Dissection of Drosophila Wandering Larval Brains for Patch-Clamping Neurons

Autores
Fernandez Chiappe, Florencia; Muraro, Nara Ines
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An enormous amount of neuroscientific knowledge has been gained from studying the larval stage of Drosophila From an electrophysiological point of view, the larval neuromuscular junction has played an important role in this quest for knowledge, as it presents practical advantages such as accessibility and a stereotypic pattern. The physiological properties of larval central neurons have been less explored, with information regarding mainly a few identified motoneurons available to date. This protocol describes a quick and easy dissection of the brain of wandering third-instar Drosophila larvae to produce an ex vivo preparation in which central neurons can be patch-clamped. Immobilizing fresh and tiny tissues, such as larval brains, to perform successful patch-clamp recordings is a crucial step; here we explain in detail how this can be achieved using cyanoacrylate glue.
Fil: Fernandez Chiappe, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Muraro, Nara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Materia
DROSOPHILA MELANOGASTER
ELECTROPHYSIOLOGY
PATCH-CLAMP
DISSECTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/215596
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Dissection of Drosophila Wandering Larval Brains for Patch-Clamping NeuronsFernandez Chiappe, FlorenciaMuraro, Nara InesDROSOPHILA MELANOGASTERELECTROPHYSIOLOGYPATCH-CLAMPDISSECTIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1An enormous amount of neuroscientific knowledge has been gained from studying the larval stage of Drosophila From an electrophysiological point of view, the larval neuromuscular junction has played an important role in this quest for knowledge, as it presents practical advantages such as accessibility and a stereotypic pattern. The physiological properties of larval central neurons have been less explored, with information regarding mainly a few identified motoneurons available to date. This protocol describes a quick and easy dissection of the brain of wandering third-instar Drosophila larvae to produce an ex vivo preparation in which central neurons can be patch-clamped. Immobilizing fresh and tiny tissues, such as larval brains, to perform successful patch-clamp recordings is a crucial step; here we explain in detail how this can be achieved using cyanoacrylate glue.Fil: Fernandez Chiappe, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaFil: Muraro, Nara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; ArgentinaCold Spring Harbor Laboratory Press2022-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/215596Fernandez Chiappe, Florencia; Muraro, Nara Ines; Dissection of Drosophila Wandering Larval Brains for Patch-Clamping Neurons; Cold Spring Harbor Laboratory Press; Cold Spring Harbor Protocols; 2022; 8; 8-2022; 379-3841940-34021559-6095CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://cshprotocols.cshlp.org/content/2022/8/pdb.prot107934.longinfo:eu-repo/semantics/altIdentifier/doi/10.1101/pdb.prot107934info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:11:55Zoai:ri.conicet.gov.ar:11336/215596instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:11:56.264CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dissection of Drosophila Wandering Larval Brains for Patch-Clamping Neurons
title Dissection of Drosophila Wandering Larval Brains for Patch-Clamping Neurons
spellingShingle Dissection of Drosophila Wandering Larval Brains for Patch-Clamping Neurons
Fernandez Chiappe, Florencia
DROSOPHILA MELANOGASTER
ELECTROPHYSIOLOGY
PATCH-CLAMP
DISSECTION
title_short Dissection of Drosophila Wandering Larval Brains for Patch-Clamping Neurons
title_full Dissection of Drosophila Wandering Larval Brains for Patch-Clamping Neurons
title_fullStr Dissection of Drosophila Wandering Larval Brains for Patch-Clamping Neurons
title_full_unstemmed Dissection of Drosophila Wandering Larval Brains for Patch-Clamping Neurons
title_sort Dissection of Drosophila Wandering Larval Brains for Patch-Clamping Neurons
dc.creator.none.fl_str_mv Fernandez Chiappe, Florencia
Muraro, Nara Ines
author Fernandez Chiappe, Florencia
author_facet Fernandez Chiappe, Florencia
Muraro, Nara Ines
author_role author
author2 Muraro, Nara Ines
author2_role author
dc.subject.none.fl_str_mv DROSOPHILA MELANOGASTER
ELECTROPHYSIOLOGY
PATCH-CLAMP
DISSECTION
topic DROSOPHILA MELANOGASTER
ELECTROPHYSIOLOGY
PATCH-CLAMP
DISSECTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An enormous amount of neuroscientific knowledge has been gained from studying the larval stage of Drosophila From an electrophysiological point of view, the larval neuromuscular junction has played an important role in this quest for knowledge, as it presents practical advantages such as accessibility and a stereotypic pattern. The physiological properties of larval central neurons have been less explored, with information regarding mainly a few identified motoneurons available to date. This protocol describes a quick and easy dissection of the brain of wandering third-instar Drosophila larvae to produce an ex vivo preparation in which central neurons can be patch-clamped. Immobilizing fresh and tiny tissues, such as larval brains, to perform successful patch-clamp recordings is a crucial step; here we explain in detail how this can be achieved using cyanoacrylate glue.
Fil: Fernandez Chiappe, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
Fil: Muraro, Nara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina
description An enormous amount of neuroscientific knowledge has been gained from studying the larval stage of Drosophila From an electrophysiological point of view, the larval neuromuscular junction has played an important role in this quest for knowledge, as it presents practical advantages such as accessibility and a stereotypic pattern. The physiological properties of larval central neurons have been less explored, with information regarding mainly a few identified motoneurons available to date. This protocol describes a quick and easy dissection of the brain of wandering third-instar Drosophila larvae to produce an ex vivo preparation in which central neurons can be patch-clamped. Immobilizing fresh and tiny tissues, such as larval brains, to perform successful patch-clamp recordings is a crucial step; here we explain in detail how this can be achieved using cyanoacrylate glue.
publishDate 2022
dc.date.none.fl_str_mv 2022-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/215596
Fernandez Chiappe, Florencia; Muraro, Nara Ines; Dissection of Drosophila Wandering Larval Brains for Patch-Clamping Neurons; Cold Spring Harbor Laboratory Press; Cold Spring Harbor Protocols; 2022; 8; 8-2022; 379-384
1940-3402
1559-6095
CONICET Digital
CONICET
url http://hdl.handle.net/11336/215596
identifier_str_mv Fernandez Chiappe, Florencia; Muraro, Nara Ines; Dissection of Drosophila Wandering Larval Brains for Patch-Clamping Neurons; Cold Spring Harbor Laboratory Press; Cold Spring Harbor Protocols; 2022; 8; 8-2022; 379-384
1940-3402
1559-6095
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://cshprotocols.cshlp.org/content/2022/8/pdb.prot107934.long
info:eu-repo/semantics/altIdentifier/doi/10.1101/pdb.prot107934
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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