Critical Effect of Oxygen Concentration and Acidity on the Efficiency of Photodegradation of Levofloxacin with Solar UVB Light; Cytotoxicity on Mammalian Cells of the Photoproducts...

Autores
Biondi, Macarena Agostina; Spesia, Mariana Belen; Sabini, Maria Carola; Biasutti, Maria Alicia; Montejano, Hernan Alfredo; Reynoso, Eugenia
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Levofloxacin is an antibiotic classified as an emerging contaminant. Its presence in aquatic environments represents potential risks to ecosystems and human health, making its removal during wastewater treatment of relevant importance. Here, we present a comprehensive kinetic analysis of levofloxacin photodegradation under UVB solar irradiation, with emphasis on the influence of pH and dissolved oxygen, two conditions that can vary widely in wastewater and impact treatment efficiency. We also investigated the formation and role of reactive oxygen species in the degradation mechanism, as well as the cytotoxicity and antibacterial activity of photoproducts. Our findings reveal that the efficiency of levofloxacin photodegradation is highly dependent on environmental conditions; it requires neutral or slightly alkaline pH and a high concentration of dissolved oxygen, a situation not always observed in contaminated waters. Several reactive oxygen species are generated, with singlet oxygen being the most reactive with the antibiotic. We report for the first time the singlet oxygen quantum yield from levofloxacin. Bioassays demonstrated that photoproducts neither exhibit antibacterial activity nor induce significant cytotoxicity. Our study suggests that UVB treatment of contaminated effluent containing levofloxacin could be an effective and environmentally safe strategy for the antibiotic degradation under certain conditions of pH and dissolved oxygen.
Fil: Biondi, Macarena Agostina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Spesia, Mariana Belen. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Sabini, Maria Carola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Biasutti, Maria Alicia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Montejano, Hernan Alfredo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Reynoso, Eugenia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Materia
PHOTODEGRADATION
SOLAR UVB LIGHT
LEVOFLOXACIN
PH
DISSOLVED OXYGEN
ANTIMICROBIAL ACTIVITY
CYTOTOXICTY
HIGHLY CONTAMINATED WASTEWATER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/278116
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/278116
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Critical Effect of Oxygen Concentration and Acidity on the Efficiency of Photodegradation of Levofloxacin with Solar UVB Light; Cytotoxicity on Mammalian Cells of the Photoproducts and Its Activity on Pathogenic BacteriaBiondi, Macarena AgostinaSpesia, Mariana BelenSabini, Maria CarolaBiasutti, Maria AliciaMontejano, Hernan AlfredoReynoso, EugeniaPHOTODEGRADATIONSOLAR UVB LIGHTLEVOFLOXACINPHDISSOLVED OXYGENANTIMICROBIAL ACTIVITYCYTOTOXICTYHIGHLY CONTAMINATED WASTEWATERhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Levofloxacin is an antibiotic classified as an emerging contaminant. Its presence in aquatic environments represents potential risks to ecosystems and human health, making its removal during wastewater treatment of relevant importance. Here, we present a comprehensive kinetic analysis of levofloxacin photodegradation under UVB solar irradiation, with emphasis on the influence of pH and dissolved oxygen, two conditions that can vary widely in wastewater and impact treatment efficiency. We also investigated the formation and role of reactive oxygen species in the degradation mechanism, as well as the cytotoxicity and antibacterial activity of photoproducts. Our findings reveal that the efficiency of levofloxacin photodegradation is highly dependent on environmental conditions; it requires neutral or slightly alkaline pH and a high concentration of dissolved oxygen, a situation not always observed in contaminated waters. Several reactive oxygen species are generated, with singlet oxygen being the most reactive with the antibiotic. We report for the first time the singlet oxygen quantum yield from levofloxacin. Bioassays demonstrated that photoproducts neither exhibit antibacterial activity nor induce significant cytotoxicity. Our study suggests that UVB treatment of contaminated effluent containing levofloxacin could be an effective and environmentally safe strategy for the antibiotic degradation under certain conditions of pH and dissolved oxygen.Fil: Biondi, Macarena Agostina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Spesia, Mariana Belen. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Sabini, Maria Carola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Biasutti, Maria Alicia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Montejano, Hernan Alfredo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Reynoso, Eugenia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaMultidisciplinary Digital Publishing Institute2025-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/278116Biondi, Macarena Agostina; Spesia, Mariana Belen; Sabini, Maria Carola; Biasutti, Maria Alicia; Montejano, Hernan Alfredo; et al.; Critical Effect of Oxygen Concentration and Acidity on the Efficiency of Photodegradation of Levofloxacin with Solar UVB Light; Cytotoxicity on Mammalian Cells of the Photoproducts and Its Activity on Pathogenic Bacteria; Multidisciplinary Digital Publishing Institute; Compounds; 5; 2; 6-2025; 1-202673-6918CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-6918/5/2/23info:eu-repo/semantics/altIdentifier/doi/10.3390/compounds5020023info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T14:08:20Zoai:ri.conicet.gov.ar:11336/278116instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 14:08:20.919CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Critical Effect of Oxygen Concentration and Acidity on the Efficiency of Photodegradation of Levofloxacin with Solar UVB Light; Cytotoxicity on Mammalian Cells of the Photoproducts and Its Activity on Pathogenic Bacteria
title Critical Effect of Oxygen Concentration and Acidity on the Efficiency of Photodegradation of Levofloxacin with Solar UVB Light; Cytotoxicity on Mammalian Cells of the Photoproducts and Its Activity on Pathogenic Bacteria
spellingShingle Critical Effect of Oxygen Concentration and Acidity on the Efficiency of Photodegradation of Levofloxacin with Solar UVB Light; Cytotoxicity on Mammalian Cells of the Photoproducts and Its Activity on Pathogenic Bacteria
Biondi, Macarena Agostina
PHOTODEGRADATION
SOLAR UVB LIGHT
LEVOFLOXACIN
PH
DISSOLVED OXYGEN
ANTIMICROBIAL ACTIVITY
CYTOTOXICTY
HIGHLY CONTAMINATED WASTEWATER
title_short Critical Effect of Oxygen Concentration and Acidity on the Efficiency of Photodegradation of Levofloxacin with Solar UVB Light; Cytotoxicity on Mammalian Cells of the Photoproducts and Its Activity on Pathogenic Bacteria
title_full Critical Effect of Oxygen Concentration and Acidity on the Efficiency of Photodegradation of Levofloxacin with Solar UVB Light; Cytotoxicity on Mammalian Cells of the Photoproducts and Its Activity on Pathogenic Bacteria
title_fullStr Critical Effect of Oxygen Concentration and Acidity on the Efficiency of Photodegradation of Levofloxacin with Solar UVB Light; Cytotoxicity on Mammalian Cells of the Photoproducts and Its Activity on Pathogenic Bacteria
title_full_unstemmed Critical Effect of Oxygen Concentration and Acidity on the Efficiency of Photodegradation of Levofloxacin with Solar UVB Light; Cytotoxicity on Mammalian Cells of the Photoproducts and Its Activity on Pathogenic Bacteria
title_sort Critical Effect of Oxygen Concentration and Acidity on the Efficiency of Photodegradation of Levofloxacin with Solar UVB Light; Cytotoxicity on Mammalian Cells of the Photoproducts and Its Activity on Pathogenic Bacteria
dc.creator.none.fl_str_mv Biondi, Macarena Agostina
Spesia, Mariana Belen
Sabini, Maria Carola
Biasutti, Maria Alicia
Montejano, Hernan Alfredo
Reynoso, Eugenia
author Biondi, Macarena Agostina
author_facet Biondi, Macarena Agostina
Spesia, Mariana Belen
Sabini, Maria Carola
Biasutti, Maria Alicia
Montejano, Hernan Alfredo
Reynoso, Eugenia
author_role author
author2 Spesia, Mariana Belen
Sabini, Maria Carola
Biasutti, Maria Alicia
Montejano, Hernan Alfredo
Reynoso, Eugenia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv PHOTODEGRADATION
SOLAR UVB LIGHT
LEVOFLOXACIN
PH
DISSOLVED OXYGEN
ANTIMICROBIAL ACTIVITY
CYTOTOXICTY
HIGHLY CONTAMINATED WASTEWATER
topic PHOTODEGRADATION
SOLAR UVB LIGHT
LEVOFLOXACIN
PH
DISSOLVED OXYGEN
ANTIMICROBIAL ACTIVITY
CYTOTOXICTY
HIGHLY CONTAMINATED WASTEWATER
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Levofloxacin is an antibiotic classified as an emerging contaminant. Its presence in aquatic environments represents potential risks to ecosystems and human health, making its removal during wastewater treatment of relevant importance. Here, we present a comprehensive kinetic analysis of levofloxacin photodegradation under UVB solar irradiation, with emphasis on the influence of pH and dissolved oxygen, two conditions that can vary widely in wastewater and impact treatment efficiency. We also investigated the formation and role of reactive oxygen species in the degradation mechanism, as well as the cytotoxicity and antibacterial activity of photoproducts. Our findings reveal that the efficiency of levofloxacin photodegradation is highly dependent on environmental conditions; it requires neutral or slightly alkaline pH and a high concentration of dissolved oxygen, a situation not always observed in contaminated waters. Several reactive oxygen species are generated, with singlet oxygen being the most reactive with the antibiotic. We report for the first time the singlet oxygen quantum yield from levofloxacin. Bioassays demonstrated that photoproducts neither exhibit antibacterial activity nor induce significant cytotoxicity. Our study suggests that UVB treatment of contaminated effluent containing levofloxacin could be an effective and environmentally safe strategy for the antibiotic degradation under certain conditions of pH and dissolved oxygen.
Fil: Biondi, Macarena Agostina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Spesia, Mariana Belen. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Sabini, Maria Carola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Biasutti, Maria Alicia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Montejano, Hernan Alfredo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Reynoso, Eugenia. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
description Levofloxacin is an antibiotic classified as an emerging contaminant. Its presence in aquatic environments represents potential risks to ecosystems and human health, making its removal during wastewater treatment of relevant importance. Here, we present a comprehensive kinetic analysis of levofloxacin photodegradation under UVB solar irradiation, with emphasis on the influence of pH and dissolved oxygen, two conditions that can vary widely in wastewater and impact treatment efficiency. We also investigated the formation and role of reactive oxygen species in the degradation mechanism, as well as the cytotoxicity and antibacterial activity of photoproducts. Our findings reveal that the efficiency of levofloxacin photodegradation is highly dependent on environmental conditions; it requires neutral or slightly alkaline pH and a high concentration of dissolved oxygen, a situation not always observed in contaminated waters. Several reactive oxygen species are generated, with singlet oxygen being the most reactive with the antibiotic. We report for the first time the singlet oxygen quantum yield from levofloxacin. Bioassays demonstrated that photoproducts neither exhibit antibacterial activity nor induce significant cytotoxicity. Our study suggests that UVB treatment of contaminated effluent containing levofloxacin could be an effective and environmentally safe strategy for the antibiotic degradation under certain conditions of pH and dissolved oxygen.
publishDate 2025
dc.date.none.fl_str_mv 2025-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/278116
Biondi, Macarena Agostina; Spesia, Mariana Belen; Sabini, Maria Carola; Biasutti, Maria Alicia; Montejano, Hernan Alfredo; et al.; Critical Effect of Oxygen Concentration and Acidity on the Efficiency of Photodegradation of Levofloxacin with Solar UVB Light; Cytotoxicity on Mammalian Cells of the Photoproducts and Its Activity on Pathogenic Bacteria; Multidisciplinary Digital Publishing Institute; Compounds; 5; 2; 6-2025; 1-20
2673-6918
CONICET Digital
CONICET
url http://hdl.handle.net/11336/278116
identifier_str_mv Biondi, Macarena Agostina; Spesia, Mariana Belen; Sabini, Maria Carola; Biasutti, Maria Alicia; Montejano, Hernan Alfredo; et al.; Critical Effect of Oxygen Concentration and Acidity on the Efficiency of Photodegradation of Levofloxacin with Solar UVB Light; Cytotoxicity on Mammalian Cells of the Photoproducts and Its Activity on Pathogenic Bacteria; Multidisciplinary Digital Publishing Institute; Compounds; 5; 2; 6-2025; 1-20
2673-6918
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-6918/5/2/23
info:eu-repo/semantics/altIdentifier/doi/10.3390/compounds5020023
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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