Detección de Microcystis y microcistina mediante la Reacción en Cadena de la Polimerasa

Autores
Chávez Luzanía, Roel Alejandro; Olea Félix, Jennifer; Alonso Rodríguez, Rosalba; Hernando, Marcelo Pablo; De los Santos Villalobos, Sergio
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cyanobacteria are photosynthetic organisms important in multiple biosphere life cycles; however, genera such as Microcystis have the ability to form blooms and produce cyanotoxins that compromise water quality This study aimed to validate the use of endpoint PCR to detect cyanobacteria, the genus Microcystis, and their genetic ability to produce microcystins. Three oligonucleotide pairs were used for the 16S rRNA gene of cyanobacteria and Microcystis, as well as mcyA. The use of end-point PCR allowed the specific detection of cyanobacteria and Microcystis in cell concentrations up to 10 times below the bloom alert limit, as well as the detection of the mcyA gene, both in isolated strains and in a simulated microbial community. The effectiveness of using end-point PCR for the specific detection of cyanobacteria makes it an early monitoring tool, capable of predicting potential microcystin production and thus contributing significantly to water quality management.
Fil: Chávez Luzanía, Roel Alejandro. Universidad Autónoma del Estado de México; México
Fil: Olea Félix, Jennifer. Universidad Autónoma del Estado de México; México
Fil: Alonso Rodríguez, Rosalba. Universidad Autónoma del Estado de México; México
Fil: Hernando, Marcelo Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De los Santos Villalobos, Sergio. Universidad Autónoma del Estado de México; México
Materia
cianobacterias
cianotoxinas
mcyA
Microcystis aeruginosa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/247169
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detección de Microcystis y microcistina mediante la Reacción en Cadena de la PolimerasaMicrocystis aeruginosa and microcystin detection by Polymerase Chain ReactionChávez Luzanía, Roel AlejandroOlea Félix, JenniferAlonso Rodríguez, RosalbaHernando, Marcelo PabloDe los Santos Villalobos, SergiocianobacteriascianotoxinasmcyAMicrocystis aeruginosahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cyanobacteria are photosynthetic organisms important in multiple biosphere life cycles; however, genera such as Microcystis have the ability to form blooms and produce cyanotoxins that compromise water quality This study aimed to validate the use of endpoint PCR to detect cyanobacteria, the genus Microcystis, and their genetic ability to produce microcystins. Three oligonucleotide pairs were used for the 16S rRNA gene of cyanobacteria and Microcystis, as well as mcyA. The use of end-point PCR allowed the specific detection of cyanobacteria and Microcystis in cell concentrations up to 10 times below the bloom alert limit, as well as the detection of the mcyA gene, both in isolated strains and in a simulated microbial community. The effectiveness of using end-point PCR for the specific detection of cyanobacteria makes it an early monitoring tool, capable of predicting potential microcystin production and thus contributing significantly to water quality management.Fil: Chávez Luzanía, Roel Alejandro. Universidad Autónoma del Estado de México; MéxicoFil: Olea Félix, Jennifer. Universidad Autónoma del Estado de México; MéxicoFil: Alonso Rodríguez, Rosalba. Universidad Autónoma del Estado de México; MéxicoFil: Hernando, Marcelo Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: De los Santos Villalobos, Sergio. Universidad Autónoma del Estado de México; MéxicoUniversidad Autónoma de Nayarit2024-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/247169Chávez Luzanía, Roel Alejandro; Olea Félix, Jennifer; Alonso Rodríguez, Rosalba; Hernando, Marcelo Pablo; De los Santos Villalobos, Sergio; Detección de Microcystis y microcistina mediante la Reacción en Cadena de la Polimerasa; Universidad Autónoma de Nayarit; Revista Bio Ciencias; 9-2024; 1-122007-3380CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://revistabiociencias.uan.edu.mx/index.php/BIOCIENCIAS/article/view/1758info:eu-repo/semantics/altIdentifier/doi/10.15741/revbio.11.e1758info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:31Zoai:ri.conicet.gov.ar:11336/247169instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:31.629CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detección de Microcystis y microcistina mediante la Reacción en Cadena de la Polimerasa
Microcystis aeruginosa and microcystin detection by Polymerase Chain Reaction
title Detección de Microcystis y microcistina mediante la Reacción en Cadena de la Polimerasa
spellingShingle Detección de Microcystis y microcistina mediante la Reacción en Cadena de la Polimerasa
Chávez Luzanía, Roel Alejandro
cianobacterias
cianotoxinas
mcyA
Microcystis aeruginosa
title_short Detección de Microcystis y microcistina mediante la Reacción en Cadena de la Polimerasa
title_full Detección de Microcystis y microcistina mediante la Reacción en Cadena de la Polimerasa
title_fullStr Detección de Microcystis y microcistina mediante la Reacción en Cadena de la Polimerasa
title_full_unstemmed Detección de Microcystis y microcistina mediante la Reacción en Cadena de la Polimerasa
title_sort Detección de Microcystis y microcistina mediante la Reacción en Cadena de la Polimerasa
dc.creator.none.fl_str_mv Chávez Luzanía, Roel Alejandro
Olea Félix, Jennifer
Alonso Rodríguez, Rosalba
Hernando, Marcelo Pablo
De los Santos Villalobos, Sergio
author Chávez Luzanía, Roel Alejandro
author_facet Chávez Luzanía, Roel Alejandro
Olea Félix, Jennifer
Alonso Rodríguez, Rosalba
Hernando, Marcelo Pablo
De los Santos Villalobos, Sergio
author_role author
author2 Olea Félix, Jennifer
Alonso Rodríguez, Rosalba
Hernando, Marcelo Pablo
De los Santos Villalobos, Sergio
author2_role author
author
author
author
dc.subject.none.fl_str_mv cianobacterias
cianotoxinas
mcyA
Microcystis aeruginosa
topic cianobacterias
cianotoxinas
mcyA
Microcystis aeruginosa
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cyanobacteria are photosynthetic organisms important in multiple biosphere life cycles; however, genera such as Microcystis have the ability to form blooms and produce cyanotoxins that compromise water quality This study aimed to validate the use of endpoint PCR to detect cyanobacteria, the genus Microcystis, and their genetic ability to produce microcystins. Three oligonucleotide pairs were used for the 16S rRNA gene of cyanobacteria and Microcystis, as well as mcyA. The use of end-point PCR allowed the specific detection of cyanobacteria and Microcystis in cell concentrations up to 10 times below the bloom alert limit, as well as the detection of the mcyA gene, both in isolated strains and in a simulated microbial community. The effectiveness of using end-point PCR for the specific detection of cyanobacteria makes it an early monitoring tool, capable of predicting potential microcystin production and thus contributing significantly to water quality management.
Fil: Chávez Luzanía, Roel Alejandro. Universidad Autónoma del Estado de México; México
Fil: Olea Félix, Jennifer. Universidad Autónoma del Estado de México; México
Fil: Alonso Rodríguez, Rosalba. Universidad Autónoma del Estado de México; México
Fil: Hernando, Marcelo Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De los Santos Villalobos, Sergio. Universidad Autónoma del Estado de México; México
description Cyanobacteria are photosynthetic organisms important in multiple biosphere life cycles; however, genera such as Microcystis have the ability to form blooms and produce cyanotoxins that compromise water quality This study aimed to validate the use of endpoint PCR to detect cyanobacteria, the genus Microcystis, and their genetic ability to produce microcystins. Three oligonucleotide pairs were used for the 16S rRNA gene of cyanobacteria and Microcystis, as well as mcyA. The use of end-point PCR allowed the specific detection of cyanobacteria and Microcystis in cell concentrations up to 10 times below the bloom alert limit, as well as the detection of the mcyA gene, both in isolated strains and in a simulated microbial community. The effectiveness of using end-point PCR for the specific detection of cyanobacteria makes it an early monitoring tool, capable of predicting potential microcystin production and thus contributing significantly to water quality management.
publishDate 2024
dc.date.none.fl_str_mv 2024-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/247169
Chávez Luzanía, Roel Alejandro; Olea Félix, Jennifer; Alonso Rodríguez, Rosalba; Hernando, Marcelo Pablo; De los Santos Villalobos, Sergio; Detección de Microcystis y microcistina mediante la Reacción en Cadena de la Polimerasa; Universidad Autónoma de Nayarit; Revista Bio Ciencias; 9-2024; 1-12
2007-3380
CONICET Digital
CONICET
url http://hdl.handle.net/11336/247169
identifier_str_mv Chávez Luzanía, Roel Alejandro; Olea Félix, Jennifer; Alonso Rodríguez, Rosalba; Hernando, Marcelo Pablo; De los Santos Villalobos, Sergio; Detección de Microcystis y microcistina mediante la Reacción en Cadena de la Polimerasa; Universidad Autónoma de Nayarit; Revista Bio Ciencias; 9-2024; 1-12
2007-3380
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://revistabiociencias.uan.edu.mx/index.php/BIOCIENCIAS/article/view/1758
info:eu-repo/semantics/altIdentifier/doi/10.15741/revbio.11.e1758
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidad Autónoma de Nayarit
publisher.none.fl_str_mv Universidad Autónoma de Nayarit
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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