Ferritin Decorated PLGA/Paclitaxel Loaded Nanoparticles Endowed with an Enhanced Toxicity Toward MCF-7 Breast Tumor Cells

Autores
Turino, Ludmila Noelia; Ruggiero, Maria R.; Stefanìa, Rachele; Cutrin, Juan C.; Aime, Silvio; Geninatti Crich, Simonetta
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
PolyLactic and Glycolic Acid nanoparticles (PLGA-NPs), coated with L-Ferritin,are exploited for the simultaneous delivery of paclitaxel and an amphiphilic Gd based MRI contrast agent into breast cancer cells (MCF7). L-Ferritin has been covalently conjugated to the external surface of PLGA-NPs exploiting NHS activated carboxylic groups. The results confirmed that nanoparticles decorated with L-Ferritin have many advantages with respect both albumin-decorated and non-decorated particles. Ferritin moieties endow PLGA-NPs with targeting capability, exploiting SCARA5 receptors overexpressed by these tumour cells, that results in an increased paclitaxel cytotoxicity. Moreover, protein coating increased nanoparticle stability thus reducing the fast and aspecific drug release before reaching the target. The theranostic potentiality of the nanoparticles has been demonstrated by evaluating the signal intensity enhancement on T1-weighted MRI images of labelled MCF7 cells. The results werecompared with that obtained with MDA cells used as negative control due to their lower SCARA5 expression.
Fil: Turino, Ludmila Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Ruggiero, Maria R.. Universita di Torino; Italia
Fil: Stefanìa, Rachele. Universita di Torino; Italia
Fil: Cutrin, Juan C.. Universita di Torino; Italia
Fil: Aime, Silvio. Universita di Torino; Italia
Fil: Geninatti Crich, Simonetta. Universita di Torino; Italia
Materia
POLYLACTIC AND GLYCOLIC (PLGA) NANOPARTICLES
FERRITIN
GD BASED CONTRAST AGENTS
MRI
PACLITAXEL
BREAST CANCER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20797
Acceder
id CONICETDig_4e23f7734dadda3016720461afcfbd2c
oai_identifier_str oai:ri.conicet.gov.ar:11336/20797
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ferritin Decorated PLGA/Paclitaxel Loaded Nanoparticles Endowed with an Enhanced Toxicity Toward MCF-7 Breast Tumor CellsTurino, Ludmila NoeliaRuggiero, Maria R.Stefanìa, RacheleCutrin, Juan C.Aime, SilvioGeninatti Crich, SimonettaPOLYLACTIC AND GLYCOLIC (PLGA) NANOPARTICLESFERRITINGD BASED CONTRAST AGENTSMRIPACLITAXELBREAST CANCERhttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2https://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2PolyLactic and Glycolic Acid nanoparticles (PLGA-NPs), coated with L-Ferritin,are exploited for the simultaneous delivery of paclitaxel and an amphiphilic Gd based MRI contrast agent into breast cancer cells (MCF7). L-Ferritin has been covalently conjugated to the external surface of PLGA-NPs exploiting NHS activated carboxylic groups. The results confirmed that nanoparticles decorated with L-Ferritin have many advantages with respect both albumin-decorated and non-decorated particles. Ferritin moieties endow PLGA-NPs with targeting capability, exploiting SCARA5 receptors overexpressed by these tumour cells, that results in an increased paclitaxel cytotoxicity. Moreover, protein coating increased nanoparticle stability thus reducing the fast and aspecific drug release before reaching the target. The theranostic potentiality of the nanoparticles has been demonstrated by evaluating the signal intensity enhancement on T1-weighted MRI images of labelled MCF7 cells. The results werecompared with that obtained with MDA cells used as negative control due to their lower SCARA5 expression.Fil: Turino, Ludmila Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; ArgentinaFil: Ruggiero, Maria R.. Universita di Torino; ItaliaFil: Stefanìa, Rachele. Universita di Torino; ItaliaFil: Cutrin, Juan C.. Universita di Torino; ItaliaFil: Aime, Silvio. Universita di Torino; ItaliaFil: Geninatti Crich, Simonetta. Universita di Torino; ItaliaAmerican Chemical Society2017-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20797Turino, Ludmila Noelia; Ruggiero, Maria R.; Stefanìa, Rachele; Cutrin, Juan C.; Aime, Silvio; et al.; Ferritin Decorated PLGA/Paclitaxel Loaded Nanoparticles Endowed with an Enhanced Toxicity Toward MCF-7 Breast Tumor Cells; American Chemical Society; Bioconjugate Chemistry; 28; 4; 3-2017; 1283-12901043-1802CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/acs.bioconjchem.7b00096info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.bioconjchem.7b00096info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:59Zoai:ri.conicet.gov.ar:11336/20797instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:59.651CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ferritin Decorated PLGA/Paclitaxel Loaded Nanoparticles Endowed with an Enhanced Toxicity Toward MCF-7 Breast Tumor Cells
title Ferritin Decorated PLGA/Paclitaxel Loaded Nanoparticles Endowed with an Enhanced Toxicity Toward MCF-7 Breast Tumor Cells
spellingShingle Ferritin Decorated PLGA/Paclitaxel Loaded Nanoparticles Endowed with an Enhanced Toxicity Toward MCF-7 Breast Tumor Cells
Turino, Ludmila Noelia
POLYLACTIC AND GLYCOLIC (PLGA) NANOPARTICLES
FERRITIN
GD BASED CONTRAST AGENTS
MRI
PACLITAXEL
BREAST CANCER
title_short Ferritin Decorated PLGA/Paclitaxel Loaded Nanoparticles Endowed with an Enhanced Toxicity Toward MCF-7 Breast Tumor Cells
title_full Ferritin Decorated PLGA/Paclitaxel Loaded Nanoparticles Endowed with an Enhanced Toxicity Toward MCF-7 Breast Tumor Cells
title_fullStr Ferritin Decorated PLGA/Paclitaxel Loaded Nanoparticles Endowed with an Enhanced Toxicity Toward MCF-7 Breast Tumor Cells
title_full_unstemmed Ferritin Decorated PLGA/Paclitaxel Loaded Nanoparticles Endowed with an Enhanced Toxicity Toward MCF-7 Breast Tumor Cells
title_sort Ferritin Decorated PLGA/Paclitaxel Loaded Nanoparticles Endowed with an Enhanced Toxicity Toward MCF-7 Breast Tumor Cells
dc.creator.none.fl_str_mv Turino, Ludmila Noelia
Ruggiero, Maria R.
Stefanìa, Rachele
Cutrin, Juan C.
Aime, Silvio
Geninatti Crich, Simonetta
author Turino, Ludmila Noelia
author_facet Turino, Ludmila Noelia
Ruggiero, Maria R.
Stefanìa, Rachele
Cutrin, Juan C.
Aime, Silvio
Geninatti Crich, Simonetta
author_role author
author2 Ruggiero, Maria R.
Stefanìa, Rachele
Cutrin, Juan C.
Aime, Silvio
Geninatti Crich, Simonetta
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv POLYLACTIC AND GLYCOLIC (PLGA) NANOPARTICLES
FERRITIN
GD BASED CONTRAST AGENTS
MRI
PACLITAXEL
BREAST CANCER
topic POLYLACTIC AND GLYCOLIC (PLGA) NANOPARTICLES
FERRITIN
GD BASED CONTRAST AGENTS
MRI
PACLITAXEL
BREAST CANCER
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv PolyLactic and Glycolic Acid nanoparticles (PLGA-NPs), coated with L-Ferritin,are exploited for the simultaneous delivery of paclitaxel and an amphiphilic Gd based MRI contrast agent into breast cancer cells (MCF7). L-Ferritin has been covalently conjugated to the external surface of PLGA-NPs exploiting NHS activated carboxylic groups. The results confirmed that nanoparticles decorated with L-Ferritin have many advantages with respect both albumin-decorated and non-decorated particles. Ferritin moieties endow PLGA-NPs with targeting capability, exploiting SCARA5 receptors overexpressed by these tumour cells, that results in an increased paclitaxel cytotoxicity. Moreover, protein coating increased nanoparticle stability thus reducing the fast and aspecific drug release before reaching the target. The theranostic potentiality of the nanoparticles has been demonstrated by evaluating the signal intensity enhancement on T1-weighted MRI images of labelled MCF7 cells. The results werecompared with that obtained with MDA cells used as negative control due to their lower SCARA5 expression.
Fil: Turino, Ludmila Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
Fil: Ruggiero, Maria R.. Universita di Torino; Italia
Fil: Stefanìa, Rachele. Universita di Torino; Italia
Fil: Cutrin, Juan C.. Universita di Torino; Italia
Fil: Aime, Silvio. Universita di Torino; Italia
Fil: Geninatti Crich, Simonetta. Universita di Torino; Italia
description PolyLactic and Glycolic Acid nanoparticles (PLGA-NPs), coated with L-Ferritin,are exploited for the simultaneous delivery of paclitaxel and an amphiphilic Gd based MRI contrast agent into breast cancer cells (MCF7). L-Ferritin has been covalently conjugated to the external surface of PLGA-NPs exploiting NHS activated carboxylic groups. The results confirmed that nanoparticles decorated with L-Ferritin have many advantages with respect both albumin-decorated and non-decorated particles. Ferritin moieties endow PLGA-NPs with targeting capability, exploiting SCARA5 receptors overexpressed by these tumour cells, that results in an increased paclitaxel cytotoxicity. Moreover, protein coating increased nanoparticle stability thus reducing the fast and aspecific drug release before reaching the target. The theranostic potentiality of the nanoparticles has been demonstrated by evaluating the signal intensity enhancement on T1-weighted MRI images of labelled MCF7 cells. The results werecompared with that obtained with MDA cells used as negative control due to their lower SCARA5 expression.
publishDate 2017
dc.date.none.fl_str_mv 2017-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20797
Turino, Ludmila Noelia; Ruggiero, Maria R.; Stefanìa, Rachele; Cutrin, Juan C.; Aime, Silvio; et al.; Ferritin Decorated PLGA/Paclitaxel Loaded Nanoparticles Endowed with an Enhanced Toxicity Toward MCF-7 Breast Tumor Cells; American Chemical Society; Bioconjugate Chemistry; 28; 4; 3-2017; 1283-1290
1043-1802
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20797
identifier_str_mv Turino, Ludmila Noelia; Ruggiero, Maria R.; Stefanìa, Rachele; Cutrin, Juan C.; Aime, Silvio; et al.; Ferritin Decorated PLGA/Paclitaxel Loaded Nanoparticles Endowed with an Enhanced Toxicity Toward MCF-7 Breast Tumor Cells; American Chemical Society; Bioconjugate Chemistry; 28; 4; 3-2017; 1283-1290
1043-1802
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/acs.bioconjchem.7b00096
info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.bioconjchem.7b00096
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614157680246784
score 13.070432

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