Development of antimicrobial hybrid mesoporous silver phosphate-pectin microspheres for control release of levofloxacin

Autores
Bayon, Bernardo; Bucala, Veronica; Castro, Guillermo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Pectin was used as nucleation agent in the synthesis of spherical mesoporous Ag3PO4 microspheres for the development of dual antimicrobial carrier containing a fluoroquinolone. The hybrid Ag3PO4-pectin microspheres were characterized by biophysical methods using optical, scanning and transmission electronic microscopies, X-ray diffraction and energy dispersive analysis, differential scanning calorimetry, laser diffraction, and molecular gas adsorption (BrunauereEmmetteTeller). Scanning electron microscopy (SEM) images of silver phosphate microspheres without pectin displayed heterogeneous surface, bimodal size distribution between 0.9-1.0 mm and 1.5-1.8 mm with 20% and 25% population yield respectively and high amount of salt detritus. Meanwhile, SEM microphotographies of silver phosphate microspheres synthesized in presence of pectin showed a drastic change of particle morphology, homogenous surface, narrow size particle distribution in the 1.3-1.5 mm range with 90% population yield, 20-30% pore size increase and without debris. X-ray diffraction analysis of silver phosphate microspheres showed the same crystal profile in presence or absence of pectin suggesting no changes in the crystalline structure of Ag3PO4 by the addition of the biopolymer was made. Effect of silver phosphate hybrid microspheres loaded with levofloxacin tested against Escherichia coli and Staphylococcus aureus showed strong bactericidal activity compared with the bacteriostatic effect of free levofloxacin. The results are suggesting that hybrid Ag3PO4epectin microspheres containing levofloxacin can be used as effective antimicrobial against several microorganisms, making them applicable to diverse medical devices and for antimicrobial control systems. The Ag3PO4-pectin hybrid microspheres are advantageous since they are synthesized by green chemistry methodology under standard laboratory conditions, do not requiring purification steps, the technique is highly reproducible and they are available for drug loading and specific target tailoring. The main advantage of Ag3PO4-pectin microspheres is the synergic antimicrobial activity of the silver ion and the antibiotic in the same microdevice acting as biocide matrix and also as carrier for levofloxacin..
Fil: Bayon, Bernardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Bucala, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Castro, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Materia
Levofloxacin
Pectin
Silver Phosphate
Antimicrobial Microspheres
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/26489
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/26489
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of antimicrobial hybrid mesoporous silver phosphate-pectin microspheres for control release of levofloxacinBayon, BernardoBucala, VeronicaCastro, GuillermoLevofloxacinPectinSilver PhosphateAntimicrobial Microsphereshttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Pectin was used as nucleation agent in the synthesis of spherical mesoporous Ag3PO4 microspheres for the development of dual antimicrobial carrier containing a fluoroquinolone. The hybrid Ag3PO4-pectin microspheres were characterized by biophysical methods using optical, scanning and transmission electronic microscopies, X-ray diffraction and energy dispersive analysis, differential scanning calorimetry, laser diffraction, and molecular gas adsorption (BrunauereEmmetteTeller). Scanning electron microscopy (SEM) images of silver phosphate microspheres without pectin displayed heterogeneous surface, bimodal size distribution between 0.9-1.0 mm and 1.5-1.8 mm with 20% and 25% population yield respectively and high amount of salt detritus. Meanwhile, SEM microphotographies of silver phosphate microspheres synthesized in presence of pectin showed a drastic change of particle morphology, homogenous surface, narrow size particle distribution in the 1.3-1.5 mm range with 90% population yield, 20-30% pore size increase and without debris. X-ray diffraction analysis of silver phosphate microspheres showed the same crystal profile in presence or absence of pectin suggesting no changes in the crystalline structure of Ag3PO4 by the addition of the biopolymer was made. Effect of silver phosphate hybrid microspheres loaded with levofloxacin tested against Escherichia coli and Staphylococcus aureus showed strong bactericidal activity compared with the bacteriostatic effect of free levofloxacin. The results are suggesting that hybrid Ag3PO4epectin microspheres containing levofloxacin can be used as effective antimicrobial against several microorganisms, making them applicable to diverse medical devices and for antimicrobial control systems. The Ag3PO4-pectin hybrid microspheres are advantageous since they are synthesized by green chemistry methodology under standard laboratory conditions, do not requiring purification steps, the technique is highly reproducible and they are available for drug loading and specific target tailoring. The main advantage of Ag3PO4-pectin microspheres is the synergic antimicrobial activity of the silver ion and the antibiotic in the same microdevice acting as biocide matrix and also as carrier for levofloxacin..Fil: Bayon, Bernardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Bucala, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaFil: Castro, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaElsevier Science2015-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/26489Bayon, Bernardo; Bucala, Veronica; Castro, Guillermo; Development of antimicrobial hybrid mesoporous silver phosphate-pectin microspheres for control release of levofloxacin; Elsevier Science; Microporous and Mesoporous Materials; 226; 30-12-2015; 71-781387-1811CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.micromeso.2015.12.041info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1387181115007064info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:01Zoai:ri.conicet.gov.ar:11336/26489instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:01.705CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of antimicrobial hybrid mesoporous silver phosphate-pectin microspheres for control release of levofloxacin
title Development of antimicrobial hybrid mesoporous silver phosphate-pectin microspheres for control release of levofloxacin
spellingShingle Development of antimicrobial hybrid mesoporous silver phosphate-pectin microspheres for control release of levofloxacin
Bayon, Bernardo
Levofloxacin
Pectin
Silver Phosphate
Antimicrobial Microspheres
title_short Development of antimicrobial hybrid mesoporous silver phosphate-pectin microspheres for control release of levofloxacin
title_full Development of antimicrobial hybrid mesoporous silver phosphate-pectin microspheres for control release of levofloxacin
title_fullStr Development of antimicrobial hybrid mesoporous silver phosphate-pectin microspheres for control release of levofloxacin
title_full_unstemmed Development of antimicrobial hybrid mesoporous silver phosphate-pectin microspheres for control release of levofloxacin
title_sort Development of antimicrobial hybrid mesoporous silver phosphate-pectin microspheres for control release of levofloxacin
dc.creator.none.fl_str_mv Bayon, Bernardo
Bucala, Veronica
Castro, Guillermo
author Bayon, Bernardo
author_facet Bayon, Bernardo
Bucala, Veronica
Castro, Guillermo
author_role author
author2 Bucala, Veronica
Castro, Guillermo
author2_role author
author
dc.subject.none.fl_str_mv Levofloxacin
Pectin
Silver Phosphate
Antimicrobial Microspheres
topic Levofloxacin
Pectin
Silver Phosphate
Antimicrobial Microspheres
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Pectin was used as nucleation agent in the synthesis of spherical mesoporous Ag3PO4 microspheres for the development of dual antimicrobial carrier containing a fluoroquinolone. The hybrid Ag3PO4-pectin microspheres were characterized by biophysical methods using optical, scanning and transmission electronic microscopies, X-ray diffraction and energy dispersive analysis, differential scanning calorimetry, laser diffraction, and molecular gas adsorption (BrunauereEmmetteTeller). Scanning electron microscopy (SEM) images of silver phosphate microspheres without pectin displayed heterogeneous surface, bimodal size distribution between 0.9-1.0 mm and 1.5-1.8 mm with 20% and 25% population yield respectively and high amount of salt detritus. Meanwhile, SEM microphotographies of silver phosphate microspheres synthesized in presence of pectin showed a drastic change of particle morphology, homogenous surface, narrow size particle distribution in the 1.3-1.5 mm range with 90% population yield, 20-30% pore size increase and without debris. X-ray diffraction analysis of silver phosphate microspheres showed the same crystal profile in presence or absence of pectin suggesting no changes in the crystalline structure of Ag3PO4 by the addition of the biopolymer was made. Effect of silver phosphate hybrid microspheres loaded with levofloxacin tested against Escherichia coli and Staphylococcus aureus showed strong bactericidal activity compared with the bacteriostatic effect of free levofloxacin. The results are suggesting that hybrid Ag3PO4epectin microspheres containing levofloxacin can be used as effective antimicrobial against several microorganisms, making them applicable to diverse medical devices and for antimicrobial control systems. The Ag3PO4-pectin hybrid microspheres are advantageous since they are synthesized by green chemistry methodology under standard laboratory conditions, do not requiring purification steps, the technique is highly reproducible and they are available for drug loading and specific target tailoring. The main advantage of Ag3PO4-pectin microspheres is the synergic antimicrobial activity of the silver ion and the antibiotic in the same microdevice acting as biocide matrix and also as carrier for levofloxacin..
Fil: Bayon, Bernardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Bucala, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Fil: Castro, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
description Pectin was used as nucleation agent in the synthesis of spherical mesoporous Ag3PO4 microspheres for the development of dual antimicrobial carrier containing a fluoroquinolone. The hybrid Ag3PO4-pectin microspheres were characterized by biophysical methods using optical, scanning and transmission electronic microscopies, X-ray diffraction and energy dispersive analysis, differential scanning calorimetry, laser diffraction, and molecular gas adsorption (BrunauereEmmetteTeller). Scanning electron microscopy (SEM) images of silver phosphate microspheres without pectin displayed heterogeneous surface, bimodal size distribution between 0.9-1.0 mm and 1.5-1.8 mm with 20% and 25% population yield respectively and high amount of salt detritus. Meanwhile, SEM microphotographies of silver phosphate microspheres synthesized in presence of pectin showed a drastic change of particle morphology, homogenous surface, narrow size particle distribution in the 1.3-1.5 mm range with 90% population yield, 20-30% pore size increase and without debris. X-ray diffraction analysis of silver phosphate microspheres showed the same crystal profile in presence or absence of pectin suggesting no changes in the crystalline structure of Ag3PO4 by the addition of the biopolymer was made. Effect of silver phosphate hybrid microspheres loaded with levofloxacin tested against Escherichia coli and Staphylococcus aureus showed strong bactericidal activity compared with the bacteriostatic effect of free levofloxacin. The results are suggesting that hybrid Ag3PO4epectin microspheres containing levofloxacin can be used as effective antimicrobial against several microorganisms, making them applicable to diverse medical devices and for antimicrobial control systems. The Ag3PO4-pectin hybrid microspheres are advantageous since they are synthesized by green chemistry methodology under standard laboratory conditions, do not requiring purification steps, the technique is highly reproducible and they are available for drug loading and specific target tailoring. The main advantage of Ag3PO4-pectin microspheres is the synergic antimicrobial activity of the silver ion and the antibiotic in the same microdevice acting as biocide matrix and also as carrier for levofloxacin..
publishDate 2015
dc.date.none.fl_str_mv 2015-12-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/26489
Bayon, Bernardo; Bucala, Veronica; Castro, Guillermo; Development of antimicrobial hybrid mesoporous silver phosphate-pectin microspheres for control release of levofloxacin; Elsevier Science; Microporous and Mesoporous Materials; 226; 30-12-2015; 71-78
1387-1811
CONICET Digital
CONICET
url http://hdl.handle.net/11336/26489
identifier_str_mv Bayon, Bernardo; Bucala, Veronica; Castro, Guillermo; Development of antimicrobial hybrid mesoporous silver phosphate-pectin microspheres for control release of levofloxacin; Elsevier Science; Microporous and Mesoporous Materials; 226; 30-12-2015; 71-78
1387-1811
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micromeso.2015.12.041
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1387181115007064
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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