Evaluation of the immunoregulatory capacities of feed microbial materials in porcine intestinal immune and epithelial cells.

Autores
Kumagae, Naosuke; Villena, Julio Cesar; Tomosada, Yohsuke; Kobayashi, Hisakazu; Kanmani, Paulraj; Aso, Hisashi; Sasaki, Takashi; Yoshida, Motohiko; Tanabe, Hiroshi; Shibata, Isao; Saito, Tadao; Kitazawa, Haruki
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The establishment of drug-free feeding systems has been required for secure and healthy lives- tock production. Although functional feed materials containing microorganisms as alternatives to enhance intestinal immunity are expected to be beneficial for reducing diarrhoea caused by pathogens in weaned piglets, the effects of such materials on porcine intestinal cells have not been investigated in detail. Therefore, this work evaluated the immunoregulatory functions of microbial feed materials in porcine intestinal immune and epithelial cells. Porcine immune cells isolated from Peyer?s patches and mesenteric lymph nodes were stimulated with six different feed materials containing microorganisms, and evaluated for lymphocyte mitogenicity and cytokine inductions. In addition, porcine intestinal epithelial cells were stimulated with the materials before treatment with heat-killed enterotoxigenic Escherichia coli (ETEC), and analyzed for the proinflammatory cytokine expressions. The material containing Bifidobacterium thermophilum significantly augmented lymphocytes? mitogenicity and also induced a high expression of IL-2, IL-6 and IFN-γ in immune cells, and inhibited ETEC-induced overexpression of IL-6 and IL-8 via regulation of Toll-like receptor signaling. These results suggest that this feed material stimulates intestinal epithelial and immune cells to exert immunoregulation, suggesting that this feed is expected to contribute to promoting the health of piglets without using antimicrobial feed materials.
Fil: Kumagae, Naosuke. Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; Japón. Scientific Feed Laboratory Co. Ltd.; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; Japón
Fil: Tomosada, Yohsuke. Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; Japón
Fil: Kobayashi, Hisakazu . Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; Japón
Fil: Kanmani, Paulraj. Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; Japón. Japan Society for the Promotion of Science; Japón
Fil: Aso, Hisashi . Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Sasaki, Takashi . Scientific Feed Laboratory Co. Ltd.; Japón
Fil: Yoshida, Motohiko . Scientific Feed Laboratory Co. Ltd.; Japón
Fil: Tanabe, Hiroshi. Scientific Feed Laboratory Co. Ltd.; Japón
Fil: Shibata, Isao. Scientific Feed Laboratory Co. Ltd.; Japón
Fil: Saito, Tadao . Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; Japón
Fil: Kitazawa, Haruki. Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; Japón
Materia
FEED MICROBIAL MATERIAL
IMMUNOREGULATORY EFFECT
PORCINE INTESTINAL EPITHELIOCYTES
PORCINE IMMUNE CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2777
Acceder
id CONICETDig_56aebf23e5d1df8782b266a60187d117
oai_identifier_str oai:ri.conicet.gov.ar:11336/2777
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of the immunoregulatory capacities of feed microbial materials in porcine intestinal immune and epithelial cells.Kumagae, NaosukeVillena, Julio CesarTomosada, YohsukeKobayashi, Hisakazu Kanmani, PaulrajAso, Hisashi Sasaki, Takashi Yoshida, Motohiko Tanabe, HiroshiShibata, IsaoSaito, Tadao Kitazawa, HarukiFEED MICROBIAL MATERIALIMMUNOREGULATORY EFFECTPORCINE INTESTINAL EPITHELIOCYTESPORCINE IMMUNE CELLShttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The establishment of drug-free feeding systems has been required for secure and healthy lives- tock production. Although functional feed materials containing microorganisms as alternatives to enhance intestinal immunity are expected to be beneficial for reducing diarrhoea caused by pathogens in weaned piglets, the effects of such materials on porcine intestinal cells have not been investigated in detail. Therefore, this work evaluated the immunoregulatory functions of microbial feed materials in porcine intestinal immune and epithelial cells. Porcine immune cells isolated from Peyer?s patches and mesenteric lymph nodes were stimulated with six different feed materials containing microorganisms, and evaluated for lymphocyte mitogenicity and cytokine inductions. In addition, porcine intestinal epithelial cells were stimulated with the materials before treatment with heat-killed enterotoxigenic Escherichia coli (ETEC), and analyzed for the proinflammatory cytokine expressions. The material containing Bifidobacterium thermophilum significantly augmented lymphocytes? mitogenicity and also induced a high expression of IL-2, IL-6 and IFN-γ in immune cells, and inhibited ETEC-induced overexpression of IL-6 and IL-8 via regulation of Toll-like receptor signaling. These results suggest that this feed material stimulates intestinal epithelial and immune cells to exert immunoregulation, suggesting that this feed is expected to contribute to promoting the health of piglets without using antimicrobial feed materials.Fil: Kumagae, Naosuke. Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; Japón. Scientific Feed Laboratory Co. Ltd.; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; JapónFil: Tomosada, Yohsuke. Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; JapónFil: Kobayashi, Hisakazu . Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; JapónFil: Kanmani, Paulraj. Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; Japón. Japan Society for the Promotion of Science; JapónFil: Aso, Hisashi . Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; JapónFil: Sasaki, Takashi . Scientific Feed Laboratory Co. Ltd.; JapónFil: Yoshida, Motohiko . Scientific Feed Laboratory Co. Ltd.; JapónFil: Tanabe, Hiroshi. Scientific Feed Laboratory Co. Ltd.; JapónFil: Shibata, Isao. Scientific Feed Laboratory Co. Ltd.; JapónFil: Saito, Tadao . Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; JapónFil: Kitazawa, Haruki. Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; JapónScientific Research2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2777Kumagae, Naosuke; Villena, Julio Cesar; Tomosada, Yohsuke; Kobayashi, Hisakazu ; Kanmani, Paulraj; et al.; Evaluation of the immunoregulatory capacities of feed microbial materials in porcine intestinal immune and epithelial cells.; Scientific Research; Open Journal of Veterinary Medicine; 4; 3; 3-2014; 15-282165-33562165-3364enginfo:eu-repo/semantics/altIdentifier/doi/10.4236/ojvm.2014.43003info:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/Journal/Home.aspx?IssueID=4563#.VkTqc9IrJkginfo:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/ojvminfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-08T12:52:48Zoai:ri.conicet.gov.ar:11336/2777instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-08 12:52:48.375CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of the immunoregulatory capacities of feed microbial materials in porcine intestinal immune and epithelial cells.
title Evaluation of the immunoregulatory capacities of feed microbial materials in porcine intestinal immune and epithelial cells.
spellingShingle Evaluation of the immunoregulatory capacities of feed microbial materials in porcine intestinal immune and epithelial cells.
Kumagae, Naosuke
FEED MICROBIAL MATERIAL
IMMUNOREGULATORY EFFECT
PORCINE INTESTINAL EPITHELIOCYTES
PORCINE IMMUNE CELLS
title_short Evaluation of the immunoregulatory capacities of feed microbial materials in porcine intestinal immune and epithelial cells.
title_full Evaluation of the immunoregulatory capacities of feed microbial materials in porcine intestinal immune and epithelial cells.
title_fullStr Evaluation of the immunoregulatory capacities of feed microbial materials in porcine intestinal immune and epithelial cells.
title_full_unstemmed Evaluation of the immunoregulatory capacities of feed microbial materials in porcine intestinal immune and epithelial cells.
title_sort Evaluation of the immunoregulatory capacities of feed microbial materials in porcine intestinal immune and epithelial cells.
dc.creator.none.fl_str_mv Kumagae, Naosuke
Villena, Julio Cesar
Tomosada, Yohsuke
Kobayashi, Hisakazu
Kanmani, Paulraj
Aso, Hisashi
Sasaki, Takashi
Yoshida, Motohiko
Tanabe, Hiroshi
Shibata, Isao
Saito, Tadao
Kitazawa, Haruki
author Kumagae, Naosuke
author_facet Kumagae, Naosuke
Villena, Julio Cesar
Tomosada, Yohsuke
Kobayashi, Hisakazu
Kanmani, Paulraj
Aso, Hisashi
Sasaki, Takashi
Yoshida, Motohiko
Tanabe, Hiroshi
Shibata, Isao
Saito, Tadao
Kitazawa, Haruki
author_role author
author2 Villena, Julio Cesar
Tomosada, Yohsuke
Kobayashi, Hisakazu
Kanmani, Paulraj
Aso, Hisashi
Sasaki, Takashi
Yoshida, Motohiko
Tanabe, Hiroshi
Shibata, Isao
Saito, Tadao
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FEED MICROBIAL MATERIAL
IMMUNOREGULATORY EFFECT
PORCINE INTESTINAL EPITHELIOCYTES
PORCINE IMMUNE CELLS
topic FEED MICROBIAL MATERIAL
IMMUNOREGULATORY EFFECT
PORCINE INTESTINAL EPITHELIOCYTES
PORCINE IMMUNE CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The establishment of drug-free feeding systems has been required for secure and healthy lives- tock production. Although functional feed materials containing microorganisms as alternatives to enhance intestinal immunity are expected to be beneficial for reducing diarrhoea caused by pathogens in weaned piglets, the effects of such materials on porcine intestinal cells have not been investigated in detail. Therefore, this work evaluated the immunoregulatory functions of microbial feed materials in porcine intestinal immune and epithelial cells. Porcine immune cells isolated from Peyer?s patches and mesenteric lymph nodes were stimulated with six different feed materials containing microorganisms, and evaluated for lymphocyte mitogenicity and cytokine inductions. In addition, porcine intestinal epithelial cells were stimulated with the materials before treatment with heat-killed enterotoxigenic Escherichia coli (ETEC), and analyzed for the proinflammatory cytokine expressions. The material containing Bifidobacterium thermophilum significantly augmented lymphocytes? mitogenicity and also induced a high expression of IL-2, IL-6 and IFN-γ in immune cells, and inhibited ETEC-induced overexpression of IL-6 and IL-8 via regulation of Toll-like receptor signaling. These results suggest that this feed material stimulates intestinal epithelial and immune cells to exert immunoregulation, suggesting that this feed is expected to contribute to promoting the health of piglets without using antimicrobial feed materials.
Fil: Kumagae, Naosuke. Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; Japón. Scientific Feed Laboratory Co. Ltd.; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina. Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; Japón
Fil: Tomosada, Yohsuke. Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; Japón
Fil: Kobayashi, Hisakazu . Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; Japón
Fil: Kanmani, Paulraj. Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; Japón. Japan Society for the Promotion of Science; Japón
Fil: Aso, Hisashi . Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Sasaki, Takashi . Scientific Feed Laboratory Co. Ltd.; Japón
Fil: Yoshida, Motohiko . Scientific Feed Laboratory Co. Ltd.; Japón
Fil: Tanabe, Hiroshi. Scientific Feed Laboratory Co. Ltd.; Japón
Fil: Shibata, Isao. Scientific Feed Laboratory Co. Ltd.; Japón
Fil: Saito, Tadao . Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; Japón
Fil: Kitazawa, Haruki. Tohoku University. Graduate School of Agricultural Science. Laboratory of Animal Products Chemistry. Food and Feed Immunology Group; Japón
description The establishment of drug-free feeding systems has been required for secure and healthy lives- tock production. Although functional feed materials containing microorganisms as alternatives to enhance intestinal immunity are expected to be beneficial for reducing diarrhoea caused by pathogens in weaned piglets, the effects of such materials on porcine intestinal cells have not been investigated in detail. Therefore, this work evaluated the immunoregulatory functions of microbial feed materials in porcine intestinal immune and epithelial cells. Porcine immune cells isolated from Peyer?s patches and mesenteric lymph nodes were stimulated with six different feed materials containing microorganisms, and evaluated for lymphocyte mitogenicity and cytokine inductions. In addition, porcine intestinal epithelial cells were stimulated with the materials before treatment with heat-killed enterotoxigenic Escherichia coli (ETEC), and analyzed for the proinflammatory cytokine expressions. The material containing Bifidobacterium thermophilum significantly augmented lymphocytes? mitogenicity and also induced a high expression of IL-2, IL-6 and IFN-γ in immune cells, and inhibited ETEC-induced overexpression of IL-6 and IL-8 via regulation of Toll-like receptor signaling. These results suggest that this feed material stimulates intestinal epithelial and immune cells to exert immunoregulation, suggesting that this feed is expected to contribute to promoting the health of piglets without using antimicrobial feed materials.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2777
Kumagae, Naosuke; Villena, Julio Cesar; Tomosada, Yohsuke; Kobayashi, Hisakazu ; Kanmani, Paulraj; et al.; Evaluation of the immunoregulatory capacities of feed microbial materials in porcine intestinal immune and epithelial cells.; Scientific Research; Open Journal of Veterinary Medicine; 4; 3; 3-2014; 15-28
2165-3356
2165-3364
url http://hdl.handle.net/11336/2777
identifier_str_mv Kumagae, Naosuke; Villena, Julio Cesar; Tomosada, Yohsuke; Kobayashi, Hisakazu ; Kanmani, Paulraj; et al.; Evaluation of the immunoregulatory capacities of feed microbial materials in porcine intestinal immune and epithelial cells.; Scientific Research; Open Journal of Veterinary Medicine; 4; 3; 3-2014; 15-28
2165-3356
2165-3364
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4236/ojvm.2014.43003
info:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/Journal/Home.aspx?IssueID=4563#.VkTqc9IrJkg
info:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/ojvm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Research
publisher.none.fl_str_mv Scientific Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1853775269058314240
score 13.25844

Publicaciones similares