Soybean Ferritin: Isolation, Characterization, and Free Radical Generation F

Autores
Galatro, Andrea Verónica; Robello, Elizabeth; Puntarulo, Susana Angela
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The main aim of this work was to assess the multi-task role of ferritin (Ft) in the oxidative metabolism of soybean (Glycine max). Soybean seeds incubated for 24 h yielded 41 ± 5 microg Ft/g fresh weight. The rate of in vitro incorporation of iron (Fe) into Ft was tested by supplementing the reaction medium with physiological Fe chelators. The control rate, observed in the presence of 100 microM Fe, was not significantly different from the values observed in the presence of 100 microM Fe-his. However, it was significantly higher in the presence of 100 microM Fe-citrate (approximately 4.5-fold) or of 100 microM Fe-ATP (approximately 14-fold). Moreover, a substantial decrease in the Trp-dependent fluorescence of the Ft protein was determined during Fe uptake from Fe-citrate, as compared with the control. On the other hand, Ft addition to homogenates from soybean embryonic axes reduced endogenously generated ascorbyl radical, according to its capacity for Fe uptake. The data presented here suggest that Ft could be involved in the generation of free radicals, such as hydroxyl radical, by Fe-catalyzed reactions. Moreover, the scavenging of these radicals by Ft itself could then lead to protein damage. However, Ft could also prevent cellular damage by the uptake of catalytically active Fe.
Fil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Robello, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Puntarulo, Susana Angela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Materia
FERRITIN
IRON
SOYBEAN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/268596
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/268596
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Soybean Ferritin: Isolation, Characterization, and Free Radical Generation FGalatro, Andrea VerónicaRobello, ElizabethPuntarulo, Susana AngelaFERRITINIRONSOYBEANhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The main aim of this work was to assess the multi-task role of ferritin (Ft) in the oxidative metabolism of soybean (Glycine max). Soybean seeds incubated for 24 h yielded 41 ± 5 microg Ft/g fresh weight. The rate of in vitro incorporation of iron (Fe) into Ft was tested by supplementing the reaction medium with physiological Fe chelators. The control rate, observed in the presence of 100 microM Fe, was not significantly different from the values observed in the presence of 100 microM Fe-his. However, it was significantly higher in the presence of 100 microM Fe-citrate (approximately 4.5-fold) or of 100 microM Fe-ATP (approximately 14-fold). Moreover, a substantial decrease in the Trp-dependent fluorescence of the Ft protein was determined during Fe uptake from Fe-citrate, as compared with the control. On the other hand, Ft addition to homogenates from soybean embryonic axes reduced endogenously generated ascorbyl radical, according to its capacity for Fe uptake. The data presented here suggest that Ft could be involved in the generation of free radicals, such as hydroxyl radical, by Fe-catalyzed reactions. Moreover, the scavenging of these radicals by Ft itself could then lead to protein damage. However, Ft could also prevent cellular damage by the uptake of catalytically active Fe.Fil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; ArgentinaFil: Robello, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; ArgentinaFil: Puntarulo, Susana Angela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; ArgentinaWiley Blackwell Publishing, Inc2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268596Galatro, Andrea Verónica; Robello, Elizabeth; Puntarulo, Susana Angela; Soybean Ferritin: Isolation, Characterization, and Free Radical Generation F; Wiley Blackwell Publishing, Inc; Journal Of Integrative Plant Biology; 54; 1; 1-2012; 45-541672-9072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1744-7909.2011.01091.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1744-7909.2011.01091.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:03Zoai:ri.conicet.gov.ar:11336/268596instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:03.283CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Soybean Ferritin: Isolation, Characterization, and Free Radical Generation F
title Soybean Ferritin: Isolation, Characterization, and Free Radical Generation F
spellingShingle Soybean Ferritin: Isolation, Characterization, and Free Radical Generation F
Galatro, Andrea Verónica
FERRITIN
IRON
SOYBEAN
title_short Soybean Ferritin: Isolation, Characterization, and Free Radical Generation F
title_full Soybean Ferritin: Isolation, Characterization, and Free Radical Generation F
title_fullStr Soybean Ferritin: Isolation, Characterization, and Free Radical Generation F
title_full_unstemmed Soybean Ferritin: Isolation, Characterization, and Free Radical Generation F
title_sort Soybean Ferritin: Isolation, Characterization, and Free Radical Generation F
dc.creator.none.fl_str_mv Galatro, Andrea Verónica
Robello, Elizabeth
Puntarulo, Susana Angela
author Galatro, Andrea Verónica
author_facet Galatro, Andrea Verónica
Robello, Elizabeth
Puntarulo, Susana Angela
author_role author
author2 Robello, Elizabeth
Puntarulo, Susana Angela
author2_role author
author
dc.subject.none.fl_str_mv FERRITIN
IRON
SOYBEAN
topic FERRITIN
IRON
SOYBEAN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The main aim of this work was to assess the multi-task role of ferritin (Ft) in the oxidative metabolism of soybean (Glycine max). Soybean seeds incubated for 24 h yielded 41 ± 5 microg Ft/g fresh weight. The rate of in vitro incorporation of iron (Fe) into Ft was tested by supplementing the reaction medium with physiological Fe chelators. The control rate, observed in the presence of 100 microM Fe, was not significantly different from the values observed in the presence of 100 microM Fe-his. However, it was significantly higher in the presence of 100 microM Fe-citrate (approximately 4.5-fold) or of 100 microM Fe-ATP (approximately 14-fold). Moreover, a substantial decrease in the Trp-dependent fluorescence of the Ft protein was determined during Fe uptake from Fe-citrate, as compared with the control. On the other hand, Ft addition to homogenates from soybean embryonic axes reduced endogenously generated ascorbyl radical, according to its capacity for Fe uptake. The data presented here suggest that Ft could be involved in the generation of free radicals, such as hydroxyl radical, by Fe-catalyzed reactions. Moreover, the scavenging of these radicals by Ft itself could then lead to protein damage. However, Ft could also prevent cellular damage by the uptake of catalytically active Fe.
Fil: Galatro, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Robello, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
Fil: Puntarulo, Susana Angela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina
description The main aim of this work was to assess the multi-task role of ferritin (Ft) in the oxidative metabolism of soybean (Glycine max). Soybean seeds incubated for 24 h yielded 41 ± 5 microg Ft/g fresh weight. The rate of in vitro incorporation of iron (Fe) into Ft was tested by supplementing the reaction medium with physiological Fe chelators. The control rate, observed in the presence of 100 microM Fe, was not significantly different from the values observed in the presence of 100 microM Fe-his. However, it was significantly higher in the presence of 100 microM Fe-citrate (approximately 4.5-fold) or of 100 microM Fe-ATP (approximately 14-fold). Moreover, a substantial decrease in the Trp-dependent fluorescence of the Ft protein was determined during Fe uptake from Fe-citrate, as compared with the control. On the other hand, Ft addition to homogenates from soybean embryonic axes reduced endogenously generated ascorbyl radical, according to its capacity for Fe uptake. The data presented here suggest that Ft could be involved in the generation of free radicals, such as hydroxyl radical, by Fe-catalyzed reactions. Moreover, the scavenging of these radicals by Ft itself could then lead to protein damage. However, Ft could also prevent cellular damage by the uptake of catalytically active Fe.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/268596
Galatro, Andrea Verónica; Robello, Elizabeth; Puntarulo, Susana Angela; Soybean Ferritin: Isolation, Characterization, and Free Radical Generation F; Wiley Blackwell Publishing, Inc; Journal Of Integrative Plant Biology; 54; 1; 1-2012; 45-54
1672-9072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/268596
identifier_str_mv Galatro, Andrea Verónica; Robello, Elizabeth; Puntarulo, Susana Angela; Soybean Ferritin: Isolation, Characterization, and Free Radical Generation F; Wiley Blackwell Publishing, Inc; Journal Of Integrative Plant Biology; 54; 1; 1-2012; 45-54
1672-9072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1744-7909.2011.01091.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1744-7909.2011.01091.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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