Neural modulation of foraging strategies in C. elegans

Autores
Blanco, Maria Gabriela; Zabala, Agustina; Alkema, Mark J.; De Rosa, María José; Rayes, Diego Hernán
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Despite the intermodulation between serotonergic and adrenergic signals is crucial throughout the animal kingdom, the molecular and cellular mechanisms underlying this interrelation are poorly understood. Given its simplicity and the highly conserved neurological pathways, Caenorhabditis elegans is a powerful model organism that can be used to provide insights into the neural circuits linking 5-TH and catecholamines. When food-deprived worms encounter food, 5-HT is released to slow-down their locomotion and to stimulate pharyngeal pumping. In contrast, Tyramine (TA) and Octopamine (OA), invertebrate counterparts for adrenaline and noradrenaline, stimulate locomotion and decrease pharyngeal pumping. We found that well-fed tdc-1 (unable to synthesize TA and OA) and tbh-1 (unable to synthesize OA) null mutants are hypersensitive to the slowing-down response upon food encounter, resembling starved worms. Moreover, exogenous TA and OA recover the increased slowing-down locomotion of tdc-1 null mutants, suggesting that both, TA and OA, antagonize 5HTdependent food-related behaviors. Furthermore, our calcium imaging recordings showed that the peak of activity of serotoninergic neurons (ADF and NSM) upon encountering food is significantly higher in a tdc-1 null mutant background. We also found that tdc-1 null mutants are hypersensitive to the paralysis produced by exogenous 5-HT. Therefore, we are using this assay to evaluate the paralysis of the different TA and OA null receptor mutants to identify which receptors are involved in this antagonism. Taken together, these results suggest that TA and OA counteract with 5- HT by driving opposing food-related behaviors and by inhibiting 5-HT release
Fil: Blanco, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Zabala, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Alkema, Mark J.. University Of Massachussets. Medical School. School Of Medicine. Departament Of Neurology; Estados Unidos
Fil: De Rosa, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
II Latin American Worm Meeting
Rosario
Argentina
Latin American Worm Meeting
Materia
C. elegans
biogenic amines
tyramine
crosstalk
serotonin
food-related behaviors
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195364
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/195364
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neural modulation of foraging strategies in C. elegansBlanco, Maria GabrielaZabala, AgustinaAlkema, Mark J.De Rosa, María JoséRayes, Diego HernánC. elegansbiogenic aminestyraminecrosstalkserotoninfood-related behaviorshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Despite the intermodulation between serotonergic and adrenergic signals is crucial throughout the animal kingdom, the molecular and cellular mechanisms underlying this interrelation are poorly understood. Given its simplicity and the highly conserved neurological pathways, Caenorhabditis elegans is a powerful model organism that can be used to provide insights into the neural circuits linking 5-TH and catecholamines. When food-deprived worms encounter food, 5-HT is released to slow-down their locomotion and to stimulate pharyngeal pumping. In contrast, Tyramine (TA) and Octopamine (OA), invertebrate counterparts for adrenaline and noradrenaline, stimulate locomotion and decrease pharyngeal pumping. We found that well-fed tdc-1 (unable to synthesize TA and OA) and tbh-1 (unable to synthesize OA) null mutants are hypersensitive to the slowing-down response upon food encounter, resembling starved worms. Moreover, exogenous TA and OA recover the increased slowing-down locomotion of tdc-1 null mutants, suggesting that both, TA and OA, antagonize 5HTdependent food-related behaviors. Furthermore, our calcium imaging recordings showed that the peak of activity of serotoninergic neurons (ADF and NSM) upon encountering food is significantly higher in a tdc-1 null mutant background. We also found that tdc-1 null mutants are hypersensitive to the paralysis produced by exogenous 5-HT. Therefore, we are using this assay to evaluate the paralysis of the different TA and OA null receptor mutants to identify which receptors are involved in this antagonism. Taken together, these results suggest that TA and OA counteract with 5- HT by driving opposing food-related behaviors and by inhibiting 5-HT releaseFil: Blanco, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Zabala, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Alkema, Mark J.. University Of Massachussets. Medical School. School Of Medicine. Departament Of Neurology; Estados UnidosFil: De Rosa, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaII Latin American Worm MeetingRosarioArgentinaLatin American Worm MeetingLatin American Worm Meeting2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195364Neural modulation of foraging strategies in C. elegans; II Latin American Worm Meeting; Rosario; Argentina; 2020; 17-17CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://acacimesfe.org/actividades/reuniones/reuniones-2020/2nd-latin-american-worm-meeting-february-19-21-2020-rosario/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:39:21Zoai:ri.conicet.gov.ar:11336/195364instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:39:21.659CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neural modulation of foraging strategies in C. elegans
title Neural modulation of foraging strategies in C. elegans
spellingShingle Neural modulation of foraging strategies in C. elegans
Blanco, Maria Gabriela
C. elegans
biogenic amines
tyramine
crosstalk
serotonin
food-related behaviors
title_short Neural modulation of foraging strategies in C. elegans
title_full Neural modulation of foraging strategies in C. elegans
title_fullStr Neural modulation of foraging strategies in C. elegans
title_full_unstemmed Neural modulation of foraging strategies in C. elegans
title_sort Neural modulation of foraging strategies in C. elegans
dc.creator.none.fl_str_mv Blanco, Maria Gabriela
Zabala, Agustina
Alkema, Mark J.
De Rosa, María José
Rayes, Diego Hernán
author Blanco, Maria Gabriela
author_facet Blanco, Maria Gabriela
Zabala, Agustina
Alkema, Mark J.
De Rosa, María José
Rayes, Diego Hernán
author_role author
author2 Zabala, Agustina
Alkema, Mark J.
De Rosa, María José
Rayes, Diego Hernán
author2_role author
author
author
author
dc.subject.none.fl_str_mv C. elegans
biogenic amines
tyramine
crosstalk
serotonin
food-related behaviors
topic C. elegans
biogenic amines
tyramine
crosstalk
serotonin
food-related behaviors
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Despite the intermodulation between serotonergic and adrenergic signals is crucial throughout the animal kingdom, the molecular and cellular mechanisms underlying this interrelation are poorly understood. Given its simplicity and the highly conserved neurological pathways, Caenorhabditis elegans is a powerful model organism that can be used to provide insights into the neural circuits linking 5-TH and catecholamines. When food-deprived worms encounter food, 5-HT is released to slow-down their locomotion and to stimulate pharyngeal pumping. In contrast, Tyramine (TA) and Octopamine (OA), invertebrate counterparts for adrenaline and noradrenaline, stimulate locomotion and decrease pharyngeal pumping. We found that well-fed tdc-1 (unable to synthesize TA and OA) and tbh-1 (unable to synthesize OA) null mutants are hypersensitive to the slowing-down response upon food encounter, resembling starved worms. Moreover, exogenous TA and OA recover the increased slowing-down locomotion of tdc-1 null mutants, suggesting that both, TA and OA, antagonize 5HTdependent food-related behaviors. Furthermore, our calcium imaging recordings showed that the peak of activity of serotoninergic neurons (ADF and NSM) upon encountering food is significantly higher in a tdc-1 null mutant background. We also found that tdc-1 null mutants are hypersensitive to the paralysis produced by exogenous 5-HT. Therefore, we are using this assay to evaluate the paralysis of the different TA and OA null receptor mutants to identify which receptors are involved in this antagonism. Taken together, these results suggest that TA and OA counteract with 5- HT by driving opposing food-related behaviors and by inhibiting 5-HT release
Fil: Blanco, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Zabala, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Alkema, Mark J.. University Of Massachussets. Medical School. School Of Medicine. Departament Of Neurology; Estados Unidos
Fil: De Rosa, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
II Latin American Worm Meeting
Rosario
Argentina
Latin American Worm Meeting
description Despite the intermodulation between serotonergic and adrenergic signals is crucial throughout the animal kingdom, the molecular and cellular mechanisms underlying this interrelation are poorly understood. Given its simplicity and the highly conserved neurological pathways, Caenorhabditis elegans is a powerful model organism that can be used to provide insights into the neural circuits linking 5-TH and catecholamines. When food-deprived worms encounter food, 5-HT is released to slow-down their locomotion and to stimulate pharyngeal pumping. In contrast, Tyramine (TA) and Octopamine (OA), invertebrate counterparts for adrenaline and noradrenaline, stimulate locomotion and decrease pharyngeal pumping. We found that well-fed tdc-1 (unable to synthesize TA and OA) and tbh-1 (unable to synthesize OA) null mutants are hypersensitive to the slowing-down response upon food encounter, resembling starved worms. Moreover, exogenous TA and OA recover the increased slowing-down locomotion of tdc-1 null mutants, suggesting that both, TA and OA, antagonize 5HTdependent food-related behaviors. Furthermore, our calcium imaging recordings showed that the peak of activity of serotoninergic neurons (ADF and NSM) upon encountering food is significantly higher in a tdc-1 null mutant background. We also found that tdc-1 null mutants are hypersensitive to the paralysis produced by exogenous 5-HT. Therefore, we are using this assay to evaluate the paralysis of the different TA and OA null receptor mutants to identify which receptors are involved in this antagonism. Taken together, these results suggest that TA and OA counteract with 5- HT by driving opposing food-related behaviors and by inhibiting 5-HT release
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195364
Neural modulation of foraging strategies in C. elegans; II Latin American Worm Meeting; Rosario; Argentina; 2020; 17-17
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195364
identifier_str_mv Neural modulation of foraging strategies in C. elegans; II Latin American Worm Meeting; Rosario; Argentina; 2020; 17-17
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://acacimesfe.org/actividades/reuniones/reuniones-2020/2nd-latin-american-worm-meeting-february-19-21-2020-rosario/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Latin American Worm Meeting
publisher.none.fl_str_mv Latin American Worm Meeting
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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