Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells

Autores
Geninatti Crich, S.; Cadenazzi, M.; Lanzardo, S.; Conti, L.; Ruiu, R.; Alberti, D.; Cavallo, F.; Cutrin, Juan Carlos; Aime, S.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work the selective uptake of native horse spleen ferritin and apoferritin loaded with MRI contrast agents has been assessed in human breast cancer cells (MCF-7 and MDA-MB-231). The higher expression of L-ferritin receptors (SCARA5) led to an enhanced uptake in MCF-7 as shown in T2 and T1 weighted MR images, respectively. The high efficiency of ferritin internalization in MCF-7 has been exploited for the simultaneous delivery of curcumin, a natural therapeutic molecule endowed with antineoplastic and anti-inflammatory action, and the MRI contrast agent Gd-HPDO3A. This theranostic system is able to treat selectively breast cancer cells over-expressing ferritin receptors. By entrapping in apoferritin both Gd-HPDO3A and curcumin, it was possible to deliver a therapeutic dose of 167 μg ml−1 (as calculated by MRI) of this natural drug to MCF-7 cells, thus obtaining a significant reduction of cell proliferation.
Fil: Geninatti Crich, S.. Universitã â  Di Torino; Italia
Fil: Cadenazzi, M.. Universitã â  Di Torino; Italia
Fil: Lanzardo, S.. Universitã â  Di Torino; Italia
Fil: Conti, L.. Universitã â  Di Torino; Italia
Fil: Ruiu, R.. Universitã â  Di Torino; Italia
Fil: Alberti, D.. Universitã â  Di Torino; Italia
Fil: Cavallo, F.. Universitã â  Di Torino; Italia
Fil: Cutrin, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); Argentina. Università  Di Torino; Italia
Fil: Aime, S.. Universitã â  Di Torino; Italia
Materia
Ferritin
Theranostic
Breast Cancer
Mri
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7875
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/7875
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cellsGeninatti Crich, S.Cadenazzi, M.Lanzardo, S.Conti, L.Ruiu, R.Alberti, D.Cavallo, F.Cutrin, Juan CarlosAime, S.FerritinTheranosticBreast CancerMrihttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2In this work the selective uptake of native horse spleen ferritin and apoferritin loaded with MRI contrast agents has been assessed in human breast cancer cells (MCF-7 and MDA-MB-231). The higher expression of L-ferritin receptors (SCARA5) led to an enhanced uptake in MCF-7 as shown in T2 and T1 weighted MR images, respectively. The high efficiency of ferritin internalization in MCF-7 has been exploited for the simultaneous delivery of curcumin, a natural therapeutic molecule endowed with antineoplastic and anti-inflammatory action, and the MRI contrast agent Gd-HPDO3A. This theranostic system is able to treat selectively breast cancer cells over-expressing ferritin receptors. By entrapping in apoferritin both Gd-HPDO3A and curcumin, it was possible to deliver a therapeutic dose of 167 μg ml−1 (as calculated by MRI) of this natural drug to MCF-7 cells, thus obtaining a significant reduction of cell proliferation.Fil: Geninatti Crich, S.. Universitã â  Di Torino; ItaliaFil: Cadenazzi, M.. Universitã â  Di Torino; ItaliaFil: Lanzardo, S.. Universitã â  Di Torino; ItaliaFil: Conti, L.. Universitã â  Di Torino; ItaliaFil: Ruiu, R.. Universitã â  Di Torino; ItaliaFil: Alberti, D.. Universitã â  Di Torino; ItaliaFil: Cavallo, F.. Universitã â  Di Torino; ItaliaFil: Cutrin, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); Argentina. Università  Di Torino; ItaliaFil: Aime, S.. Universitã â  Di Torino; ItaliaTaylor & Francis2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7875Geninatti Crich, S.; Cadenazzi, M.; Lanzardo, S.; Conti, L.; Ruiu, R.; et al.; Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells; Taylor & Francis; Nanoscale And Microscale Thermophysical Engineering; 7; 15; 3-2015; 6527-65331556-7265enginfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2015/NR/C5NR00352K#!divAbstractinfo:eu-repo/semantics/altIdentifier/doi/10.1039/C5NR00352Kinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:32:30Zoai:ri.conicet.gov.ar:11336/7875instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:32:31.163CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells
title Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells
spellingShingle Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells
Geninatti Crich, S.
Ferritin
Theranostic
Breast Cancer
Mri
title_short Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells
title_full Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells
title_fullStr Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells
title_full_unstemmed Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells
title_sort Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells
dc.creator.none.fl_str_mv Geninatti Crich, S.
Cadenazzi, M.
Lanzardo, S.
Conti, L.
Ruiu, R.
Alberti, D.
Cavallo, F.
Cutrin, Juan Carlos
Aime, S.
author Geninatti Crich, S.
author_facet Geninatti Crich, S.
Cadenazzi, M.
Lanzardo, S.
Conti, L.
Ruiu, R.
Alberti, D.
Cavallo, F.
Cutrin, Juan Carlos
Aime, S.
author_role author
author2 Cadenazzi, M.
Lanzardo, S.
Conti, L.
Ruiu, R.
Alberti, D.
Cavallo, F.
Cutrin, Juan Carlos
Aime, S.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ferritin
Theranostic
Breast Cancer
Mri
topic Ferritin
Theranostic
Breast Cancer
Mri
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv In this work the selective uptake of native horse spleen ferritin and apoferritin loaded with MRI contrast agents has been assessed in human breast cancer cells (MCF-7 and MDA-MB-231). The higher expression of L-ferritin receptors (SCARA5) led to an enhanced uptake in MCF-7 as shown in T2 and T1 weighted MR images, respectively. The high efficiency of ferritin internalization in MCF-7 has been exploited for the simultaneous delivery of curcumin, a natural therapeutic molecule endowed with antineoplastic and anti-inflammatory action, and the MRI contrast agent Gd-HPDO3A. This theranostic system is able to treat selectively breast cancer cells over-expressing ferritin receptors. By entrapping in apoferritin both Gd-HPDO3A and curcumin, it was possible to deliver a therapeutic dose of 167 μg ml−1 (as calculated by MRI) of this natural drug to MCF-7 cells, thus obtaining a significant reduction of cell proliferation.
Fil: Geninatti Crich, S.. Universitã â  Di Torino; Italia
Fil: Cadenazzi, M.. Universitã â  Di Torino; Italia
Fil: Lanzardo, S.. Universitã â  Di Torino; Italia
Fil: Conti, L.. Universitã â  Di Torino; Italia
Fil: Ruiu, R.. Universitã â  Di Torino; Italia
Fil: Alberti, D.. Universitã â  Di Torino; Italia
Fil: Cavallo, F.. Universitã â  Di Torino; Italia
Fil: Cutrin, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Cardiológicas (i); Argentina. Università  Di Torino; Italia
Fil: Aime, S.. Universitã â  Di Torino; Italia
description In this work the selective uptake of native horse spleen ferritin and apoferritin loaded with MRI contrast agents has been assessed in human breast cancer cells (MCF-7 and MDA-MB-231). The higher expression of L-ferritin receptors (SCARA5) led to an enhanced uptake in MCF-7 as shown in T2 and T1 weighted MR images, respectively. The high efficiency of ferritin internalization in MCF-7 has been exploited for the simultaneous delivery of curcumin, a natural therapeutic molecule endowed with antineoplastic and anti-inflammatory action, and the MRI contrast agent Gd-HPDO3A. This theranostic system is able to treat selectively breast cancer cells over-expressing ferritin receptors. By entrapping in apoferritin both Gd-HPDO3A and curcumin, it was possible to deliver a therapeutic dose of 167 μg ml−1 (as calculated by MRI) of this natural drug to MCF-7 cells, thus obtaining a significant reduction of cell proliferation.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7875
Geninatti Crich, S.; Cadenazzi, M.; Lanzardo, S.; Conti, L.; Ruiu, R.; et al.; Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells; Taylor & Francis; Nanoscale And Microscale Thermophysical Engineering; 7; 15; 3-2015; 6527-6533
1556-7265
url http://hdl.handle.net/11336/7875
identifier_str_mv Geninatti Crich, S.; Cadenazzi, M.; Lanzardo, S.; Conti, L.; Ruiu, R.; et al.; Targeting ferritin receptors for the selective delivery of imaging and therapeutic agents to breast cancer cells; Taylor & Francis; Nanoscale And Microscale Thermophysical Engineering; 7; 15; 3-2015; 6527-6533
1556-7265
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2015/NR/C5NR00352K#!divAbstract
info:eu-repo/semantics/altIdentifier/doi/10.1039/C5NR00352K
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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