Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells

Autores
Hosoya, Soichi; Villena, Julio Cesar; Shimazu, Tomoyuki; Tohno, Masanori; Fujie, Hitomi; Chiba, Eriko; Shimosato, Takeshi; Aso, Hisashi; Suda, Yoshihito; Kawai, Yasushi; Saito, Tadao; Alvarez, Gladis Susana; Ikegami, Shuji; Itoh, Hiroyuki; Kitazawa, Haruki
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study analyzed the functional expression of TLR3 in various gastrointestinal tissues from adult swine and shows that TLR3 is expressed preferentially in intestinal epithelial cells (IEC), CD172a +CD11R1 high and CD4 + cells from ileal Peyer's patches. We characterized the inflammatory immune response triggered by TLR3 activation in a clonal porcine intestinal epitheliocyte cell line (PIE cells) and in PIE-immune cell co-cultures, and demonstrated that these systems are valuable tools to study in vitro the immune response triggered by TLR3 on IEC and the interaction between IEC and immune cells. In addition, we selected an immunobiotic lactic acid bacteria strain, Lactobacillus casei MEP221106, able to beneficially regulate the anti-viral immune response triggered by poly(I:C) stimulation in PIE cells. Moreover, we deepened our understanding of the possible mechanisms of immunobiotic action by demonstrating that L. casei MEP221106 modulates the interaction between IEC and immune cells during the generation of a TLR3-mediated immune response.
Fil: Hosoya, Soichi. Tohoku University. Food Immunology Group; Japón
Fil: Villena, Julio Cesar. Tohoku University. Food Immunology Group; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Shimazu, Tomoyuki. Tohoku University. Food Immunology Group; Japón
Fil: Tohno, Masanori. Tohoku University. Food Immunology Group; Japón
Fil: Fujie, Hitomi. Tohoku University. Food Immunology Group; Japón
Fil: Chiba, Eriko. Tohoku University. Food Immunology Group; Japón
Fil: Shimosato, Takeshi. Tohoku University. Food Immunology Group; Japón
Fil: Aso, Hisashi. Tohoku University. Food Immunology Group; Japón
Fil: Suda, Yoshihito. Miyagi University. Department of Food Agriculture; Japón
Fil: Kawai, Yasushi. Tohoku University. Food Immunology Group; Japón
Fil: Saito, Tadao. Tohoku University. Food Immunology Group; Japón
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Aplicada; Argentina
Fil: Ikegami, Shuji. Meiji Dairies Corporation Japan; Japón
Fil: Itoh, Hiroyuki. Meiji Dairies Corporation Japan; Japón
Fil: Kitazawa, Haruki. Tohoku University. Food Immunology Group; Japón
Materia
Porcine Intestinal Epithelial Cells
Inflammatory Response
Immunobiotics
Poly(I:C)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/54793
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/54793
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cellsHosoya, SoichiVillena, Julio CesarShimazu, TomoyukiTohno, MasanoriFujie, HitomiChiba, ErikoShimosato, TakeshiAso, HisashiSuda, YoshihitoKawai, YasushiSaito, TadaoAlvarez, Gladis SusanaIkegami, ShujiItoh, HiroyukiKitazawa, HarukiPorcine Intestinal Epithelial CellsInflammatory ResponseImmunobioticsPoly(I:C)https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4This study analyzed the functional expression of TLR3 in various gastrointestinal tissues from adult swine and shows that TLR3 is expressed preferentially in intestinal epithelial cells (IEC), CD172a +CD11R1 high and CD4 + cells from ileal Peyer's patches. We characterized the inflammatory immune response triggered by TLR3 activation in a clonal porcine intestinal epitheliocyte cell line (PIE cells) and in PIE-immune cell co-cultures, and demonstrated that these systems are valuable tools to study in vitro the immune response triggered by TLR3 on IEC and the interaction between IEC and immune cells. In addition, we selected an immunobiotic lactic acid bacteria strain, Lactobacillus casei MEP221106, able to beneficially regulate the anti-viral immune response triggered by poly(I:C) stimulation in PIE cells. Moreover, we deepened our understanding of the possible mechanisms of immunobiotic action by demonstrating that L. casei MEP221106 modulates the interaction between IEC and immune cells during the generation of a TLR3-mediated immune response.Fil: Hosoya, Soichi. Tohoku University. Food Immunology Group; JapónFil: Villena, Julio Cesar. Tohoku University. Food Immunology Group; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Shimazu, Tomoyuki. Tohoku University. Food Immunology Group; JapónFil: Tohno, Masanori. Tohoku University. Food Immunology Group; JapónFil: Fujie, Hitomi. Tohoku University. Food Immunology Group; JapónFil: Chiba, Eriko. Tohoku University. Food Immunology Group; JapónFil: Shimosato, Takeshi. Tohoku University. Food Immunology Group; JapónFil: Aso, Hisashi. Tohoku University. Food Immunology Group; JapónFil: Suda, Yoshihito. Miyagi University. Department of Food Agriculture; JapónFil: Kawai, Yasushi. Tohoku University. Food Immunology Group; JapónFil: Saito, Tadao. Tohoku University. Food Immunology Group; JapónFil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Aplicada; ArgentinaFil: Ikegami, Shuji. Meiji Dairies Corporation Japan; JapónFil: Itoh, Hiroyuki. Meiji Dairies Corporation Japan; JapónFil: Kitazawa, Haruki. Tohoku University. Food Immunology Group; JapónBioMed Central2011-11-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/54793Hosoya, Soichi; Villena, Julio Cesar; Shimazu, Tomoyuki; Tohno, Masanori; Fujie, Hitomi; et al.; Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells; BioMed Central; Veterinary Research; 42; 1; 3-11-2011; 1-13; 1110928-42491297-9716CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/1297-9716-42-111info:eu-repo/semantics/altIdentifier/url/https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-42-111info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:27Zoai:ri.conicet.gov.ar:11336/54793instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:27.375CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells
title Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells
spellingShingle Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells
Hosoya, Soichi
Porcine Intestinal Epithelial Cells
Inflammatory Response
Immunobiotics
Poly(I:C)
title_short Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells
title_full Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells
title_fullStr Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells
title_full_unstemmed Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells
title_sort Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells
dc.creator.none.fl_str_mv Hosoya, Soichi
Villena, Julio Cesar
Shimazu, Tomoyuki
Tohno, Masanori
Fujie, Hitomi
Chiba, Eriko
Shimosato, Takeshi
Aso, Hisashi
Suda, Yoshihito
Kawai, Yasushi
Saito, Tadao
Alvarez, Gladis Susana
Ikegami, Shuji
Itoh, Hiroyuki
Kitazawa, Haruki
author Hosoya, Soichi
author_facet Hosoya, Soichi
Villena, Julio Cesar
Shimazu, Tomoyuki
Tohno, Masanori
Fujie, Hitomi
Chiba, Eriko
Shimosato, Takeshi
Aso, Hisashi
Suda, Yoshihito
Kawai, Yasushi
Saito, Tadao
Alvarez, Gladis Susana
Ikegami, Shuji
Itoh, Hiroyuki
Kitazawa, Haruki
author_role author
author2 Villena, Julio Cesar
Shimazu, Tomoyuki
Tohno, Masanori
Fujie, Hitomi
Chiba, Eriko
Shimosato, Takeshi
Aso, Hisashi
Suda, Yoshihito
Kawai, Yasushi
Saito, Tadao
Alvarez, Gladis Susana
Ikegami, Shuji
Itoh, Hiroyuki
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Porcine Intestinal Epithelial Cells
Inflammatory Response
Immunobiotics
Poly(I:C)
topic Porcine Intestinal Epithelial Cells
Inflammatory Response
Immunobiotics
Poly(I:C)
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv This study analyzed the functional expression of TLR3 in various gastrointestinal tissues from adult swine and shows that TLR3 is expressed preferentially in intestinal epithelial cells (IEC), CD172a +CD11R1 high and CD4 + cells from ileal Peyer's patches. We characterized the inflammatory immune response triggered by TLR3 activation in a clonal porcine intestinal epitheliocyte cell line (PIE cells) and in PIE-immune cell co-cultures, and demonstrated that these systems are valuable tools to study in vitro the immune response triggered by TLR3 on IEC and the interaction between IEC and immune cells. In addition, we selected an immunobiotic lactic acid bacteria strain, Lactobacillus casei MEP221106, able to beneficially regulate the anti-viral immune response triggered by poly(I:C) stimulation in PIE cells. Moreover, we deepened our understanding of the possible mechanisms of immunobiotic action by demonstrating that L. casei MEP221106 modulates the interaction between IEC and immune cells during the generation of a TLR3-mediated immune response.
Fil: Hosoya, Soichi. Tohoku University. Food Immunology Group; Japón
Fil: Villena, Julio Cesar. Tohoku University. Food Immunology Group; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Shimazu, Tomoyuki. Tohoku University. Food Immunology Group; Japón
Fil: Tohno, Masanori. Tohoku University. Food Immunology Group; Japón
Fil: Fujie, Hitomi. Tohoku University. Food Immunology Group; Japón
Fil: Chiba, Eriko. Tohoku University. Food Immunology Group; Japón
Fil: Shimosato, Takeshi. Tohoku University. Food Immunology Group; Japón
Fil: Aso, Hisashi. Tohoku University. Food Immunology Group; Japón
Fil: Suda, Yoshihito. Miyagi University. Department of Food Agriculture; Japón
Fil: Kawai, Yasushi. Tohoku University. Food Immunology Group; Japón
Fil: Saito, Tadao. Tohoku University. Food Immunology Group; Japón
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Aplicada; Argentina
Fil: Ikegami, Shuji. Meiji Dairies Corporation Japan; Japón
Fil: Itoh, Hiroyuki. Meiji Dairies Corporation Japan; Japón
Fil: Kitazawa, Haruki. Tohoku University. Food Immunology Group; Japón
description This study analyzed the functional expression of TLR3 in various gastrointestinal tissues from adult swine and shows that TLR3 is expressed preferentially in intestinal epithelial cells (IEC), CD172a +CD11R1 high and CD4 + cells from ileal Peyer's patches. We characterized the inflammatory immune response triggered by TLR3 activation in a clonal porcine intestinal epitheliocyte cell line (PIE cells) and in PIE-immune cell co-cultures, and demonstrated that these systems are valuable tools to study in vitro the immune response triggered by TLR3 on IEC and the interaction between IEC and immune cells. In addition, we selected an immunobiotic lactic acid bacteria strain, Lactobacillus casei MEP221106, able to beneficially regulate the anti-viral immune response triggered by poly(I:C) stimulation in PIE cells. Moreover, we deepened our understanding of the possible mechanisms of immunobiotic action by demonstrating that L. casei MEP221106 modulates the interaction between IEC and immune cells during the generation of a TLR3-mediated immune response.
publishDate 2011
dc.date.none.fl_str_mv 2011-11-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/54793
Hosoya, Soichi; Villena, Julio Cesar; Shimazu, Tomoyuki; Tohno, Masanori; Fujie, Hitomi; et al.; Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells; BioMed Central; Veterinary Research; 42; 1; 3-11-2011; 1-13; 111
0928-4249
1297-9716
CONICET Digital
CONICET
url http://hdl.handle.net/11336/54793
identifier_str_mv Hosoya, Soichi; Villena, Julio Cesar; Shimazu, Tomoyuki; Tohno, Masanori; Fujie, Hitomi; et al.; Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells; BioMed Central; Veterinary Research; 42; 1; 3-11-2011; 1-13; 111
0928-4249
1297-9716
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/1297-9716-42-111
info:eu-repo/semantics/altIdentifier/url/https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-42-111
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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