Global characterization of gene expression in the brain of starved immature Rhodnius prolixus

Autores
Coraiola Nevoa, Jessica; Latorre Estivalis, Jose Manuel; Sviatopolk Mirsky Pais, Fabiano; Pinto Marliere, Newmar; da Rocha Fernandes, Gabriel; Lorenzo, Marcelo Gustavo; Aparecida Guarneri, Alessandra
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background Rhodnius prolixus is a vector of Chagas disease and has become a model organism to study physiology, behavior, and pathogen interaction. The publication of its genome allowed initiating a process of comparative characterization of the gene expression profiles of diverse organs exposed to varying conditions. Brain processes control the expression of behavior and, as such, mediate immediate adjustment to a changing environment, allowing organisms to maximize their chances to survive and reproduce. The expression of fundamental behavioral processes like feeding requires fine control in triatomines because they obtain their blood meals from potential predators. Therefore, the characterization of gene expression profiles of key components modulating behavior in brain processes, like those of neuropeptide precursors and their receptors, seems fundamental. Here we study global gene expression profiles in the brain of starved R. prolixus fifth instar nymphs by means of RNA sequencing (RNA-Seq). Results The expression of neuromodulatory genes such as those of precursors of neuropeptides, neurohormones, and their receptors; as well as the enzymes involved in the biosynthesis and processing of neuropeptides and biogenic amines were fully characterized. Other important gene targets such as neurotransmitter receptors, nuclear receptors, clock genes, sensory receptors, and takeouts genes were identified and their gene expression analyzed. Conclusion We propose that the set of neuromodulatory-related genes highly expressed in the brain of starved R. prolixus nymphs deserves functional characterization to allow the subsequent development of tools targeting them for bug control. As the brain is a complex structure that presents functionally specialized areas, future studies should focus on characterizing gene expression profiles in target areas, e.g. mushroom bodies, to complement our current knowledge.
Fil: Coraiola Nevoa, Jessica. Fundación Oswaldo Cruz; Brasil
Fil: Latorre Estivalis, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Sviatopolk Mirsky Pais, Fabiano. Fundación Oswaldo Cruz; Brasil
Fil: Pinto Marliere, Newmar. Fundación Oswaldo Cruz; Brasil
Fil: da Rocha Fernandes, Gabriel. Fundación Oswaldo Cruz; Brasil
Fil: Lorenzo, Marcelo Gustavo. Fundación Oswaldo Cruz; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aparecida Guarneri, Alessandra. Fundación Oswaldo Cruz; Brasil
Materia
RHODNIUS PROLIXUS
BRAIN
TRANSCRIPTOME
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228004
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/228004
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Global characterization of gene expression in the brain of starved immature Rhodnius prolixusCoraiola Nevoa, JessicaLatorre Estivalis, Jose ManuelSviatopolk Mirsky Pais, FabianoPinto Marliere, Newmarda Rocha Fernandes, GabrielLorenzo, Marcelo GustavoAparecida Guarneri, AlessandraRHODNIUS PROLIXUSBRAINTRANSCRIPTOMEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background Rhodnius prolixus is a vector of Chagas disease and has become a model organism to study physiology, behavior, and pathogen interaction. The publication of its genome allowed initiating a process of comparative characterization of the gene expression profiles of diverse organs exposed to varying conditions. Brain processes control the expression of behavior and, as such, mediate immediate adjustment to a changing environment, allowing organisms to maximize their chances to survive and reproduce. The expression of fundamental behavioral processes like feeding requires fine control in triatomines because they obtain their blood meals from potential predators. Therefore, the characterization of gene expression profiles of key components modulating behavior in brain processes, like those of neuropeptide precursors and their receptors, seems fundamental. Here we study global gene expression profiles in the brain of starved R. prolixus fifth instar nymphs by means of RNA sequencing (RNA-Seq). Results The expression of neuromodulatory genes such as those of precursors of neuropeptides, neurohormones, and their receptors; as well as the enzymes involved in the biosynthesis and processing of neuropeptides and biogenic amines were fully characterized. Other important gene targets such as neurotransmitter receptors, nuclear receptors, clock genes, sensory receptors, and takeouts genes were identified and their gene expression analyzed. Conclusion We propose that the set of neuromodulatory-related genes highly expressed in the brain of starved R. prolixus nymphs deserves functional characterization to allow the subsequent development of tools targeting them for bug control. As the brain is a complex structure that presents functionally specialized areas, future studies should focus on characterizing gene expression profiles in target areas, e.g. mushroom bodies, to complement our current knowledge.Fil: Coraiola Nevoa, Jessica. Fundación Oswaldo Cruz; BrasilFil: Latorre Estivalis, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Sviatopolk Mirsky Pais, Fabiano. Fundación Oswaldo Cruz; BrasilFil: Pinto Marliere, Newmar. Fundación Oswaldo Cruz; BrasilFil: da Rocha Fernandes, Gabriel. Fundación Oswaldo Cruz; BrasilFil: Lorenzo, Marcelo Gustavo. Fundación Oswaldo Cruz; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aparecida Guarneri, Alessandra. Fundación Oswaldo Cruz; BrasilPublic Library of Science2023-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228004Coraiola Nevoa, Jessica; Latorre Estivalis, Jose Manuel; Sviatopolk Mirsky Pais, Fabiano; Pinto Marliere, Newmar; da Rocha Fernandes, Gabriel; et al.; Global characterization of gene expression in the brain of starved immature Rhodnius prolixus; Public Library of Science; Plos One; 18; 3-2023; 1-181932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://dx.plos.org/10.1371/journal.pone.0282490info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0282490info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:11:02Zoai:ri.conicet.gov.ar:11336/228004instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:11:02.589CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Global characterization of gene expression in the brain of starved immature Rhodnius prolixus
title Global characterization of gene expression in the brain of starved immature Rhodnius prolixus
spellingShingle Global characterization of gene expression in the brain of starved immature Rhodnius prolixus
Coraiola Nevoa, Jessica
RHODNIUS PROLIXUS
BRAIN
TRANSCRIPTOME
title_short Global characterization of gene expression in the brain of starved immature Rhodnius prolixus
title_full Global characterization of gene expression in the brain of starved immature Rhodnius prolixus
title_fullStr Global characterization of gene expression in the brain of starved immature Rhodnius prolixus
title_full_unstemmed Global characterization of gene expression in the brain of starved immature Rhodnius prolixus
title_sort Global characterization of gene expression in the brain of starved immature Rhodnius prolixus
dc.creator.none.fl_str_mv Coraiola Nevoa, Jessica
Latorre Estivalis, Jose Manuel
Sviatopolk Mirsky Pais, Fabiano
Pinto Marliere, Newmar
da Rocha Fernandes, Gabriel
Lorenzo, Marcelo Gustavo
Aparecida Guarneri, Alessandra
author Coraiola Nevoa, Jessica
author_facet Coraiola Nevoa, Jessica
Latorre Estivalis, Jose Manuel
Sviatopolk Mirsky Pais, Fabiano
Pinto Marliere, Newmar
da Rocha Fernandes, Gabriel
Lorenzo, Marcelo Gustavo
Aparecida Guarneri, Alessandra
author_role author
author2 Latorre Estivalis, Jose Manuel
Sviatopolk Mirsky Pais, Fabiano
Pinto Marliere, Newmar
da Rocha Fernandes, Gabriel
Lorenzo, Marcelo Gustavo
Aparecida Guarneri, Alessandra
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv RHODNIUS PROLIXUS
BRAIN
TRANSCRIPTOME
topic RHODNIUS PROLIXUS
BRAIN
TRANSCRIPTOME
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background Rhodnius prolixus is a vector of Chagas disease and has become a model organism to study physiology, behavior, and pathogen interaction. The publication of its genome allowed initiating a process of comparative characterization of the gene expression profiles of diverse organs exposed to varying conditions. Brain processes control the expression of behavior and, as such, mediate immediate adjustment to a changing environment, allowing organisms to maximize their chances to survive and reproduce. The expression of fundamental behavioral processes like feeding requires fine control in triatomines because they obtain their blood meals from potential predators. Therefore, the characterization of gene expression profiles of key components modulating behavior in brain processes, like those of neuropeptide precursors and their receptors, seems fundamental. Here we study global gene expression profiles in the brain of starved R. prolixus fifth instar nymphs by means of RNA sequencing (RNA-Seq). Results The expression of neuromodulatory genes such as those of precursors of neuropeptides, neurohormones, and their receptors; as well as the enzymes involved in the biosynthesis and processing of neuropeptides and biogenic amines were fully characterized. Other important gene targets such as neurotransmitter receptors, nuclear receptors, clock genes, sensory receptors, and takeouts genes were identified and their gene expression analyzed. Conclusion We propose that the set of neuromodulatory-related genes highly expressed in the brain of starved R. prolixus nymphs deserves functional characterization to allow the subsequent development of tools targeting them for bug control. As the brain is a complex structure that presents functionally specialized areas, future studies should focus on characterizing gene expression profiles in target areas, e.g. mushroom bodies, to complement our current knowledge.
Fil: Coraiola Nevoa, Jessica. Fundación Oswaldo Cruz; Brasil
Fil: Latorre Estivalis, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Sviatopolk Mirsky Pais, Fabiano. Fundación Oswaldo Cruz; Brasil
Fil: Pinto Marliere, Newmar. Fundación Oswaldo Cruz; Brasil
Fil: da Rocha Fernandes, Gabriel. Fundación Oswaldo Cruz; Brasil
Fil: Lorenzo, Marcelo Gustavo. Fundación Oswaldo Cruz; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aparecida Guarneri, Alessandra. Fundación Oswaldo Cruz; Brasil
description Background Rhodnius prolixus is a vector of Chagas disease and has become a model organism to study physiology, behavior, and pathogen interaction. The publication of its genome allowed initiating a process of comparative characterization of the gene expression profiles of diverse organs exposed to varying conditions. Brain processes control the expression of behavior and, as such, mediate immediate adjustment to a changing environment, allowing organisms to maximize their chances to survive and reproduce. The expression of fundamental behavioral processes like feeding requires fine control in triatomines because they obtain their blood meals from potential predators. Therefore, the characterization of gene expression profiles of key components modulating behavior in brain processes, like those of neuropeptide precursors and their receptors, seems fundamental. Here we study global gene expression profiles in the brain of starved R. prolixus fifth instar nymphs by means of RNA sequencing (RNA-Seq). Results The expression of neuromodulatory genes such as those of precursors of neuropeptides, neurohormones, and their receptors; as well as the enzymes involved in the biosynthesis and processing of neuropeptides and biogenic amines were fully characterized. Other important gene targets such as neurotransmitter receptors, nuclear receptors, clock genes, sensory receptors, and takeouts genes were identified and their gene expression analyzed. Conclusion We propose that the set of neuromodulatory-related genes highly expressed in the brain of starved R. prolixus nymphs deserves functional characterization to allow the subsequent development of tools targeting them for bug control. As the brain is a complex structure that presents functionally specialized areas, future studies should focus on characterizing gene expression profiles in target areas, e.g. mushroom bodies, to complement our current knowledge.
publishDate 2023
dc.date.none.fl_str_mv 2023-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228004
Coraiola Nevoa, Jessica; Latorre Estivalis, Jose Manuel; Sviatopolk Mirsky Pais, Fabiano; Pinto Marliere, Newmar; da Rocha Fernandes, Gabriel; et al.; Global characterization of gene expression in the brain of starved immature Rhodnius prolixus; Public Library of Science; Plos One; 18; 3-2023; 1-18
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228004
identifier_str_mv Coraiola Nevoa, Jessica; Latorre Estivalis, Jose Manuel; Sviatopolk Mirsky Pais, Fabiano; Pinto Marliere, Newmar; da Rocha Fernandes, Gabriel; et al.; Global characterization of gene expression in the brain of starved immature Rhodnius prolixus; Public Library of Science; Plos One; 18; 3-2023; 1-18
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://dx.plos.org/10.1371/journal.pone.0282490
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0282490
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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