The molecular sensory machinery of a Chagas disease vector: Expression changes through imaginal moult and sexually dimorphic features

Autores
Latorre Estivalis, Jose Manuel; Robertson, Hugh M.; Walden, Kimberly K. O.; Ruiz, Jerônimo; Oliveira Gonçalves, Leilane; Guarneri, Alessandra Aparecida; Lorenzo, Marcelo Gustavo
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The triatomine bug Rhodnius prolixus is a main vector of Chagas disease, which affects several million people, mostly in Latin-America. Host searching, pheromone communication, and microclimatic preferences are aspects of its behaviour that depend on multimodal sensory inputs. The molecular bases of these sensory processes are largely unknown. The expression levels of genes transcribed in antennae were compared between 5 th instar larvae, and female and male adults by means of RNA-Seq. The antennae of R. prolixus showed increased expression of several chemosensory-related genes in imaginal bugs, while both sexes had similar expression patterns for most target genes. Few cases suggest involvement of target genes in sexually dimorphic functions. Most odorant and ionotropic receptor genes seemed to be expressed in all libraries. OBPs and CSPs showed very high expression levels. Other sensory-related genes such as TRPs, PPKs and mechanoreceptors had consistent levels of expression in all libraries. Our study characterises most of the sensory gene repertoire of these insects, opening an avenue for functional genetics studies. The increase in expression of chemosensory genes suggests an enhanced role in adult bugs. This knowledge allows developing new behaviour interfering strategies, increasing the options for translational research in the vector control field.
Fil: Latorre Estivalis, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Fundación Oswaldo Cruz; Brasil
Fil: Robertson, Hugh M.. University of Illinois at Urbana; Estados Unidos
Fil: Walden, Kimberly K. O.. University of Illinois at Urbana; Estados Unidos
Fil: Ruiz, Jerônimo. Fundación Oswaldo Cruz; Brasil
Fil: Oliveira Gonçalves, Leilane. Fundación Oswaldo Cruz; Brasil
Fil: Guarneri, Alessandra Aparecida. Fundación Oswaldo Cruz; Brasil
Fil: Lorenzo, Marcelo Gustavo. Fundación Oswaldo Cruz; Brasil
Materia
RHODNIUS PROLIXUS
TRANSCRIPTOMICA
RECEPTORES SENSORIALES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/78155
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/78155
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling The molecular sensory machinery of a Chagas disease vector: Expression changes through imaginal moult and sexually dimorphic featuresLatorre Estivalis, Jose ManuelRobertson, Hugh M.Walden, Kimberly K. O.Ruiz, JerônimoOliveira Gonçalves, LeilaneGuarneri, Alessandra AparecidaLorenzo, Marcelo GustavoRHODNIUS PROLIXUSTRANSCRIPTOMICARECEPTORES SENSORIALEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The triatomine bug Rhodnius prolixus is a main vector of Chagas disease, which affects several million people, mostly in Latin-America. Host searching, pheromone communication, and microclimatic preferences are aspects of its behaviour that depend on multimodal sensory inputs. The molecular bases of these sensory processes are largely unknown. The expression levels of genes transcribed in antennae were compared between 5 th instar larvae, and female and male adults by means of RNA-Seq. The antennae of R. prolixus showed increased expression of several chemosensory-related genes in imaginal bugs, while both sexes had similar expression patterns for most target genes. Few cases suggest involvement of target genes in sexually dimorphic functions. Most odorant and ionotropic receptor genes seemed to be expressed in all libraries. OBPs and CSPs showed very high expression levels. Other sensory-related genes such as TRPs, PPKs and mechanoreceptors had consistent levels of expression in all libraries. Our study characterises most of the sensory gene repertoire of these insects, opening an avenue for functional genetics studies. The increase in expression of chemosensory genes suggests an enhanced role in adult bugs. This knowledge allows developing new behaviour interfering strategies, increasing the options for translational research in the vector control field.Fil: Latorre Estivalis, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Fundación Oswaldo Cruz; BrasilFil: Robertson, Hugh M.. University of Illinois at Urbana; Estados UnidosFil: Walden, Kimberly K. O.. University of Illinois at Urbana; Estados UnidosFil: Ruiz, Jerônimo. Fundación Oswaldo Cruz; BrasilFil: Oliveira Gonçalves, Leilane. Fundación Oswaldo Cruz; BrasilFil: Guarneri, Alessandra Aparecida. Fundación Oswaldo Cruz; BrasilFil: Lorenzo, Marcelo Gustavo. Fundación Oswaldo Cruz; BrasilNature Publishing Group2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/78155Latorre Estivalis, Jose Manuel; Robertson, Hugh M.; Walden, Kimberly K. O.; Ruiz, Jerônimo; Oliveira Gonçalves, Leilane; et al.; The molecular sensory machinery of a Chagas disease vector: Expression changes through imaginal moult and sexually dimorphic features; Nature Publishing Group; Scientific Reports; 7; 1-2017; 1-162045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/srep40049info:eu-repo/semantics/altIdentifier/doi/10.1038/srep40049info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:34:32Zoai:ri.conicet.gov.ar:11336/78155instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:34:32.717CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The molecular sensory machinery of a Chagas disease vector: Expression changes through imaginal moult and sexually dimorphic features
title The molecular sensory machinery of a Chagas disease vector: Expression changes through imaginal moult and sexually dimorphic features
spellingShingle The molecular sensory machinery of a Chagas disease vector: Expression changes through imaginal moult and sexually dimorphic features
Latorre Estivalis, Jose Manuel
RHODNIUS PROLIXUS
TRANSCRIPTOMICA
RECEPTORES SENSORIALES
title_short The molecular sensory machinery of a Chagas disease vector: Expression changes through imaginal moult and sexually dimorphic features
title_full The molecular sensory machinery of a Chagas disease vector: Expression changes through imaginal moult and sexually dimorphic features
title_fullStr The molecular sensory machinery of a Chagas disease vector: Expression changes through imaginal moult and sexually dimorphic features
title_full_unstemmed The molecular sensory machinery of a Chagas disease vector: Expression changes through imaginal moult and sexually dimorphic features
title_sort The molecular sensory machinery of a Chagas disease vector: Expression changes through imaginal moult and sexually dimorphic features
dc.creator.none.fl_str_mv Latorre Estivalis, Jose Manuel
Robertson, Hugh M.
Walden, Kimberly K. O.
Ruiz, Jerônimo
Oliveira Gonçalves, Leilane
Guarneri, Alessandra Aparecida
Lorenzo, Marcelo Gustavo
author Latorre Estivalis, Jose Manuel
author_facet Latorre Estivalis, Jose Manuel
Robertson, Hugh M.
Walden, Kimberly K. O.
Ruiz, Jerônimo
Oliveira Gonçalves, Leilane
Guarneri, Alessandra Aparecida
Lorenzo, Marcelo Gustavo
author_role author
author2 Robertson, Hugh M.
Walden, Kimberly K. O.
Ruiz, Jerônimo
Oliveira Gonçalves, Leilane
Guarneri, Alessandra Aparecida
Lorenzo, Marcelo Gustavo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv RHODNIUS PROLIXUS
TRANSCRIPTOMICA
RECEPTORES SENSORIALES
topic RHODNIUS PROLIXUS
TRANSCRIPTOMICA
RECEPTORES SENSORIALES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The triatomine bug Rhodnius prolixus is a main vector of Chagas disease, which affects several million people, mostly in Latin-America. Host searching, pheromone communication, and microclimatic preferences are aspects of its behaviour that depend on multimodal sensory inputs. The molecular bases of these sensory processes are largely unknown. The expression levels of genes transcribed in antennae were compared between 5 th instar larvae, and female and male adults by means of RNA-Seq. The antennae of R. prolixus showed increased expression of several chemosensory-related genes in imaginal bugs, while both sexes had similar expression patterns for most target genes. Few cases suggest involvement of target genes in sexually dimorphic functions. Most odorant and ionotropic receptor genes seemed to be expressed in all libraries. OBPs and CSPs showed very high expression levels. Other sensory-related genes such as TRPs, PPKs and mechanoreceptors had consistent levels of expression in all libraries. Our study characterises most of the sensory gene repertoire of these insects, opening an avenue for functional genetics studies. The increase in expression of chemosensory genes suggests an enhanced role in adult bugs. This knowledge allows developing new behaviour interfering strategies, increasing the options for translational research in the vector control field.
Fil: Latorre Estivalis, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Centro Regional de Estudios Genómicos; Argentina. Fundación Oswaldo Cruz; Brasil
Fil: Robertson, Hugh M.. University of Illinois at Urbana; Estados Unidos
Fil: Walden, Kimberly K. O.. University of Illinois at Urbana; Estados Unidos
Fil: Ruiz, Jerônimo. Fundación Oswaldo Cruz; Brasil
Fil: Oliveira Gonçalves, Leilane. Fundación Oswaldo Cruz; Brasil
Fil: Guarneri, Alessandra Aparecida. Fundación Oswaldo Cruz; Brasil
Fil: Lorenzo, Marcelo Gustavo. Fundación Oswaldo Cruz; Brasil
description The triatomine bug Rhodnius prolixus is a main vector of Chagas disease, which affects several million people, mostly in Latin-America. Host searching, pheromone communication, and microclimatic preferences are aspects of its behaviour that depend on multimodal sensory inputs. The molecular bases of these sensory processes are largely unknown. The expression levels of genes transcribed in antennae were compared between 5 th instar larvae, and female and male adults by means of RNA-Seq. The antennae of R. prolixus showed increased expression of several chemosensory-related genes in imaginal bugs, while both sexes had similar expression patterns for most target genes. Few cases suggest involvement of target genes in sexually dimorphic functions. Most odorant and ionotropic receptor genes seemed to be expressed in all libraries. OBPs and CSPs showed very high expression levels. Other sensory-related genes such as TRPs, PPKs and mechanoreceptors had consistent levels of expression in all libraries. Our study characterises most of the sensory gene repertoire of these insects, opening an avenue for functional genetics studies. The increase in expression of chemosensory genes suggests an enhanced role in adult bugs. This knowledge allows developing new behaviour interfering strategies, increasing the options for translational research in the vector control field.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/78155
Latorre Estivalis, Jose Manuel; Robertson, Hugh M.; Walden, Kimberly K. O.; Ruiz, Jerônimo; Oliveira Gonçalves, Leilane; et al.; The molecular sensory machinery of a Chagas disease vector: Expression changes through imaginal moult and sexually dimorphic features; Nature Publishing Group; Scientific Reports; 7; 1-2017; 1-16
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/78155
identifier_str_mv Latorre Estivalis, Jose Manuel; Robertson, Hugh M.; Walden, Kimberly K. O.; Ruiz, Jerônimo; Oliveira Gonçalves, Leilane; et al.; The molecular sensory machinery of a Chagas disease vector: Expression changes through imaginal moult and sexually dimorphic features; Nature Publishing Group; Scientific Reports; 7; 1-2017; 1-16
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/srep40049
info:eu-repo/semantics/altIdentifier/doi/10.1038/srep40049
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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