Serotonin and catecholamines neuronal circuits regulate opposing behaviors in Caenorhabditis elegans

Autores
Blanco, Maria Gabriela; Giunti, Sebastián; Rayes, Diego Hernán; de Rosa, Maria Jose
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Patients with anxiety disorders, such as post-traumatic stress disorders (PTSD) and panic attacks, exhibit high levels of catecholamines (CA), even in the absence of stress. Selective serotonin (5- HT) reuptake inhibitors (SSRIs), which increase the 5-HT level in the synaptic gap, are the most suitable drugs to treat these patients. This means 5-HT plays an important role in these disorders, but its relationship with CA is still unknown and difficult to study in the complex human nervous system. Given its simplicity and the highly conserved neurological pathways, C. elegans can be used to provide insights into the crosstalk between 5-TH and CA. When C. elegans encounters food, it releases 5-HT to inhibit locomotion. We exposed tdc-1 and tbh-1 null mutants (unable to synthetize the analogous of mammalian CA tyramine (TA) and octopamine (OA), respectively) to exogenous 5-HT and found that they are hypersensitive to paralysis. These results strongly suggest that 5-HT acts antagonistically to CA. In addition, we studied the hypersensitivity to exogenous 5-HT of mutants in TA and OA receptors. We observed that tyra-3, ser-3 and ser-6 null mutants do not recover completely from the serotonin-induced paralysis. We are now digging into the molecular and cellular underpinning of these antagonistic effects by analyzing mutants in 5-HT receptors. These opposite actions could be conserved in mammals and explain the efficiency of SSRIs in PTSD and panic attack treatments.
Fil: Blanco, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Giunti, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
XXXIII Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias
Córdoba
Argentina
Sociedad Argentina de Investigación en Neurociencias
Materia
C. ELEGANS
SEROTONIN
TYRAMINE
ANTAGONISTIC EFFECTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/218175
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/218175
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Serotonin and catecholamines neuronal circuits regulate opposing behaviors in Caenorhabditis elegansBlanco, Maria GabrielaGiunti, SebastiánRayes, Diego Hernánde Rosa, Maria JoseC. ELEGANSSEROTONINTYRAMINEANTAGONISTIC EFFECTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Patients with anxiety disorders, such as post-traumatic stress disorders (PTSD) and panic attacks, exhibit high levels of catecholamines (CA), even in the absence of stress. Selective serotonin (5- HT) reuptake inhibitors (SSRIs), which increase the 5-HT level in the synaptic gap, are the most suitable drugs to treat these patients. This means 5-HT plays an important role in these disorders, but its relationship with CA is still unknown and difficult to study in the complex human nervous system. Given its simplicity and the highly conserved neurological pathways, C. elegans can be used to provide insights into the crosstalk between 5-TH and CA. When C. elegans encounters food, it releases 5-HT to inhibit locomotion. We exposed tdc-1 and tbh-1 null mutants (unable to synthetize the analogous of mammalian CA tyramine (TA) and octopamine (OA), respectively) to exogenous 5-HT and found that they are hypersensitive to paralysis. These results strongly suggest that 5-HT acts antagonistically to CA. In addition, we studied the hypersensitivity to exogenous 5-HT of mutants in TA and OA receptors. We observed that tyra-3, ser-3 and ser-6 null mutants do not recover completely from the serotonin-induced paralysis. We are now digging into the molecular and cellular underpinning of these antagonistic effects by analyzing mutants in 5-HT receptors. These opposite actions could be conserved in mammals and explain the efficiency of SSRIs in PTSD and panic attack treatments.Fil: Blanco, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Giunti, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaXXXIII Reunión Anual de la Sociedad Argentina de Investigación en NeurocienciasCórdobaArgentinaSociedad Argentina de Investigación en NeurocienciasUniversidad Nacional de Córdoba2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/218175Serotonin and catecholamines neuronal circuits regulate opposing behaviors in Caenorhabditis elegans; XXXIII Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; Córdoba; Argentina; 2018; 97-97CONICET DigitalCONICETengNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:11:40Zoai:ri.conicet.gov.ar:11336/218175instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:11:40.669CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Serotonin and catecholamines neuronal circuits regulate opposing behaviors in Caenorhabditis elegans
title Serotonin and catecholamines neuronal circuits regulate opposing behaviors in Caenorhabditis elegans
spellingShingle Serotonin and catecholamines neuronal circuits regulate opposing behaviors in Caenorhabditis elegans
Blanco, Maria Gabriela
C. ELEGANS
SEROTONIN
TYRAMINE
ANTAGONISTIC EFFECTS
title_short Serotonin and catecholamines neuronal circuits regulate opposing behaviors in Caenorhabditis elegans
title_full Serotonin and catecholamines neuronal circuits regulate opposing behaviors in Caenorhabditis elegans
title_fullStr Serotonin and catecholamines neuronal circuits regulate opposing behaviors in Caenorhabditis elegans
title_full_unstemmed Serotonin and catecholamines neuronal circuits regulate opposing behaviors in Caenorhabditis elegans
title_sort Serotonin and catecholamines neuronal circuits regulate opposing behaviors in Caenorhabditis elegans
dc.creator.none.fl_str_mv Blanco, Maria Gabriela
Giunti, Sebastián
Rayes, Diego Hernán
de Rosa, Maria Jose
author Blanco, Maria Gabriela
author_facet Blanco, Maria Gabriela
Giunti, Sebastián
Rayes, Diego Hernán
de Rosa, Maria Jose
author_role author
author2 Giunti, Sebastián
Rayes, Diego Hernán
de Rosa, Maria Jose
author2_role author
author
author
dc.subject.none.fl_str_mv C. ELEGANS
SEROTONIN
TYRAMINE
ANTAGONISTIC EFFECTS
topic C. ELEGANS
SEROTONIN
TYRAMINE
ANTAGONISTIC EFFECTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Patients with anxiety disorders, such as post-traumatic stress disorders (PTSD) and panic attacks, exhibit high levels of catecholamines (CA), even in the absence of stress. Selective serotonin (5- HT) reuptake inhibitors (SSRIs), which increase the 5-HT level in the synaptic gap, are the most suitable drugs to treat these patients. This means 5-HT plays an important role in these disorders, but its relationship with CA is still unknown and difficult to study in the complex human nervous system. Given its simplicity and the highly conserved neurological pathways, C. elegans can be used to provide insights into the crosstalk between 5-TH and CA. When C. elegans encounters food, it releases 5-HT to inhibit locomotion. We exposed tdc-1 and tbh-1 null mutants (unable to synthetize the analogous of mammalian CA tyramine (TA) and octopamine (OA), respectively) to exogenous 5-HT and found that they are hypersensitive to paralysis. These results strongly suggest that 5-HT acts antagonistically to CA. In addition, we studied the hypersensitivity to exogenous 5-HT of mutants in TA and OA receptors. We observed that tyra-3, ser-3 and ser-6 null mutants do not recover completely from the serotonin-induced paralysis. We are now digging into the molecular and cellular underpinning of these antagonistic effects by analyzing mutants in 5-HT receptors. These opposite actions could be conserved in mammals and explain the efficiency of SSRIs in PTSD and panic attack treatments.
Fil: Blanco, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Giunti, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rayes, Diego Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: de Rosa, Maria Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
XXXIII Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias
Córdoba
Argentina
Sociedad Argentina de Investigación en Neurociencias
description Patients with anxiety disorders, such as post-traumatic stress disorders (PTSD) and panic attacks, exhibit high levels of catecholamines (CA), even in the absence of stress. Selective serotonin (5- HT) reuptake inhibitors (SSRIs), which increase the 5-HT level in the synaptic gap, are the most suitable drugs to treat these patients. This means 5-HT plays an important role in these disorders, but its relationship with CA is still unknown and difficult to study in the complex human nervous system. Given its simplicity and the highly conserved neurological pathways, C. elegans can be used to provide insights into the crosstalk between 5-TH and CA. When C. elegans encounters food, it releases 5-HT to inhibit locomotion. We exposed tdc-1 and tbh-1 null mutants (unable to synthetize the analogous of mammalian CA tyramine (TA) and octopamine (OA), respectively) to exogenous 5-HT and found that they are hypersensitive to paralysis. These results strongly suggest that 5-HT acts antagonistically to CA. In addition, we studied the hypersensitivity to exogenous 5-HT of mutants in TA and OA receptors. We observed that tyra-3, ser-3 and ser-6 null mutants do not recover completely from the serotonin-induced paralysis. We are now digging into the molecular and cellular underpinning of these antagonistic effects by analyzing mutants in 5-HT receptors. These opposite actions could be conserved in mammals and explain the efficiency of SSRIs in PTSD and panic attack treatments.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/218175
Serotonin and catecholamines neuronal circuits regulate opposing behaviors in Caenorhabditis elegans; XXXIII Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; Córdoba; Argentina; 2018; 97-97
CONICET Digital
CONICET
url http://hdl.handle.net/11336/218175
identifier_str_mv Serotonin and catecholamines neuronal circuits regulate opposing behaviors in Caenorhabditis elegans; XXXIII Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; Córdoba; Argentina; 2018; 97-97
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Universidad Nacional de Córdoba
publisher.none.fl_str_mv Universidad Nacional de Córdoba
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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