The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features

Autores
Latorre Estivalis, José Manuel; Robertson, H. M.; Walden, K. K. O.; Ruiz, J.; Gonçalves, L.O.; Guarneri, A. A.; Lorenzo, M. G.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The triatomine bug Rhodnius prolixus is a main vector of Chagas disease, which affects several million people, mostly in Latin-America. Host searching, pheromone communication, and microclimatic preferences are aspects of its behaviour that depend on multimodal sensory inputs. The molecular bases of these sensory processes are largely unknown. The expression levels of genes transcribed in antennae were compared between 5th instar larvae, and female and male adults by means of RNA-Seq. The antennae of R. prolixus showed increased expression of several chemosensory-related genes in imaginal bugs, while both sexes had similar expression patterns for most target genes. Few cases suggest involvement of target genes in sexually dimorphic functions. Most odorant and ionotropic receptor genes seemed to be expressed in all libraries. OBPs and CSPs showed very high expression levels. Other sensory-related genes such as TRPs, PPKs and mechanoreceptors had consistent levels of expression in all libraries. Our study characterises most of the sensory gene repertoire of these insects, opening an avenue for functional genetics studies. The increase in expression of chemosensory genes suggests an enhanced role in adult bugs. This knowledge allows developing new behaviour interfering strategies, increasing the options for translational research in the vector control field.
Centro Regional de Estudios Genómicos
Materia
Ciencias Exactas
Biología
Chagas disease
Rhodnius prolixus
Sensory-related genes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/87711
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/87711
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic featuresLatorre Estivalis, José ManuelRobertson, H. M.Walden, K. K. O.Ruiz, J.Gonçalves, L.O.Guarneri, A. A.Lorenzo, M. G.Ciencias ExactasBiologíaChagas diseaseRhodnius prolixusSensory-related genesThe triatomine bug Rhodnius prolixus is a main vector of Chagas disease, which affects several million people, mostly in Latin-America. Host searching, pheromone communication, and microclimatic preferences are aspects of its behaviour that depend on multimodal sensory inputs. The molecular bases of these sensory processes are largely unknown. The expression levels of genes transcribed in antennae were compared between 5<sup>th</sup> instar larvae, and female and male adults by means of RNA-Seq. The antennae of R. prolixus showed increased expression of several chemosensory-related genes in imaginal bugs, while both sexes had similar expression patterns for most target genes. Few cases suggest involvement of target genes in sexually dimorphic functions. Most odorant and ionotropic receptor genes seemed to be expressed in all libraries. OBPs and CSPs showed very high expression levels. Other sensory-related genes such as TRPs, PPKs and mechanoreceptors had consistent levels of expression in all libraries. Our study characterises most of the sensory gene repertoire of these insects, opening an avenue for functional genetics studies. The increase in expression of chemosensory genes suggests an enhanced role in adult bugs. This knowledge allows developing new behaviour interfering strategies, increasing the options for translational research in the vector control field.Centro Regional de Estudios Genómicos2017-01-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/87711enginfo:eu-repo/semantics/altIdentifier/issn/2045-2322info:eu-repo/semantics/altIdentifier/doi/10.1038/srep40049info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:17:15Zoai:sedici.unlp.edu.ar:10915/87711Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:17:15.347SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features
title The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features
spellingShingle The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features
Latorre Estivalis, José Manuel
Ciencias Exactas
Biología
Chagas disease
Rhodnius prolixus
Sensory-related genes
title_short The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features
title_full The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features
title_fullStr The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features
title_full_unstemmed The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features
title_sort The molecular sensory machinery of a Chagas disease vector: expression changes through imaginal moult and sexually dimorphic features
dc.creator.none.fl_str_mv Latorre Estivalis, José Manuel
Robertson, H. M.
Walden, K. K. O.
Ruiz, J.
Gonçalves, L.O.
Guarneri, A. A.
Lorenzo, M. G.
author Latorre Estivalis, José Manuel
author_facet Latorre Estivalis, José Manuel
Robertson, H. M.
Walden, K. K. O.
Ruiz, J.
Gonçalves, L.O.
Guarneri, A. A.
Lorenzo, M. G.
author_role author
author2 Robertson, H. M.
Walden, K. K. O.
Ruiz, J.
Gonçalves, L.O.
Guarneri, A. A.
Lorenzo, M. G.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
Chagas disease
Rhodnius prolixus
Sensory-related genes
topic Ciencias Exactas
Biología
Chagas disease
Rhodnius prolixus
Sensory-related genes
dc.description.none.fl_txt_mv The triatomine bug Rhodnius prolixus is a main vector of Chagas disease, which affects several million people, mostly in Latin-America. Host searching, pheromone communication, and microclimatic preferences are aspects of its behaviour that depend on multimodal sensory inputs. The molecular bases of these sensory processes are largely unknown. The expression levels of genes transcribed in antennae were compared between 5<sup>th</sup> instar larvae, and female and male adults by means of RNA-Seq. The antennae of R. prolixus showed increased expression of several chemosensory-related genes in imaginal bugs, while both sexes had similar expression patterns for most target genes. Few cases suggest involvement of target genes in sexually dimorphic functions. Most odorant and ionotropic receptor genes seemed to be expressed in all libraries. OBPs and CSPs showed very high expression levels. Other sensory-related genes such as TRPs, PPKs and mechanoreceptors had consistent levels of expression in all libraries. Our study characterises most of the sensory gene repertoire of these insects, opening an avenue for functional genetics studies. The increase in expression of chemosensory genes suggests an enhanced role in adult bugs. This knowledge allows developing new behaviour interfering strategies, increasing the options for translational research in the vector control field.
Centro Regional de Estudios Genómicos
description The triatomine bug Rhodnius prolixus is a main vector of Chagas disease, which affects several million people, mostly in Latin-America. Host searching, pheromone communication, and microclimatic preferences are aspects of its behaviour that depend on multimodal sensory inputs. The molecular bases of these sensory processes are largely unknown. The expression levels of genes transcribed in antennae were compared between 5<sup>th</sup> instar larvae, and female and male adults by means of RNA-Seq. The antennae of R. prolixus showed increased expression of several chemosensory-related genes in imaginal bugs, while both sexes had similar expression patterns for most target genes. Few cases suggest involvement of target genes in sexually dimorphic functions. Most odorant and ionotropic receptor genes seemed to be expressed in all libraries. OBPs and CSPs showed very high expression levels. Other sensory-related genes such as TRPs, PPKs and mechanoreceptors had consistent levels of expression in all libraries. Our study characterises most of the sensory gene repertoire of these insects, opening an avenue for functional genetics studies. The increase in expression of chemosensory genes suggests an enhanced role in adult bugs. This knowledge allows developing new behaviour interfering strategies, increasing the options for translational research in the vector control field.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/87711
url http://sedici.unlp.edu.ar/handle/10915/87711
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2045-2322
info:eu-repo/semantics/altIdentifier/doi/10.1038/srep40049
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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