor Laboratory Press; Cold Spring Harbor Protocols; 2022; 8; 8-2022; 385-3911940-34021559-6095CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://cshprotocols.cshlp.org/content/2022/8/pdb.prot107935.longinfo:eu-repo/semantics/altIdentifier/doi/10.1101/pdb.prot107935info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:49Zoai:ri.conicet.gov.ar:11336/215590instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:49.623CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dissection of Drosophila Adult Brains for Patch-Clamping Neurons
title Dissection of Drosophila Adult Brains for Patch-Clamping Neurons
spellingShingle Dissection of Drosophila Adult Brains for Patch-Clamping Neurons
Fernandez Chiappe, Florencia
DROSOPHILA MELANOGASTER
ELECTROPHYSIOLOGY
PATCH-CLAMP
NEUROSCIENCE
title_short Dissection of Drosophila Adult Brains for Patch-Clamping Neurons
title_full Dissection of Drosophila Adult Brains for Patch-Clamping Neurons
title_fullStr Dissection of Drosophila Adult Brains for Patch-Clamping Neurons
title_full_unstemmed Dissection of Drosophila Adult Brains for Patch-Clamping Neurons
title_sort Dissection of Drosophila Adult Brains for Patch-Clamping Neurons
dc.creator.none.fl_str_mv Fernandez Chiappe, Florencia
Muraro, Nara Ines
author Fernandez Chiappe, Florencia
author_facet Fernandez Chiappe, Florencia
Muraro, Nara Ines
author_role author
author2 Muraro, Nara Ines
author2_role author
dc.subject.none.fl_str_mv DROSOPHILA MELANOGASTER
ELECTROPHYSIOLOGY
PATCH-CLAMP
NEUROSCIENCE
topic DROSOPHILA MELANOGASTER
ELECTROPHYSIOLOGY
PATCH-CLAMP
NEUROSCIENCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The brain of adult flies (Drosophila melanogaster) has been studied in detail from several perspectives, including the anatomical and molecular characterization of hundreds of neuronal types. However, information regarding the electrophysiological properties of most neuronal types is lacking. This protocol provides detailed information on how to dissect the brain of adult flies to produce an ex vivo preparation in which central neurons can be patch-clamped. Immobilizing fresh and tiny tissues, such as fly brains, to perform successful patch-clamp recordings is a critical step; here, we explain how this can be achieved using cyanoacrylate glue.
Fil: Fernandez Chiappe, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Muraro, Nara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
description The brain of adult flies (Drosophila melanogaster) has been studied in detail from several perspectives, including the anatomical and molecular characterization of hundreds of neuronal types. However, information regarding the electrophysiological properties of most neuronal types is lacking. This protocol provides detailed information on how to dissect the brain of adult flies to produce an ex vivo preparation in which central neurons can be patch-clamped. Immobilizing fresh and tiny tissues, such as fly brains, to perform successful patch-clamp recordings is a critical step; here, we explain how this can be achieved using cyanoacrylate glue.
publishDate 2022
dc.date.none.fl_str_mv 2022-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215590
Fernandez Chiappe, Florencia; Muraro, Nara Ines; Dissection of Drosophila Adult Brains for Patch-Clamping Neurons; Cold Spring Harbor Laboratory Press; Cold Spring Harbor Protocols; 2022; 8; 8-2022; 385-391
1940-3402
1559-6095
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215590
identifier_str_mv Fernandez Chiappe, Florencia; Muraro, Nara Ines; Dissection of Drosophila Adult Brains for Patch-Clamping Neurons; Cold Spring Harbor Laboratory Press; Cold Spring Harbor Protocols; 2022; 8; 8-2022; 385-391
1940-3402
1559-6095
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://cshprotocols.cshlp.org/content/2022/8/pdb.prot107935.long
info:eu-repo/semantics/altIdentifier/doi/10.1101/pdb.prot107935
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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